Fertility Problems Research Articles

The degree of toxoplasmosis and testicular histomorphometry in rats.

Toxoplasma gondii (T. gondii) ranks as the third most common parasitic parasite worldwide, and it is estimated that > 60% of the population is infected with T. gondii worldwide at some point in their lives. So. Therefore, it is highly curious that T. gondii could be a potential cause of idiopathic infertility that is incidental to male partners, who are responsible for nearly 50% of cases. Testicular histomorphometric analysis was developed to investigate fertility problems. The objective of this experimental study was to assess the impact of toxoplasmosis on spermatogenesis indicated by histomorphometric changes in rat testes and its correlation with the degree of infection in the brain. Ninety male Wistar albino rats were infected with T. gondii, and 30 male rats composed the control group. The studied parameters were investigated from the seventh week until the twelfth week postinfection by estimating the body weight, the weight of the testes, histopathological examination, and metric analysis of the testes. Each time, correlations were detected between the investigated parameters and the infection severity calculated by estimating the cyst burden in brain homogenates and brain lesion grading of stained histological sections.Our findings demonstrated a significant adverse impact of toxoplasmosis on absolute body weight, testis weight, and testis histomorphometry. The grading of brain lesions and the number of brain cysts paralleled each other. There was a reverse correlation between the gonado-somatic index and the number of brain cysts and brain lesion grade. There was a statistically significant correlation between the brain cyst count and the brain lesion grade and the indices 20A, 20b, 250, and 200 of the testes metric analysis. Conclusion: Our results revealed that toxoplasmosis has an adverse impact on male rat spermatogenic cells, which in turn affects spermatogenesis and fertility. This impact is significantly correlated with the degree of latent infection in the brain.

The effectiveness of multimedia mental health self-care program based on cyber space on the mental health of infertile women: a randomized controlled trial.

So far, some training interventions have been carried out to improve the mental health in women with infertility, but designing the need and evidence-based, as well as multimedia mental health self-care interventions based on cyber space has received less attention. Due to the spread of the internet and the role of self-care in improving mental disorders, this study was conducted to evaluate the effectiveness of the multimedia mental health self-care program on mental health and to assess the users' satisfaction. This study is a randomized controlled trial with pretest-posttest follow-up design. The sample was selected using a convenience sampling method (n = 90). The random number function was used to assign random numbers. The research instruments include a demographic, psychological Well-being, depression, anxiety, perceived stress, fertility problems and satisfaction with training questionnaire. Six weeks of intervention was conducted following the pre-test and the link of each session's content was sent to the participants, based on the training schedule, through Eitaa Messenger. The post-test and follow-up were conducted 1week and 1month post intervention. The data were analyzed using independent t-test and repeated measures ANOVA. A statistically significant difference was observed between the intervention and control group in the mean score of psychological well-being, perceived stress and infertility stress 1week and 1month post intervention and in the mean score of depression and anxiety 1month post intervention. The intervention group scored higher than the control in psychological well-being but lower in perceived stress, depression, anxiety and infertility stress. The intervention had a positive effect and reduced the score of perceived stress, depression, anxiety and infertility in the intervention group over time. The score reduction continued until the follow-up stage. No significant time-interaction effect was observed on psychological well-being and on the control group. Satisfaction with the program and subscales was desirable. This program could significantly reduce the depression, anxiety, perceived stress and infertility stress and desirable satisfaction with the program was observed among users. This program can be used in designing the experimental and therapeutic interventions to improve mental-health self-care behaviors. RCT Registry: Iranian Registry of Clinical Trials; RCT registration number: IRCT20210526051410N1; Registration date: 2022-11-06. Last update: 2023-01-28 https://irct.behdasht.gov.ir/trial/57874.

