Feedstock For Production Research Articles

Development and utilization of genome-wide InDel markers in Sorghum [Sorghum bicolor (L.) Moench]

Sorghum (Sorghum bicolor L. Moench) has become an increasingly valuable crop for food, feed, and especially bioenergy feedstock production, which makes the crop extremely attractive for studying genomics and genetic diversity. Molecular markers and genomics play essential roles in sorghum breeding. The rapid development of next-generation sequencing technology has facilitated the identification of genome-wide insertion-deletion (InDel) polymorphisms, enabling the efficient construction of InDel markers that are suitable for user-friendly PCR. This study was conducted with the objective of discovering and developing InDel markers using double digest restriction site-associated DNA sequencing (ddRAD-Seq) data. A total of 19,226 InDels distributed across 10 chromosomes in the sorghum genome was identified. Of those, deletions constituted 65.7% while the remain was insertions. A comprehensive analysis of all the chromosomes revealed a total of 80 InDel sites with a minimum length of 10 bp. For a good conversion of the InDel regions to beneficial molecular markers, specific primers were designed for the amplification of 47 InDel regions that were selected for further investigation. A diverse panel of sorghum consisting of 16 accessions served a source for the developed InDel markers validation. Of the 47 InDel markers, 14 were tested across 16 sorghum accessions and were demonstrated their helpfulness for marker-assisted selection in sorghum. The polymorphic information content (PIC) values of the 16 markers varied between 0.11 and 0.38, with an average of 0.28. The findings of this study indicated that the identification of InDels and the development of molecular markers for sorghum were accomplished using the ddRAD-Seq data.

Biological Upcycling of Plastics Waste.

Plastic wastes accumulate in the environment, impacting wildlife and human health and representing a significant pool of inexpensive waste carbon that could form feedstock for the sustainable production of commodity chemicals, monomers, and specialty chemicals. Current mechanical recycling technologies are not economically attractive due to the lower-quality plastics that are produced in each iteration. Thus, the development of a plastics economy requires a solution that can deconstruct plastics and generate value from the deconstruction products. Biological systems can provide such value by allowing for the processing of mixed plastics waste streams via enzymatic specificity and using engineered metabolic pathways to produce upcycling targets. We focus on the use of biological systems for waste plastics deconstruction and upcycling. We highlight documented and predicted mechanisms through which plastics are biologically deconstructed and assimilated and provide examples of upcycled products from biological systems. Additionally, we detail current challenges in the field, including the discovery and development of microorganisms and enzymes for deconstructing non-polyethylene terephthalate plastics, the selection of appropriate target molecules to incentivize development of a plastic bioeconomy, and the selection of microbial chassis for the valorization of deconstruction products.

Polyhydroxyalkanoate Production from Eucalyptus Bark's Enzymatic Hydrolysate.

In recent years, polyhydroxyalkanoates (PHAs) have gained notoriety because of their desirable properties that include proven biodegradability, biocompatibility, and thermal stability, which make them suitable alternatives to fossil-based polymers. However, the widespread use of PHAs is still challenging because of their production costs, which are greatly associated with the cultivation medium used for bacterial cultivation. In Portugal, one-quarter of the forest area is covered by Eucalyptus globulus wood, making its residues a cheap, abundant, and sustainable potential carbon source for biotechnological uses. In this work, eucalyptus bark was used as the sole feedstock for PHA production in a circular bioeconomic approach. Eucalyptus bark hydrolysate was obtained after enzymatic saccharification using Cellic® CTec3, resulting in a sugar-rich solution containing glucose and xylose. Although with differing performances, several bacteria were able to grow and produce PHA with distinct compositions, using the enzymatic hydrolysate as the sole carbon source. Pseudomonas citronellolis NRRL B-2504 achieved a high cellular growth rate in bioreactor assays (24.4 ± 0.15 g/L) but presented a low accumulation of a medium-chain-length PHA (mcl-PHA) comprising the monomers hydroxydecanoate (HD, 65%), hydroxydodecanoate (HDd, 25%), and hydroxytetradecanoate (HTd, 14%). Burkholderia thailandensis E264, on the other hand, reached a lower cellular growth rate (8.87 ± 0.34 g/L) but showed a higher biopolymer accumulation, with a polyhydroxybutyrate (PHB) content in the cells of 12.3 wt.%. The new isolate, Pseudomonas sp., revealed that under nitrogen availability, it was able to reach a higher accumulation of the homopolymer PHB (31 wt.%). These results, although preliminary, demonstrate the suitability of eucalyptus bark's enzymatic hydrolysate as a feedstock for PHA production, thus offering an exciting avenue for achieving sustainable and environmentally responsible plastic products from an undervalued forestry waste.

