Soybean Research Articles

Unveiling kinema: blending tradition and science in the Himalayan fermented soya delicacy

AbstractKinema, a sticky-textured fermented soybean food with umami flavour, is originated in the historical Limbuwan region, now situated in the eastern districts of Nepal. It has since expanded to different areas in Darjeeling Hills and Sikkim in India and Bhutan. The Limboo community, one of the oldest indigenous groups in the Himalayas, has developed a method for fermenting soybeans to produce kinema. Bacillus is the main bacterial genus responsible for fermenting kinema, along with lactic acid bacteria, yeasts, and fungi. Kinema is full of nutrients like protein, fats, carbohydrates, amino acids, vitamins, and minerals and offers health benefits through its antioxidant, anti-inflammatory, antiobesity, and antidiabetic properties. A novel strain of Bacillus subtilis Tamang has been identified in kinema samples from Darjeeling Hills, India, exhibiting elevated levels of γ-PGA and various biomarker genes for health benefits and biological functionalities. Kinema contains secondary metabolites including several bioactive compounds, immunomodulators, vitamin B-complexes, and others that help promote good health. The transcriptome of the B. subtilis Tamang strain reveals genes associated with GABA production, Vitamin B12 synthesis, and fibrinolytic activity. Safety evaluations have demonstrated that the strain and the production of kinema do not pose any toxicity risks based on animal studies. Despite facing challenges in terms of its strong taste and slimy consistency for broader acceptance, the bioactive elements and safe starter cultures found in kinema hold promise for the development of functional food.

Association of foods consumption and physical activity with prefrailty and frailty among Chinese older adults in urban communities: A cross-sectional study.

Frailty has become a public health challenge in China. To investigate the association of foods consumption and physical activity with prefrailty and frailty among older Chinese adults in urban communities. In a cross-sectional study from February to July 2023, 1183 older adults aged between 65y-88y were enrolled from urban communities in Chongqing and Shandong province, China. Frailty Index (FI) was applied to measure prefrailty and frailty. Partial proportional odds model was used to assess the association between foods consumption, physical activity and prefrailty/frailty. Higher Dietary Diversity Score (DDS), (OR=0.61, 95% CI=0.46-0.80; OR=0.47, 95% CI=0.28-0.79), Consuming animal-based foods ≥2 times/day (OR=0.62, 95% CI=0.47-0.82; OR=0.54, 95% CI=0.33-0.88), soy products ≥2 times/week (OR=0.69, 95% CI=0.53-0.89; OR=0.51, 95% CI=0.31-0.84), fresh vegetables ≥2 times/day (OR=0.42, 95% CI=0.31-0.57; OR=0.41, 95% CI=0.23-0.72), and nuts ≥2 times/week (OR=0.71, 95% CI=0.55-0.91; OR=0.52, 95% CI=0.32-0.85) was associated with a lower risk of prefrailty and frailty. In addition, higher frequency and longer duration of walking (OR=0.61, 95% CI=0.42-0.88; OR=0.63, 95% CI=0.48-0.81), exercise (OR=0.48, 95% CI=0.35-0.64; OR=0.44, 95% CI=0.32-0.61) per week were significantly associated with lower risk of prefrailty. Furthermore, higher frequency and longer duration of walking (OR=0.42, 95% CI=0.25-0.72; OR=0.46, 95% CI=0.29-0.74), and housework (OR=0.39, 95% CI=0.24-0.65; OR=0.57, 95% CI=0.34-0.96) per week, were significantly associated with lower frailty. Higher DDS and higher frequency of animal-based foods, soy products, fresh vegetables, and nuts consumption is significantly associated with lower risk of prefrailty and frailty. Additionally, walking and exercising are significantly associated with lower risk of prefrailty, while walking and doing housework is significantly associated with lower frailty.

