Fish Feed Research Articles

Gene Expression Study in Gilthead Seabream (Sparus aurata): Effects of Dietary Supplementation with Olive Oil Polyphenols on Immunity, Metabolic, and Oxidative Stress Pathways

In an era with an ever-growing population, sustainability and green transition are the main milestones to be considered within the current European Green Deal program, and the recovery of by-products for the integration of feed with bioactive molecules, that are sustainable and with high nutritional value, is an ambitious mission to be explored also in aquaculture. Olive oil extraction produces a range of solid and liquid by-products, in varying proportions depending on the utilized production techniques, all of which are considered as possible pollutants. However, these products are also rich of polyphenols, bioactive molecules with several and well-known beneficial properties (antimicrobic, anti-inflammatory, antioxidant, and immune-modulating). On this basis, this work aimed at evaluating the effects of dietary supplementation with polyphenols derived from olive mill wastewater on growth performance and on gene expression modulation, by means of RT-qPCR assays, in farmed Sparus aurata. Particularly, some target genes of metabolic, immunity, and oxidative stress pathways have been investigated in breeding gilthead seabream. Differential gene expression analysis was carried out, and differences between the control group (n = 9) and the treated one (n = 9) were computed with Student’s t test. The results have highlighted that supplemented feed enhanced fish growth, with a significant feed conversion ratio between the two groups. Furthermore, the polyphenol diet had a beneficial impact on gene expression fold with a level of significance for fatty acid binding protein 2, superoxide dismutase 1, and interleukin-12 genes at hepatic or intestinal district. These significant and promising preliminary findings promote, in the future, other investigations on polyphenolic by-products and on their putative or possible re-utilization in fish feeding.

Nourishing Aquatic Life: A Comprehensive Reviewing of Fish Feed Strategies and Disease Combat in Aquaculture

With an increasing focus on creating sustainable methods that satisfy the nutritional requirements of aquatic life while successfully containing disease outbreaks, aquaculture has emerged as a crucial element of global food security. Nourishing Aquatic Life: A Comprehensive Review of Fish Feed Strategies and Disease Combat in Aquaculture explores the delicate balance between disease management and feed optimization, two crucial factors influencing the ecological sustainability and productivity of aquaculture systems. Innovative substitutes, including plant-based proteins, insect meal, and microbial sources, are being assessed alongside conventional fish feed formulations, which rely on fishmeal and fish oil to lessen environmental stresses while preserving high nutritional value. This article also covers how probiotics, prebiotics, and immunostimulants can improve fish immunity and resilience against common diseases, lowering the need for antibiotics and the likelihood of resistance. Additionally, we look at the most recent developments in fish feed formulation using biofloc, genetic engineering, and nanotechnology, emphasizing their implications for better fish health and disease resistance. These innovative strategies offer a hopeful vision for the future of aquaculture, inspiring us to continue our efforts in this field.

Evaluation of Ethanolic Extract of Red Seaweed (Gracilariopsis lemaneiformis) on Growth and Haematological Parameters of Nile Tilapia (Oreochromis niloticus)

ABSTRACTSeaweed is multicellular marine macroalgae that enhances the immunity of aquatic organisms. The study was designed to assess the ethanolic extract (EtOH) extracted from red seaweed (Gracilariopsis lemaneiformis) on Nile tilapia (Oreochromis niloticus) to evaluate growth, immunity and haematological parameters. The EtOH was applied with fish feed in four treatments such as T1‐control (0% EtOH), T2 (5% w/v EtOH), T3 (10% w/v EtOH) and T4 (15% w/v EtOH) each with three replicates. The initial average weight of the fish was 17.53 ± 2.1 g. Results showed that the growth increased gradually with the percentage of red seaweed extract, whereas the mean weight gain was found significantly higher in T4 (51.5 ± 4.2) than in the control. The specific growth rate (%) and survival rate (%) were found higher in T4 at the lowest FCR compared to other treatments (p < 0.05). The blood haemoglobin level of Nile tilapia was higher in T4 (11.5 g/dL) and lowest recorded in T1 (7.5 g/dL). However, the level of blood glucose interacted inversely with blood haemoglobin. The plasma protein level was also varied among the treatments (p > 0.05). The blood parameters, refractive index (RI) and spleen somatic index (SSI) were significantly different among the treatments. The RI of blood plasma and SSI were found to be better in T4 treatment. The research indicated that red seaweed extracts significantly enhanced the growth and boosted the immunity level of Nile tilapia.