Anaerobic co-digestion of microalgal biomass, sugarcane vinasse, and residual glycerol from biodiesel using simplex-centroid mixture design: methane potential, synergic effect, and microbial diversity.

Microalgal biomass (MB) is a promising feedstock for bioenergy production. Nonetheless, the cell recalcitrance and the low C/N ratio limit the methane yield during anaerobic digestion. As an alternative to overcome these challenges, MB co-digestion with different feedstocks has been proposed. Thus, this study evaluated the anaerobic co-digestion (AcoD) of MB cultivated in wastewater with sugarcane vinasse (VIN) and residual glycerol from biodiesel production (GLY). Batch tests were conducted using augmented simplex-centroid mixture design to investigate the impact of AcoD on methane production (SMP), synergistic effects, and the influence on microbial community. When compared to MB digestion, 150 NmL CH4.g-1VS, binary and ternary AcoD achieved SMP increases from 120 to 337%. The combination of 16.7:16.7:66.7 (MB:VIN:GLY) showed the highest SMP for a ternary mixture (631 NmL CH4.g-1VS). Optimal synergies ranged from 1.3 to 1.4 and were primarily found for the MB:GLY AcoD. Acetoclastic Methanosaeta genus was predominant, regardless the combination between substrates. Despite the largest SMP obtained from the MB:GLY AcoD, other ternary mixtures were also highly synergetic and therefore had strong potential as a strategic renewable energy source.

A sustainable synthesis of polyhydroxyalkanoate from stubble waste as a carbon source using Pseudomonas putida MTCC 2475.

Polyhydroxyalkanoates (PHAs) are biodegradable polymers that can be produced from lignocellulosic biomass by microorganisms. Cheap and readily available raw material, such as corn stover waste, has the potential to lessen the cost of PHA synthesis. In this research study, corn stover is pretreated with NaOH under conditions optimized for high cellulose and low lignin with central composite design (CCD) followed by characterization using Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and scanning electron microscopy (SEM). Design expert software performed further optimization of alkali pretreated corn stover for high total reducing sugar (TRS) enhancement using CCD using response surface methodology (RSM). The optimized condition by RSM produced a TRS yield of 707.19mg/g. Fermentation using corn stover hydrolysate by Pseudomonas putida MTCC 2475 gave mcl-PHA detected through gas c hromatography - t andem m ass s pectrometry (GC-MS/MS) and characterization of the PHA film by differential scanning calorimetry (DSC), FTIR, and nuclear magnetic resonance (NMR). Thus, this research paper focuses on using agriculture (stubble) waste as an alternative feedstock for PHA production.

Animal Manure as an Alternative Bioenergy Resource in Rural Sub-Saharan Africa: Present Insights, Challenges, and Prospects for Future Advancements

Energy availability is a pivotal driver in fostering sustainable socio-economic development. However, sub-Saharan Africa (SSA) grapples with paradoxes headlined by abundant energy resources but with the world’s lowest access to clean energy index per capita. Faced with a lack of access to clean energy sources like electricity, rural areas in the majority of SSA countries almost exclusively depend on biomass-fuels, mostly fuelwood, leading to heightened respiratory health risks as well as environmental degradation and accelerated climate change. As an alternative, this review investigates the potential of animal manure as a sustainable energy resource for rural SSA households, emphasising its utilisation as a feedstock for biogas production using anaerobic digestor technology. Results show that despite the abundance of literature that reports on successes in lab-scale bioreactor optimisation, as well as successes in the initial rollout of biogas biodigester technology in SSA with the help of international collaborators, the actual uptake of biogas bioreactor technology by rural communities remains low, while installed bioreactors are experiencing high failure rates. Resultantly, rural SSA still lags significantly behind in the adoption of sustainable clean energy systems in comparison to rural communities in other regions. Among some of the hurdles identified as driving low technology assimilation are onerous policy requirements, low-level government involvement, high bioreactor-instalment costs, the lack of training and awareness, and water scarcity. Prospects for success lie in innovative technologies like the low-cost portable FlexiBiogas system and private–public partnerships, as well as flexible energy policy frameworks. Bridging the knowledge-implementation gap requires a holistic approach considering cultural, technological, and policy aspects.

