Rice Fertilizer Research Articles

Maternally expressed OsFERTILIZATION INDEPENDENT ENDOSPERM1 regulates seed dormancy and aleurone development in rice.

Seed dormancy, an essential trait for plant adaptation, is determined by the embryo itself and the surrounding tissues. Here, we found that rice (Oryza sativa) FERTILIZATION INDEPENDENT ENDOSPERM1 (OsFIE1) regulates endosperm-imposed dormancy and the dorsal aleurone thickness in a manner dependent on the parent of origin. Maternally expressed OsFIE1 suppresses gibberellin (GA) biosynthesis in the endosperm by depositing trimethylation of lysine 27 on histone H3 (H3K27me3) marks on GA biosynthesis-related genes, thus inhibiting germination and aleurone differentiation. Knockout of rice GA 20-oxidase1 (OsGA20ox1) alleviated the phenotypic defects in osfie1. The aleurone-positive determinant Crinkly 4 (OsCR4) is another target of the OsFIE1-containing Polycomb repressive complex 2 (PRC2). We found that OsFIE1 plays an important role in genomic imprinting in the endosperm of germinating seeds, particularly for paternally expressed genes associated with H3K27me3. The increased aleurone thickness of osfie1 substantially improved grain nutritional quality, indicating that the osfie1 gene may be utilized for breeding nutrient-enriched rice. The findings provide insights into the essential roles of PRC2-mediated H3K27me3 methylation in the acquisition of seed dormancy and endosperm cell differentiation in rice.

Identification and Gene Fine Mapping of the Bisexual Sterility Mutant Meiosis Abnormal Bisexual Sterility 1 in Rice

Exploring the genes regulating rice fertility is of great value for studying the molecular mechanisms of rice reproductive development and production practices. In this study, we identified a sterile mutant from the mutant library induced by ethyl methanesulfonate (EMS), designated as meiosis abnormal bisexual sterility 1 (mabs1). The mabs1 mutant exhibits no phenotypic differences from the wild-type during the vegetative growth phase but shows complete sterility during the reproductive growth phase. Phenotypic observations revealed that both pollen and embryo sac fertility are lost in mabs1. Notably, in mabs1, the development of the anther inner and outer walls, tapetum degeneration, and callose synthesis and degradation all proceed normally, yet meiosis fails to form normal tetrads. Genetic analysis indicated that this mutant trait is controlled by a single recessive nuclear gene. By constructing a genetic segregation population, we successfully mapped the MABS1 gene to a 49 kb region between primer markers Y7 and Y9 on chromosome 1. Resequencing revealed a single-nucleotide substitution in the exon of the LOC_Os01g66170 gene, which resulted in a change from Valine to Isoleucine. Subsequent sequencing of this locus in both wild-type and mabs1 mutants confirmed this mutation. Therefore, we have identified the gene at LOC_Os01g66170 as a candidate for MABS1, a previously unreported novel gene involved in rice meiosis. Through RT-qPCR, we found that the expression levels of multiple meiosis-related genes were significantly changed in the mabs1 mutant. Therefore, we believe that MABS1 is also involved in the process of rice meiosis. This study lays the groundwork for a functional study of MABS1.

Impact of Foliar Spray of Zinc in Nano Form on Lentil Grown Under Residual Fertility of Preceding Rice

Impact of Foliar Spray of Zinc in Nano Form on Lentil Grown Under Residual Fertility of Preceding Rice

The E3 ligase OsHel2 impedes readthrough of stalled mRNAs to regulate male fertility in thermo-sensitive genic male sterile rice.

