Field Resistance Research Articles

The influences of alloying elements and processing conditions on the machinability of wrought aluminium alloys: A literature review

Today, wrought aluminium alloys are ranked among the most important materials with low specific density, strength features, corrosion resistance, machinability and recyclability properties in numerous application fields. Machining process of these alloys involves a number of considerations to ensure the highest quality and productivity. Key machinability issues include the rate of tool wear, workpiece surface finish and integrity and machining process sustainability. Understanding and optimising these parameters can lead to improving the machinability, better surface finish, longer tool life, and overall, more efficient and cost-effective machining processes for wrought aluminium alloys. The machining of wrought aluminium alloys remains a challenging task due to their unique characteristics, such as high strength, thermal conductivity and refined grain structures with mechanical and/or heat treatment. Although considerable progress has been made in the development of cutting tool materials and machining techniques for wrought aluminium alloys, further research is required to improve the machinability of these materials and reduce the associated costs and time needed for production. Having access to relevant information about these characteristics can help industries and researchers make informed decisions and achieve better outcomes when machining of these important materials. Accordingly, in the current research work, a comprehensive study has been carried out on machining of wrought aluminium from different points of view.

Research on humidity field and freezing resistance of green recycled ceramic internal cured concrete at early age

In cold and drought environment, concrete shrinkage cracking caused by water loss is serious, and thus the freezing resistance of concrete is deteriorated. The waste ceramic particles were used as internal curing materials to prepare concrete, based on this background. Then, the influence of internal curing with ceramic particles on humidity field and temperature of concrete is analyzed by using temperature and humidity sensor. Based on the change mechanism of the internal humidity of concrete, the relationship between the curvature radius of liquid surface and the relative humidity is revealed. Taking water loss and water loss rate as the index, the water transfer law of concrete surface under ceramic particle internal curing was defined. Through the drying shrinkage test, the mechanism of improving the drying shrinkage of concrete by internal curing in ceramic particles was mastered. Finally, the effects of ceramic internal curing on cement hydration, interfacial transition zone (ITZ) and freezing resistance of concrete were studied. The results show that the relative humidity, water loss and water loss rate of concrete increase with the increase of ceramic particle content, and the dry shrinkage value decreases with the increase of ceramic particle content. Compared with ordinary concrete, when the ceramic particle content is 10%, 15%, 20%, 25% and 30%, and the test period is 10 days, the internal relative humidity of concrete increases by 0.52%, 0.67%, 1.55%, 2.55% and 3.05%, respectively. The drying shrinkage values decreased by 3.42%, 7.69%, 10.26%, 15.17% and 30.13%, respectively. When the test period was 1032 hours, the water loss increased by 1.08%, 5.15%, 7.90%, 12.69% and 14.49%, respectively. Furthermore, when the ceramic particle content is 20%, the initial normalized heat flow of concrete increases by 2.74 mW/g, and the normalized heat flow minimum increases by 0.324 mW/g. The minimum ITZ microhardness increased by 2.64 MPa, the ITZ width decreased by 5 μm, and the maximum crack width at the contact between ITZ and aggregate decreased by 0.89 μm. At the same time, when the ceramic particle content is less than 20%, the effect of internal curing on the freezing resistance of concrete increases with an increase in ceramic particle content.

Effect of settling vortex of coal slime flocs with different sizes on the settlement of microfine particles

This article explores the flow field changes and micro vortex formation mechanisms caused by coagulation and sedimentation of coal slurry flocs of different sizes. The collision adhesion rate, motion trajectory and force analysis of fine particles under micro vortex disturbance. Particle image velocimetry (PIV) and high-speed camera were used to capture deposition process of coal slime flocs, the changes of flow field and fine particles were analyzed by numerical simulation. The smaller flocs form a single vortex ane larger flocs show periodic vortex shedding, resulting in increase fluid velocity and larger interference area. The collision adhesion rate of fine particles in the 3.4 mm floc settling disturbance area is the largest, 9.45 %;Particles<0.3 mm are easily sucked into vortices, while particles>0.3 mm are disturbed by vortices and change their motion trajectory towards the vortex center. It is mainly affected by gravity, pressure gradient force, flow field resistance, and centrifugal force.