Nutrients recovery from dairy wastewater by Chlorella vulgaris and comparison of the lipid's composition with various chlorella strains for biodiesel production.

Microalgae biomass is regarded as a promising feedstock for biodiesel production. The biomass lipid content and fatty acids composition are among the main selective criteria when screening microalgae strains for biodiesel production. In this study, three strains of Chlorella microalgae (C. kessleri, C. sorokiniana, C. vulgaris) were cultivated nutrient media with different nitrogen contents, and on a medium with the addition of dairy wastewater. Moreover, microalgae grown on dairy wastewater allowed the removal of azote and phosphorous. The removal efficiency of 90%, 53% and 95% of ammonium nitrogen, total nitrogen and phosphate ions, respectively, were reached. The efficiency of wastewater treatment from inorganic carbon was 55%, while the maximum growth of biomass was achieved. All four samples of microalgae had a similar fatty acid profile. Palmitic acid (C16:0) was the most abundant saturated fatty acid (SFA), and is suitable for the production of biodiesel. The main unsaturated fatty acids (UFA) present in the samples were oleic acid (C18:1 n9); linoleic acid (C18:2 n6) and alpha-linolenic acid (C18:3 n3), which belong to omega-9, omega-6, omega-3, respectively.

Enhanced Scenedesmus obliquus Cultivation in Plastic-Type Flat Panel Photobioreactor for Biodiesel Production

A plastic-type flat panel photobioreactor (PTFPP) prototype was designed for microalgae cultivation as biodiesel feedstock. The growth, biomass, and lipid production of the oleaginous microalga Scenedesmus obliquus were optimized through the enhanced design and cultivation conditions in the PTFPP. The optimization conditions include cultivation of the microalga in a flat panel photobioreactor manufactured from a 10 µm-thick plastic sheet with dimensions of 40 cm in width and 60 cm in height. The width of the designed plastic bags was adjusted by “4 ports” of circular adhesion points which make the volumetric cultural capacity 5 L. Cultivation of the microalga was optimized through the replacement of the sodium nitrate of the BBM medium with urea as a nitrogen source. Cultivation bags were subjected to continuous illumination with 3000 lux white, fluorescent lamps and aerated with 1.5 L air/min (equal to 0.3 VVM). Biomass production from the designed PTFPP reached 3 g/L with around 40% lipid content (on a dry weight basis). Based on a GC-MS analysis of the produced fatty acid methyl ester (biodiesel) from S. obliquus, the percentage of C16 and C18 fatty acids reached more than 90% of the defined fatty acids. Out of this percentage, 66.6% were unsaturated fatty acids. The produced fatty acid profile of the S. obliquus biomass cultivated in the designed PTFPP prototype could be considered a suitable feedstock for biodiesel production.

