Filamentary Shape Research Articles

Cloud–Cloud Collision and Cluster Formation in the W5-NW Complex

We present a detailed structural and gas kinematic study of the star-forming complex W5-NW. A cloud–cloud collision scenario unravels with evidence of collision-induced star and cluster formation. Various signatures of cloud–cloud collision such as “complementary distribution” and “bridging features” are explored. At the colliding region, the two clouds have complementary morphologies, where W5-NWb has a filamentary key-like shape that fits into the U-shaped cavity in W5-NWa that behaves like a keyhole. The interaction region between the two clouds is characterized by bridging features with intermediate velocities connecting the two clouds. A skewed V-shaped bridging feature is also detected at the site of the collision. A robust picture of the molecular gas distribution highlighting the bridges is seen in the position–position–velocity diagram obtained using the SCOUSEPY algorithm. Star cluster formation with an overdensity of Class I and Class II young stellar objects is also seen towards this cloud complex, likely triggered by the cloud collision event.

Estimating cross-field particle transport at the outer midplane of TCV by tracking filaments with machine learning

Cross-field transport of particles in the boundary region of magnetically confined fusion plasmas is dominated by turbulence. Blobs, intermittent turbulent structures with large amplitude and a filamentary shape appearing in the scrape-off layer (SOL), are known from theoretical and experimental studies to be the main contributor to the cross-field particle transport. The dynamics of blobs differs depending on various plasma conditions, including triangularity (δ). In this work, we analyze triangularity dependence of the cross-field particle transport at the outer midplane of plasmas with , +0.15, −0.14, and −0.26 on the Tokamak à Configuration Variable, using our novel machine learning (ML) blob-tracking approach applied to gas puff imaging data. The cross-field particle flux determined in this way is of the same order as the overall transport inferred from KN1D, GBS, and SOLPS-ITER simulations, suggesting that the blobs identified by the ML blob-tracking account for most of the cross-field particle transport in the SOL. Also, the ML blob-tracking and KN1D show a decrease in the cross-field particle transport as δ becomes more negative. The blob-by-blob analysis of the result from the tracking reveals that the decrease of cross-field particle transport with decreasing δ is accompanied by a decrease in the number of blobs in a fixed time, which tend to have larger area and lower radial speed. Also, the blobs in these plasmas are in the connected sheath regime, and show a velocity scaling consistent with the two-region model.

Cellular patterns and dry convection in textured dust storms at the edge of Mars North Polar Cap

We present a study of textured local dust storms that develop at the northern polar cap boundary on Mars springtime. We have used images obtained with VMC and HRSC cameras onboard Mars Express and MARCI on MRO to analyze dust storms captured from March to July 2019 (Ls = 350° in MY 34–Ls = 54° in MY 35). The textured storms grow in the longitude sector 150°E-210°E centered at latitude ~60°N and exhibit spiral, filamentary and compact shapes that change and evolve rapidly in a daily basis. The storms translate by prevailing east and southeast winds with speeds 15–45 ms−1. In some areas of their interiors they show organized clusters of cells formed typically by 100 elements with sizes ~5–30 km with a length/width ratio ~ 1.2–3 in the wind direction. The cells have elongated downwind tails with lengths 4–8 times the cell size. The cells top altitudes are ~6–11 km above their surroundings. We propose that the spirals grow as baroclinic vortices within a vertically sheared eastward jet present at this epoch in Mars due to the intense meridional temperature gradient at the polar cap edge. We show using a simple one-dimensional model that the cells can be produced by shallow dry convection with dust acting as the heating source to generate the updrafts. These patterns resemble those seen in laboratory experiments and on clouds in Earth's atmosphere and can serve to comparatively elucidate and discern the different mechanisms at work in each case.