The V410L kdr allele in the VGSC confers higher levels of field resistance to permethrin in urban mosquito populations of Aedes aegypti (L.).

Females of Aedes aegypti transmit emerging arboviruses including Zika, dengue, yellow fever, and chikungunya. Control of these adult mosquitoes heavily relies on synthetic insecticides, including pyrethroids. However, insecticide resistance development in populations poses a significant challenge to vector control, particularly from knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC), the target of pyrethroids. This study investigated the field efficacy of Permanone, a pyrethroid-based insecticide, against Ae. aegypti by assessing the impact of three common kdr mutations (V410L, V1016I, F1534C) on mosquito survival under a real operational mosquito control scenario, by quantifying the pesticide delivered in the field. Field cage tests (FCTs) were conducted while conducting a realistic mosquito control application. Female mosquitoes from six operational areas from Harris County, TX, USA were exposed to Permanone delivered with a handheld sprayer. Permanone deposited near the cages was estimated from aluminum boats placed in the field during FCTs using gas chromatography-mass spectrometry (GC-MS). Mortality rates were recorded, and individual mosquitoes were genotyped for kdr mutations. A probit regression model was used to analyze the factors influencing mosquito survivorship. As the distance from the application source route increased, the amount of Permanone deposited decreased, resulting in higher survivorship frequency of Ae. aegypti females with the triple-resistant kdr genotype (LL/II/CC). The L allele at the 410-site significantly contributed to an increased resistance level when co-occurring with other kdr mutations. This study linked the survival probabilities of mosquitoes with different kdr genotypes, and the amount of pesticide they received in the field. Pesticide quantification, control efficacy results and genotyping allowed us to empirically determine the impact of genotypic resistance on vector control in the field. © 2024 Society of Chemical Industry.

Identification of Resistance to &amp;lt;i&amp;gt;Puccinia striiformisf&amp;lt;/i&amp;gt;. sp. &amp;lt;i&amp;gt;tritici&amp;lt;/i&amp;gt; (&amp;lt;i&amp;gt;Pst&amp;lt;/i&amp;gt;) in Some Commercial and Elite Bread Wheat Lines

Stripe rust is caused by &amp;lt;i&amp;gt;Puccinia striiformisf&amp;lt;/i&amp;gt;. sp. &amp;lt;i&amp;gt;tritici&amp;lt;/i&amp;gt; (&amp;lt;i&amp;gt;Pst&amp;lt;/i&amp;gt;) threatening wheat production in Ethiopia. The emerged virulent stripe rust races at one point of the world spread to the rest of wheat-producing countries by the wind as well as human travels and damaged popular resistant wheat cultivars thereby posing food insecurity. However, wheat cultivars succumb to new Pst race (s) soon after their release from research centers. This study aimed to determine stripe rust resistance in some Ethiopian commercial and elite bread wheat lines. In 2017, a total of 37 commercial and elite bread wheat lines were exposed to stripe rust in under the disease hot spot areas (Kulumsa and Meraro) Arsi zone Oromia regional state. In the second year (2018), 22 promising lines consisting of 16 commercial bread wheat and 6 elite’s lines were evaluated both at seedling and adult plant growth stages. The seedling test was conducted in the greenhouse at Kulumsa research center using three Pst races. In field evaluations, terminal severity (TRS), coefficient of infection (CI), area under disease progress curve (AUDPC), disease progress rate (DPR), and spike infection (SI) were considered. Of the 37 commercial and elite bread wheat lines, 19 (51.4%) exhibited lower or equal disease reaction compared to the resistant check (Enjoy) across locations and seasons. Eleven bread wheat lines showed both adult plant and seedling resistance. The 37 commercial wheat lines that showed field resistance was further exposed to three Pst races at the seedling stage and 11 exhibited seedling resistance to all races. This study has identified seedling and adult plant resistance in some commercial and elite bread wheat lines to the prevailing Pst races.