Genomic and metabolomic analysis of Latilactobacillus sakei DCF0720 for black soybean yogurt fermentation

Genomic and metabolomic analysis of Latilactobacillus sakei DCF0720 for black soybean yogurt fermentation

Commercial Soyabean Farming and Household Livelihoods in Kole District northern Uganda, a critical analysis of Farmers Cooperative Society

This paper presents the relationship between commercial soyabean farming and household livelihoods of members of Alito Joint Christian Farmers’ Cooperative Society Limited (AJCFSL) in Kole district, Uganda. We used mixed method approach and generated quantitative and qualitative data from registered members of AJCFSL. We sampled 155 respondents out of 259 using simple random technique and purposive sampling techniques and collected data using questionnaires and interview guide. The findings reveal that the number of acres cultivated by soyabean farmers (p<0.01, β= 0.637) is significant in predicting household food security, income, provision of basic needs and responding to emergencies. However, owning storage facilities by farming households is least significant in explaining the contribution of soyabean farming to household livelihood elements (p<0.01, β= 0.216). The study concludes that planting soya bean on more acres strongly enhance family stability. The study recommends the introduction of easy means of acquiring land titles by farmers; and the introduction of subsidized tractor-hire services to boost production of soya bean and achieve a stable family environment. Further research can be considered with a view of analyzing the relationship between commercial soya bean farming and the financial, human, and physical capitals of farmers in Kole district

Impact of crop residue removal on crop production, feedstock quality, and theoretical ethanol production in the Mid‐Atlantic United States

AbstractCellulosic biomass‐to‐bioenergy systems provide fuel, reduce emissions, and offer economic benefits. Corn (Zea mays L.) and wheat (Triticum aestivum L.) residues could be used as feedstocks for biofuel production. However, the impact of residue removal on crop productivity in the Mid‐Atlantic region has not been thoroughly assessed. A trial was conducted to assess crop yield and quality response to different biomass retention rates in grain cropping systems during 2015–2017. Various combinations of corn stover (0–10 Mg ha−1) and wheat straw (0–3 Mg ha−1) were applied in a corn–wheat/soybean [Glycine max (L.) Merr.] rotation in New Kent, VA. In Blacksburg, VA, corn stover (0–20 Mg ha−1) was applied in the continuous corn system. Residues were applied after grain harvest over two production cycles for each system. Residue retention showed no significant impact on grain or crop residue yields or nutrient uptake in either system. Treatment minimally impacted feedstock quality, except wheat straw's sulfur (S) concentration, optimized at around 70% retention in New Kent. Theoretical ethanol potential (TEP) and yield remained unaffected by total residue rates in New Kent. In Blacksburg, over 2 years, a minimum TEP for corn stover corresponded to a retention rate of approximately 30%. A retention rate of more than 30% increased TEP, likely due to improved feedstock quality. Nutrient replacement costs for primary macronutrients and S uptake ranged from $18.3 to $36.9 ha−1 for corn stover and $6.1 to $11.8 ha−1 for wheat straw. Residue harvest or addition did not harm short‐term biomass yield in Virginia's grain‐based cropping systems.

Soybean response to high‐speed planting in Mississippi

AbstractMississippi soybean [Glycine max (L.) Mer.] producers are under pressure to plant as much land as possible within narrow planting windows. The 5‐year average planting progress is 45% of the total at the end of the optimal soybean planting window. New metering and seed delivery technology claims faster planting without sacrificing singulation, stand, or yield, but these tools need to be validated before recommendation. This study aimed to quantify soybean response to planting speeds using a precision planter (John Deere MaxEmerge 2 row units retrofitted with Ag Leader SureSpeed and SureForce) and a mechanical planter (John Deere 1700 ground‐driven mechanical planter equipped with eSet meters) for a total of 7 site‐years across Mississippi. In 2022, both planters were evaluated at four actual ground speeds of 7.9, 10.8, 13.5, and 16.4 km h−1 in a 2 × 4 factorial design. The experimental design was modified in 2023, where the mechanical planter served as the current farmer practice check at 9.7 km h−1and the precision planter speeds were 9.7, 14.5, and 17.7 km h−1 at research station sites and 9.7 and 14.5 km h−1 at an on‐farm site. Across sites, increased planting speed generally increased plant spacing, in‐row spacing variability, and decreased plant population. However, increased speed did not affect soybean yield. The precision planter at 17.7 km h−1 was no different from the mechanical planter in terms of soybean plant population, spacing, and yield in 2023. Results suggest soybean producers can plant soybean at 17.7 km h−1 without compromising yield.