3D-printing of architectured short carbon fiber-geopolymer composite

Developing advanced lightweight structures with both high strength and high toughness remains challenging. Herein, we provide a combined experimental and simulation method to fabricate 3D-printed geopolymer complex structures with lightweight, high strength and superior toughness for the first time. In this research, short carbon fiber reinforced geopolymer (C sf GP) composites were printed using 3D printing technology, and the rheological properties of C sf GP inks and the mechanical properties of hardened geopolymer composite structures were systematically studied. The C sf GP inks exhibited obvious shear-thinning behavior, which facilitated ink-extrusion from a micro-nozzle, conserving the filamentary shape and supporting the subsequent printed layers. In C sf GP composites, the consistent orientation distribution of short carbon fibers primarily enhanced their mechanical properties. When fiber content was 3 wt %, flexural strength and compressive strength of the C sf GP composite were 309.2% and 375.8% higher than those of nonreinforced geopolymer, respectively. Subsequently, C sf GP composites of the Bouligand architectures were successfully 3D-printed, and they showed superior load-bearing capacity and non-brittle failure mode due to their hierarchically ordered architectures and sophisticated interfaces. 3D printing together with Bouligand-structure designing provide a novel approach for the C sf GP composites of lightweight, high strength and superior toughness, which would lead to a resurgence of interest in new lightweight structure design and manufacturing strategies. • Short carbon fiber reinforced geopolymer (C sf GP) inks were 3D printed by direct ink writing. • Consistent fiber orientation distribution was achieved in the printed C sf GP composites. • C sf GP of Bouligand structures were designed and printed, and the fracture behavior of these structures was investigated. • The obtained C sf GP of Bouligand structures showed light-weight, high strength and superior toughness, and non-brittle failure behavior.

Turning AGN Bubbles into Radio Relics with Sloshing: Modeling CR Transport with Realistic Physics

Radio relics are arc-like synchrotron sources at the periphery of galaxy clusters, produced by cosmic-ray electrons in a μG magnetic field, which are believed to have been (re-)accelerated by merger shock fronts. However, not all relics appear at the same location as shocks as seen in the X-ray. In a previous work, we suggested that the shape of some relics may result from the pre-existing spatial distribution of cosmic-ray electrons, and tested this hypothesis using simulations by launching AGN jets into a cluster atmosphere with sloshing gas motions generated by a previous merger event. We showed that these motions could transport the cosmic ray-enriched material of the AGN bubbles to large radii and stretch it in a tangential direction, producing a filamentary shape resembling a radio relic. In this work, we improve our physical description for the cosmic rays by modeling them as a separate fluid which undergoes diffusion and Alfvén losses. We find that, including this additional cosmic ray physics significantly diminishes the appearance of these filamentary features, showing that our original hypothesis is sensitive to the modeling of cosmic ray physics in the intracluster medium.

SdiA, a Quorum-Sensing Regulator, Suppresses Fimbriae Expression, Biofilm Formation, and Quorum-Sensing Signaling Molecules Production in Klebsiella pneumoniae

Klebsiella pneumoniae is a Gram-negative pathogen that has become a worldwide concern due to the emergence of multidrug-resistant isolates responsible for various invasive infectious diseases. Biofilm formation constitutes a major virulence factor for K. pneumoniae and relies on the expression of fimbrial adhesins and aggregation of bacterial cells on biotic or abiotic surfaces in a coordinated manner. During biofilm aggregation, bacterial cells communicate with each other through inter- or intra-species interactions mediated by signallng molecules, called autoinducers, in a mechanism known as quorum sensing (QS). In most Gram-negative bacteria, intra-species communication typically involves the LuxI/LuxR system: LuxI synthase produces N-acyl homoserine lactones (AHLs) as autoinducers and the LuxR transcription factor is their cognate receptor. However, K. pneumoniae does not produce AHL but encodes SdiA, an orphan LuxR-type receptor that responds to exogenous AHL molecules produced by other bacterial species. While SdiA regulates several cellular processes and the expression of virulence factors in many pathogens, the role of this regulator in K. pneumoniae remains unknown. In this study, we describe the characterization of sdiA mutant strain of K. pneumoniae. The sdiA mutant strain has increased biofilm formation, which correlates with the increased expression of type 1 fimbriae, thus revealing a repressive role of SdiA in fimbriae expression and bacterial cell adherence and aggregation. On the other hand, SdiA acts as a transcriptional activator of cell division machinery assembly in the septum, since cells lacking SdiA regulator exhibited a filamentary shape rather than the typical rod shape. We also show that K. pneumoniae cells lacking SdiA regulator present constant production of QS autoinducers at maximum levels, suggesting a putative role for SdiA in the regulation of AI-2 production. Taken together, our results demonstrate that SdiA regulates cell division and the expression of virulence factors such as fimbriae expression, biofilm formation, and production of QS autoinducers in K. pneumoniae.

Altered nuclear architecture in blood cells from Huntington's disease patients.

