Field Of Biochemistry Research Articles

Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is widely used as a crosslinker for fluorescence labeling of protein in the fields of biochemistry and food analysis. Many natural polysaccharides often contain some proteins or peptides that are very low in content but play a vital role in their biological function as well as technical applications. Determination of these low-content proteinaceous matters requires a highly sensitive and selective method. In this study, a methodological approach for investigations of the presence of proteinaceous material over the molar mass distribution (MD) of polysaccharides was developed using gum acacia (GA) as a model polysaccharide. EDC fluorescence-labeling method was modified by changing the pH (7, 9, and 11) of the solution for the analysis of low-content protein in food materials. Fluorescence spectroscopy and asymmetrical flow field-flow fractionation (AF4) were employed for characterizing the labeling efficiency and physiochemical properties of unlabeled and fluorescence-labeled GA. AF4 provided molar mass (M) and the radius of gyration (rG) of arabinogalactan (AG) and arabinogalactan protein complex (AGP) and determined the presence of proteinaceous matter over the MD. The labeling efficiencies of GA at pH 7, 9, and 11 determined by fluorescence spectroscopy were 56.5, 68.4, and 72.0%, respectively, with an increment of 15.5% when pH was increased from 7 to 11. The modified EDC fluorescence-labeling method allows highly sensitive and selective analysis of low-content proteinaceous matters and their distribution in natural polysaccharides.Graphical abstract

Inhalation Exposure to Atmospheric Nanoparticles and Its Associated Impacts on Human Health: A Review

Nanoparticles (NPs) are receiving an increasing attention from many scientific communities due to their strong influence on human health. NPs are an important marker of air pollution caused by a variety of natural and anthropogenic sources. Due to their ultrafine size, they can be suspended in the atmosphere for a long time and can thus travel larger distances and cause several health issues after exposure. A variety of NPs that are found in indoor and outdoor settings cause respiratory and cardiovascular diseases. Exposure to NPs through active and passive smoking and household and occupational subjection is reported with thick septum, shortness of breath, and a high level of interleukin protein and tumour necrosis factor (TNF-α) that cause tumour generation in the exposed population. This comprehensive review summarises NPs' source, exposure, and impact on different organ systems. Respiratory models (experimental and computational) used to determine the particle's deposition, airflow transport, and health impact are also discussed. Further, muco-ciliary escalation and macrophage activity, the body's clearance mechanisms after exposure to NPs, have been mentioned. An in-depth analysis of exposure to NPs through inhalation and their health impact has been provided with detailed insights about oxidative stress, inflammation, genotoxicity, and tumourigenicity. Overall, this review offers scientific evidence and background for researchers working in the field of epidemiology, biochemistry, and toxicological studies with reference to atmospheric nanoparticles.

Plasma membrane glycosphingolipid signaling: a turning point

Plasma membrane interaction is highly recognized as an essential step to start the intracellular events in response to extracellular stimuli. The ways in which these interactions take place are less clear and detailed. Over the last decade my research has focused on developing the understanding of the glycosphingolipids-protein interaction that occurs at cell surface. By using chemical synthesis and biochemical approaches we have characterized some fundamental interactions that are key events both in the immune response and in the maintenance of neuronal homeostasis. In particular, for the first time it has been demonstrated that a glycolipid, present on the outer side of the membrane, the long-chain lactosylceramide, is able to directly modulate a cytosolic protein. But the real conceptual change was the demonstration that the GM1 oligosaccharide chain is able, alone, to replicate numerous functions of GM1 ganglioside and to directly interact with plasma membrane receptors by activating specific cellular signaling. In this conceptual shift, the development and application of multidisciplinary techniques in the field of biochemistry, from chemical synthesis to bioinformatic analysis, as well as discussions with several national and international colleagues have played a key role.

Recent Developments in Free Energy Calculations for Drug Discovery.