Feasibility Study of Developing an Eco-Friendly Bio Lubricant from Flaxseed Oil

The increasing global demand for sustainable and environmentally friendly lubricants has led to the exploration of bio-based alternatives. This study focuses on the production of a bio- lubricant using Flax Seed oil as the feedstock through an epoxidation process followed by acid testing. The first step involves the epoxidation of Flax Seed, where the unsaturated fatty acids present in the oil are converted into epoxides. This process is carried out using a catalyst and a peroxide source. The reaction conditions, such as temperature, time, and reactant ratios, are optimized to achieve the highest conversion and selectivity of epoxidized Flax Seed oil. The epoxidized Flax Seed is then subjected to acid testing to evaluate its suitability as a bio- lubricant. The acid value, which measures the amount of acidic compounds present, is determined. A low acid value indicates good stability and resistance to oxidation. Additionally, the viscosity, flash point and other relevant properties are assessed to ensure that the bio- lubricant meets industry standards. The results of the acid testing provide valuable insights into the quality and performance of the produced bio-lubricant. By analyzing the acid value and other properties, the effectiveness of the epoxidation process and the feasibility of using Flax Seed oil as a sustainable feedstock for bio-lubricant production can be determined. This research contributes to the development of eco-friendly lubricants by utilizing Flax Seed oil as a renewable resource. The epoxidation process and subsequent acid testing serve as crucial steps in the production and evaluation of bio-lubricants, highlighting their potential as a viable alternative to conventional petroleum-based lubricants

Advances in the Homogeneously Catalyzed Hydrogen Production from Biomass Derived Feedstocks: a Review.

Biomass‐derived feedstocks for hydrogen production are crucial as an alternative to fossil fuel especially in those areas where green electricity and clean water are scarce. In this framework the transformation of simple (formic acid, alcohols) and more complex (polyalcohols, sugars and cellulose) bio‐derivatives in pure hydrogen is recognized as a promising approach. Parallel to great effort in heterogeneous catalysis, milder molecular systems represent a more selective eye for alternative solutions and mechanistic insights. In the present review the introduction summarizes the challenges in the catalytic utilization of biomass‐derived feedstocks, followed by the advances in homogeneously catalyzed hydrogen production from different substrates which will cover formic acid, with oustanding efficiency with noble metals and promising results with earth abundant ones and alcohols and polyalcohols, with particular emphasis to the development of heterogenized systems, ligand assisted catalysts and bi‐catalytic synergistic solutions which allow to avoid base and to promote catalyst stability and recyclability. In the last part, description of hydrogen production from more complex substrates, such as sugars and cellulose, will show the role of molecular complexes in main and side reactions. Critical comments on the reported advances are provided along the whole discussion.

Copper nanoclusters: Selective CO2 to methane conversion beyond 1 A/cm²

Carbon dioxide offers a unique opportunity as a feedstock for energy production through electrocatalysis. Methane production holds promise for its widespread applications and market demand. However, commercial viability faces challenges of low selectivity, current density, and high applied potential. Efforts to improve methane selectivity while suppressing multi-carbon products, e.g., ethylene, often involve lower alkalinity electrolytes. However, it reduces current density due to increased ohmic resistance without significant gains in the reaction yield. This study utilizes quantum mechanics computations to design a nano-cluster copper catalyst that redirects the reaction pathway from ethylene towards methane, even under alkaline conditions. We achieved a Faradaic efficiency (FE) of 85 %, a current density of 1.5 A/cm2, and stability of over 10 hours solely by controlling particle size in copper catalysts. This work paves the way to overcoming current limitations in electrocatalytic methane production and holds broader implications for advancing sustainable CO2 utilization in energy systems.

Land-neutral negative emissions through biochar-based fertilization—assessing global potentials under varied management and pyrolysis conditions

Climate stabilization is crucial for restabilizing the Earth system but should not undermine biosphere integrity, a second pillar of Earth system functioning. This is of particular concern if it is to be achieved through biomass-based negative emission (NE) technologies that compete for land with food production and ecosystem protection. We assess the NE contribution of land- and calorie-neutral pyrogenic carbon capture and storage (LCN-PyCCS) facilitated by biochar-based fertilization, which sequesters carbon and reduces land demand by increasing crop yields. Applying the global biosphere model LPJmL with an enhanced representation of fast-growing species for PyCCS feedstock production, we calculated a land-neutral global NE potential of 0.20–1.10 GtCO2 year−1 assuming 74% of the biochar carbon remaining in the soil after 100 years (for + 10% yield increase; no potential for + 5%; 0.61–1.88 GtCO2 year−1 for + 15%). The potential is primarily driven by the achievable yield increase and the management intensity of the biomass producing systems. NE production is estimated to be enhanced by + 200–270% if management intensity increases from a marginal to a moderate level. Furthermore, our results show sensitivity to process-specific biochar yields and carbon contents, producing a difference of + 40–75% between conservative assumptions and an optimized setting. Despite these challenges for making world-wide assumptions on LCN-PyCCS systems in modeling, our findings point to discrepancies between the large NE volumes calculated in demand-driven and economically optimized mitigation scenarios and the potentials from analyses focusing on supply-driven approaches that meet environmental and socioeconomic preconditions as delivered by LCN-PyCCS.