Characterization of the wheat-tetraploid Thinopyrum elongatum 7E(7D) substitution line with Fusarium head blight resistance

BackgroundFusarium head blight (FHB), a devastating disease of wheat production, is predominantly elicited by Fusarium graminearum (Fg). The tetraploid Thinopyrum elongatum is a tertiary gene resource of common wheat that possesses high affinity and displays high resistance traits against multiple biotic and abiotic stress. We aim to employ and utilize the novel FHB resistance resources from the wild germplasm of common wheat for breeding.ResultsDurum wheat-tetraploid Th. elongatum amphiploid 8801 was hybridized with common wheat cultivars SM482 and SM51, and the F5 generation was generated. We conducted cytogenetically in situ hybridization (ISH) technologies to select and confirm a genetically stable 7E(7D) substitution line K17-1069-5, which showed FHB expansion resistance in both field and greenhouse infection experiments and displayed no significant disadvantage in agronomic traits compared to their common wheat parents in the field. The F2 segregation populations (K17-1069-5 × SM830) showed that the 7E chromosome conferred dominant FHB resistance with dosage effect. We developed 19 SSR molecular markers specific to chromosome 7E, which could be conducted for genetic mapping and large breeding populations marker-assisted selection (MAS) during selection procedures in the future. We isolated a novel Fhb7 allele from the tetraploid Th. elongatum chromosome 7E (Chr7E) using homology-based cloning, which was designated as TTE7E-Fhb7.ConclusionsIn summary, our study developed a novel wheat-tetraploid Thinopyrum elongatum 7E(7D) K17-1069-5 substitution line which contains stable FHB resistance.

Development of infectious clones of mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) infecting mungbean [Vigna radiata (L.) R. Wilczek] and evaluation of a RIL population for MYMIV resistance.

Yellow mosaic disease (YMD) is a major constraint for the low productivity of mungbean, mainly in South Asia. Addressing this issue requires a comprehensive approach, integrating field and challenge inoculation evaluations to identify effective solutions. In this study, an infectious clone of Begomovirus vignaradiataindiaense (MYMIV) was developed to obtain a pure culture of the virus and to confirm resistance in mungbean plants exhibiting resistance under natural field conditions. The infectivity and efficiency of three Agrobacterium tumefaciens strains (EHA105, LBA4404, and GV3101) were evaluated using the susceptible mungbean genotype PS16. Additionally, a recombinant inbred line (RIL) population comprising 175 lines derived from Pusa Baisakhi (MYMIV susceptible) and PMR-1 (MYMIV resistant) cross was developed and assessed for YMD response. Among the tested Agrobacterium tumefaciens strains, EHA105 exhibited the highest infectivity (84.7%), followed by LBA4404 (54.7%) and GV3101 (9.80%). Field resistance was evaluated using the coefficient of infection (CI) and area under disease progress curve (AUDPC), identifying seven RILs with consistent resistant reactions (CI≤9) and low AUDPC (≤190). Upon challenge inoculation, six RILs exhibited resistance, while RIL92 displayed a resistance response, with infection occurring in less than 10% of plants after 24 to 29 days post inoculation (dpi). Despite some plants remaining asymptomatic, MYMIV presence was confirmed through specific PCR amplification of the MYMIV coat protein (AV1) gene. Quantitative PCR revealed a very low relative viral load (0.1-5.1% relative fold change) in asymptomatic RILs and the MYMIV resistant parent (PMR1) compared to the susceptible parent (Pusa Baisakhi). These findings highlight the potential utility of the developed infectious clone and the identified MYMIV-resistant RILs in future mungbean breeding programs aimed at cultivating MYMIV-resistant varieties.

Resistance risk assessment of Rhizoctonia solani to four fungicides.