Heterosis is extensively utilized in the two-line hybrid breeding system. Photo-/thermo-sensitive genic male sterile (P/TGMS) lines are key components of two-line hybrid rice. TGMS lines containing tms5 have significantly advanced two-line hybrid rice breeding. We cloned the TMS5 gene and found that TMS5 is a tRNA cyclic phosphatase that can remove 2',3'-cyclic phosphate (cP) from cP-ΔCCA-tRNAs for efficient repair to ensure maintenance of mature tRNA levels. tms5 mutation causes increased cP-ΔCCA-tRNAs and reduced mature tRNAs, leading to male sterility at the restrictive temperatures. However, the regulatory network of tms5-mediated TGMS remains to be elucidated. Here, we identified that an E3 ligase OsHel2 cooperates with TMS5 to regulate TGMS at the restrictive temperatures. Consistently, both the accumulation of 2',3'-cP-ΔCCA-tRNAs and insufficiency of mature tRNAs in tms5 mutant were largely recovered in the tms5 oshel2-1 mutant. A lesion in OsHel2 results in partial readthrough of the stalled sequences, thereby evading ribosome-associated protein quality control (RQC) surveillance. Our findings reveal a mechanism by which the OsHel2 impede readthrough of stalled mRNA sequences to regulate male fertility in TGMS rice, thus providing a paradigm for investigating how disorders in the components of the RQC pathway impair cellular functions and lead to diseases or defects in other organisms.

Peningkatan Kapasitas Petani Melalui Pelatihan Pemupukan Tanaman Padi di Provinsi Sumatera Utara

To increase farmers knowledge and skills in managing rice fertilization, the government and related institutions have implemented various fertilizer training programs. This research aims to evaluate the effectiveness of rice fertilizer training in increasing the capacity of farmers in North Sumatra Province. This research was carried out at UPT. Agricultural Training and Mechanization for the Food Security, Food Crops, and Horticulture Service of North Sumatra Province on February 20–23, 2024. This research was carried out on 30 training participants from 12 districts in North Sumatra. The data collected consists of primary data consisting of respondent characteristics and pre-test and post-test results. The data were analyzed with the Minitab applications of tware using non-parametric statistical analysis, namely the Wilcoxon Match Pairs Test with a significance level of 0.05. The increase in knowledge of farmers who took part in rice fertilization training was 32.2%. In general, attitudes and responses are classified as high, namely the benefits of the material provided are 100%, the ease of the material to be appliedis 100%, the excessprofits obtained in applying the material are 100%, the suitability of the material with farming needsis 100%, liking orinterest in the material provided was 100%, the desire to apply the material provided was 83.3%, and the plan to implement the material provided was 80%. Keywords: training, pretest, posttest, fertilizing rice plants

Effects of nutritional stress on soil fertility and antioxidant enzymes of rice in different growth periods.

Stress in plants denotes the detrimental impact of alterations in external environmental conditions on regular plant growth and development. Plants employ diverse mechanisms to mitigate or evade nutritional stress-induced damage. In order to investigate the physiological response mechanism of plants to nutritional stress and assess its impact on soil nutrient content and antioxidant enzyme activity in rice, a field experiment was conducted applying five treatments: control, nitrogen (N) deficiency, phosphorus (P) deficiency, potassium (K) deficiency, and full fertilization. Rice leaf and soil samples were concurrently gathered during both the vegetative and reproductive growth stages of rice. Analysis was conducted on soil N, P, and K levels, as well as leaf antioxidant enzyme activities, to investigate the impact of nutrient stress on rice antioxidant enzymes and soil fertility. The research findings indicate that full fertilization treatment enhanced the agronomic properties of the soil compared to the control treatment. In the N-deficiency treatment, reactive oxygen species (ROS) levels increased by 16.53-33.89% during the reproductive growth period compared to the vegetative growth period. The peroxidase (POD) activity decreased by 41.39% and superoxide dismutase (SOD) activity increased by 36.22% under K-deficiency treatment during the reproductive growth period compared to the vegetative growth period. Consequently, applying N and P fertilizer during the vegetative growth period can decrease membrane lipid peroxidation levels by 7.34-72.53%. The full fertilization treatment markedly enhanced rice yield compared to other treatments and increased the Nitrogen activation coefficient (NAC) and Phosphorus activation coefficient (PAC) in the soil, while decreasing the PAC. Elevating NAC levels can stimulate the activity or content of PRO, MDA, and RPS during the vegetative growth stage, whereas in the reproductive growth stage, it will decrease the content of ROS, PRO, and MDA. This data offers valuable insights and theoretical support for nutritional stress research.

Yield-scaled greenhouse gas emissions associated with the use of stabilized nitrogen fertilizers in subtropical paddy rice

Yield-scaled greenhouse gas emissions associated with the use of stabilized nitrogen fertilizers in subtropical paddy rice

Study the Effect of Integrated Nutrient Management with Bio-inoculants on Yield Attributes and Yields of Rice (Oryza sativa L.)