Detection of Bombyx mori as a Protein Source in Feedingstuffs by Real-Time PCR with a Single-Copy Gene Target

The silkworm, Bombyx mori, is reared on a large scale, mainly for silk production. The waste from this silk production, like pupae, is underused. As an edible insect, B. mori is a good source of protein in human food and animal feed. In recent years, European legislation on the use of insects has evolved and a multitude of European companies have initiated the rearing of insects specifically for food and feed applications. Regarding animal feed, Commission Regulations (EU) 2021/1372 and 2021/1925 authorize eight insect species, including silkworm, as processed animal proteins for use in fish, pig, and poultry feed. The incorporation of edible insects into the human diet falls within Regulation (EU) No. 2015/2283 concerning novel foods. Implementation of authentication methods is imperative to ensure the conformity of the products. In the present study, we propose a specific real-time PCR method for the detection of silkworm (B. mori). The developed PCR test amplifies a 98 bp fragment of the cadherin gene. This gene is present in a single-copy per haploid genome, as demonstrated by experimental evidence. The qualitative method was successfully evaluated on the performance criteria of specificity, sensitivity, efficiency, robustness, and transferability. The applicability of the test was assessed on samples of B. mori from industry. Light microscopy and DNA metabarcoding approaches were used as a complement to genomic analysis as a means of providing authentication of the samples.

Exploring the lipids, carotenoids, and vitamins content of Rhodotorula glutinis with selenium supplementation under lipid accumulating and growth proliferation conditions

BackgroundRhodotorula glutinis, a specific type of yeast, has been recognised as a superior resource for generating selenium-enriched biomass that possesses exceptional nutritional and functional attributes. The purpose of this investigation was to assess the effect of sodium selenite at different concentrations on lipid and carotenoid synthesis, as well as the growth of R. glutinis.MethodsThe lipid’s fatty acid composition was determined using gas chromatography (GC). The vitamins were detected by high-performance liquid chromatography (HPLC). Transmission electron microscopy was used to detect the structural modification of yeast cells caused by the addition of sodium selenite to the growth medium, as well as the accumulation of elemental selenium in the yeast cells.ResultsThe yeast cells demonstrated the ability to endure high concentrations of sodium selenite under lipid accumulation (LAM) and growth-promoting (YPD) conditions. 25.0 mM and 30.0 mM, respectively, were published as the IC50 values for the LAM and YPD conditions. In both growth media, 1 mM sodium selenite boosted lipid synthesis. Lipid accumulation increased 26% in LAM to 11.4 g/l and 18% in YPD to 4.3 g/l. Adding 1 mM and 3 mM sodium selenite to YPD medium increased total and cellular carotenoids by 22.8% (646.7 µg/L and 32.12 µg/g) and 48.7% (783.3 µg/L and 36.43 µg/g), respectively. Palmitic acid was identified as the most abundant fatty acid in all treatments, followed by oleic acid and linoleic acid. The concentrations of water soluble vitamins (WSV) and fat soluble vitamins (FSV) were generally significantly increased after supplementation with 1.0 mM sodium selenite. TEM examination revealed a significant reduction in lipid bodies accumulation in the yeast cells when sodium selenite was added to lipid-promoting environments. This decline is accompanied by an augmentation in the formation of peroxisomes, indicating that selenium has a direct impact on the degradation of fatty acids. In addition, autophagy appears to be the primary mechanism by which selenium ions are detoxified. Additionally, intracellular organelles disintegrate, cytoplasmic vacuolization occurs, and the cell wall and plasma membrane rupture, resulting in the discharge of cytoplasmic contents, when a high concentration of sodium selenite (20.0 mM) is added. Also, the presence of numerous electron-dense granules suggests an intracellular selenium-detoxification pathway.ConclusionThis study proposes the use of YPD with 1 mM sodium selenite to cultivate selenium-enriched biomass from R. glutinis. This approach leads to heightened lipid levels with higher accumulation of oleic, linoleic and linolenic acids, carotenoids, and vitamins. Hence, this biomass has the potential to be a valuable additive for animal, fish, and poultry feed. Furthermore, explain certain potential factors that indicate the impact of selenium in reducing the accumulation of lipid droplets in R. glutinis during lipogenesis, as detected through TEM examination.

Optimizing Fish Feed Pellets Handling Systems to Minimize Microplastics Emissions - A Pilot Test Based Approach

The fisheries and aquaculture industries are vital components of global food production, yet they also contribute to release of microplastics into marine environments, posing risks to ecosystem health and seafood safety. Among the contributing factors, the erosion of feeding pipes during the pneumatic conveyance of fish feed pellets stands out as a significant source of microplastic pollution. This study focuses on optimizing feed pellet conveying systems in fish farms to minimize microplastic emissions while maximizing pipeline lifespan and pellet integrity. Using high-density polyethylene (HDPE) pipes commonly found in aquaculture facilities, the impact of air velocity and pipeline configuration on pipe wall erosion and pellet breakage was investigated. Through pneumatic conveying tests, the effects of varying air flow rates and bend radii were assessed on pipeline wear and feed pellet integrity. The findings of the study underscored the importance of optimizing operating parameters to bring a balance between preventing pipe blockages and minimizing abrasive impacts on pellets and pipeline surfaces. Furthermore, a simulationbased approach to optimize feeding system performance is presented, integrating the experimental results into a computational model. This model allows for the evaluation of different operating conditions and pipeline configurations, offering insights into costeffective strategies for reducing microplastic emissions while maintaining efficient feed delivery to fish populations. Ultimately, this research provided practical recommendations and best practices for the aquaculture industry to mitigate microplastic pollution from feeding pipes. By optimizing feed pellet conveying systems, environmental sustainability can be enhanced, seafood quality be preserved, and bolster consumer confidence in aquaculture products.