Hexaconazole, thifluzamide, difenoconazole and azoxystrobin are widely used fungicides for the control of Rhizoctonia solani in China. However, few studies have assessed the sensitivity and resistance risk of R. solani to these four fungicides. The sensitivities of 126 R. solani isolates to hexaconazole, thifluzamide, difenoconazole and azoxystrobin were determined, with average half maximal effective concentration (EC50) values of 0.0386, 0.0659, 0.663 and 1.508 μg mL-1, respectively. Field resistance monitoring of the four fungicides showed that the three isolates had moderate resistance to difenoconazole. Resistant mutants to the four fungicides were obtained by fungicide adaptation, and resistance could be stably inherited by most mutants. Compared with those of the parent isolates, the biological characteristics of hexaconazole-resistant mutants exhibited enhanced or similar compound fitness index (CFI), whereas most of the other mutants displayed reduced or comparable CFI. There was evidence of positive cross-resistance between hexaconazole and difenoconazole. In the presence of fungicides, the expression of the CYP51 genes in hexaconazole- and difenoconazole-resistant mutants significantly increased, the expression of SDH genes in thifluzamide-resistant mutants significantly decreased, and the expression of the Cyt b gene in azoxystrobin-resistant mutants did not significantly change. Based on these data, we speculated that R. solani had a low-to-medium resistance risk to four fungicides. The change of target gene expression may be one of the reasons for fungicide resistance in R. solani. This study provides a theoretical basis for monitoring resistance emergence and developing resistance management strategies to control R. solani. © 2024 Society of Chemical Industry.

Non-smooth discrete element method analysis of channel width's effect on ice resistance in broken ice field

A numerical model based on NDEM is used to investigate the effect of channel width on broken-ice resistance in the channel. The model incorporates a limited impulse method to simulate the ice crushing failure. The accuracy of the numerical model is benchmarked against experimental data from model tests conducted on the Araon icebreaker at KRISO. The simulations successfully capture the characteristic effects of channel width on broken-ice resistance observed in the experiment. Beyond benchmarking of the numerical model, this study analyses the force chain characteristics in the broken ice field, revealing the crucial role of force chains in the resistance increase caused by channel width constraints. Building upon the established numerical model, further sensitivity analyses are conducted to investigate the correlation between channel width effect and other relevant factors such as ice concentration, ship velocity and ice thickness. Among these factors, it is found that ice concentration emerges as the predominant determinant of the critical channel width. The increase in ice resistance attributable to the channel width is exacerbated under the condition of thicker ice floes and lower ship velocity, where the occurrence of ice floe rotation is less frequent and the corresponding force chains are stronger and more stable.

Graphene composite coating for enhanced corrosion resistance of Ni foam flow field in PEMFC

With the development of proton exchange membrane fuel cells (PEMFCs), metal foam with lightweight advantages and highly porous structures has become promising flow field material to replace traditional carbon plates. However, in the operating environment with high temperatures and acid situation, it suffers severe corrosion issues. To solve this problem, Sn and Sn/graphene composite coatings are prepared and the physical characteristics are analyzed by X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Tafel polarization test, potentiostat test, and electrochemical impedance spectroscopy are used to test the corrosion resistance of coated nickel foam in a PEMFC cathodic environment. Compared to Sn-coated foam, the corrosion resistance is further enhanced with the addition of graphene. The Sn/graphene coating obtained by electrodeposition at current density of 15mA·cm–2 has the lowest corrosion current density, which accounts for only 41.20% and 33.48% of the uncoated foam value at 50°C and 80°C, respectively. It also exhibits the maximum coating resistance, the lowest potentiostatic current and interfacial contact resistance, which is 66.3% lower than the US DOE 2025 goal. After an 8h constant potential test, no noticeable pitting behavior is shown on the surface because of the compact Sn/graphene coating. The addition of graphene promotes the nucleation of Sn metal and results in lower Sn oxidation.

Leaf-whorl inoculation with Sporisorium reilianum may overcome field resistance of maize.