The experiments were conducted during the kharif season of 2021-22 and 2022-23 at the S.I.F, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur. The experiment consists of 18 treatment combinations in randomized block design with three replications of different combinations of inorganic fertilizer, organic and bio-inoculants in rice. Based on results emanated from the investigation it can be concluded that among the productivity parameters viz: The statistically maximum grain yield (q ha-1), straw yield (q ha-1) and biological yield (q ha-1) were found (47.38, 47.56 q ha-1), (66.82, 67.22q ha-1 ) and (114.20, 114.78 q ha-1 ) in 2021-22 and 2022-23 respectively in the treatments recorded in the treatment T17 - 100% NPK+ 5 ton ha-1 FYM + Azolla (300 kg ha-1) + S (30 kg ha-1) + Zinc (5 kg ha-1) during both years of experimentation and the plant growth traits viz: panicle length (28.93cm), panicle weight (g) (5.86g) number of grain panicle-1 (127) grains weight panicle-1 (4.11) and test weight (g) (24.97g) were recorded statistically maximum with the treatment T17 -100% NPK+ 5 ton ha-1 FYM + Azolla (300 kg ha-1) + S (30 kg ha-1) + Zinc (5 kg ha-1) during the experimentation.

Disruption of Energy Metabolism and Reactive Oxygen Species Homeostasis in Honglian Type-Cytoplasmic Male Sterility (HL-CMS) Rice Pollen

Honglian type-cytoplasmic male sterility (HL-CMS) is caused by the inter-communication between the nucleus and mitochondria. However, the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remains unclear. In this study, we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line NIL-Rf6 using hematoxylin and eosin staining as well as 4ʹ,6-diamidino- 2-phenylindole (DAPI) staining. HL-CMS is characterized by gametophytic sterility, where the aborted pollen grains are empty and the tapetal layer remains intact. Transmission electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage, revealing that significant mitochondrial alternations, characterized by the formation of 'large spherical mitochondria', occurred at the binucleate stage in the YA line. Additionally, metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid pathways. Notably, the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species (ROS) levels. Therefore, we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites, with impaired mitochondrial energy production and reduced flavonoid biosynthesis being the main causes of ROS accumulation and pollen abortion in rice.

Evaluating the effect of organic NPK granules on yield, nutrient uptake, post-harvest soil fertility and soil microbial population in rice (Oryza sativa L.)

Rice is the most important and staple food crop for more than two third of population of India. India is the second largest rice producing country in the world after China. Although, rice planted area in India is 40 per cent higher than in China, Indian rice production is 30 percent below Chinese production because of lower yields. Among several constraints of production, nutrient management plays a key role in realizing sustainable yield from any given cultivation practices. Integrated nutrient management system plays a vital role in balancing the soil fertility and plant nutrient supply to an optimum level through the judicious and efficient use of chemical fertilizers, green manure, FYM and biofertilizers leading to an eco-friendly approach and economically viable solution for this problem. Continuous integrated use of organic manures with chemical fertilizers would be quite promising in assessing the sustainability of a cropping system vis-à-vis monitoring the soil properties. To investigate further, a field experiment was conducted at the Experimental Farm, Department of Agronomy, Annamalai University, Annamalai Nagar, Cuddalore district of Tamil Nadu from June to September 2023 with a key objective of evaluating the effect of combined application of graded levels of conventional fertilizers along with organic NPK granules in lowland rice. The experiment was laid out in randomized block design with seven treatments comprised of different levels of conventional fertilizers along with organic NPK granules. Among the nutrient management evaluated, all the yield attributes of lowland rice viz., grain yield, straw yield, uptake of nutrients and post-harvest soil available nutrients were significantly influenced by the application of 70% NPK through synthetic fertilizers and 30% NPK through organic granules. Combining conventional fertilizers along with organic NPK granules improved nitrogen use efficiency via metabolite production, enzyme activity stimulation, and microbial rhizosphere interactions significantly enhancing the yield attributes and yield.

