Filament Breakage Research Articles

Cable bacteria colonise new sediment environments through water column dispersal.

Cable bacteria exhibit a unique metabolism involving long-distance electron transport, significantly impacting elemental cycling in various sediments. These long filamentous bacteria are distributed circumglobally, suggesting an effective mode of dispersal. However, oxygen strongly inhibits their activity, posing a challenge to their dispersal through the water column. We investigated the effective dispersal of marine cable bacteria in a compartmentalised microcosm experiment. Cable bacteria were grown in natural 'source' sediment, and their metabolic activity was recorded in autoclaved 'destination' cores, which were only accessible through oxygenated seawater. Colonisation occurred over weeks, and destination cores contained only one cable bacterium strain. Filament 'snippets' (fragments with a median size of ~15 cells) accumulated in the microcosm water, with about 30% of snippets attached to sediment particles. Snippet release was also observed in situ in a salt marsh creek. This provides a model for the dispersal of cable bacteria through oxygenated water: snippets are formed by filament breakage in the sediment, released into the overlying water and transported with sediment particles that likely offer protection. These insights are informative for broader theories on microbial community assembly and prokaryotic biogeography in marine sediments.

Externally supplied ascorbic acid moderates detrimental effects of UV-C exposure in cyanobacteria.

The defensive role performed by exogenously supplied ascorbic acid in the cyanobacterium Nostoc muscorum Meg1 against damages produced by UV-C radiation exposure was assessed in this study. Exposure to UV-C (24mJ/cm2) significantly enhanced reactive oxygen species (ROS) (50%) along with peroxidation of lipids (21%) and protein oxidation (22%) in the organism. But, addition of 0.5mM ascorbic acid prior to UV-C exposure showed reduction in ROS production (1.7%) and damages to lipids and proteins (1.5 and 2%, respectively). Light and transmission electron microscopic studies revealed that ascorbic acid not only protected filament breakage but also restricted severe ultrastructural changes and cellular damages in the organism. Although the growth of the organism was repressed up to 9% under UV-C treatment within 15days, a pre-treatment with ascorbic acid led to growth enhancement by 42% in the same period. Various growth parameters such as photo-absorbing pigments (phycoerythrin, phycocyanin, allophycocyanin, chlorophyll a, and carotenoids), water splitting complex (WSC), D1 protein, RuBisCO, glutamine synthetase and nitrogenase activities in the UV-C treated organism were seen to be relatively intact in the presence of ascorbic acid. Thus, a detailed analysis undertaken in the present study was able to demonstrate that ascorbic acid not only act as first responder against harmful UV-C radiation by down-regulating ROS production, it also accelerated the growth performance in the organism in the post UV-C incubation period as an immediate response to an adverse experience presented in the form of UV-C radiation exposure.

Nanogap resistive switch mechanism study and performance degradation analysis

The nanogap resistive switch holds potential as a candidate for nonvolatile memory, although its durability needs enhancement. This study delves into the operational mechanisms through detailed morphological examination during continuous operation of nanogap resistive switches. By developing a finite element model of nanogaps, we reveal the mechanisms behind the formation of electrode surface hillocks and filaments during continuous switching. Our findings suggest that “set” operations include processes such as field evaporation, electric field-induced diffusion, and field-assisted migration within the gap. Conversely, “reset” operations, driven by Joule heating and electromigration, lead to filament breakage and the creation of a fine gap. This research elucidates device degradation issues, such as periodic fluctuations in set threshold voltage (Vset) and the presence of non-steep set curves, providing both theoretical and experimental insights to improve future device performance.