Non-CG DNA hypomethylation promotes photosynthesis and nitrogen fixation in soybean

Non-CG DNA methylation, a plant-specific epigenetic mark mainly regulated by chromomethylase (CMT), is known to play important roles in Arabidopsis thaliana. However, whether and to what extent non-CG DNA methylation modulates agronomic traits in crops remain to be explored. Here, we describe the consequences of non-CG DNA hypomethylation on development, seed composition, and yield in soybean (Glycine max). We created a Gmcmt mutant line lacking function of all four CMT genes. This line exhibited substantial hypomethylation of non-CG (CHG and CHH) sites. Non-CG hypomethylation enhanced chromatin accessibility and promoted or repressed the expression of hundreds of functionally relevant genes, including upregulation of GOLDEN-LIKE 10 (GmGLK10), which led to enhanced photosynthesis and, unexpectedly, improved nitrogen fixation efficiency. The Gmcmt line produced larger seeds with increased protein content. This study provides insights into the mechanisms of non-CG methylation-based epigenetic regulation of soybean development and suggests viable epigenetic strategies for improving soybean yield and nutritional value.

Detection of yellow mosaic virus resistance in Soybean (Glycine max L.) genotypes for yield and related traits

Detection of yellow mosaic virus resistance in Soybean (Glycine max L.) genotypes for yield and related traits

Application of biohybrid membranes for arsenic and chromium removal and their impact on pollutant accumulation in soybean (Glycine max L.) seedlings.

Chromium and arsenic are among the priority pollutants to be controlled by regulatory and health agencies due to their ability to accumulate in food chains and the harmful effects on health resulting from the ingestion of food contaminated with metals and metalloids. In the present work, four biohybrid membrane systems were developed as alternatives for the removal of these pollutants, three based on polyvinyl alcohol polymeric mesh (PVA, PVA-magnetite, PVA L-cysteine) and one based on polybutylene adipate terephthalate (PBAT), all associated with bioremediation agents. The efficiency of the bioassociation process was assessed through count methods and microscopy. The removal capacity of these systems was evaluated in synthetic liquid medium, both in the absence and in the presence of soybean (Glycine max L.) seedlings. The content of chromium and arsenic was also analyzed in aerial and hypogeous tissues of seedlings grown on contaminated solid substrate. PVA and PVA-magnetite biohybrid membranes showed the highest removal rates, between 57 and 75% of the initial arsenic content and more than 80% of the initial chromium content after 48h of treatment, when evaluated in synthetic liquid media with initial concentrations of 2.5ppm of pentavalent arsenic and 5ppm of hexavalent chromium, both in presence and absence of seedlings. PVA and PBAT promoted a significant reduction of arsenic translocation to the aerial parts, generally edible, of this crop of agronomic interest. The systems tested showed a high potential for biotechnological applications in matrices affected by the presence of arsenic and chromium.

PENGARUH PEMBERIAN SUSU KEDELAI TERHADAP PENINGKATAN PRODUKSI ASI PADA IBU POSTPARTUM

Maternal deaths occur after childbirth in around 60% of cases, and postpartum obstetric care with monitoring eliminates 50% of these deaths. The puerperium is a critical time for mothers. Breast milk is essential for preserving an infant's health and survival. Breastfeeding should begin as soon as possible, ideally in the first 30 to 60 minutes following delivery.The goal of the baby's suction on the mother's nipple is not to extract a large amount of milk. Soy milk or soy beans are commonly available and reasonably priced, and they can facilitate a smoother nursing experience. This study aimed to ascertain whether providing soy milk to postpartum moms could enhance their supply of breast milk. In this work, a case study methodology was combined with descriptive research techniques. Mrs. "D," who is receiving care at Hj. Bunda Halimah Hospital in Batam City, has an enhanced milk production that is measured by using observation sheet with LATCH scores. The results of the study after being given soy milk 2 times in 3 consecutive days with a LATCH score of 9 (good). The conclusion of midwifery care in postpartum mothers by giving soy milk times in 3 consecutive days has an effect on increasing Mrs. D's milk production

Effect of Fermented Soy Beverage on Equol Production by Fecal Microbiota

Soy consumption is associated with health benefits, mainly linked to the ability of the intestinal microbiota to metabolize the glycosylated isoflavones into more bioactive compounds, such as equol. Because Bifidobacterium pseudocatenulatum INIA P815 is able to efficiently deglycosylate daidzin into daidzein, the aim of this work was to confirm the influence of soy beverages fermented by B. pseudocatenulatum INIA P815 for enhancing equol production by fecal microbiota. Firstly, fecal samples from 17 participants were characterized in vitro, and we observed that 35.3% of them were able to produce equol from daidzein. In addition, the kinetics of equol production and degradation by fecal microbiota were evaluated, determining that 30–85% of equol is degraded after 24 h of incubation. Finally, the influence of fermented soy beverage on improving the production of equol by selected equol-producing fecal samples and by the equol-producing strain Slackia isoflavoniconvertens was analyzed through a colonic model. Fermented soy beverage enhanced the equol production from S. isoflavoniconvertens as well as the fecal samples whose microbiota showed high rates of equol degradation. The results obtained confirm that the fermentation of soy beverages with selected bacterial strains improves the functional properties of these beverages in terms of isoflavone metabolism and equol production.