THE PROGINS VARIANT OF THE PGR GENE AND PLACENTAL ENDOCRINE FUNCTION AT RISK OF PRETERM LABOR: A PILOT STUDY

Progesterone receptors, which are encoded by the PGR gene, mediate the main physiological effects of progesterone – the hormone that is required for a successful pregnancy. The PROGINS variant is one of the most common variants of the PGR gene. Due to genetic heterogeneity, the presence of inferior forms of progesterone receptors in women can be a clinical problem. In addition to fertility problems, such women may theoretically respond differently to progesterone replacement therapy.Aim of the study to determine the effect of the PROGINS variant of the PGR gene on the hormonal status and results of progesterone deficiency treatment in pregnant women at risk of preterm labor.Material and methods. The main group included 30 pregnant women with progesterone deficiency and the threat of preterm labor, and the comparison group included 30 pregnant women with a normal course of pregnancy. The concentrations of progesterone, estradiol, and placental lactogen were measured in the pregnant women of both groups. Genotyping was assayed by PCR.Results. The genotypic frequencies of the PROGINS variant were not significantlydifferent between the main and comparison groups. Pregnant patients in the main group with the T2T2 genotype after treatment had significantly lower progesterone levels compared to patients with the T1T1 and T1T2 genotypes. Pregnant women in the main group with the T2T2 genotype delivered at a shorter gestational age.Conclusion. The results of our study showed the associations between the PROGINS variant of the PGR gene and the inefficacy of progesterone deficiency treatment in pregnant women at risk of preterm birth and shortened gestation.

Male infertile couples: an actor-partner interdependence model of attachment styles and fertility pressure

BackgroundCouples undergoing artificial insemination by donor semen(AID) represent a unique group of infertility patients, the relationship between fertility pressure and attachment styles remains unclear. To investigate the fertility pressure and attachment dimensions in artificial insemination by donor semen(AID) couples, and develop an Actor-Partner Interdependence Model (APIM) for patient care.MethodsThis study is a cross-sectional study. From June to September 2021, couples who were about to receive AID treatment in our hospital were assessed using the Fertility Problem Inventory, and the Experiences in Close Relationships(ECR). Dyadic analysis applying the APIM was used.ResultsA total of 448 valid questionnaires were recovered, the effective response rate was 94.9%. The average score of men’s fertility pressure was higher than that of women’s(P = 0.021). The score of male attachment anxiety was higher than that of females(P = 0.038). Attachment avoidance(β = 0.46) and attachment anxiety(β = 0.33) in wives significantly impacted their fertility pressure. Attachment avoidance(β = 0.17) and attachment anxiety(β = 0.52) in husbands significantly impacted their fertility pressure. The attachment avoidance of wives’ had a significant impact on the husbands’ fertility pressure(β = 0.11).ConclusionOverall, the study revealed that in the couples who use donor sperm to expect pregnancy, the male has higher fertility pressure. Insecure attachment can cause more severe fertility pressure, fertility pressure is also affected by one’s partner.

Influence of biofertilizers on potato (Solanum tuberosum L.) growth and physiological modulations for water and fertilizers managing

Biofertilizers constitute a safe way to alleviate the impacts of water shortage and the overload of chemical fertilizers. This work focuses on the effects of Arbuscular Mycorrhizal Fungi (AMF) and Plant Growth-Promoting Rhizobacteria (PGPR) on potato's agro-physiological response, beneath the reduction of water, nitrogen (N), phosphorous (P) and potassium (K) fertilizers. Five microbioal treatments (C: Control, AMF: Mycorhizae, B1: Bacillus halotolerans, B2: Bacillus amyloliquefaciens and B1B2: B.halotolerans+ B.amyloliquefaciens) were tested in arrangement with two nitrogen-phosphorous-potassium (NPK) rates (F1: Entire rate, F ½: Half rate) and three irrigation levels (100 % of crop water requirement: 100 % ETc; 75 % ETc; 50 % ETc) in a two year factorial split plot design. We studied vegetative growth, photosynthetic activity, chlorophyll fluorescence, water status, osmoticums, N, P, K and enzymatic antioxidants accumulation. Data obtained in 2019–2022 showed that AMF and B1B2 biofertilizers were beneficial to improve Root/Shoot, Dry Underground Part/Aerial Part, leaf area (LA), water saturation deficit (WSD), chlorophyll index (SPAD) and tuber yield under 75 % ETc. The photosynthetic promoter effect of biofertilizers under 75 % ETc, compared to 50 % ETc was observed in the following trend B1B2>AMF>B1. The efficacy of B1B2 and AMF were coupled to high catalase (CAT) activity. Potatoes treated with B1B2 were found to be efficient for 75 % ETc in terms of proline accumulation. The AMF was more effective in terms of soluble sugars (SS) accumulation and control of osmotic potential (Ψs). Taking together, the B1B2 and AMF could be regarded as efficient biofertilizers under 75 % ETc, alleviating the impacts of water stress and the problem of excessive fertilization in potatoes.