Poly(3-hydroxybutyrate) production from cassava processing wastes by Paracoccus sp. through high cell density cultivation: Effects of substrates limitation and kinetic analysis

Polyhydroxybutyrate (PHB) is bioplastic that has been recognized as a viable alternative to petroleum-based ones, owing to its biodegradability and biocompatibility. However, its use has long been hampered by its high selling price, caused mainly by using costly fermentation feedstock. In the present study, cassava pulp (CP) was investigated as an alternative low-cost carbon source for a high cell density cultivation of Paracoccus sp. KKU01 for PHB production. CP was hydrolyzed enzymatically, and the resulting hydrolysate was used as the base medium to optimize the bacterial growth conditions, i.e., medium compositions and dissolved oxygen setpoints, attaining as high as 40.9 ± 0.0 g/L of biomass. The optimum growth conditions were subsequently used in 4 fed-batch fermentation regimes, and Regime 2, where complete medium (CP hydrolysate supplemented with other nutrients) was fed twice followed by twice feedings of concentrated CP hydrolysate (CPH), was found to give highest biomass and PHB production of 114.5 ± 1.3 and 47.8 ± 3.9 g/L, respectively, with 41.8 ± 2.9% PHB content and yield of 0.12 kg/kg-CP. PHB productivity was 0.80 ± 0.06 g/(L·h). Logistic and Luedeking-Piret models were used successfully to describe the profiles of bacterial growth and PHB production, respectively. Curve fitting using the Luedeking-Piret model revealed that PHB synthesis and PHB turnover occurred simultaneously during the process. Overall, the results demonstrated that cassava processing wastes are feasible feedstock for PHB production by Paracoccus sp. KKU01.

Intensifying outdoor cultivation of oleaginous microalgae in photobioreactors for simultaneous wastewater bioremediation and low-cost production of renewable biodiesel feedstocks

Oleaginous microalgae are gaining increasing attention for simultaneous wastewater bioremediation and biofuel feedstock production. In this study, four oleaginous species of microalgae including Chlamydomonas sp. WWP, Scenedesmus sp. HP56, Haematococcus sp., and Nannochloropsis sp., were screened for their growth and lipid production at 30, 35, and 40 °C, under light intensity at 3, 4, and 5 klux. Among the strains screened, Haematococcus sp. grew best and gave comparable high growth at 30 °C and 35 °C (0.7–0.8 g/L) indicating its thermotolerant characteristic, and the optimal light intensity was found at 4 klux. Haematococcus sp. also grew well using unsterile secondary effluent from the seafood processing plant and produced high biomass (0.53–0.57 g/L) and lipids (0.17–0.19 g/L), especially in the column photobioreactor (CP). The repeated-batch outdoor cultivation mode showed that CP equipped with four-blade baffles was most efficient for the production of biomass (0.40–0.63 g/L) and lipids (0.15–0.30 g/L) during 4 cycles. The microalga also removed total nitrogen and total phosphorus from secondary effluent by 70.59% and 94.97%, respectively. The fatty acid compositions in microalgal lipids were C16-C18, especially palmitic acid, oleic acid, and linolenic acid, which show superior fuel properties, suggesting their promising use as biodiesel feedstocks. These strategies may have a significant impact not only on bioremediation of industrial effluent but also low-cost production of microalgae-based biodiesel feedstocks.