Maize yield is threatened by increasing incidences of head smut disease caused by Sporisorium reilianum. To help breeders identify S. reilianum-resistant maize lines, the availability of efficient screening systems would be an advantage. Here we assessed maize lines with distinct levels of field resistance against head smut disease in greenhouse experiments using two different inoculation techniques. Addition of mixtures of mating-compatible sporidia to the soil at seedling stage of the plant did not lead to plant disease, and we could detect only marginal amounts of fungal DNA in apical meristems at eighteen days after sowing. Inoculation of the maize lines by leaf-whorl inoculation led to both high disease incidence and prominent levels of fungal DNA in apical meristems in all tested maize lines regardless of their field resistance levels. Thus, S. reilianum entering the plant via the leaf whorl can escape existing resistance mechanisms of currently known field-resistant maize lines. Since field-resistant lines are also resistant to inoculation via teliospore-contaminated soil, we propose teliospore addition to seeds at the time of sowing (rather than leaf-whorl inoculation of seedlings) combined with quantitative detection of fungal DNA in apical meristems, as an efficient screening procedure to discover field-resistant lines. However, screening maize plants for resistance against the leaf-whorl inoculation method might be promising for the discovery of novel resistance mechanisms needed to develop durably resistant maize lines.

Resistance monitoring of diamide insecticides and characterization of field-evolved chlorantraniliprole resistance among Chinese populations of the tomato pinworm Phthorimaea (=Tuta) absoluta (Lepidoptera: Gelechiidae)

The tomato pinworm, Phthorimaea (=Tuta) absoluta, is considered one of the most destructive and invasive insect pests worldwide, having developed significant resistance to many popular insecticides. In this study, we monitored the field resistance of P. absoluta populations from China to three diamide insecticides: flubendiamide, chlorantraniliprole, and cyantraniliprole. We found that one field population from Wuzhong City (WZ) exhibited high level of resistance to chlorantraniliprole. Using the WZ population and a susceptible reference strain (YN-S), we established a near-isogenic line (WZ-NIL) of P. absoluta with resistance to chlorantraniliprole. This strain also showed substantial cross-resistance to flubendiamide, and cyantraniliprole. Genetic analysis revealed that the inheritance of resistance to chlorantraniliprole in the WZ-NIL strain was autosomal and incompletely dominant. Additionally, the pesticide synergist piperonyl butoxide significantly inhibited chlorantraniliprole resistance by compromising P450 monooxygenase activity, which was significantly higher in the resistant strain. Furthermore, WZ-NIL had significantly prolonged developmental stages, lower pupation rates, reduced female fecundity, and lower egg hatchability than YN-S individuals. The fitness of WZ-NIL relative to YN-S was estimated to be 0.73, indicating significant fitness cost associated with chlorantraniliprole resistance. Rotating chlorantraniliprole with other insecticides that have different modes of action and degradation may be particularly useful for managing chlorantraniliprole resistance in P. absoluta.

SCREENING OF NEW SOYBEAN CULTIVARS AND CULTIVAR SAMPLES TOWARDS COMMON DISEASES IN KAZAKHSTAN

As part of the strategy to diversify cultivated areas and increase the profitability of agricultural enterprises, oilseeds, including soybeans, represent a promising direction for the development of the agricultural sector in the country. Kazakhstan is among the top 20 soybeanproducing countries globally. However, significant damage to soybean crops in the region was affected primarily by fungal diseases, leading to 20-50 % yield losses. This article presents field experiments conducted in the arid submontane agro-climatic Zhambyl region, focusing on the resistance of the global soybean collection. The study used 276 soybean varieties from Eastern Europe, Western Europe, North America, East Asia, and Kazakhstan. Screening of world soybean collection from 25 countries for field resistance towards various pathogens demonstrated a total of 197 soybean sources resistant to Cercospora sojina, 260 resistant to Septoria glycines, 76 resistant to Pseudomonas solanacearum, and 174 resistant to Peronospora manchurica. Among the tested samples, 24 soybean varieties of different origins exhibited high resistance to all pathogens, accounting for only 8.79% of those evaluated in the field during this experiment. These findings highlight the importance of identifying and utilizing disease-resistant soybean varieties to mitigate the impact of fungal diseases and improve soybean production in the region.