The grand challenge in structure-based drug design is achieving accurate prediction of binding free energies. Molecular dynamics (MD) simulations enable modeling of conformational changes critical to the binding process, leading to calculation of thermodynamic quantities involved in estimation of binding affinities. With recent advancements in computing capability and predictive accuracy, MD based virtual screening has progressed from the domain of theoretical attempts to real application in drug development. Approaches including the Molecular Mechanics Poisson Boltzmann Surface Area (MM-PBSA), Linear Interaction Energy (LIE), and alchemical methods have been broadly applied to model molecular recognition for drug discovery and lead optimization. Here we review the varied methodology of these approaches, developments enhancing simulation efficiency and reliability, remaining challenges hindering predictive performance, and applications to problems in the fields of medicine and biochemistry.

Teaching Reform Practice of Biochemistry Course Based on Computer Technology

The course of biochemical engineering is an important content of modern scientific research and it has very important applications in the fields of biology and medicine. After entering the new era, with the rapid development of computer technology, the biochemistry field has the corresponding development, but the biochemistry curriculum teaching exposes many malpractices and the insufficiency in the traditional teaching mode. This paper analyzes the existing problems in the teaching of biochemistry course, expounds the teaching reform content of biochemistry course and puts forward specific ideas and practical methods for reference of relevant personnel.

Gaucher disease: achievements and prospects

Gaucher disease (GD) is the most common lysosomal storage disorder, resulting from a deficiency in the activity of a lysosomal enzyme glucocerebrosidase, which is involved in the catabolism of sphingolipids. The phenomenal progress in understanding the pathogenesis and development of specific therapy of this disease over the past 60 years dramatically changed the clinical phenotype of GD, turning a severe progressive disorder into an asymptomatic metabolic defect. The evolution of the understanding of GD associated with fundamental discoveries in the field of cell biology, biochemistry and genetics may be of interest to a wide audience as a model of the effective work of the scientific community in the treatment of rare metabolic pathology.

Droplet Microfluidics for Food and Nutrition Applications.

Droplet microfluidics revolutionizes the way experiments and analyses are conducted in many fields of science, based on decades of basic research. Applied sciences are also impacted, opening new perspectives on how we look at complex matter. In particular, food and nutritional sciences still have many research questions unsolved, and conventional laboratory methods are not always suitable to answer them. In this review, we present how microfluidics have been used in these fields to produce and investigate various droplet-based systems, namely simple and double emulsions, microgels, microparticles, and microcapsules with food-grade compositions. We show that droplet microfluidic devices enable unprecedented control over their production and properties, and can be integrated in lab-on-chip platforms for in situ and time-resolved analyses. This approach is illustrated for on-chip measurements of droplet interfacial properties, droplet–droplet coalescence, phase behavior of biopolymer mixtures, and reaction kinetics related to food digestion and nutrient absorption. As a perspective, we present promising developments in the adjacent fields of biochemistry and microbiology, as well as advanced microfluidics–analytical instrument coupling, all of which could be applied to solve research questions at the interface of food and nutritional sciences.

Integrating Antiracism, Social Justice, and Equity Themes in a Biochemistry Class

A recent push toward addressing antiracism in science reveals a need to integrate discussions of racism, social justice, and equity into undergraduate STEM classes. Toward this end, in Fall 2020, a theme of “Racism is a Public Health Emergency” was incorporated as an overlay for an existing undergraduate biochemistry curriculum. This theme was used throughout the entire semester and involved discussions of racism, social justice, and equity that were directly integrated with the science content of the course. Students enrolled in the class were surveyed at the end of the semester regarding their perceptions of integrating discussions of racism, social justice, and/or equity into chemistry courses. Students indicated that the inclusion of the overlay increased the relevance of the course material, indicated a high level of understanding of the impact of bias and race on the field of biochemistry, and expressed overwhelmingly positive views regarding future inclusion of similar themes of equity and antiracism in chemistry courses. Additionally, student scores and answers on common final exam questions were compared to previous semesters, revealing that the inclusion of this overlay did not negatively impact coverage of biochemistry content.