Study of effect of Brassica oleracea L. var. italic on fertility in normal mice and rats model

Infertility is a widespread issue that has profound psychological, economic, demographic, and medical effects. While broccoli is a nutritious vegetable packed with vitamins, minerals, and antioxidants, its specific role in solving fertility problems is not well-proven.To investigate the fertility-enhancing properties of the hydroalcoholic extract derived from Brassica oleracea L. var. italic (BOLVI) in rat and mice model. In this study, the extract underwent phytochemical screening, and an acute oral toxicity assessment was following OECD guideline 423. Both, wistar rats and albino mice were employed as study subjects.The research included an analysis of the effects of BOLVI on ovulation in rats and a histopathological investigation. In the mice model, litter counts and sexual behaviors were recorded. It was noted that the hydroalcoholic extract of BOLVI, administered at a dose of 800 mg kg−1, yielded the highest ovulation rate, reaching 99.71±0.166%. Additionally, in the groups treated with BOLVI at doses of 200, 400, and 800 mg kg−1, histopathological examination indicated an increasing number of ovulation sites, with two, three, and four sites observed, respectively. Furthermore, the study demonstrated that the hydroalcoholic extract of BOLVI at a dose of 800 mg kg−1 significantly enhanced litter count and sexual behavior. The present phytoconstituents have been shown to possess fertility activity, deceased oxidative stress and increase in sperm count, sperm motility and sperm viability.

Confidence in Pregnancy Among U.S. Women

Researchers often ask women about pregnancy intentions but seldom ask how confident women are that they will be able to become pregnant when they desire. We argue that beliefs about fertility and infertility may function as justifications for behaviors to which one is already committed. Thus, women who have delayed childbearing but who intend to become pregnant may express confidence in their ability to become pregnant, even in the face of diminished odds of conception. Using data from the National Survey of Fertility Barriers, we demonstrate that over 75 percent of women who intend a first or subsequent pregnancy express confidence that they will be able to become pregnant when they wish, even if they perceive themselves as having a fertility problem or meet medical criteria for infertility. Our results are consistent with the motivated reasoning perspective. Our findings suggest that confidence in pregnancy may be a response to social‐structural pressures to delay childbearing among women who intend to become pregnant, but not necessarily the reason for delay.

In vitro maturation of oocytes: what is already known?

Assisted reproductive technologies (ARTs) involve the laboratory manipulation of gametes and embryos to help couples with fertility problems become pregnant. One of these procedures controlled ovarian stimulation uses pharmacological agents to induce ovarian and follicular maturation in vivo. Despite the effectiveness in achieving pregnancy and live births, some patients may have complications due to over-response to gonadotropins and develop ovarian hyperstimulation syndrome (OHSS). In vitro maturation of oocytes (IVM) has emerged as a technique to reduce the risk of OHSS, particularly in women with polycystic ovary syndrome (PCOS), and for fertility preservation in women undergoing oncological treatment. Although there are some limitations, primarily due to oocyte quality, recent advances have improved pregnancy success rates and neonatal and infant outcomes. Different terms have been coined to describe variations of IVM, and the technique has evolved with the introduction of hormones to optimize results. This review we provide a comprehensive overview of IVM and its reproductive outcomes during ARTs.

Suspected Cases of Chlamydia-Induced Fertility Problems in Sows: What Is the Approach of Austrian Practitioners?