Pollen-Expressed Plant U-Box Protein, OsPUB14 Involves in Rice Fertility and Degrades OsMTD2

Pollen-Expressed Plant U-Box Protein, OsPUB14 Involves in Rice Fertility and Degrades OsMTD2

Kinesin-1-like protein PSS1 is essential for full-length homologous pairing and synapsis in rice meiosis.

The pairing and synapsis of homologous chromosomes are crucial for their correct segregation during meiosis. The LINC (Linker of Nucleoskeleton and Cytoskeleton) complex can recruit kinesin protein at the nuclear envelope, affecting telomere bouquet formation and homologous pairing. Kinesin-1-like protein Pollen Semi-Sterility1 (PSS1) plays a pivotal role in male meiotic chromosomal behavior and is essential for fertility in rice. However, its exact role in meiosis, especially as kinesin involved in homologous pairing and synapsis, has not been fully elucidated. Here, we generated three pss1 mutants by genome editing technology to dissect PSS1 biological functions in meiosis. The pss1 mutants exhibit alterations in the radial microtubule organization at pachytene and manifest a deficiency in telomere clustering, which is critical for full-length homologous pairing. We reveal that PSS1 serves as a key mediator between chromosomes and cytoskeleton, thereby regulating microtubule organization and transmitting the force to nuclei to facilitate homologous chromosome pairing and synapsis in meiosis.

Temperature change regulates pollen fertility of a PTGMS rice line PA64S by modulating the ROS homeostasis and PCD within the tapetum.

Photoperiod and temperature-sensitive male sterility rice is an important line for two-line hybrid rice, and the changes in the cultivation temperature strictly control its pollen fertility. However, the mechanism by which temperature variation regulates pollen fertility is still unclear. This study obtained stable fertile PA64S(F) and sterile PA64S(S) rice from PA64S by controlling temperature changes. PA64S(F) shows a normal anther development and fertile pollen under low temperature (21°C), and PA64S(S) shows delayed degradation of the tapetum cells, leading to abnormal pollen wall formation and ubisch development under normal temperature (28°C). The accumulation of reactive oxygen species (ROS) positively correlates with the programmed cell death (PCD) process of tapetum cells. The delayed accumulation of ROS in the PA64S(S) tapetum at early stages leads to a delayed initiation of the PCD process. Importantly, we localized ascorbic acid (ASA) accumulation in the tapetum cells and determined that ASA is a major antioxidant for ROS homeostasis. ROS-inhibited accumulation plants (PA64S-ASA) demonstrated pollen sterility, higher ASA and lower ROS accumulation in the tapetum, and the absence of PCD processes in the tapetum cell. Abnormal changes in the tapetum of PA64S(S) rice disrupted metabolic pathways such as lipid metabolism, cutin and wax synthesis, sugar accumulation, and phenylpropane, affecting pollen wall formation and substance accumulation, suggesting that the timely accumulation of ROS is critical for male fertility. This study highlights the central role of ROS homeostasis in fertility alteration and also provides an avenue to address the effect of environmental temperature changes on pollen fertility in rice.

A jasmonate-mediated regulatory network modulates diurnal floret opening time in rice.

Diurnal floret opening time (DFOT) is a pivotal trait for successful fertilization and hybrid breeding in rice. However, the molecular mechanism underlying this trait is poorly understood in rice. In this study, we combined the cytological, genetic and molecular studies to demonstrate that jasmonic acid (JA) regulates DFOT in rice through modulating the turgor and osmotic pressure of the lodicules. We show that lodicules undergo dramatic morphologic changes, accompanied by changes in water and sugar contents during the process of floret opening. Consistently, a large set of genes associated with cell osmolality and cell wall remodeling exhibits distinct expression profiles at different time points in our time-course transcriptomes of lodicules. Notably, a group of JA biosynthesis and signaling genes is continuously upregulated, accompanied by a gradual increase in JA accumulation as floret opening approaching. Furthermore, we demonstrate that the JA biosynthesis gene OsAOS1 is required for endogenous JA biosynthesis in lodicules and promoting rice DFOT. Moreover, OsMYC2, a master regulator of JA signaling, regulates rice DFOT by directly activating OsAOS1, OsSWEET4, OsPIP2;2 and OsXTH9. Collectively, our findings establish a core regulatory network mediated by JA for modulating rice DFOT and provide effective gene targets for the genetic improvement of DFOT in rice.