Characterization of GPAT gene family in Euphorbia lathyris L. and elucidating the role of ElGPAT9 in the biosynthesis of oils and pollen viability

Caper spurge (Euphorbia lathyris L.), known for its high content of oleic acid-enriched seed oils, has gained recognition as a valuable non-edible feedstock for biodiesel production. However, there is limited understanding of the biosynthesis and regulation of these desirable seed oils. In this study, an omics-based approach and genetic transformation were employed to investigate the glycerol-3-phosphate acyltransferase (GPAT) enzyme, which catalyzes the initial acylation reaction in triacylglycerol (TAG) biosynthesis, in caper spurge. Ten genes encoding ElGPAT, identified from the E. lathyris genome, were classified into three phylogenetic groups, exhibiting similar protein motifs and gene structures. RNA-seq data revealed distinct expression patterns of these ElGPAT genes in various tissues and different stages of seed development. Notably, ElGPAT9 showed the highest expression in flowers and developing seeds, with its encoded protein localized in the endoplasmic reticulum. Experiments using a yeast (Saccharomyces cerevisiae) expression system demonstrated that ElGPAT9 exhibited strong GPAT enzyme activity, crucial for TAG assembly, and preference for oleic acid (C18:1), as confirmed by feeding tests with exogenous fatty acids (FA). Similarly, stable transgenic lines of Nicotiana tabacum over-expressing ElGPAT9 showed increased levels of total oil and oleic acid, as well as improved pollen viability, without any growth penalty compared to wild-type and empty-vector controls. The transcriptome based WGCNA also identified genes related to lipid synthesis that are co-expressed with ElGPAT9. These findings provide a foundation for further understanding the roles of ElGPAT family members in E. lathyris and suggest the potential of ElGPAT9 as a desirable target for genetic engineering to enhance the production of vegetable oils enriched with C18:1 in caper spurge and other oil crops, contributing to sustainable biodiesel and lipid product production.

Data-Driven Modeling and Operation Optimization With Inherent Feature Extraction for Complex Industrial Processes

In response to the tenets of Industry 4.0, operation optimization in industrial processes has become a significant research topic. However, the uncertainties prevailing in the process pose challenges to production operations, especially the feedstock properties. In this work, the operation optimization study is performed on a distillation unit (DU), a typical plant in the industrial process. To enhance production performance, a modeling and operation optimization strategy based on feedstock property and production features is presented. One of the difficulties is how to uncover features from high-dimensional and imperfect data, where imperfect data refers to product quality data that is unavailable online. In the strategy, we inject the inherent characteristic of the process into the data-driven method to extract the feedstock property in a data-based and knowledge-oriented manner. Further, optimal feature representation and process modeling can be achieved by customizing the network structure. The operation optimization problem is formulated to adjust the top temperature of the distillation column (TTDC) to achieve satisfactory production under varying feedstock properties. Experimental results illustrate that the process model based on feedstock property and production features (PM-FP-PF) can better fit the physical process mechanism even based on incomplete information in industrial data. Industrial experiments have shown the proposed strategy has advanced generalization ability to the different feedstock properties. The proposed operation optimization strategy (OOS) improves the product qualification rate and has broad application prospects in industrial processes with similar features. <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Note to Practitioners</i> —Industrial processes suffer from a variety of disturbances that interrupt the smooth operation of the system, such as varying feedstock properties. How to deal with them is the key to improve the product qualification rate. In this work, we propose a data-driven modeling and operation optimization framework to improve product quality under varying feedstock properties. The dynamic variation characteristics of the feedstock properties can be obtained by analyzing the physical properties of the production unit. This is used as a basis for representing and extracting feedstock properties in a data-driven way. Further, a process model based on feedstock property and production features (PM-FP-PF) is built to predict product quality. An operation optimization strategy with production capacity consideration is established. It can determine the optimal operation action required by the current system, mitigating the uncertainty of feedstock property. This operation optimization system has been applied to a distillation unit in the hydrofining process. The application results show that the process model achieves satisfactory estimation accuracy, and the operation optimization strategy has improved production performance.