Although it is generally accepted that Chlamydia spp. can induce fertility problems in sows, many questions concerning diagnostic, therapeutic, and prophylactic approaches remain unanswered. The aim of this study was to gather information about how swine practitioners deal with these knowledge gaps. Austrian swine practitioners were anonymously asked for their assessment of chlamydiosis using the Lime Survey program. PCR tests are used for molecular chlamydia detection from abortion material (64.3%), vaginal swabs (40.5%), and the genital tract (postmortem slaughterhouse samples) (9.5%). Treatment mainly includes tetracyclines (95.2%), occasionally macrolides (2.4%) or other (not mentioned) antibiotic groups (7.1%). Treatment periods vary greatly, from 5 to 10 days (23.8%), from 11 to 15 days (47.6%), from 16 to 21 days (11.9%), and >21 days (9.5%). Prophylactic measures include hygiene improvement, rodent control, and herd treatments with tetracyclines. Serological methods are perceived as inadequate, and there is a general need for better guidance on interpreting results. Knowledge about the increased incidence of tetracycline-resistant Chlamydia suis strains is limited. Weighing up economic interests, feasibility, and animal welfare plays an important role. Further research to improve diagnosis and prevention of chlamydia-related fertility diseases in sows is urgently needed.

Fertilizer Micro-dosing: Evaluating the Yield Response of Sorghum to Different Levels of Fertilizers Applied on the Planting Pit at Mereb-Lekhe District

Next to drought, poor soil fertility is the single major cause of crops nutrient starvation in Tigray specifically at Mereb-lekhe district. Farmers of the study area use different organic and inorganic fertilizers separately and in combination to overcome soil fertility problems for sorghum production. However, the pace of sorghum production stays constant and occasionally decreases with time. Fertilizer efficiency; the type, rate and method of fertilizer application they use were thought to be the reasons for the steady or declining sorghum production. Hence, this study was initiated to determine the yield response of sorghum to microdose fertilizer application. The experiment was set in a randomized complete block design with three replications. The treatments were; farmers’ practice or blanket recommended nitrogen (N) and phosphorous (P) (50kg ha-1 Urea & 100 kg ha-1 DAP), recommended compost at a rate of 7ton ha-1, 75% of recommended NP, 50% of recommended NP, 25% of recommended NP, 75% recommended NP+25% recommended compost, 50% recommended NP+50% recommended compost and 25% recommended NP+75% recommended compost. All treatments were applied with microdosing method except the recommended N and P is broadcasting method. The highest sorghum grain yield (4201 kg ha-1) and straw yield (19107 kg ha-1) were obtained from treatment recommended compost at a rate of 7 ton/ha and 50% recommended NP+50% recommended compost, respectively. This study showed that combined application of organic and inorganic fertilizer gave a better economic advantage for sorghum crop. Hence, it could be concluded that use of compost at a rate of 7 ton ha-1 and the 75% recommended N and P could increase production and productivity of sorghum by the application of micro dosing than broadcasting method.