Numerical simulation of droplet characterized by Rolie–Poly model with finite extensibility passing through cylinder obstacles

The deformation and rupture of viscoelastic droplet passing through cylinder obstacles in a microchannel are investigated using OpenFOAM. The constitute relationship of droplet is modeled by the Rolie–Poly model with finite extensibility, and the two-phase interface is tracked by the volume of fluid method. The effects of capillary number (Ca), the distance between cylinders (l1), relaxation time ratio (ξ), Weissenberg number (Wi), etc., on droplet deformation and rupture are mainly explored. When Ca decreases, the symmetry of droplet rupture changes and three behaviors of the droplet, i.e., symmetrical rupture, asymmetrical rupture, and non-rupture, can be captured. Further research shows that the stagnation area formed between cylinders is broken with the increase in l1, where the two sub-droplets merge again. Viscoelastic droplet with a smaller relaxation time ratio ξ is more likely to extend into thin and durable filament. Especially, when ξ=0.2, the filament will break many times during the stretching process. During above-mentioned two kinds of development, the normal stress difference develops obviously at the places, where the filament breaks or the sub-droplets combine together. This may imply that the normal stress difference facilitates the rupture and coalescence of droplets. In addition, with the increase in elasticity, the normal stress difference tends to develop at the phase interface.

Spatially Resolved Defect Characterization and Fidelity Assessment for Complex and Arbitrary Irregular 3D Printing Based on 3D P-OCT and GCode.

To address the challenges associated with achieving high-fidelity printing of complex 3D bionic models, this paper proposes a method for spatially resolved defect characterization and fidelity assessment. This approach is based on 3D printer-associated optical coherence tomography (3D P-OCT) and GCode information. This method generates a defect characterization map by comparing and analyzing the target model map from GCode information and the reconstructed model map from 3D P-OCT. The defect characterization map enables the detection of defects such as material accumulation, filament breakage and under-extrusion within the print path, as well as stringing outside the print path. The defect characterization map is also used for defect visualization, fidelity assessment and filament breakage repair during secondary printing. Finally, the proposed method is validated on different bionic models, printing paths and materials. The fidelity of the multilayer HAP scaffold with gradient spacing increased from 0.8398 to 0.9048 after the repair of filament breakage defects. At the same time, the over-extrusion defects on the nostril and along the high-curvature contours of the nose model were effectively detected. In addition, the finite element analysis results verified that the 60-degree filling model is superior to the 90-degree filling model in terms of mechanical strength, which is consistent with the defect detection results. The results confirm that the proposed method based on 3D P-OCT and GCode can achieve spatially resolved defect characterization and fidelity assessment in situ, facilitating defect visualization and filament breakage repair. Ultimately, this enables high-fidelity printing, encompassing both shape and function.

Act88F pseudo-acetylation of lysine 50 (K50) negatively regulates muscle function in Drosophila

Non-histone post-translational modifications (PTMs) are an increasingly important area of study that contribute to our understanding for protein function in the control of health and disease. Protein PTMs, such as acetylation, are emerging as key regulators of striated muscle function, yet our understanding of how non-histone protein acetylation affects muscle physiology remains fragmentary. Previous reports from our lab identified lysine (K) 52 of skeletal muscle alpha actin (ACTA1) to be acetylated, yet no reports have shown a role for ACTA1 acetylation in muscle function. Act88F is 93% homologous to ACTA1 and K50 is identical to K52 of ACTA1. Thus, for these studies, we mutated K50 with a glutamine (Q) on the Act88F gene in the indirect flight muscle (IFM) of Drosophila to mimic acetylation. We examined physiological function (i.e. flight and climbing) as well as biophysical changes between acetylated Act88F and the associated myofibrillar proteins involved in contraction and relaxation (myosin, tropomyosin, and troponin). We report that Act88F K50Q pseudo-acetylation resulted in a flightless phenotype and decreased climbing ability. In addition, we report that Act88F K50Q flies had increased IFM damage as examined via fluorescent imaging. Interestingly, Act88F K50Q did not change actin-myosin binding, actin sliding velocity, or Ca2+ sensitivity in actin-motility assays. However, Act88F pseudo-acetylation did decrease actin filament length, but only after interacting with myosin, implying that Act88F acetylation increases actin filament breaking. These data suggest that Act88F acetylation at K50 worsens muscle function by destabilizing filamentous actin, resulting in muscle tearing and damage to the IFM, which results in loss of flight. This work suggests that actin acetylation, at least on lysine 50, in response to environmental stressors or diet could greatly change muscle function and sarcomere integrity. This research was funded by the Dennis Meiss & Janet Ralston Fund for Nutri-epigenetic Research, the National Institute for General Medical Sciences (NIGMS) of the NIH (P20 GM130459), the National Heart, Lung, and Blood Institute of the NIH (R15 HL143496), National Institute of Aging of the NIH (R21 AG077248) and NSF EPSCOR Track II (OIA-1826801) to B.S.F. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Mapping 3D printed part density and filament flow characteristics in the material extrusion (MEX) process for filled and unfilled polymers