Cell nuclear architecture has been explored in cancer and laminopathies but not in neurodegenerative disorders. Huntington's disease (HD) is a neurodegenerative disorder that leads to neuronal death. Chromosome-wide changes in gene expression have been reported in HD, not only in the brain but also in peripheral blood cells, but whether this translates into nuclear and chromosome architecture alterations has not yet been studied. We investigate nuclear structure and chromosome organization in HD blood cells using fluorescence in situ hybridization in ultrathin cryosections (cryoFISH), coupled with machine learning image analysis to evaluate size, distribution, and morphology of nuclei and chromosomes. Four chromosomes were analyzed based on up- or downregulation of gene expression in HD. We show that blood cells from HD patients display increased nuclear size and filamentary shape, increased size of gene-rich chromosome 19, decreased filamentary shape of gene-rich chromosome 22, and a more radially centralized position for chromosome 19, whereas chromosomes 4 and 5 do not show detectable differences. We identify gross changes in nuclear architecture and chromosome organization associated with HD in blood. This adds a new layer of information onto disrupting mechanisms in HD and increases the potential of using blood to survey HD.

High-Performance Complementary Electrochromic Device Based on Iridium Oxide as a Counter Electrode.

In complementary electrochromic devices (ECDs), nickel oxide (NiO) is generally used as a counter electrode material for enhancing the coloration efficiency. However, an NiO film as a counter electrode in ECDs is susceptible to degradation upon prolonged electrochemical cycling, which leads to an insufficient device lifetime. In this study, a type of counter electrode iridium oxide (IrO2) layer was fabricated using vacuum cathodic arc plasma (CAP). We focused on the comparison of IrO2 and NiO deposited on a 5 × 5 cm2 indium tin oxide (ITO) glass substrate with various Ar/O2 gas-flow ratios (1/2, 1/2.5, and 1/3) in series. The optical performance of IrO2-ECD (glass/ITO/WO3/liquid electrolyte/IrO2/ITO/glass) was determined by optical transmittance modulation; ∆T = 50% (from Tbleaching (75%) to Tcoloring (25%)) at 633 nm was higher than that of NiO-ECD (ITO/NiO/liquid electrolyte/WO3/ITO) (∆T = 32%). Apart from this, the ECD device demonstrated a fast coloring time of 4.8 s, a bleaching time of 1.5 s, and good cycling durability, which remained at 50% transmittance modulation even after 1000 cycles. The fast time was associated with the IrO2 electrode and provided higher diffusion coefficients and a filamentary shape as an interface that facilitated the transfer of the Li ions into/out of the interface electrodes and the electrolyte. In our result of IrO2-ECD analyses, the higher optical transmittance modulation was useful for promoting electrochromic application to a cycle durability test as an alternative to NiO-ECD.

The Formation of an Atypical Sunspot Light Bridge as a Result of Large-scale Flux Emergence

Abstract We use a combination of full-disk data from the Solar Dynamics Observatory and high-resolution data from the Dunn Solar Telescope (DST) to study the formation, structure, and evolution of an atypical light bridge (LB) in a regular sunspot. The LB results from the emergence of magnetic flux with one footpoint rooted in a pore outside the parent sunspot that appears about 17 hr before the LB. The pore has a polarity opposite to that of the sunspot and recedes from it at a speed of about 0.4 km s−1. This is accompanied by the development of an elongated magnetic channel in the outer penumbra that triggers the formation of the LB when it reaches the inner penumbral boundary. The LB is a nearly horizontal structure with a field strength of about 1.2 kG that exhibits long-lived photospheric blueshifts of about 0.85 km s−1 along its entire length. The emergence of the LB leads to dynamic surges in the chromosphere and transition region about 13 minutes later. We derive the photospheric and chromospheric structure of the LB in the DST data from spectral line parameters and inversions of He i at 1083 nm, Si i at 1082.7 nm, Ca ii IR at 854 nm, and H α at 656 nm and speckle-reconstructed imaging at 700 and 430 nm. The LB shows an elongated filamentary shape in the photosphere without lateral extrusions. The thermal inversion of Ca ii IR reveals the LB to be about 600–800 K hotter than the umbra. Different sections of the LB are elevated to heights between 400 and 700 km. Our results indicate that LB formation is part of a flux emergence event with the LB envelope reaching a height of about 29 Mm before dissolving after about 13 hr. We conclude that the existence of persistent, large-scale photospheric blueshifts in LBs is the most likely criterion for distinguishing between flux emergence events and overturning convection in field-free umbral intrusions.