7452 A Diagnostically Challenging Case of Hyperandrogenism in Postmenopausal Woman

Abstract Disclosure: M.S. Hossain: None. S. Hossain: None. B. Gautam: None. H. liao: None. S.C. Kumar: None. D.S. Rosenthal: None. Background: The diagnosis of new-onset severe hyperandrogenism in postmenopausal women is rarely encountered, posing a unique challenge for clinicians. Ovarian hyperthecosis and androgen-secreting tumors are the usual suspects in post menopause. In our case ovarian hyperthecosis is the primary consideration but elevated 17-OH Progesterone (17-OHP) introduces the potential co-existence of Non classical congenial adrenal hyperplasia (NCCAH) or rare instances of Sertoli-Leydig Cell Tumor (SLCT). Case Presentation: A 70 years old female, with no past medical history, referred to the Endocrinology clinic for significantly elevated testosterone level. She reported excessive hair growth in various areas of her body since 2022, which required shaving almost every day. She also reported male pattern hair loss and weight loss of 15 lbs over the last three months. She denied headache, vision changes, deepening of voice, history of fertility problems and use of exogenous testosterone. Her vital sign were normal. On examination, she had clitoromegaly, terminal hair growth on her upper lip and chin, breast atrophy, male pattern baldness, but no cushingoid or acromegaly features. Blood tests showed significantly elevated total testosterone: 1422 ng/dl (2-45); free testosterone: 147.9 pg/ml (0.2-3.7) and Estradiol: 64 pg/ml (Postmenopausal <31) with low LH: 7.6 mIU/ml (Postmenopausal 10.0-54.7) and FSH: 22.2 mIU/mL (Postmenopausal 23-116). Her serum ACTH, DHEA Sulfate, 11-deoxycortisol, 17 OH pregnenolone, TSH, Free T4, prolactin, IGF-1 were normal while her 17-OHP: 396 ng/dl (<45 ng/dl) was significantly elevated. An ACTH stimulation test showed 17-OHP level of 734 ng/dl at 30 minutes and 901 ng/dl at 1 hour. Pelvic ultrasonography: right ovary of 2.3 x 1.6 x 1.9 cm and the left ovary of 3.1 x 1.8 x 3.0 cm. MRI showed normal adrenal glands but a prominent left ovary. Patient was referred to the Obstetrics department to undergo bilateral oophorectomy for the definitive diagnosis and treatment. Discussion: The current ACTH stimulation test for non-classical congenital adrenal hyperplasia is validated for premenopausal women but lacks standardization in post-menopausal state. In our case, distinguishing the source of elevated 17-OHP between adrenal (NCCAH) and ovarian (SLCT) was difficult. Unexpected asymmetry in ovarian size observed in our case further complicates the scenario, contrasting with the typical symmetrical enlargement seen in ovarian hyperthecosis. Pending surgical pathology will further guide the management. This case highlights the complexity of diagnosing hyperandrogenism in postmenopausal women, emphasizing the significance of thoroughly exploring differential diagnoses and meticulously analyzing laboratory results. Presentation: 6/2/2024

Deep endometriosis – impact on infertility, endometriosis fertility index, and reproductive prognosis (comparative study)

Introduction Deep infiltrating endometriosis (DIE) is the most severe form of endometriosis, contributes to pelvic pain syndrome, extragenital symptoms, fertility problems, and diminished reproductive prognosis for affected individuals. It is recommended to use the Endometriosis Fertility Index (EFI) to assess reproductive prognosis and conduct clinical research to compare reproductive prognosis in different forms of endometriosis. Objective Comparison of the impact of the DIE and others forms of endometriosis on EFI, patients' fertility and on reproductive prognosis to understand the management approach. Materials and methods A cohort study included 190 reproductive-age women, divided according to the #Enzian classification: the main group - 85 patients with DIE, the control group - 105 women with other forms of endometriosis. The EFI was utilized for reproductive prognosis. Pain was evaluated with Visual Analog Scale (VAS). Statistical analyses were performed using SPSS, with calculation of the Mann-Whitney U and Pearson's chi-square test (χ²). Results In main group, the frequency of infertility was 83.5% compared to the control group's 71.4% (p > 0.05). The EFI in main group was 7.18±0.25 points vs the control group's 7.13±0.28 points (p = 0.852). Patients in the main group suffered from intense pelvic pain (>7 points by the VAS, p < 0.01), including severe dyspareunia (7.85±0.33 points vs 2.18±0.46 points in control group, p < 0.01). Conclusions Our results suggest that infertility in women with DIE may be more often associated with sexual abstinence due to significant dyspareunia rather than organic impairments. Thus, EFI in patients with DIE does not reflect all aspects of infertility and has reservations, and consideration of both physical symptoms and sexual health is crucial in managing DIE to optimize fertility outcomes. These findings open the way to the feasibility of surgical treatment of DIE to improve sexual quality of life, which will reduce the need for IVF and increase the chances of spontaneous pregnancy in patients, but this conclusion requires further investigation in randomized clinical trials.

