Given the inherent difficulties in the teaching-learning process of molecular biology, active methodologies have been developed to ensure greater effectiveness. Prior research has indicated that these tools can enhance student performance across diverse areas, empowering students as protagonists in their learning processes. In this regard, to improve students' performance in the subject ZMV1482: Molecular Biology, the implementation of an alternative supplementary activity based on technological resources was proposed. The activity consisted of creating concise videos on digital platforms, covering the matter discussed in the preceding class and its application. Each week, three to four students undertook these presentations, followed by a discussion of the topic. The results obtained from the proposal were notably significant, with an average score for the complementary activity stood at 7.9 ± 2.80, which influenced the final course grade, which averaged at 7.1 ± 1.45. Furthermore, upon completing the course curriculum, an electronic survey was administered to 56.5% of the enrolled students. The results demonstrated that students were able to learn significantly with the proposed methodology and suggest that this approach exhibited superior efficacy compared to conventional forms of complementary activities, such as seminars. These findings support the notion that active and innovative methodologies should be encouraged in the field of molecular biology to enhance the understanding of the concepts covered throughout the course.