Medical image segmentation is a critical task in medical image analysis, and clustering algorithms can be utilized to achieve this goal. This research work focuses on the segmentation of neuro disorder magnetic resonance images using Otsu, K-means, and FCM coupled with the firefly optimization algorithm. Otsu is a classical thresholding algorithm that relies on a single threshold value to segment the images. K-Means is one of the simplest and most widely used clustering algorithms. It aims to partition data into K clusters, where each data point belongs to the cluster with the nearest mean. Fuzzy C-Means is an extension of K-Means, allowing data points to belong to multiple clusters with varying degrees of membership. In medical image segmentation, FCM was used to classify pixels or voxels into different tissue classes with soft boundaries, accounting for partial volume effects. Firefly Optimization helps in improving the convergence speed of the FCM algorithm. Firefly optimization is good at exploring the solution space and finding global optima. The combination of FCM and Firefly Optimization leads to more accurate clustering results. The performance evaluation was done by Renyi entropy and Shannon entropy, FCM coupled with the firefly optimization was found to exhibit superior results when compared with the Otsu and K-means clustering algorithms.