Psoriasis in women with psoriatic arthritis: hormonal effects, fertility, and considerations for management at different stages of life.

This review examines skin manifestations in women with spondyloarthritis, with a particular focus on psoriatic arthritis (PsA) and associated psoriasis. A narrative review of the bibliography was conducted using the main databases (PubMed, Scopus, EMBASE). The review showed that the clinical course of PsA and psoriasis in women is influenced by hormonal fluctuations that occur at different stages of life, such as menstruation, pregnancy, postpartum, and menopause. Gender differences in the epidemiology of PsA and psoriasis are discussed and attributed to biological, hormonal, and environmental differences. The role of estrogen in modulating immune responses and its impact on the severity of PsA and psoriasis are reviewed. Special emphasis is placed on the psychosocial impact of visible skin lesions on women's quality of life and fertility problems associated with psoriasis. Treatment strategies are also taken into account, favoring personalized approaches that consider the safety of treatments during pregnancy and breastfeeding. The review highlights the importance of a holistic and gender-sensitive approach to the management of PsA and psoriasis in women, promoting the integration of physical treatment with support for emotional well-being.

Genetic determinants of age at menarche: does the LIN28B gene play a role? A narrative review.

Menarche, the first menstrual period marking the onset of female reproduction, is a milestone of female puberty. The timing of menarche determines the timing of later phases of pubertal maturation in girls and has major implications for health later in life, including behavioral and psychosocial disorders during adolescence and fertility problems and increased risk for certain diseases in adulthood. Over the last few decades, a continuous decline in age at menarche has been noted, with environmental factors contributing to this change in the timing of menarche. However, a genetic component of age at menarche and pubertal onset has been strongly suggested by studies in families and twins wherein up to approximately 80% of the variance in puberty onset can be explained by heritability. Gene association studies have revealed several genetic loci involved in age at menarche, among which LIN28B has emerged as a key regulator of female growth and puberty. LIN28B, a human homolog of Lin28 of C. elegans, is a known RNA-binding protein that regulates let-7 microRNA biogenesis. Genome-wide association studies have identified the association of polymorphisms in the LIN28B gene with age at menarche in several population cohorts worldwide. In this paper, we review the genetic factors contributing to age of menarche, with particular focus on the identified polymorphisms in LIN28B gene.

The Effect of COVID-19 and COVID-19 Vaccination on Assisted Human Reproduction Outcomes: A Systematic Review and Meta-Analysis.

The most discussed infectious disease is coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. Many research endeavors have focused on the effects of the virus on reproductive organs, as these have also been shown to carry the receptors to which the virus attaches. The results of assisted reproductive technology (ART) have been significantly affected by the pandemic, with some in vitro fertilization (IVF) centers being closed due to the risk of further spread of the disease. According to World Health Organization statistics, 17.5% of adults worldwide suffered from fertility problems in 2023; in other words, one in six people in the world have reproductive health problems. As infertility is a growing problem in the modern world and new developments in assisted reproduction are always a topic of profound interest, it is important to understand the impact of SARS-CoV-2 on reproductive health. This systematic review aimed to examine studies describing patients undergoing ART procedures with a COVID-19-positive history and to shed light on the recent evidence on the safety of COVID-19 vaccination in the ART context. A meta-analysis was conducted to confirm the results of the systematic review. The results showed a significant difference in clinical pregnancy rates between the vaccinated and unvaccinated groups and an increased miscarriage rate in those with a COVID-19-positive history. However, no significant difference in clinical pregnancy and birth rates was found in participants with a previous COVID-19 infection. The results show that further studies and research are needed, even though the spread and impact of the virus have decreased. Evidence-based information for individuals and couples undergoing infertility treatment is vital to enable informed decision-making.

