Regular physical activity (PA) and low levels of sedentary behavior (SB) have positive health effects on young people. Adolescent girls of low socioeconomic background represent a high-risk group with regard to physical inactivity and SB. In this study, accelerometer-measured levels of PA and SB of female sixth graders attending lower secondary schools in Germany are presented, patterns of PA and SB throughout the day are described and differences between weekdays and weekend days are analyzed. Data of 425 students of the CReActivity project were analyzed. Sampling and processing of accelerometer data followed recent recommendations, which had not been applied to data of a German-speaking sample before. The WHO recommendation of daily 60 min moderate-to-vigorous PA was fulfilled by 90.4% of the girls on weekdays and by 57.4% on weekend days. The significant weekday–weekend differences were mainly associated with active commuting to and from school. Students engaged in SB for more than 8 h on weekdays and for over 7 h on weekend days. The results suggest a strong need for interventions increasing PA and reducing SB, especially during school hours and on weekends. Furthermore, a comparison with methods and results of previous studies highlights the need to follow recent criteria in accelerometer data sampling and processing to ensure an accurate and valid differentiation between PA-related risk groups and non-risk groups.