Standing with giants: W.J. Whelan, a catalyst for international cooperation via the Miami Winter Symposium and the IUBMB

The Miami Winter Symposium, now in its fifth decade, appeared with various names in its early offerings. Dr. William J. Whelan, Professor and Chairman of Biochemistry and Molecular Biology at the University of Miami Miller School of Medicine started the Symposium in 1967 and continued to organize the annual meeting for many years. In 1983, I relocated from Vanderbilt University, where I was Professor of Biochemistry, to the University of Miami as Director of an interdepartmental research and clinical group, taking my primary faculty appointment in Biochemistry and Molecular Biology. Working with Dr. Whelan and others on the Organizing Committee for the 1986 Miami Winter Symposium, I recommended my friend and former colleague at Vanderbilt University, the late Dr. Stanley Cohen, for the Feodor Lynen Lecture. This proposal was met with enthusiasm by the Organizing Committee, as was the suggestion by Dr. Whelan that Drs. Herbert W. Boyer of the University of California at San Francisco and Stanley N. Cohen of Stanford University be co-recipients of the Symposium's Distinguished Service Award. It was a memorable meeting, and, when rummaging through some old files that somehow had survived laboratory and home moves, I found two poignant photographs taken at a 1986 Symposium reception. In Figure 1, I am shown with Drs. Whelan, Boyer, and Stanley N. Cohen, and Figure 2 captures Dr. Boyer flanked by the two Stanley Cohens. If I remember correctly, this was the first meeting of the Vanderbilt and the Stanford Stanley Cohens. Of note, the “Vanderbilt Stanley Cohen” is holding his signature corncob pipe. The work of Dr. Whelan and the three distinguished scientists honored at the 1986 Miami Winter Symposium was at the forefront in broadening the burgeoning field of biochemistry and extending it to include genetic engineering and molecular & cell biology. Their pioneering concepts and applications remain in wide use today. The impact of these four individuals on the life sciences and biomedicine has been immense, and over the years this foursome garnered many prestigious awards. To name but a few of these recognitions, Herbert Boyer, Stanley Cohen, and Stanley N. Cohen each received a National Medal of Science Award, and each was recognized with an Albert Lasker Award for Basic Medical Research. In addition, Herbert Boyer was a co-founder of Genentech, a highly successful biotech company. The Vanderbilt Stanley Cohen received the Fred Conrad Koch Award of the Endocrine Society and the Nobel Prize in Physiology or Medicine the same year he gave the Feodor Lynen Lecture in Miami, the Nobel Prize being shared with his former collaborator, Dr. Rita Levi-Montalcini. Bill Whelan was elected a Fellow of the Royal Society, was an honorary Member of the Royal College of Physicians of London, became a Fellow of the American Association of Academic Scientists, and received an honorary DSc. degree from La Trobe University, Australia. Complementing his leadership role in the annual Miami Winter Symposium and his original research on the importance of glycogenin as a primer for the initiation of glycogen synthesis, Bill was also effective in integrating biochemistry at the international level and serving as the founder and leading force of a number of biochemistry journals and societies. These accomplishments included: service as the first Editor-in-Chief of the FASEB Journal, BioEssays, and Trends in Biochemical Sciences; Founding Editor of FEBS Letters; Executive Editor of Archives of Biochemistry and Biophysics; Editorial Board Member of the Biochemical Journal; cofounder and First Secretary General of FEBS and PAABS; General Secretary and President of the IUBMB; and founder and, along with Dr. Angelo Azzi, co-Editor-in-Chief of IUBMB Life, the latter being a position he held from 2000 to 2020.1 Without question, in addition to being an outstanding biochemist, Dr. Whelan was a quintessential catalyst in promoting not only biochemistry and the burgeoning field of molecular biology, but also the international cooperation of life scientists. Each of these biochemists and molecular biologists continued their groundbreaking research for many years following the 1986 Symposium. It was a pleasure for me, as a mid-career scientist, to have had the unique opportunity to be in the company of such accomplished individuals; indeed, these were an “awesome foursome.” The author declares no conflict of interest.