ABSTRACT Material extrusion (MEX) 3D printing offers a promising avenue for fabricating metal and ceramic components, where highly loaded polymer filaments are 3D-printed and sintered. Achieving nearly 100% 3D-printed part density is critical in this process, as porosity during printing compromises part properties after sintering. However, challenges arise due to the unfavourable mechanical and rheological properties of MEX metal filaments, leading to slow print-speeds, filament breakage, and inconsistent extrusion. This work explores these process-property correlations by developing process maps for bound metal filaments and unfilled polymer systems. This study throws light on the significance of back-flow and back-pressure in calculating the pressure drop across the nozzle, especially in a diverse set of material systems represented by hard and stiff PLA, soft and flexible TPU, and brittle and fragile bronze filaments. Additionally, ANOVA identified the effect of print conditions on measured variables, while regression models helped predict the behaviour of a given material under different process conditions. Therefore, this study enables material design and discovery for bound-metal filaments while addressing critical knowledge gaps, thereby paving the way for high-density 3D-printed components.

AutoML-driven diagnostics of the feeder motor in fused filament fabrication machines from direct current signals

AbstractPart defects in additive manufacturing are more frequent compared to machining or molding. Failures can go unnoticed for hours, wasting resources and extending process cycle times. This paper describes a Machine Learning based method for automated sensing of onset failure in additive manufacturing machinery. Investigations are conducted on a Fused Filament Fabrication (FFF) 3D printer, and the same methods are then applied to a digital light processing 3D printer. The investigation focuses on signal-based analysis, specifically passive sensing of stepper motors relating DC current measurements to the torque on a stepper, as opposed to any active acoustic interrogation of the part. Passive methods are used to characterize the loading on a feeder stepper in an FFF machine, forming a model that can identify early signs of filament-based failure with 85.65% 10-fold cross-validation accuracy. Efforts show filament breakage can be detected minutes before material runout would cause a defect, allowing ample time to pause, correct, or control the print. The machine learning pipeline was not naively conceived but optimized through automated machine learning.

Orthotropic mechanical properties of PLA materials fabricated by fused deposition modeling

Polylactic Acid (PLA) sample manufactured by fused deposition modeling (FDM) technique is typically assumed to be transversely isotropic without a thorough examination of its orthotropy in three-dimensional space. This study investigated the mechanical orthotropy of PLA samples with two air gap levels (0 mm and -0.05 mm) and various loading directions. Tensile strength and elastic modulus were experimentally measured and the Hill48 yield model and orthotropic elastic model were calibrated. Results revealed that the anisotropy induced by interlayer bond was more pronounced than that within layer. Introducing a -0.05 mm air gap remarkably reduced voids in printed samples, enhancing the stiffness and strength of tensile samples. It also delayed the transition of fracture modes in intra-layer samples as the filament angle increased from 0° to 90°, which shifted from filament breakage to combined fracture mode and subsequently to interface failure. Despite these improvements, the inherent anisotropy of FDM printed PLA materials remained due to the oriented molecular chains and insufficient chain diffusion. The study emphasizes the importance of orthotropic mechanical models, demonstrating their reliability through calibration with acceptable accuracy.