The characteristic analysis of TaTDF1 reveals its function related to male sterility in wheat (Triticum aestivum L.)

BackgroundThe male sterile lines are an important foundation for heterosis utilization in wheat (Triticum aestivum L.). Thereinto, pollen development is one of the indispensable processes of wheat reproductive development, and its fertility plays an important role in wheat heterosis utilization, and are usually influencing by genes. However, these key genes and their regulatory networks during pollen abortion are poorly understood in wheat.ResultsDEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 (TDF1) is a member of the R2R3-MYB family and has been shown to be essential for early tapetal layer development and pollen grain fertility in rice (Oryza sativa L.) and Arabidopsis thaliana. In order to clarify the function of TDF1 in wheat anthers development, we used OsTDF1 gene as a reference sequence and homologous cloned wheat TaTDF1 gene. TaTDF1 is localized in the nucleus. The average bolting time of Arabidopsis thaliana overexpressed strain (TaTDF1-OE) was 33 d, and its anther could be colored normally by Alexander staining solution, showing red. The dominant Mosaic suppression silence-line (TaTDF1-EAR) was blue-green in color, and the anthers were shrimpy and thin. The TaTDF1 interacting protein (TaMAP65) was confirmed using Yeast Two-Hybrid Assay (Y2H) and Bimolecular-Fluorescence Complementation (BiFC) experiments. The results showed that downregulated expression of TaTDF1 and TaMAP65 could cause anthers to be smaller and shrunken, leading to pollen abortion in TaTDF1 wheat plants induced by virus-induced gene-silencing technology. The expression pattern of TaTDF1 was influenced by TaMAP65.ConclusionsThus, systematically revealing the regulatory mechanism of wheat TaTDF1 during anther and pollen grain development may provide new information on the molecular mechanism of pollen abortion in wheat.

Lectin Receptor-Like Protein Kinase OsNRFG6 is Required for Embryo Sac Development and Fertilization in Neo-Tetraploid Rice

Great yield-enhancing prospects of autotetraploid rice was restricted by various polyploidy-induced reproductive dysfunction. To surmount these challenges, our group has generated a series of valuable fertile tetraploid lines (denoted as neo-tetraploid rice) through 20-year efforts. With this context, a G-type lectin receptor-like kinase, OsNRFG6, was identified as a pivotal factor associated with reproductive regulation in neo-tetraploid rice. Nevertheless, it is still elusive about a comprehensive understanding of its precise functional roles and underlying molecular mechanisms during reproduction of neo-tetraploid rice. Here, we demonstrated that OsNRFG6 executed a constitutive expression pattern and encoded proteins localizing in perinucleus and endoplasmic reticulum. Subsequently, four independent mutant lines of OsNRFG6 within neo-tetraploid rice background were further identified, all displaying low seed-setting rate due to abortive embryo sacs and defective double fertilization. RNA-seq and RT-qPCR revealed a significant down-regulation of OsNRFG6 and female reproductive genes such as OsMEL1 and LOG in ovaries prior to and post-fertilization, attributing this effect to OsNRFG6 mutation. Furthermore, through yeast-two hybrids, bimolecular fluorescence complementation assays, and luciferase complementation imaging assays, it was determined that OsNRFG6 could interact with itself and two female reproductive proteins (LOG and OsDES1) to form protein complexes. These results elucidate the reproductive functions and molecular pathway governed by OsNRFG6 in regulating fertility of neo-tetraploid rice, offering insights into molecular understanding of fertility improvement in polyploid rice.

A Quadruple Mutant of OsPRP1 Controls Pollen Fertility by Regulating the Expression of Anther Development-Related Genes in Oryza sativa L.

Plant proline-rich proteins (PRPs) are cell wall proteins that are widely distributed in plants. Previous studies have shown that these proteins play a crucial role in adversity stress processes, but their function in the regulation of pollen fertility in rice remains unknown. In this study, we identified that OsPRP1 contains a Pollen_Ole-e_I allergenic structural domain, obtained the OsPRP1 quadruple mutant named osprp1.1/1.2/1.3/1.4, and observed significant reductions in pollen fertility, seed-setting rates, and the deformation and collapse of microspores during the late stages of pollen development. RNA-Seq analysis indicated the down-regulation of genes involved in anther development in osprp1.1/1.2/1.3/1.4, suggesting that OsPRP1 plays a role in regulating pollen fertility. In conclusion, a loss of function in OsPRP1.1/1.2/1.3/1.4 leads to decreased pollen fertility and seeding rates, which not only expands the functional spectrum of plant PRP genes but also provides new theoretical insight into the mechanism of fertility regulation in rice.