Preconception carrier screening in couples seeking in vitro fertilization: exploring the patient perspective

Research questionWhat is the level of understanding, and what are the attitudes and considerations regarding preconception carrier screening (PCS) among patients seeking in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI)? DesignA mixed methods design was used. Nine interviews were conducted with couples or individual partners (N = 16) who had an intake for IVF/ICSI in the two years prior to this study. A questionnaire was completed by 115 participants. No actual PCS was offered. ResultsAll interviewed couples expressed a positive attitude towards PCS, and over half of the respondents would pursue or seriously consider pursuing PCS if possible. Some couples falsely believed that PCS could identify a cause for their fertility problems and increase their chances of conceiving. The desire to make an informed reproductive decision was the most important argument in favour of PCS. The primary argument against PCS was the apprehension of being confronted with reproductive dilemmas. The longer the delay in their IVF/ICSI treatment required to perform PCS, the more couples would be inclined to decline screening. Participants preferred receiving information about PCS from a medical specialist at an early stage in their IVF/ICSI treatment. ConclusionAlthough attitudes towards PCS were generally positive, some concerns were raised about treatment delays and potential reproductive dilemmas, and some couples had misconceptions about the purpose of screening within the context of their IVF/ICSI treatment. These findings highlight the importance of tailoring the information and counseling to the specific needs of IVF/ICSI patients.

Fertility Stress, Psychological Resilience, and Depressive Symptoms in Women With Polycystic Ovary Syndrome.

Infertility is a common problem for patients with polycystic ovary syndrome (PCOS), which is closely related to stress and raises the risk of depression, while psychological resilience has been revealed to be protective for mental health. However, the associations of fertility stress, psychological resilience, and depressive symptoms in individuals with PCOS are not thoroughly explored. Our study aims to examine the associations of fertility stress and psychological resilience with depressive symptoms among patients with PCOS, as well as the mediating role of psychological resilience. A cross-sectional study was conducted in a reproductive clinic. The participants completed structured questionnaires on fertility stress (Fertility Problem Inventory (FPI)), psychological resilience (10-Item Connor-Davidson Resilience Scale (CD-RISC 10)), and depressive symptoms (Beck Depression Inventory-II (BDI-II)). Hierarchical regression was utilized to explore the relationship between fertility stress, psychological resilience, and depressive symptoms in PCOS patients after controlling for covariates. Psychological resilience was included in the final layer of the regression model to determine its potential mediating roles by comparing changes in the effect sizes between models. The percentage of mediating effect was then determined using structural equation modeling (SEM). A total of 192 women with PCOS were included. Of them, 50 (26%) presented depressive symptoms, 18% of which were severe. Hierarchical regression showed that after adjusting for sociodemographic and health-related characteristics, both fertility stress (β=0.361; p<0.001) and resilience (β=-0.453; p<0.001) were significantly correlated with depressive symptoms. SEM demonstrated that fertility stress was directly linked to depressive symptoms in women with PCOS (β=0.050; 95% CI (0.028, 0.084); p<0.001). Psychological resilience mediated 21.9% of the relationship between fertility stress and depressive symptoms (β=0.014; 95% CI (0.005, 0.034); p=0.001). This study demonstrated that among women with PCOS, fertility stress was, directly and indirectly through psychological resilience, associated with depressive symptoms. However, this study was cross-sectional, and the participants were recruited from a single clinical hospital. If replicated in longitudinal studies, the findings provide fertility stress and psychological resilience as potential intervention targets for better mental health in this population.

Development of a rapid test to determine endometritis of cows after calving.