Effect of Ni-doped on switching mechanisms and characteristics of ZnO-based memristor: Experimental and first-principles investigations

In this paper, we explore the impact of Ni doping on the resistive switching (RS) performance of ZnO-based resistive random access memory (RRAM). The Ti/ZnO: Ni/ITO resistive memory exhibits a reduced and less discrete forming voltage (Vforming) and set voltage (Vset), along with a more stable high resistance state (HRS). The doping does not change the conductivity mechanism of the device. The observed outcomes can be attributed to the influence of Ni doping on the generation and breakage of the oxygen vacancy conductive filaments (CF) in the vicinity. Using First-Principles calculations, we calculated the formation energy of oxygen vacancies (Vos), the band structure, the density of states (DOS), charge density, and electronic properties of the doped ZnO cell. The oxygen vacancy formation energy (Efv) is significantly lower in the Ni-doped ZnO cell, which is associated with lower Vforming and Vset. And it allows for less randomness in the generation of CF, which improves the stability of the HRS. The band structure shows that Ni doping can add an additional band at the Fermi energy level of ZnO, corresponding to the defect states in the density of states. The improved storage capacity of Ni-doped ZnO devices has been experimentally demonstrated.

Investigation on 3D printing of shrimp surimi under different printing parameters and thermal processing conditions

Improving the printing accuracy and stability of shrimp surimi and finding appropriate printing parameters and suitable thermal processing method can help to develop high value-added 3D printing products of shrimp surimi. It was found that in order to make the 3D printing products of shrimp surimi have higher printing adaptability (printing accuracy and printing stability reach more than 97%), by choosing nozzle diameter of 1.20 mm and setting the printing height of the nozzle to 2.00 mm, the layers of the printed products were better fused with each other, and the printing accuracy of the products could be greatly improved; there was no uneven discharge and filament breakage when the nozzle moved at the speed of 30 mm/s; and the products were internally compact and had good stability when the printing filling rate was 80%. In addition, the deformation rates of steamed, boiled and deep-fried shrimp surimi products were significantly higher than those of oven-baked and microwaved shrimp surimi products (P < 0.05). Microwave heating had a greater effect on the deformation and color of shrimp surimi products, and was not favored by the evaluators. In terms of deformation rate, sensory score, and textural characteristic, the oven-baked thermal processing method was selected to obtain higher sensory evaluation scores and lower deformation rates of shrimp surimi 3D printed products. In the future, DIY design can be carried out in 3D printing products of shrimp surimi to meet the needs of different groups of people for modern food.

Properties of multi-walled carbon nanotubes grown three-dimensional (3D) woven carbon/epoxy multiscale composites: Experimental study

Multiwalled carbon nanotubes (MWCNTs) were grown onto the surface of woven carbon fiber fabrics (CFFs) by using a chemical vapor deposition method. The mechanical and electrical properties of MWCNTs grown three-dimensional (3D) woven carbon/epoxy multiscale composite (CP-gCNT-E) structures were experimentally investigated. The warp directional flexural and interlaminar shear strength of CP-gCNT-E was slightly decreased by 18.26% and 28.32%, respectively compared to the pristine sample. However, the CP-gCNT-E composite at 20 °C showed 595.82% (7.0 folds) higher electrical conductivity than the pristine. On the other hand, delamination was suppressed via three dimensional vertically aligned three dimensional networks of grown carbon nanotubes (gCNTs) in the through-the-thickness direction of multiscale composite with various failure mechanism including entangled gCNT straightening, CNT bridging, pull-out, and stick-slip along with CNTs, carbon filament and matrix breakages which caused a stress dampening and slowing down the crack propagation. In addition, this network topology in the structure enhanced its electrical conductivity. Therefore, the CP-gCNT-E composite was considered as “damage tolerant” material.

Mechanical and dynamic mechanical behavior of 3D printed waste slate particles filled acrylonitrile butadiene styrene composites

