In order to expand the population size of the rare but near-threatened wild animals, captive-rearing-restocking program was generally adopted worldwide; meanwhile, antimicrobics were remaining in different environmental compartment at sub-inhibitory concentrations(SIC) due to long-term over-prescription and abuse of them, which impact the metabolisms of various microorganisms, often with drastic consequences in terms of ARGs enrichments; however, there are as yet no studies on the risk of ARGs horizontal transfer caused by the microbe residing in the gut of specific wildlife in the setting (soil) where they are artificial rearing and breeding.To assess the risk mentioned above, Escherichia coli strains were isolated from the fresh feces of wild Hume's pHeasants which lived in the original habitat and the overlapping strains were rejected by ERIC-PCR assay. The Kirby-Bauer (KB) method was used to determine the drug resistance, and plasmids were identified by PCR-based replicon typing (PBRT). Effects of SIC of 3 antimicrobials on the horizontal transfer ratio (HTR) of ARGs were measured with co-cultured of E. coli and Klebsiella oxytoca in soil containing different concentrations of antimicrobics using our patented device. The results were that 17 ERIC fingerprints of E.coli were obtained (referred to as E1-E17) and 100%, 88.24% and 76.47% were sensitive to Aztreonam and Meropenem, Ciprofloxacin, Ofloxacin respectively, so the rational use of these antimicrobics should effectively inhibit these bacteria, this was important for the domestication of Syrmaticus humiae.In the E15, FIC, FrepB, FIB, HI1 plasmids and tetracycline resistance gene tetB were detected; in the K. oxytoca, the FIB; in the tranconjugant (ConI) that derived from E15 and K. oxytoca, FrepB, FIB and tetB. These suggested that Dox (Doxycycline)resistance of strain E15 and Con-I was conferred by tetB and it was transformed from E15 to K. oxytoca with the FrepB plasmid by transconjugation.In Luria-Bertani medium, the HTR of tetB transformed from E15 to K. oxytoca was positively correlated with SIC of Kan (P < 0.05), but was not significantly correlated with that of Sm2 and Dox. In the soil conditions where Wild Hume's pheasants were artificial Rearing and Breeding, SIC of Kan were not well-correlated with the HTR of tetB, but that of the tetB in soil supplemented with 1/4 MIC of Sm2 was almost 2.5 times of that in the soil which was not added with Sm2.Therefore, the mobile genetic elements carried by E. coli in wild S. Humiae could disseminate ARGs as the bird were domesticated, hence, assess such a risk is necessary in the future when adopting captive-rearing-restocking program for expanding the population size of the rare but near-threatened wild animals.