Dynamics of Bactrocera dorsalis Resistance to Seven Insecticides in South China.

Bactrocera dorsalis is a highly invasive and destructive pest distributed worldwide. Chemical insecticides remain the primary measure for their control; however, this species has already developed resistance to several insecticides. In recent years, there have been several reports of monitoring B. dorsalis resistance in China, but continuous monitoring results are lacking and do not even span a decade. In this study, we monitored the dynamics of resistance to seven insecticides among 11 geographically distinct Chinese populations of B. dorsalis (2010-2013; follow-up in 2023). The 11 populations were found to adapt rapidly to antibiotic insecticides (spinosad, emamectin benzoate, and avermectin), reaching high levels of insecticide resistance in several areas. Overall, a decreasing trend in resistance to organophosphorus insecticides (chlorpyrifos and trichlorfon) was observed, whereas pyrethroid (beta-cypermethrin and cyhalothrin) resistance trends were observed to both increase and decrease. The monitoring of field resistance among different B. dorsalis populations over the duration of this study is important for improving the efficiency and sustainability of agricultural pest management, and the results provide a scientific basis for the development of more effective resistance management strategies.

KUFRI DAKSH: A NEW WATER-USE EFFICIENT TABLE POTATO VARIETY WITH CROSS TOLERANCE TO HIGH TEMPERATURE STRESS

Kufri (K) Daksh is a main season, medium maturing, high yielding, water-use-efficient table potato variety that can be grown in regions with limited water availability. It is a clonal selection from the cross between CP1748, having high water use efficiency and LT-1 tolerating high temperature stress. Its plants are tall and vigorous with field resistance to late blight. Its tubers are white-cream (light yellow), ovoid with shallow to medium eyes and cream flesh with good keeping quality. It is fertilizer responsive and can yield 30-35 t/ha under low water (≤ 20%) availability in middle Gangetic plains. K Daksh has better trade-off for high CO2 gain and low water loss through optimization in plant canopy leaf area and plant height components. K Daksh can be an excellent choice to tolerate high temperature and water stress regimes in changing climatic scenarios.

Crystalline Oxygen-Modified Carbon Nitride Photocatalyst with Enhanced Internal Electric Field and Strong Resistance to Ionic Interference for Robust Seawater Splitting

Crystalline Oxygen-Modified Carbon Nitride Photocatalyst with Enhanced Internal Electric Field and Strong Resistance to Ionic Interference for Robust Seawater Splitting

250 μm Thick Detectors for Neutron Detection: Design, Electrical Characteristics, and Detector Performances

Solid State Detectors (SSD) are crucial for fast neutron detection and spectroscopy in tokamaks due to their solid structure, neutron-gamma discrimination, and magnetic field resistance. They provide high energy resolutions without external conversion stages, enabling compact array installations in the harsh environment of a tokamak. Research comparing diamond and 4H-SiC detectors highlights thickness as a key efficiency factor. A 250 μm SiC epilayer with low doping, grown using a high-growth-rate process, exhibits sharp interfaces and minimal defects, essential for neutron detectors. The study evaluates detector designs, and performance using a 4H-SiC substrate. Various detector designs, such as Schottky diodes and p/n diodes, are assessed via I-V and C-V measurements, addressing high depletion voltage challenges. Preliminary neutron irradiation tests validate detector functionality, energy resolution and confirming detector reliability.