Characterization of Key Aroma Compounds of Soy Sauce-like Aroma Produced in Ferment of Soybeans by Bacillus subtilis BJ3-2

Fermented soybeans are popular among many for their rich soy sauce-like aroma. However, the precise composition of this aroma remains elusive, with key aroma compounds unidentified. In this study, we screened the candidate genes ilvA and serA in BJ3-2 based on previous multi-omics data, and we constructed three mutant strains, BJ3-2-ΔserA, BJ3-2-ΔilvA, and BJ3-2-ΔserAΔilvA, using homologous recombination to fermented soybeans with varying intensities of soy sauce-like aroma. Our objective was to analyze samples that exhibited different aroma intensities resulting from the fermented soybeans of BJ3-2 and its mutant strains, thereby exploring the key flavor compounds influencing soy sauce-like aroma as well analyzing the effects of ilvA and serA on soy sauce-like aroma. We employed quantitative descriptive sensory analysis (QDA), gas chromatography–olfactometry–mass spectrometry (GC-O-MS), relative odor activity value analysis (rOAV), principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and partial least squares regression analysis (PLSR). QDA revealed the predominant soy sauce-like aroma profile of roasted and smoky aromas. GC-MS detected 99 volatile components, predominantly pyrazines and ketones, across the four samples, each showing varying concentrations. Based on rOAV (>1) and GC-O, 12 compounds emerged as primary contributors to soy sauce-like aroma. PCA and OPLS-DA were instrumental in discerning aroma differences among the samples, identifying five compounds with VIP > 1 as key marker compounds influencing soy sauce-like aroma intensity levels. Differential analyses of key aroma compounds indicated that the mutant strains of ilvA and serA affected soy sauce-like aroma mainly by affecting pyrazines. PLSR analysis indicated that roasted and smoky aromas were the two most important sensory attributes of soy sauce-like aroma, with pyrazines associated with roasted aroma and guaiacol associated with smoky aroma. In addition, substances positively correlated with the intensity of soy sauce-like aroma were verified by additional experiments. This study enhances our understanding of the characteristic flavor compounds in soy sauce-like aroma ferments, provides new perspectives for analyzing the molecular mechanisms of soy sauce-like aroma formation, and provides a theoretical framework for the targeted enhancement of soy sauce-like aroma in various foods.

In vitro adventitious root culture system for optimal production of genistein in soybean (Glycine max L. Merrill)

In vitro adventitious root culture system for optimal production of genistein in soybean (Glycine max L. Merrill)

Integration of bare soil and crop growth remote sensing data to improve the accuracy of soil organic matter mapping in black soil areas

Accurately mapping the spatial distribution of soil organic matter (SOM) content is critical for informed land management decisions and comprehensive climate change analyses. Remote sensing-based SOM mapping models during periods of bare soil exposure have demonstrated efficacy in various regional studies. However, integrating bare soil imagery with growing season imagery for SOM content mapping remains a complex process. We conducted a study in Youyi Farm, a representative area of black soil in Northeast China. We collected 574 soil samples (0–20 cm) with SOM content through field sampling and laboratory analysis. Additionally, cloud-free Sentinel-2 images were obtained from the Google Earth Engine (GEE) platform for both the bare soil period (April-June, October) and crop growth period (July-September) from 2019 to 2021. To assess the influence of crop growth information on SOM mapping, we incorporated remote sensing imagery during the crop growth period, considering different crop type zones (maize (Zea mays L.), soybean (Glycine max L.), and rice (Oryza sativa L.)). We conducted overall and zonal regressions using the random forest (RF) model to validate the prediction results through cross-validation. Our findings indicate that: (1) adding crop growth period images to the bare soil period images in different years can improve the accuracy of SOM mapping. For example, in the overall regression model of 2020, the highest accuracy was achieved by using the combination of May-July images, with an R2 value of 0.70 and an RMSE value of 0.71 %; (2) zonal regression by differentiating crop types can effectively improve the SOM mapping accuracy. In 2019, using zonal regression, the R2 of SOM mapping accuracy was improved by 0.02 and the RMSE was reduced by 0.03 % compared with the overall regression; (3) precipitation is an important factor affecting the accuracy of SOM prediction, and the lower the precipitation, the higher the accuracy of SOM prediction. In summary, the results of this study show that in the SOM remote sensing mapping of the black soil area, the growing period remote sensing information of different crop types should be comprehensively considered and combined with the image data of the years of lower precipitation, the accuracy of the SOM mapping can be effectively improved, which provides a new technological path and an application basis for the enhancement of the accuracy in remote sensing mapping with soil attributes.

