The chemical enrichment of the early Universe is a crucial element in the formation and evolution of galaxies, and Population III (Pop III) stars must play a vital role in this process. In this study, we examine metal enrichment from massive stars in the early Universe’s embryonic galaxies. Using radiation hydrodynamic simulations and stellar evolution modelling, we calculated the expected metal yield from these stars. Specifically, we applied accretion rates from a previous radiation-hydrodynamic simulation to inform our stellar evolution modelling, executed with the Geneva code, across 11 selected datasets, with final stellar masses between 500 and 9000 M⊙. Our results demonstrate that the first generation of Pop III stars within a mass range of 2000−9000 M⊙ result in N/O, C/O and O/H ratios compatible with the values observed in very high-z galaxies GN-z11 and CEERS 1019. The ejecta of these Pop III stars are predominantly composed of 4He, 1H, and 14N. Our Pop III chemical enrichment model of the halo can accurately reproduce the observed N/O and C/O ratios, and, by incorporating a hundred times more zero-metallicity interstellar material with the stellar ejecta, it accurately attains the observed O/H ratio. Thus, a sub-population of extremely massive Pop III stars, with masses surpassing approximately 2000 M⊙, effectively reproduces the CNO elemental abundances observed in high-z JWST galaxies to date. We closely reproduced the observed Ne/O ratio in CEERS 1019 employing a model with several thousand solar masses and non-zero metallicity, and we projected a 12C/13C ratio of 7, substantially lower than the solar ratio of around 90. The significant nitrogen enrichment predicted by Pop III stars with a few thousand solar masses not only reinforces the argument for a heavy seed formation pathway for massive black holes at redshifts as high as z = 10.6 but it also accentuates the need for deeper investigations into their complex nature and pivotal role in the early Universe.