The determination of the maturity of compost and other organic fertilizers is very important because of possible phytotoxic or phytostimulating effects. The maturity of compost can be assessed on the basis of chemical analyses, and a germination test with different test plants is most often used to determine phytotoxicity. In this research, the maturity of compost produced from the plant residues subsequent to the maintenance of green public areas was assessed using the results of chemical analyses. Simultaneously, a germination test was carried out with the four test plant species (cucumber, garden cress, triticale, and barley) to determine the phytotoxicity of compost extract in a ratio of 1:2.5 v/v (1:3.3 w/v) and 1:10 v/v (1:13.3 w/v) and the three ammonium N solutions (in the concentrations of 200, 400, and 600 mg/L NH4-N). According to the chemical properties of compost (primarily the C/N, NH4-N/NO3-N ratios, and the NH4-N concentration) and the germination test with cucumber and garden cress, we may conclude that the tested compost was mature and that we did not expect a phytotoxic effect. The choice of a plant is very significant because the germination test with a compost extract demonstrated an undoubted phytostimulating effect on the garden cress and cucumber, with a more pronounced phytostimulating effect of the 1:10 than that of the 1:2.5 v/v compost extract. No such effect was detected on the monocotyledonous test plants triticale and barley since the 1:10 v/v extract had no significant effect, and the 1:2.5 v/v extract had a phytotoxic effect, moderate on the triticale and high on barley. The conclusion is that garden cress and cucumber are suitable test plants for the determination of compost’s phytostimulative effect, but they are not suitable for the determination of phytotoxicity for monocotyledonous plants, especially if the cause of phytotoxicity is a non-ammonium component. Barley is the most suitable species for the determination of compost’s non-ammonium phytotoxicity and nitrogen’s ammonium-form phytostimulative or phytotoxic effect. It would be very useful to conduct a comparative germination test with the compost extracts in the ratios 1:2.5 and 1:10, whereby the 1:2.5 extract would be used as a test of phytotoxicity, and the 1:10 extract for the test of a phytostimulating effect.
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