Proximate Composition and Consumer Acceptability of Powdered Fermented Locust Bean and Soybean Condiments

Proximate Composition and Consumer Acceptability of Powdered Fermented Locust Bean and Soybean Condiments

Effect of Fermented Soybean (FSB) Supplementation on Gastroesophageal Reflux Disease (GERD).

Gastroesophageal reflux disease (GERD) is a prevalent chronic condition affecting the well-being of both adults and children in general medical practice. Research on the effects of fermented soybean (SB) supplementation in managing GERD is relatively new, with limited studies available. The existing research often lacks sufficient dosing regimens and study durations to differentiate between transient placebo effects and sustained benefits. In this study, the beneficial effects of FSB supplementation were investigated in 110 voluntary participants (NCT06524271). The participants were required to take 1 g of FSB supplement once daily for 12 weeks. GERD symptoms were evaluated using the Reflux Disease Questionnaire (RDQ), while inflammatory markers, including interleukin-4 (IL-4), interleukin-6 (IL-6), and interleukin-8 (IL-8), were measured to assess inflammation. The Quality of Life in Reflux and Dyspepsia (QOLRAD) questionnaire was used to evaluate participants' quality of life. The results indicated that FSB supplementation significantly (p < 0.05) alleviated heartburn and regurgitation symptoms and reduced levels of IL-4, IL-6, and IL-8, indicating a notable anti-inflammatory effect. Additionally, significant (p < 0.05) improvements were observed in QOLRAD scores, particularly in vitality, emotional distress, and physical/social functioning. Collectively, our findings support the use of FSB as an adjuvant approach in managing GERD, with notable improvements in patients' quality of life.

Morpho-phenological responses of early and late soybean maturity groups to diverse day lengths and temperature regimes

On a global scale, soybean (Glycine max (L.) Merr.) is cultivated across a wide range of environmental conditions, characterized by variable temperatures and day lengths. It is important to gather information about different maturity group’s phenology and growth patterns to enhance agricultural practices and develop various soybean crop models. We conducted two experiments focusing on soybean maturity groups, commonly used cultivars for early and late maturity groups (one cultivar for each maturity group), one with varying day/night temperatures (22/14, 26/18, 30/22, 34/26, and 40/32°C) under a constant day length of 12.5/11.5 hours. Second, we investigated day lengths of 12.0, 12.5, 13.0, 13.5, 14.0, and 14.5 hours under constant temperature (26°C). We examined various morpho-phenological traits, including plant height, branch number per plant, vegetative development rate, and days to the first flower. Long-day lengths were found to increase the number of branches per plant, whereas shorter-day lengths led to earlier flowering in the cultivars utilized for the experiment. The duration until the first flower was prolonged under lower temperatures and longer day lengths. As a result, understanding the impacts of temperatures and day lengths and their interactions during the leaf, branch, and flowering stages will significantly contribute to improving seed production in future environmental conditions. Furthermore, the insights gained from this study will play an important role in enhancing soybean breeding programs and improving soybean crop models.

