The role of blood clot in tissue repair has been identified for a long time whereas its participation in the integration between implants and host tissues has attracted attentions only in recent years. In this work, the mesoporous silica thin film (MSTF) with either vertical or parallel orientation was deposited on titania nanotubes (TNT) surface, resulting in superhydrophilic nanoporous surfaces. Through proteomic analysis of blood plasma adsorption, the MSTF coating could significantly increase the acidic proteins abundance and the coagulation factors (XII and XI) adsorption, with the help of cations (Na+, Ca2+) binding. As a consequent, both platelets activation and the blood clot formation were significantly enhanced on MSTF surface with more condensed fibrin networks. Two classical growth factors of PDGF-AB and TGF-β were enriched in the blood clots from MSTF surface, which accounted for robust osteogenesis both in vitro and in vivo. In conclusion, the study demonstrates that the MSTF may be a promising coating to enhance osteogenesis through modulating blood clot formation.