Characteristics of Ni- Cr Binary Alloys Produced by Conventional Powder Metallurgy

The characteristics of Ni-Cr porous alloys containing 0.5 wt%Cr up to 12 wt%Cr were determined. The alloys were prepared from a mix of Ni-particles with filamentary shape and Cr-particles with irregular shape which then mixed with 0.5 wt% paraffin wax as a binder. The samples were formed by compaction and then sintered at 1200 °C for 1 h. The microstructure of samples was found to consist of a Ni-Cr solid solution matrix with X-ray energy dispersive analysis of Cr% less than 0.33 wt%. The matrix microstructure which composed of equiaxed grains (20-60 μm) surrounded high Cr content islands which also contained γ phase (Ni2.88 Cr1.22). The apparent density of the alloys was found to decrease with Cr content from 8.32 g/cm3 for samples containing 0.5 wt%Cr to 7.26 g/cm3 for samples containing 12 wt%Cr. Therefore, the estimated porosity was found to increase from 6.4% to 16.6% with increasing Cr content from 0.5 wt% to 12 wt%. The microhardness of the samples varied from 153.6 to 284.2 VHN for the equiaxed grains and from 119.6 to 240.6 VHN for the island areas.

The Hi-GAL catalogue of dusty filamentary structures in the Galactic plane

ABSTRACT The recent data collected by Herschel have confirmed that interstellar structures with a filamentary shape are ubiquitously present in the Milky Way. Filaments are thought to be formed by several physical mechanisms acting from large Galactic scales down to subparsec fractions of molecular clouds, and they might represent a possible link between star formation and the large-scale structure of the Galaxy. In order to study this potential link, a statistically significant sample of filaments spread throughout the Galaxy is required. In this work, we present the first catalogue of 32 059 candidate filaments automatically identified in the Herschel Infrared Galactic plane Survey (Hi-GAL) of the entire Galactic plane. For these objects, we determined morphological (length la and geometrical shape) and physical (average column density $N_{\rm H_{2}}$ and average temperature T) properties. We identified filaments with a wide range of properties: 2 ≤ la ≤ 100 arcmin, $10^{20} \le N_{\rm H_{2}} \le 10^{23}$ cm−2 and 10 ≤ T ≤ 35 K. We discuss their association with the Hi-GAL compact sources, finding that the most tenuous (and stable) structures do not host any major condensation. We also assign a distance to ∼18 400 filaments, for which we determine mass, physical size, stability conditions and Galactic distribution. When compared with the spiral arms structure, we find no significant difference between the physical properties of on-arm and inter-arm filaments. We compare our sample with previous studies, finding that our Hi-GAL filament catalogue represents a significant extension in terms of Galactic coverage and sensitivity. This catalogue represents a unique and important tool for future studies devoted to understanding the filament life-cycle.

Electrical treeing in silicone gel under repetitive voltage impulses

Silicone gel is widely used to encapsulate power electronic modules. The weakness of the electrical insulation is surface discharges initiated at the gel-substrate-electrode triple junction and the subsequent formation of cavities; so called electrical trees. The propagation characteristics of cavities under high frequency and fast rise time voltages, which are typical waveforms formed in power electronic modules, have not been fully understood. Thus, in this research, the influence of frequency, rise time, and polarity on electrical treeing under repetitive voltage impulses are investigated. The results show that discharge tracks under positive impulses have filamentary shapes, and discharge tracks under negative impulses have spindle-shapes. The cavity length increases with the frequency and is especially long when the rise time is short under high frequency. This indicates the importance of understanding the cavity propagation characteristics under actual voltage waveforms in power electronics modules.

Direct Ink Writing of Cement Structures Modified with Nanoscale Additive

Synthetic structures built with cement benefit from high compressive strength, but their brittleness limits their fracture toughness under conditions where repeated, unnegotiable strains are imparted. This could be somewhat alleviated, if complex structures with tunable geometries is created, for example, via direct ink writing (DIW)‐based 3D printing. However, the nature of the slurries used in the DIW printing of cement must be modified with proper rheology to be effectively and programmatically printed with distinct mechanical properties intended for specific applications. Here, the authors have developed a nano‐clay modified cement‐based direct ink that enables high‐resolution 3D printing of complex architected structures of tunable geometries. The developed ink has a significant shear thinning and rapid gel strength properties which facilitate extrusion from a micro‐nozzle (≈400 μm) under ambient conditions conserving the filamentary shape with holding the load of the subsequent printed layer above. A series of architected structures have revealed how nanoscale additive, fabrication process, and architecture of the structures can influence both the stiffness and toughness in the cementitious materials. Understanding these construction principles based on architectures, materials, and processing can change the brittle cement‐based structure to a tough one for structural and functional applications.

