Laser repair technology based on the DED principle is an advanced green remanufacturing technology that not only repairs the damaged parts but also restores their mechanical properties. Therefore, this technology has been widely used for the repair of key components in aircraft engine. However, due to the fact that the mechanical properties of the repaired zone are usually lower than those of the substrate, this limits the application of laser repair technology in the field of remanufacturing. Therefore, it is crucial to study the microstructure evolution and its influence on mechanical properties of the laser repaired object to improve the mechanical properties of the repaired zone and promote the widespread application of laser repair technology. In this study, the microstructure evolution and its effect on the aging hardening mechanism of laser repaired GH4169 alloy were investigated using SEM, EBSD and TEM techniques. The results show that the hardening mechanisms in the repaired zone are different from those in the substrate. The main hardening mechanisms in the repaired zone are strain hardening, elastic stiffness strengthening and aging hardening, while the hardening mechanisms in the matrix are grain refinement strengthening, aging hardening, strain hardening and texture strengthening. In addition, during the aging period, the texture of the matrix is mainly recrystallized Cube and Goss textures, while the texture of the repaired zone is mainly P texture, and the P grain nucleates and grows in the R-Copper grain.