Developing non-grain forestry woody fuel industries such as Jatropha curcas L. (JCL) aligns with the national conditions of countries with food shortages and large populations, such as some countries in Africa and Asia, and is an ideal alternative to replace fossil fuels. Determining appropriate planting densities suitable for different regions is one of the most critical practices for agriculture and forestry to achieve high yields and sustainability. This study conducted a life cycle assessment (LCA) of the JCL biodiesel's environmental impact, energy balance, and economic analysis (3E) of two densities and three of the most suitable planting regions in China. Findings reveal that the density of 2500 plants/ha had a better 3E performance than 1600 plants/ha, and Yunnan province outperformed Guizhou province and Sichuan province. JCL biodiesel effectively mitigates the greenhouse effect (1141.14–1936.59 kg CO2 eq/t) compared to conventional fossil diesel in different cases. Key factors influencing environmental performance include seed yield, urea applied during cultivation, methanol for biodiesel conversion, and electricity for irrigation. The Net Energy Balance (NEB) and Net Energy Ratio (NER) demonstrate the superior energy efficiency of JCL biodiesel over fossil diesel. The cost of producing JCL biodiesel decreased with the maturation of the trees, with water use and labor salary accounting for over 80% due to the necessity of the cultivation stage. Although the Financial Net Present Value (FNPV) was negative when considering only JCL biodiesel, it became positive (12111.64–27342.44 CNY) with the inclusion of by-products (glycerine, press-cake, and shells) in high-density cases. Our findings suggest that JCL biodiesel with appropriate density can serve as a sustainable biofuel alternative, replacing a portion of the fossil diesel currently on the market and significantly contributing to climate change mitigation and nonrenewable energy conservation.
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