This study analyses the feasibility of utilizing waste slate particles as a strengthening component in affordable 3D-printed composite materials to address environmental issues associated with waste management. Composite filaments were created by incorporating waste slate particles (5 %, 10 %, and 15 % by weight) into an acrylonitrile butadiene styrene (ABS) matrix and subsequently evaluated for mechanical properties. A twin-screw extruder obtained the composite filaments, while test samples were produced using a 3D printer by fused deposition modelling. The developed composites have a gradual variation in evaluated properties, about an 8 % increment in hardness, and a 40 % increment in flexural and tensile modulus was noted on the inclusion of 15 wt% of waste slate particles in the ABS matrix. The composites filled with 10 wt% and 5 wt% waste slate particles exhibited the maximum flexural strength (65.24 MPa) and tensile strength (42.11 MPa), respectively. These values were 5 % and 8 % higher than those of unfilled ABS. Furthermore, it was shown that the ABS matrix saw a decrease in elongation and impact strength as the dosage of slate particles increased. The fractured surface morphology indicated that filament breaking, filler pullout, and the creation of voids between neighbouring layers were the primary causes of material failure. The dynamic mechanical analysis (DMA) results show the reinforcing effect of slate particles by an increase of storage modulus with 13 % at 35 °C for the composite with 10 wt% slate particles and 20 % for that with 15 wt% slate particles. DMA results analyzed the composite's adhesion factor, degree of entanglement, reinforcement efficiency factor, and effectiveness factor to clarify the reinforcing mechanism of slate particles. The study determines that the most effective utilization of waste slate particles in an ABS matrix is achieved by including 10 wt%. The results of this study enhance the concept of recycling waste slate powder and reveal the potential for their utilization in several industries, including the aerospace, automotive, healthcare, and architectural sectors of 3D printing.

Improving water removal efficiency in a PEM fuel cell: Microstructured surfaces for controlling instability-driven pinching

Proton Exchange Membrane Fuel Cell (PEMFC) is a clean, sustainable energy generation device, and its large-scale usage is becoming popular due to green and secure energy demand worldwide. The performance, efficiency, and lifespan of PEMFC largely depend on the water removal and management within the cell. Under the influence of the cross-air flow, the generated water filaments deform, and as the filament radius lowers, the curvature and capillary pressure increase, ejecting fluid out of the neck at increasing velocities. The moment the filament radius vanishes, the governing equations reach the point of singularity, and the filament breaks. We propose an optimum micro-patterned surface design for efficient water removal from PEMFC. We perform a numerical study of water generation on the surface followed by breakup under shear flow within confinement. We further theoretically identify the breakup behavior with characterization, recognizing the influence of the microstructures toward an efficient design. The hydrophobic microstructures are observed to decrease the dominance of viscous force over inertia and capillary force. This leads to a greater propensity of end-pinching or truncation of the generated droplet at the neck, which reduces the production of undesired satellite droplets that would have otherwise caused flooding of the chamber. In this work, we show that a proper combination of substrate structure and jet velocity-induced shear can mitigate the generation of satellite droplets and reduce the breakup time, significantly increasing the water removal efficiency of the PEMFC.

Nonvolatile and volatile resistive switching characteristics in MoS2 thin film for RRAM application

Resistive random access memory (RRAM) is one of strong candidates for future memory technology. The volatile and nonvolatile properties of devices are important foundations for the construction of neuromorphic hardware systems, which still represents a challenge. In this study, we fabricated the Cu/MoS2/ITO RRAM by RF magnetic sputtering with different thicknesses of MoS2 thin films, and explored the possibility of obtaining the nonvolatile and volatile memristive effects of RRAM devices through different operating voltages. By systematically investigating the resistive switching (RS) characteristics, we proposed and discussed the nonvolatile and volatile conceptual models to elaborate on the RS mechanism of the fabricated devices. The nonvolatile and volatile behaviors were explained respectively by the forming and breaking of Cu conductive filaments between Cu and ITO, and the trapping and de-trapping of electrons leading to the change of the Schottky barrier at the ITO/MoS2 interface.

Impedance Analysis of Three-Dimensional Continuous Carbon Filament Embroidered Electrodes in Large 300 cm2 Redox Flow Cells