The discovery of an asymmetric detached shell around the “fresh” carbon AGB star TX Psc

AbstractWe present ALMA observations of the circumstellar envelope around the AGB carbon star TX Psc in molecular CO(2–1) emission, and detect a previously unknown detached shell with filamentary structure and elliptical shape. Up to now, all observed detached shells are found around carbon AGB stars and are of remarkable spherical symmetry. The elliptical shell around TX Psc is the first clear exception to that rule, with TX Psc being classified as rather ’’fresh’’ carbon star, that most likely has only experienced very few thermal pulses yet. We investigate and discuss the 3D structure of the CSE and its most likely formation scenarios, as well as the link of this peculiar detached shell to the AGB evolutionary status of TX Psc.

ALMA Images of the Host Cloud of the Intermediate-mass Black Hole Candidate CO−0.40–0.22*: No Evidence for Cloud–Black Hole Interaction, but Evidence for a Cloud–Cloud Collision

Abstract This paper reports a reanalysis of archival ALMA data of the high velocity(-width) compact cloud CO−0.40–0.22, which has recently been hypothesized to host an intermediate-mass black hole (IMBH). If beam-smearing effects, difference in beam sizes among frequency bands, and Doppler shift due to the motion of the Earth are considered accurately, none of the features reported as evidence for an IMBH in previous studies are confirmed in the reanalyzed ALMA images. Instead, through analysis of the position–velocity structure of the HCN J = 3–2 data cube, we have found kinematics typical of a cloud–cloud collision (CCC), namely, two distinct velocity components bridged by broad emission features with elevated temperatures and/or densities. One velocity component has a straight filamentary shape with approximately constant centroid velocities along its length but with a steep, V-shaped velocity gradient across its width. This contradicts the IMBH scenario but is consistent with a collision between two dissimilar-sized clouds. From a non-LTE analysis of the multitransition methanol lines, the volume density of the post-shock gas has been measured to be ≳106 cm−3, indicating that the CCC shock can compress gas in a short timescale to densities typical of star-forming regions. Evidence for star formation has not been found, possibly because the cloud is in an early phase of CCC-triggered star formation or because the collision is nonproductive.

The evolution of giant molecular filaments

In recent years there has been a growing interest in studying giant molecular filaments (GMFs), which are extremely elongated (> 100pc in length) giant molecular clouds (GMCs). They are often seen as inter-arm features in external spiral galaxies, but have been tentatively associated with spiral arms when viewed in the Milky Way. In this paper, we study the time evolution of GMFs in a high-resolution section of a spiral galaxy simulation, and their link with spiral arm GMCs and star formation, over a period of 11Myrs. The GMFs generally survive the inter-arm passage, although they are subject to a number of processes (e.g. star formation, stellar feedback and differential rotation) which can break the giant filamentary structure into smaller sections. The GMFs are not gravitationally bound clouds as a whole, but are, to some extent, confined by external pressure. Once they reach the spiral arms, the GMFs tend to evolve into more substructured spiral arm GMCs, suggesting that GMFs may be precursors to arm GMCs. Here, they become incorporated into the more complex and almost continuum molecular medium that makes up the gaseous spiral arm. Instead of retaining a clear filamentary shape, their shapes are distorted both by their climb up the spiral potential and their interaction with the gas within the spiral arm. The GMFs do tend to become aligned with the spiral arms just before they enter them (when they reach the minimum of the spiral potential), which could account for the observations of GMFs in the Milky Way.

The Fabric of the Universe: Exploring the Cosmic Web in 3D Prints and Woven Textiles

We introduce The Fabric of the Universe, an art and science collaboration focused on exploring the cosmic web of dark matter with unconventional techniques and materials. We discuss two of our projects in detail. First, we describe a pipeline for translating three-dimensional (3D) density structures from N-body simulations into solid surfaces suitable for 3D printing, and present prints of a cosmological volume and of the infall region around a massive cluster halo. In these models, we discover wall-like features that are invisible in two-dimensional projections. Going beyond the sheer visualization of simulation data, we undertake an exploration of the cosmic web as a three-dimensional woven textile. To this end, we develop experimental 3D weaving techniques to create sphere-like and filamentary shapes and radically simplify a region of the cosmic web into a set of filaments and halos. We translate the resulting tree structure into a series of commands that can be executed by a digital weaving machine, and present a large-scale textile installation.