Deciphering the Interaction of a Macrocyclic Tetranuclear Zn(II) complex with Biomimicking Surfactant: A Case Study using Cetyltrimethylammonium Bromide

The detection of various biologically important cationic and anionic species has led to the synthesis of various Zinc (II) Schiff base complexes, which bear a large application in catalysis and sensors. A recently reported study has indicated a synthesis of a 48 membered macrocyclic Zn(II) Schiff base complex, which functions as a dual anion sensor (DAS) containing a protonated amine group. In the present investigation, an attempt has been made to study the interaction of the Zn tetranuclear complex (Zn-TCom), a neutral complex, with a cationic surfactant cetyltrimethylammonium bromide (CTAB), employing steady-state absorption and fluorescence studies. The change in the photophysical properties of the fluorophore is studied on binding with the surfactant. The steady-state absorption and emission studies reveal that the polarity of the surrounding of the fluorophore has been modified upon binding with CTAB. A study on the alteration of polarity during the interaction of Zn-TCom with the surfactant is studied by comparing the fluorescence behavior of the probes in a micro heterogeneous environment in a mixture of dioxane and water of varying composition. The binding constant magnitude (analyzed using the Hildebrand equation) manifests that the Schiff base binds to the micellar compartment. These results are manifested to enhance the potential application of the macrocyclic complex in the field of biochemistry. Furthermore, quantum chemical calculations are undertaken to correlate the molecular structure of Zn-TCom with the associated frontier molecular orbitals. The parameters like the energy of HOMO, LUMO and the associated Mulliken charges with respect to individual elements are computed using the single point geometry. The studied reports are correlated with the feasibility of binding with bio mimicking environment or bio proteins for selective application as drug carriers.

DIGESTIVE DISORDERS IN THE ORAL CAVITY

The progress made in fundamental medical research over the past decades, the scientific acquisitions in the field of genetics, molecular biology and biochemistry in relation to the explosive development of investigative technologies have revolutionized the clinical approach to many pathological entities, practically opening a new era in the evolution of clinical medicine. Dental medicine, as a science, feels the massive impact of the needs for knowledge and relaunches the interest of research in all its subspecialties. From this perspective, these are legitimized not only through the crisis in managing the immense volume of information, but also through the openings offered to the framework of conceptualizing and defining the identity of this branch of medicine, related to the need to particularize the specific problems in this discipline. Digestion is a fundamental process in the survival of an organism. It begins in the oral cavity, where the bolus is formed, and continues in the stomach, forming the chyme, which then reaches the small intestine and transforms into the chyle. Through mastication, the surface of food increases, thus the enzymes are able to act more easily on the substrate. The first enzymes to act on food are the salivary ones - salivary amylase, lingual lipase. Mastication is regulated through the contact of food with receptors in the oral cavity. These will send impulses by way of the trigeminal nerve towards the centre of mastication - located in the bolus. From the bolus, they will start the signals on the efferent pathway (trigeminal, hypoglossal and facial nerves) that will reach the masticatory muscles. Mechanical digestion (mastication) can thus begin. Mechanical digestion in the oral cavity results from mastication. During mastication, the food is manipulated by the tongue, crushed by the teeth and mixed with saliva. Concomitant with mechanical digestion, the chemical digestion takes place through the action of saliva. There are two types of salivary glands in the oral cavity: large glands - parotid, sublingual, submandibular and small glands - disseminated throughout the oral cavity. Within 24 hours, up to 1.5 liters of saliva are secreted, 99% of which is represented by water. The remaining 1% consists of enzymes, mucus, nitrogen content. After finalizing mastication, deglutition begins. This mechanical process consists of thrusting the bolus from the mouth towards the stomach, using the esophagus.

Study on a Plasmonic Tilted Fiber Grating-Based Biosensor for Calmodulin Detection.

Tilted fiber Bragg grating, which has the advantages of both fiber Bragg grating and long-period fiber grating, has been widely studied for sensing in many fields, especially in the field of biochemistry. Calmodulin, which has a wide distribution in eukaryotes, can regulate several enzymes such as adenylate cyclase and guanylate cyclase and mediates several cellular processes such as cell proliferation and cyclic nucleotide metabolism. The abnormal levels of calmodulin in the body will result in serious effects from metabolism to nerve growth and memory. Therefore, it is important to measure the calmodulin concentration in the body. In this work, we propose and experimentally demonstrate a plasmonic tilted fiber Bragg grating-based biosensor for calmodulin detection. The biosensor was made using an 18° tilted fiber Bragg grating with a 50 nm-thick gold nanofilm coating the surface of the fiber, and transient receptor potential channels were bonded onto the surface of the gold nanofilm to serve as bio-detectors for calmodulin detection. Experimental results showed that the limit of detection using our biosensor was 0.44 nM. Furthermore, we also demonstrated that the interaction between calmodulin and transient receptor potential channels was quite weak without calcium in the solution, which agrees with the biology. Our proposed biosensor has a simple structure, is easy to manufacture, and is of small size, making it a good choice for real-time, label-free, and microliter-volume biomolecule detection.