Postpartum uterine disease, such as endometritis, is widespread in highly productive dairy cows, leading to fertility problems and economic losses. Despite existing diagnostic methods, early and effective detection of uterine infections remains problematic due to the subclinical nature of some conditions. This study aimed to develop and evaluate a rapid diagnostic test for endometritis in cows on different days postpartum (PP). The study was carried out on dairy Holstein-Friesian cows (n = 121) kept on farms in northern Kazakhstan. The study included both clinically normal cows and those diagnosed with endometritis, ensuring a comprehensive evaluation of the diagnostic methods across different stages of uterine health. The following laboratory tests were performed to diagnose and evaluate the presence and severity of endometritis in the cows: Nagorny-Kalinovsky test, Whiteside test, Katerinov test, Gavrish silver nitrate test, peroxide test, and clinical tests (rectal and vaginal examination). These tests were specifically chosen to identify inflammatory processes in the uterus, particularly focusing on detecting endometritis. From day 21 to 30 PP, rectal and vaginal examinations were 32% and 28% more effective than the Nagorny-Kalinovsky test and the Whiteside test. From day 61 postpartum, the Whiteside test was 37.5% more effective than the Nagorny-Kalinovsky test. Comparatively, among laboratory diagnostic methods from days 10 to 110 PP, the peroxide test showed the greatest effectiveness in identifying 80.9% of sick animals. In sick animals from day 10 to 20 PP, during the interaction of the cervicovaginal mucus with 10%, 20%, and 30% hydrogen peroxide (H2O2), an 8.1 ± 1.9-8.8 ± 1.6 cm foam column was formed within 4-5 min. The experiment showed that a 10% H2O2 solution yielded better results. Using H2O2 as a diagnostic agent for endometritis in cows has several advantages, including ease of use, it does not require special laboratory conditions and provides a visual reading of the reaction within 4-5 min. A limitation of this study is the focus on H2O2 without exploring other potential reagents that may enhance diagnostic accuracy. Future research could explore the long-term stability of cervicovaginal mucus samples and investigate the integration of additional substances that may expedite the detection of subclinical endometritis and improve the clarity of diagnostic results.

The quality and characteristics of bovine sperm are compromised by Toxoplasma gondii antigens, impacting in in vitro bull fertility

Studies in various species have demonstrated different results on the effects of T. gondii infection on sperm quality. It has also been demonstrated that in some stages of the disease, there is elimination of cellular debris or even the intact parasite in the semen. The present work aimed to evaluate the in vitro effects of the presence of soluble T. gondii antigens in bovine semen on sperm integrity. The spermatozoa were treated with T. gondii antigens in double serial dilutions classified as high, medium and low doses (8, 4, 2 µg/ml) in "TALP-Sperm” and “TALP-Fert" media. The results showed that T. gondii antigens affect sperm motility and mitochondrial activity, and cause changes in sperm chromatin integrity, as well as damage to the sperm membrane and acrosome. Finally, spermatozoa treated with T. gondii antigens were evaluated in the in vitro production of embryos (IVEP). The use of semen contaminated with antigens in IVEP routines did not lead to a decrease in the fertilization of oocytes, as sperm undergo selection before in vitro fertilization, which eliminates the most altered sperm. However, early embryonic development was affected, probably by structural changes that were not eliminated in the selection process. The results demonstrated that the presence of soluble T. gondii antigens in bovine semen alters sperm integrity and vital characteristics for the fertilization process and embryonic development and therefore causes fertility problems in males.

Resection of uterine arteriovenous fistula with temporary occlusion of the internal iliac arteries: Case series and literature review.

Uterine arteriovenous fistula (UAVF) is a rare vascular abnormality that can cause severe and potentially life-threatening hemorrhage. Uterine artery embolization is a common treatment that may affect ovarian and uterine perfusion and cause fertility problems. We herein report our experience treating 2 patients with UAVF who underwent resection after temporary occlusion of both internal iliac arteries. Both patients presented with a large UAVF after incomplete miscarriages in the second trimester. Magnetic resonance imaging revealed a UAVF measuring 3.6 × 2.6 × 2.1 cm over the myometrium of the posterior uterine in case 1, and a UAVF of 7.1 × 4.1 × 4.5 cm was identified in case 2. Uterine arteriovenous fistula, retained products of conception. The patients underwent resection of UAVF with temporary occlusion of the internal iliac arteries and hysteroscopic removal of the retained products of conception. Intraoperative bleeding were minimal. Neither patient exhibited abnormal uterine bleeding at the 6-month follow-up. Follow-up ultrasonography and magnetic resonance imaging showed normal uterine myometrium and endometrium and no residual disease. UAVF resection after temporary occlusion of the internal iliac arteries is a promising treatment approach for UAVF. This technique can reduce intraoperative bleeding and remove the potential hemorrhage-related lesion while preserving fertility.