Addressing the electronic properties of III–V nanowires by photoluminescence excitation spectroscopy**This article belongs to the special issue: Emerging Leaders, which features invited work from the best early-career researchers working within the scope of J. Phys. D. This project is part of the Journal of Physics’ series 50th anniversary celebrations in 2017. Marta De Luca was selected by the Editorial Board

Semiconductor nanowires (NWs) have been attracting an increasing interest in the scientific community. This is due to their peculiar filamentary shape and nanoscale diameter, which renders them versatile and cost-effective components of novel technological devices and also makes them an ideal platform for the investigation of a variety of fascinating physical effects. Absorption spectroscopy is a powerful and non-destructive technique able to provide information on the physical properties of the NWs. However, standard absorption spectroscopy is hard to perform in NWs, because of their small volume and the presence of opaque substrates. Here, we demonstrate that absorption can be successfully replaced by photoluminescence excitation (PLE). First, the use of polarization-resolved PLE to address the complex and highly-debated electronic band structure of wurtzite GaAs and InP NWs is shown. Then, PLE is used as a statistically-relevant method to localize the presence of separate wurtzite and zincblende NWs in the same InP sample. Finally, a variety of resonant exotic effects in the density of states of InxGa1−xAs/GaAs core/shell NWs are highlighted by high-resolution PLE.

Applicability of FT-IR Techniques and Goniometry on Characterization of Carbon Fiber Surfaces

Carbon fibers have been widely used as structural reinforcement in aeronautical composites, because of their exceptional mechanical properties. However, carbon fibers present few polar groups on their surfaces inducing a weak interaction with some thermosetting and thermoplastic matrices. Surface treatments, such as thermal and electrochemical oxidation, are generally followed by sizing in order to improve interfacial adhesion between carbon fibers and some matrices. The precise surface characterization, by means of its chemical groups and radical identification, has been pointed out as an effective tool of carbon fibers finishing assessment process. Goniometry and Fourier transform- infrared spectroscopy are widely used to characterize some materials applied in industry, but not for carbon fibers, mainly because of their filamentary shape and the high concentration of carbon even in their near surface. In this paper, unsized and sized Torayca T300 carbon fibers were characterized by goniometry tests to evaluate hydrophilic or hydrophobic character. Qualitatively, it was noticed the effect of sizing on carbon fibers by their hydrophilic behavior, which has not been observed in unsized samples. Chemical analysis was performed by Fourier transform-infrared spectroscopy using different setups: photoacoustic, attenuated total reflectance and universal attenuated total reflectance. The Fourier transform-infrared spectroscopy/attenuated total reflectance/Germanium was shown to be the most promising technique to analyze carbon fibers surface, despite the low level of relative intensities of some bands. By this technique, it was possible to observe differences between unsized and sized carbon fibers spectra.

Engineering Escherichia coli for enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in larger cellular space

Polyhydroxyalkanoates (PHA) are intracellularly accumulated as inclusion bodies. Due to the limitation of the cell size, PHA accumulation is also limited. To solve this problem, Escherichia coli was enlarged by over-expression of sulA gene to inhibit the cell division FtsZ ring assembly, leading to the formation of filamentary E. coli that have larger internal space for PHA accumulation compared with rod shape E. coli. As a result, more than 100% increases on poly(3-hydroxybutyrate) (PHB) contents and cell dry weights (CDW) were achieved compared with its control strain under same conditions. The enlarged cell strategy was applied to the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) or P(3HB-co-4HB) by sad, gabD, essential genes ispH and folK knockout E. coli harboring two addictives and thus stable plasmids consisting of P(3HB-co-4HB) producing genes, including phaCAB operon, orfZ, 4hbD, sucD, essential genes ispH and folK as well as the sulA. The so constructed E. coli grew in glucose to form filamentary shapes with an improved P(3HB-co-4HB) accumulation around 10% more than its control strain without addition of 4HB precursor, reaching over 78% P(3HB-co-4HB) in CDW. Importantly, the shape changing E. coli was able to precipitate after 20min stillstand. Finally, the filamentary recombinant E. coli was not only able to produce more P(3HB-co-4HB) from glucose but also allow convenient downstream separation from the fermentation broth.