Effect of insecticides and intercropping systems on fall armyworm (Spodoptera frugiperda (J.E. Smith)) infestations and damage in maize in northern Ghana

Field studies were conducted over two successive seasons to test the effects of maize-legume intercropping system on fall armyworm (FAW) (Spodoptera frugiperda (J.E. Smith)) and if insecticide use was necessary in intercropping systems. Three insecticide treatments [no spray, Emastar 112 EC (a.i. emamectin benzoate 48 g/L + acetamiprid 64 g/L), Neem seed oil (NSO) (a.i. 3% azadirachtin)] and three intercropping systems [sole maize (Zea mays L.), maize + soybean (Glycine max (L.) Merr.) and maize + groundnut (Arachis hypogaea L.)] were arranged as a 3 × 3 factorial experiment in a randomized complete block design. Data were collected on FAW larval infestations, parasitism rates, damage, grain yield and gross financial returns. Results from combined-years analyses showed that larval infestation was significantly affected by intercropping (p = 0.034) only, with maize + soybean recording the lowest infestation while sole maize was highest. Damage to crop and cobs were significantly affected by years and insecticide treatments. Cob damage was also significantly affected by intercropping system with maize + soybean being lowest and sole maize recording the highest damage. Parasitoids recorded were Coccygidium luteum, Chelonus bifoveolatus, and Charops sp. Grain yield was significantly affected by the intercropping systems only (p = 0.004), with no significant year (p = 0.152), insecticide (p = 0.726) and insecticide × intercropping system (p = 0.660) effects. Sole maize (1.6 t/ha) had the lowest yield while maize + groundnut (2.2 t/ha) was highest. In terms of income, an economic analysis showed that spraying intercropped maize with NSO resulted in the highest marginal rate of return. Thus, the use of NSO and intercropping is an effective FAW management strategy.

Genome-Wide Characterization of Glyceraldehyde-3-Phosphate Dehydrogenase Genes and Their Expression Profile under Drought Stress in Quercus rubra.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is crucial in plant metabolism and responses to various abiotic stresses. In the glycolysis pathway, glyceraldehyde-3-phosphate (G3P) is oxidized to 1,3-bisphosphate glycerate (1,3-BPG) through the catalytic action of GAPDH. However, the GAPDH gene family in Quercus rubra has been minimally researched. In this study, we identified 13 GAPDH-encoding genes in Q. rubra through a bioinformatics analysis of genomic data. Evolutionary studies suggest that these QrGAPDH genes are closely related to those in Glycine max and Triticum aestivum. We conducted a comprehensive whole-genome study, which included predictions of subcellular localization, gene structure analysis, protein motif identification, chromosomal placement, and analysis of cis-acting regions. We also examined the expression of GAPDH proteins and genes in various tissues of Q. rubra and under drought stress. The results indicated diverse expression patterns across different tissues and differential expression under drought conditions. Notably, the expression of Qurub.02G290300.1, Qurub.10G209800.1, and Qrub.M241600.1 significantly increased in the leaf, stem, and root tissues under drought stress. This study provides a systematic analysis of QrGAPDH genes, suggesting their pivotal roles in the drought stress response of trees.

Improving natto quality through Co-fermentation of functionally complementary Bacillus and Lactiplantibacillus species

Natto, a nutritious fermented soybean product, is known for its health benefits. Modern production methods use Bacillus subtilis as the starter culture to ensure consistent quality. However, this method overlooks the potential positive effects of other microbial species present in traditionally, naturally fermented natto. This study explored the effects of ARTP-induced mutants of B. subtilis var. natto and several strains of lactic acid bacteria (LAB) on natto quality. The results showed that these two types of bacteria have complementary roles in fermentation. B. subtilis var. natto, with enhanced extracellular protease activity, promotes the hydrolysis of soy protein and enhances the product's ACE inhibitory and fibrinolytic activities. Meanwhile, two strains of Lactiplantibacillus plantarum reduced the content of total volatile basic nitrogen (TVB-N) while increasing the content of folic acid in the final product. In addition, B. subtilis var. natto and LAB produce different profiles of volatile compounds (VOCs). Subsequently, B. subtilis var. natto and Lpb. plantarum were used to co-ferment natto in different ratios. The results showed that the two types of bacteria could co-colonize and enhance fermentation quality through their respective strengths. Specifically, Lpb. plantarum strains co-metabolized with B. subtilis var. natto to elevate the content of pyrazines, characteristic VOCs in natto. The optimal quality of natto was achieved when Lpb. plantarum HIS6, Lpb. plantarum LP3 and B. subtilis var. natto Y15-11 were combined in a 2:1:2 ratio. This study not only identified effective combinations of starter cultures with complementary benefits but also elucidated the metabolic pathways of key volatile compounds. Our findings also provide technical insights for improving natto quality, enhancing consumer acceptance, and supporting industrial production.