Fertile Phase Of Menstrual Cycle Research Articles

Salivary Proteome Profile during the Fertile Phase of Menstrual Cycle: Correspondence.

Salivary Proteome Profile during the Fertile Phase of Menstrual Cycle: Correspondence.

Salivary Proteome Profile of Women during Fertile Phase of Menstrual Cycle as Characterized by Mass Spectrometry.

Objectives:Ovulation is such a critical physiological process that its noninvasive detection based on salivary constituents has several advantages in humans. Hence, the present study is proposed to identify the ovulatory-specific proteins in saliva in order to detect ovulation phase.Materials and Methods:Samples were collected from women volunteers. The procedure adopted was approved by the Institutional Human Ethical Committee (DM/2014/101/38), Bharathidasan University. The saliva samples were collected from thirty healthy female volunteers, with a prior written consent. One-way analysis of variance was used to calculate protein concentration and band intensity using SPSS 16 software (SPSS Inc., Cary, NC, USA). The salivary protein expression pattern during different phases of menstrual cycle was analyzed using gel-based high resolution-liquid chromatography-mass spectrometry/mass spectrometry and matrix-assisted laser desorption ionization-time of flight/time of flight. Further, bioinformatics tools were adopted to annotate the proteins identified at various phases of menstrual cycle.Results:As many as 530 proteins showed up in the saliva during ovulatory phase, whereas there were only 251 proteins identified during postovulatory phase. The functional annotation of salivary proteins revealed that the proteins got assigned to the class of “extracellular proteins” which are concerned with regulatory functions. The 16 unique and/or differentially expressed protein spots appeared during ovulatory phase, among which Cystatin-S, Prolactin-inducible protein, Cystatin-A, Cystatin-SN, BPI fold-containing family A member 2, Alpha-tubulin N-acetyltransferase 1, Carbonic anhydrase-6, Protein LEG1 homolog, Hemoglobin subunit beta, and Pancreatic alpha-amylase were identified.Conclusion:Total salivary proteome profile has been listed with respect to various phases of menstrual cycle. Among the protein listed, Cystatin-S offers a biomarker protein and/or indicator of ovulatory phase. However, extensive validation is required before arriving to a candidate bio-marker protein.

The New Fertility Testing Tape

To Editor:— InThe Journal, April 16, 1960, p. 744, Drs. Doyle, Evers, and Sapit report on The New Fertility Testing Tape, stating that a paper tape impregnated with glucose oxidase and a peroxidase will turn from pink to blue in presence of glucose. In cervical mucus, glucose is alleged to be derived partly from follicular fluid, although possibility of some glucose being derived from glycolysis within fundus and cervix is postulated. They recommend use of a special vaginal applicator to hold testing paper. If, after 3 minutes in vagina, it turns blue, woman is presumed to be in the fertile phase of menstrual cycle. Based on such assumptions, Fertility Testor Company recently placed large advertisements in Los Angeles Times insinuating that this technique would serve as an accurate method for contraception as well as conception,