Selective extraction of potassium from raw nepheline materials

The global aluminium industry is ever-changing and primarily relies on bauxite as its traditional source. However, due to the finite reserves of processed bauxite, alternative sources need to be found. Nephelines have emerged as potential alumina sources. In addition to alumina, nepheline processing can yield marketable products such as soda, potash, cement, and rare metals.The objective of this study was to identify the ideal conditions for selectively extracting potassium from alkaline leaching solutions while separating it from sodium alkali, aluminium, and silicon during the comprehensive processing of nepheline syenites from the Kubasadyr deposit. This approach involved a two-stage hydrothermal leaching process with an incremental calcium addition. Under optimal conditions, the potassium extraction in the alkaline solution reached 93.91%. The resulting leaching solution served as a feedstock for potassium sulfate production through the crystallization method. The preliminary selective separation of potassium played a crucial role in mitigating its adverse effects on the extraction of aluminium, which is the primary processed product; thus, the economic viability of production was enhanced.

Electron Beam on Fermentation Medium as an Alternative Disinfection Method for Ethanol Distilleries: A Comprehensive Review

Corn and sugarcane are the primary feedstocks for ethanol production, but microbial contamination hinders yeast fermentation efficiency. Current control methods include antibiotics and sulfuric acid, but they have limitations, resulting in dependence on external inputs and the risk of antibiotic-resistant bacteria. This review examines electron beam technology as an industrial-scale disinfection solution for both corn- and sugarcane-based ethanol production, highlighting its advantages, limitations and opportunities for adoption in Brazil. A critical evaluation highlights the importance of optimal operating conditions for scalability, cost effectiveness and sustainable implementation. Through a practical example, we demonstrate the effectiveness of electron beam treatment in improving fermentation efficiency and reducing contamination-related losses. Notably, the ionizing radiation from this process does not affect wort sugar content or generate radioactive residues. While acknowledging the potentially high energy input requirements, cogeneration in sugarcane mills can address this, making it a viable option; however, further technical and economic evaluation should be made. In addition, electron beam technology is a promising approach for the production of high-value products such as neutral alcohol, amino acids, animal feed and pharmaceuticals. Therefore, this comprehensive review provides valuable insights for researchers, industry stakeholders and policymakers to promote sustainable and efficient ethanol production practices.

A two-step method of cyclolinopeptide (linusorb) preparation from flaxseed cake via dry-screening

Linusorbs (LO), cyclolinopeptides, are a group of cyclic hydrophobic peptides and considered a valuable by-product of flaxseed oil due to numerous health benefits. Currently applied acetone or methanol extraction could contaminate the feedstocks for further food-grade application. Using flaxseed cake as feedstock, this study established a practical method for preparing LO from pressed cake. Firstly, LO composition of 15 flaxseed cultivars was analyzed. Next, cold-pressed cake was milled and screened mechanically. The kernel and hull fractions were separated based on the disparity of their mechanical strength. Monitored by hyperspectral fluorescence, the LO-enriched kernel fraction separated from cold-pressed flaxseed cake was further used as feedstock for LO production. After ethanol extraction, partition, and precipitation, LOs were extracted from cold-pressed flaxseed cake with a purity of 91.4%. The proposed method could serve as feasible flaxseed cake valorization strategy and enable the preparation of other polar compounds such as flax lignan and mucilage.

Mutation breeding of high-stress resistant strains for succinic acid production from corn straw

The production of succinic acid from corn stover is a promising and sustainable route; however, during the pretreatment stage, byproducts such as organic acids, furan-based compounds, and phenolic compounds generated from corn stover inhibit the microbial fermentation process. Selecting strains that are resistant to stress and utilizing nondetoxified corn stover hydrolysate as a feedstock for succinic acid production could be effective. In this study, A. succinogenes CICC11014 was selected as the original strain, and the stress-resistant strain A. succinogenes M4 was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis and further screening. Compared to the original strain, A. succinogenes M4 exhibited a twofold increase in stress resistance and a 113% increase in succinic acid production when hydrolysate was used as the substrate. By conducting whole-genome resequencing of A. succinogenes M4 and comparing it with the original strain, four nonsynonymous gene mutations and two upstream regions with base losses were identified.Key points• A high-stress-resistant strain A. succinogenes M4 was obtained by ARTP mutation•  The production of succinic acid increased by 113%• The mutated genes of A. succinogenes M4 were detected and analyzedGraphical