Ultra-wide sensing range plasmonic refractive index sensor based on a two-dimensional circular-hole grating engraved on a gold film

We propose a simple structure with a two-dimensional (2D) circular-hole grating engraved on a gold film for ultra-wide range plasmonic refractive index (RI) sensing. The electromagnetic field distributions and reflection spectra of the structure are obtained using the finite-difference time-domain approach for resonance mode analysis and sensing capability characterization. The simulation results show that a high-quality surface plasmon resonance with a narrow spectral bandwidth and strong dip strength can be excited using a 2D grating after compensating for a suitable optical momentum. The structure exhibits a high sensitivity of 1131 nm/RIU and stable sensing performance over an ultra-wide RI range of 1.0–2.0. An improved figure of merit is used to more comprehensively characterize the structural sensing performance. This sensor has the potential to be widely used in the field of biochemistry because of its simple preparation, high sensitivity, and ultra-wide sensing range.

CКОРОСТНОЙ СПЕКТРОФЛУОРИМЕТР СФЛ-С

An experimental multifunctional high speed registration spectrofluorimeter SFL-S has been developed at the Institute for Biological Instrumentation of the Russian Academy of Sciences. The spectroflu-orimeter is designed to measure excitation and emission spectra of solutions and suspensions of bio-logical molecules and cells in the ultraviolet, visible, and near infrared spectral regions in the course of fundamental and applied research in the field of physicochemical biology, biophysics, biochemistry, and medicine. Features of the instrument are: the possibility of high-speed registration of spectra by means of multichannel system (32 channels, more than 20 spectra per second); the ability to control the change of the temperature of the solution in the measuring cell according to a given algorithm (0 to 100 °C); the possibility of microtitration of the solution in the measuring cell using an automatic microtitrator.

Evolution of Cancer Vaccines-Challenges, Achievements, and Future Directions.

The development of cancer vaccines has been intensively pursued over the past 50 years with modest success. However, recent advancements in the fields of genetics, molecular biology, biochemistry, and immunology have renewed interest in these immunotherapies and allowed the development of promising cancer vaccine candidates. Numerous clinical trials testing the response evoked by tumour antigens, differing in origin and nature, have shed light on the desirable target characteristics capable of inducing strong tumour-specific non-toxic responses with increased potential to bring clinical benefit to patients. Novel delivery methods, ranging from a patient’s autologous dendritic cells to liposome nanoparticles, have exponentially increased the abundance and exposure of the antigenic payloads. Furthermore, growing knowledge of the mechanisms by which tumours evade the immune response has led to new approaches to reverse these roadblocks and to re-invigorate previously suppressed anti-tumour surveillance. The use of new drugs in combination with antigen-based therapies is highly targeted and may represent the future of cancer vaccines. In this review, we address the main antigens and delivery methods used to develop cancer vaccines, their clinical outcomes, and the new directions that the vaccine immunotherapy field is taking.

Talking in Circles: Undergraduate Biochemistry Student Conceptions About Biochemical Pathway Regulation

For many undergraduate students, introductory biochemistry courses are difficult because they involve novel abstract concepts that require assimilation of new knowledge with pre-existing notions from biology and chemistry courses. According to cognitive load theory, students use pre-existing schemas to fit this new knowledge onto the framework of what they already know. Extraneous cognitive load can interrupt this process by overwhelming working memory. Misconceptions increase cognitive load and interrupt the mental framework, making it difficult for students to master course material. In the field of biochemistry, little research exists on how students understand and learn about the threshold concept of Biochemical pathway dynamics and regulation. Initially a non-empirical pilot study was conducted with students who had completed an undergraduate biochemistry I course. These participants were interviewed using the three-phase single interview technique (3-PSIT), which probes the student's knowledge by creating cognitive dissonance using visual representations. Preliminary inductive content analysis of the transcribed text from this pilot study identified patterns and categories of (mis)conceptions among students. From this, student responses were collected from assessments informed by the pilot study. These responses were used to further identify patterns of (mis)understanding with regards to pathway regulation. The information gathered here enables development of learning assessments and pedagogical techniques to better teach the complex concepts surrounding biochemical pathway regulation.