Assessing the effect of provitamin a on maize field resistance to aflatoxin and fumonisin contamination

Vitamin A deficiency in sub-Saharan Africa is mainly being addressed through crop biofortification. Several high provitamin A (PVA) maize varieties have been released as part of these measures. However, these varieties are grown in areas where Aspergillus ear rot (AER) and Fusarium ear rot (FER) frequently occur, leading to contamination with mycotoxins, which in turn reduce the yield and grain quality. Chronic mycotoxin exposure leads to serious public health problems. Therefore, PVA maize varieties should be resistant to mycotoxin contamination. In a previous study, we generated 120 PVA hybrids by crossing 60 PVA inbreds and two testers with contrasting PVA content. Several inbreds resistant to aflatoxin were detected through laboratory-based kernel screening assays. In the current study, 21 PVA inbred lines with varying carotenoid content inoculated with toxigenic isolates of A. flavus and F. verticillioides were evaluated in field trials conducted at two locations in Nigeria for resistance to ear rots and mycotoxin production. Inbred lines resistant to AER, FER, aflatoxin and fumonisin contamination were identified. High PVA inbred lines were less susceptible to the ear rots, aflatoxin, and fumonisin than those with low PVA content. There were negative correlations between PVA content and each of AER (r = −0.28, P < 0.0001), FER (r = −0.37, P < 0.0001), aflatoxin (r = −0.15, P < 0.05), and fumonisin (r = −0.27, P < 0.0001). Three promising inbred lines were resistant to both aflatoxin and fumonisin. Moreover, the inbred TZI1715 combined resistance to AER, FER, aflatoxin, and fumonisin with desirable general combining ability for high β-carotene and total PVA content. These results suggest that the PVA biofortified maize developed to address vitamin A deficiency can also contribute to reduced exposure to aflatoxin and fumonisin.

Study on the detectability of CSRMT in archaeological exploration: taking the ancient city wall remains as an example

Although electromagnetic methods have made significant advances in archaeological exploration, there remains a lack of a method that offers both high operating efficiency and high resolution. This study introduces a novel approach derived from the Controlled Source Radio Magnetotelluric (CSRMT) method. The CSRMT method combines the efficient configuration of the Controlled Source Audio-magnetotelluric (CSAMT) method with enhanced resolution capabilities. To evaluate the efficacy of the new method in archeological exploration, we designed a series of models based on existing references related to ancient city wall remains. Using the 3D contraction integral equation method, we numerically simulated the high-frequency component of the CSRMT method, and then on, the Laterally Constrained Inversion technique is used to invert the data. The effectiveness of the method was assessed qualitatively and quantitatively through analysing the electromagnetic field and apparent resistivity of models with varying widths, heights, top burial depths, and resistivity. Our analysis revealed that models with shallower burial depth and greater height of the underground city wall exhibited higher resolution. The findings demonstrate that the CSRMT method can efficiently and accurately identify buried remains, making it a highly promising tool for archaeological exploration and warranting further development.

A novel Pik allele confers extended resistance to rice blast.

In the ongoing arms race between rice and Magnaporthe oryzae, the pathogen employs effectors to evade the immune response, while the host develops resistance genes to recognise these effectors and confer resistance. In this study, we identified a novel Pik allele, Pik-W25, from wild rice WR25 through bulked-segregant analysis, creating the Pik-W25 NIL (Near-isogenic Lines) named G9. Pik-W25 conferred resistance to isolates expressing AvrPik-C/D/E alleles. CRISPR-Cas9 editing was used to generate transgenic lines with a loss of function in Pik-W25-1 and Pik-W25-2, resulting in loss of resistance in G9 to isolates expressing the three alleles, confirming that Pik-W25-induced immunity required both Pik-W25-1 and Pik-W25-2. Yeast two-hybrid (Y2H) and split luciferase complementation assays showed interactions between Pik-W25-1 and the three alleles, while Pik-W25-2 could not interact with AvrPik-C, -D, and -E alleles with Y2H assay, indicating Pik-W25-1 acts as an adaptor and Pik-W25-2 transduces the signal to trigger resistance. The Pik-W25 NIL exhibited enhanced field resistance to leaf and panicle blast without significant changes in morphology or development compared to the parent variety CO39, suggesting its potential for resistance breeding. These findings advance our knowledge of rice blast resistance mechanisms and offer valuable resources for effective and sustainable control strategies.