The paper investigates the use of three-dimensional (3D) continuous carbon filament electrodes prepared using tailored fiber placement (i.e. embroidered electrodes) in a 300 cm2 redox flow cell with 50% state-of-charge (SOC) ferro/ferricyanide redox couple as the probe electrolyte. Electrochemical impedance spectroscopy (EIS) was conducted to identify the different resistance contributions, and thus voltage losses of the electrodes. The findings indicate that: (1) achievingh high frequency resistance values comparable to the felts is possible through side contacting of continuous filament electrodes to the graphite plates, eliminating the need to press the entire electrode structure. (2) The embroidered electrodes can minimize pressure drop, regardless of the electrode thickness, due to the parallel orientation of the carbon filaments to the electrolyte flow, resulting in reduced hydraulic resistance. (3) To reduce charge-transfer resistances, an oxidation treatment is required to improve the wettability of the electrodes, and the duration of the activation treatment must be optimized to avoid filament breakage due to etching. (4) Embroidered electrodes exhibit higher mass transfer coefficients thanfelts, which is attributed to the perpendicular orientation of the carbon filaments to the electrolyte flow. The paper provides avenues for further development of 3D carbon fiber electrodes.

Nanoscopic jets and filaments of superfluid 4He at zero temperature: A DFT study.

The instability of a cryogenic 4He jet exiting through a small nozzle into vacuum leads to the formation of 4He drops, which are considered ideal matrices for spectroscopic studies of embedded atoms and molecules. Here, we present a He-density functional theory (DFT) description of droplet formation resulting from jet breaking and contraction of superfluid 4He filaments. Whereas the fragmentation of long jets closely follows the predictions of linear theory for inviscid fluids, leading to droplet trains interspersed with smaller satellite droplets, the contraction of filaments with an aspect ratio larger than a threshold value leads to the nucleation of vortex rings, which hinder their breakup into droplets.

Effects of Quantum Recoil Forces in Resistive Switching in Memristors

Memristive devices, whose resistance can be controlled by applying a voltage and further retained, are attractive as possible circuit elements for neuromorphic computing. This new type of devices poses a number of both technological and theoretical challenges. Even the physics of the key process of resistive switching, usually associated with formation or breakage of conductive filaments in the memristor, is not completely understood yet. This work proposes a new resistive switching mechanism, which should be important in the thin-filament regime and take place due to the back reaction, or recoil, of quantum charge carriers – independently of the conventional electrostatically-driven ion migration. Since thinnest conductive filaments are in question, which are only several atoms thick and allow for a quasi-ballistic, quantized conductance, we use a mean-field theory and the framework of nonequilibrium Green’s functions to discuss the electron recoil effect for a quantum current through a nanofilament on its geometry and compare it with the transmission probability of charge carriers. Namely, we first study an analytically tractable toy model of a 1D atomic chain, to qualitatively demonstrate the importance of the charge-carrier recoil, and further proceed with a realistic molecular-dynamics simulation of the recoil-driven ion migration along a copper filament and the resulting resistive switching. The results obtained are expected to add to the understanding of resistive switching mechanisms at the nanoscale and to help downscale high-retention memristive devices.

Fragmentation and Entanglement Limit Vimentin Intermediate Filament Assembly

Experiments and theoretical modeling show that disassembly of vimentin intermediate filaments---a key process in many biological cell functions---proceeds via filament breakage without the assistance of other proteins.

Instant yield stress measurement from falling drop size: The “syringe test”

We analyze different flow regimes of a filament formed by extrusion of a material through a cylindrical die. We deduce that the elongational yield stress of a simple yield stress fluid (i.e., with negligible thixotropy effects) can be determined from the mass of the droplet after filament breakage and an estimation of the critical radius at pinch-off at the solid-liquid regime transition. We demonstrate that such a simple characterization is relevant in a relatively wide range of extrusion velocities, i.e., this velocity slightly affects the drop mass in this range. For the simple yield stress fluids used, Carbopol gel, clay-water paste at different concentrations, and emulsion, covering a large range of yield stress values (50–1000 Pa), the elongational yield stress appears to be equal to the simple shear yield stress times a factor equal to about 1.53. As a consequence, this simple test may be used to obtain, almost instantaneously and without sophisticated apparatus (a syringe and a balance are sufficient), a good estimate of the shear yield stress of simple yield stress fluids. In that case, the main source of uncertainty (up to about 20%) is the value of the critical radius at the solid-liquid transition. Finally, we review the operating conditions (material properties and extrusion characteristics) for which we can expect this approach to be valid.