A Narrative Curriculum Using the Primary Literature to Teach Biochemistry

The interdisciplinary field of biochemistry and molecular biology (BMB) is expansive, and the pace of scientific discovery is dizzying. This makes teaching the entirety of BMB - even in three semesters - nearly impossible. Instead, BMB education has moved to competencies over content coverage, as enumerated in ASBMB's Foundational Concepts and Skills and in AAAS's Vision and Change. Focusing on a checklist of competencies can still lead students to learning a collection of disparate facts rather than integrating this knowledge across domains. The second-year Biochemistry course at Juniata College uses acetylcholine neurotransmission as a central narrative from which content is constructed. Beginning with texts from the 1700s, students work in groups to iteratively interrogate primary scientific literature by (i) developing specific questions, (ii) using resources to find answers to those questions, (iii) communicating those answers with the peers in their team, and (iv) using the answers to interpret data and figures. In the process, students teach themselves core biochemical concepts interwoven with mathematics, physiology, chemistry, and physics and set in cultural and historical context. Here, I present evidence that this social constructivist approach leads to gains in inclusivity and self-efficacy with respect to teamwork, curiosity, and scientific literacy. Moreover, the approach has led to several collaborative projects, including translations of 19th-century texts from German and French to English. The design of this course stretches BMB education beyond the limits of traditional content acquisition and into new and exciting ways of engaging students and encouraging them to take control of their own education.

Development of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator

The recognition and detection of proteins has an important role in the fields of biochemistry and medicine in term of diagnosis and prognosis. In this study, molecularly imprinted cryogel (MIP) was synthesized for the selective recognition of Cytochrome-c (Cyt-c) by cryopolymerization techniques with the lanthanide-chelate approach using (MAAP)2-Ce(III) as complex functional monomer and Cyt-c as a template protein. The incorporation of template with monomer was characterized by near- infrared (Near-IR) spectroscopy. The binding capacity was optimized in accordance with numerous experimental parameters including pH, initial Cyt-c concentration, flow rate, temperature and ionic strength. As a result, the maximum binding capacity reached to 98.33 mg g−1 in a buffer system with a pH of 6.0. Beside of the excellent reusability performance of prepared MIP, it was successfully applied to separation of template Cyt-c in the existence of hemoglobin (Hb) and myoglobin (Mb) chosen as interfering proteins. The relative selectivity constants of the Cyt-imprinted polymer were determined as 13.84 for the Cyt/Mb pair and 16.05 for the Cyt/Hb pair. Consequently, a high selectivity, simple and cheap strategy for efficient separation of proteins was provided with this novel MIP cryogel.

Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research

Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.

Some peculiarities of spectral properties of various compounds of adenine nucleotide and polynucleotide

DNA is vitally important macromolecule and often used in various fields of biochemistry, biology and medicine as a polymer or model compound or as a part of drug delivery systems. In numerous studies the DNA fluorescence and phosphorescence emission was observed and investigated. It is known that adenine possesses the lowest triplet electronic energy level among the other bases of DNA (guanine, tymidine, cytosine), and DNA phosphorescence emission is mainly connected with adenine-tymidine (AT-) complexes emission. In this article, various types of adenine-containing compounds have been studied by optical methods, and their luminescence spectra were analyzed. The UV–Vis spectra as well as fluorescence and phosphorescence spectra of water solutions of AMP, PolyA and deoxyadenosine at temperatures 300 K and 77 K were studied. The dramatic difference in phosphorescence spectra structures and intensities of adenine-containing compounds in dependence on their composition and structure in number of cases were revealed. The reasons of this phenomenon were discussed.