Research on fertilization decision method for rice tillering stage based on the coupling of UAV hyperspectral remote sensing and WOFOST.

The use of chemical fertilizers in rice field management directly affects rice yield. Traditional rice cultivation often relies on the experience of farmers to develop fertilization plans, which cannot be adjusted according to the fertilizer requirements of rice. At present, agricultural drones are widely used for early monitoring of rice, but due to their lack of rationality, they cannot directly guide fertilization. How to accurately apply nitrogen fertilizer during the tillering stage to stabilize rice yield is an urgent problem to be solved in the current large-scale rice production process. WOFOST is a highly mechanistic crop growth model that can effectively simulate the effects of fertilization on rice growth and development. However, due to its lack of spatial heterogeneity, its ability to simulate crop growth at the field level is weak. This study is based on UAV remote sensing to obtain hyperspectral data of rice canopy and assimilation with the WOFOST crop growth model, to study the decision-making method of nitrogen fertilizer application during the rice tillering stage. Extracting hyperspectral features of rice canopy using Continuous Projection Algorithm and constructing a hyperspectral inversion model for rice biomass based on Extreme Learning Machine. By using two data assimilation methods, Ensemble Kalman Filter and Four-Dimensional Variational, the inverted biomass of the rice biomass hyperspectral inversion model and the localized WOFOST crop growth model were assimilated, and the simulation results of the WOFOST model were corrected. With the average yield as the goal, use the WOFOST model to formulate fertilization decisions and create a fertilization prescription map to achieve precise fertilization during the tillering stage of rice. The research results indicate that the training set R2 and RMSE of the rice biomass hyperspectral inversion model are 0.953 and 0.076, respectively, while the testing set R2 and RMSE are 0.914 and 0.110, respectively. When obtaining the same yield, the fertilization strategy based on the ENKF assimilation method applied less fertilizer, reducing 5.9% compared to the standard fertilization scheme. This study enhances the rationality of unmanned aerial vehicle remote sensing machines through data assimilation, providing a new theoretical basis for the decision-making of rice fertilization.

Bulked Segregant RNA-Seq Analysis of Pollinated Pistils Reveals Genes Influencing Spikelet Fertility in Rice

Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F5:6 generation plants derived from inter-subspecific crosses (Nipponbare × KDML105) with low (LS) and high seed-setting rates (HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4‒5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes (DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS- specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene (CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall (OsPME35 and OsPLL9) and controlling later processes such as pollen-stigma adhesion (Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.

Effectiveness of Using Liquid Organic Fertilizer Rice Wash and Coffee Grounds to Increase the Growth of Tomato Plants (Allium ascalonicum L.)

Tomato plants (Allium ascalonicum L.) are horticultural vegetable plants that are included in the eggplant group. Tomato plants are very much needed in the community because tomatoes are one of the most important vegetable ingredients in several types of vegetables. This study observed the growth of tomato plants under the influence of POC treatment of Rice Water and Coffee Grounds Waste. The method used in this study was a non-factorial Randomized Block Design (RAK) with three replications to see how the POC of rice washing water and coffee grounds waste affected the growth of tomato plants ( Allium ascalonicum L. ). With three types of treatment concentrations 0 (control), 100, 200ml POC and 0 (control), 75g, and 150g coffee grounds waste. The results of this research show that plants with a dose of 200ml POC have a high growth rate compared to other doses. With the same plant age, plant stem height occurred at a dose of 200ml, while the lowest number of leaves occurred at a dose of 0. This study found that administering a 200ml POC dose at 3MST showed a significant dose level, compared to other dose treatments. The results of this research show that a dose of 200ml POC of rice washing water is very suitable for the growth process of tomato plants, and the findings of this research can be used as a condition for getting tomato plant growth results.