Biosynthesis of gold nanoparticles and its impacts on striped dwarf catfish (Mystus vittatus) as feed additives

The study evaluated the impacts of gold nanoparticles (AuNPs) on striped dwarf catfish’s (Mystus vittatus) growth, survival, and hematological parameters. Gold nanoparticles were synthesized using lemon (Citrus limon) extract, and four experimental diets incorporating 0, 10, 30, and 50 mg of AuNPs per Kg of fish feed were prepared as treatments T0 (control), T1, T2, and T3, respectively. Forty-five days old (0.7±0.00 g) 60 fingerlings were stocked in each glass aquarium and the diets were administered daily for 75 days. The treatment T2 resulted in a significant (P<0.05) increase in growth performances, feed conversion ratio, protein efficiency ratio, and survival rate. Besides, significant (P<0.05) differences were found in hematological parameters such as red blood cell count, hemoglobin level, and white blood cell count in the T2 treatment. However, a significant decrease in the white blood cell counts was observed in T3 treatment. Improved histological condition of hepatocytes was noticed in all treatments compared to the control. Hence, the results from this study suggest that the incorporation of 30 mg/Kg of biosynthesized AuNPs in the diet could be a promising strategy for enhancing the growth, survivability, and hematological parameters of M. vittatus.

Macrophytes Used as Multifaceted Benefits Including Feeding, Bioremediation, and Symbiosis in Freshwater Aquaculture—A Review

ABSTRACTThe utility of macrophytes in freshwater aquaculture is diverse. We review the benefits of macrophytes in aquaculture into broadly three useful perspectives: (i) Feeding, (ii) Bioremediation, and (iii) Symbiosis. Macrophytes are used as direct food by herbivores for their potential nutrient values with a maximal protein content of 35% (D/W basis) and as ingredients for fish feed preparation with optimal 50% protein inclusion to reduce feed cost in commercial aquaculture. Macrophytes are used for wastewater treatments to eliminate contaminants, including As, Cu, Cd, Cr, Hg, and Pb, to 99.9%, and to reduce BOD (Biological Oxygen Demand) to improve water quality. Intake of remedied wastewater into aquaculture enhances water productivity through phytoplankton growth. An estimate is that 1.0 L of remedied wastewater, particularly sewage effluent adds an average of 0.05 g N &amp; P and 0.99‐L freshwater into aquaculture to yield an average of 0.309 g fish. Macrophytes are integrated with fish in commercial aqua‐farming. Plants and fish are farmed together in aquaponics. They are used as substrates for some commercial fish species to lay eggs in farming. They are raised with ornamental fish for benefits in aquariums. Nevertheless, extensive studies are required to harness more benefits from macrophytes.

Impacts of alfalfa nutrient concentrate on pellet physical attributes, growth performance, metabolism and nutritional quality of rainbow trout, Oncorhynchus mykiss

This study addresses the escalating demand for aquatic feed by exploring the potential of alfalfa nutrient concentrate (ANC) as feed ingredient for rainbow trout. Test diets contained varying ANC levels (0%, 5%, 10%, 15%, and 20%) to replace fishmeal (32% in the 0% ANC diet) to achieve equal digestible protein and were processed using a cooking extrusion method. Analysis of feed pellets showed that pellet density increased with ANC levels (P < 0.001), resulting in sinking pellets at 20% ANC. Water stability and durability were improved while oil leakage decreased with increasing ANC levels (P < 0.05). Two feeding trials were conducted to test the diets in flow-through water systems with three replicates per diet. The first 10-week trial evaluated their impact on feeding, fecal physical quality, and the apparent digestibility coefficient (ADC) of dietary nutrients in rainbow trout (initial body weight , 18.0 ± 0.2 g). ANC inclusion did not impact the palatability and satiety feed intake of the fish (P > 0.05). However, the ADC of dry matter and phosphorus significantly decreased in fish fed the 20% ANC diet (P < 0.05). The second 9-week trial investigated the growth performance, nutrition quality, and metabolism of rainbow trout (initial body weight, 19.0 ± 0.2 g). While all fish exhibited substantial growth, fish fed diets with 10% to 20% ANC displayed lower specific growth rate and higher feed conversion ratio compared to those fed with 0% or 5% ANC (P < 0.05). The whole body protein content was higher in fish fed 5% ANC compared to all other treatments (P = 0.030). The biochemical parameters of plasma were similar across treatments, except for a decrease in plasma phosphorus levels in fish fed a 10% ANC diet compared to those fed a 0% ANC diet. (P = 0.033). Significant changes were observed in liver metabolism including tricarboxylic acid cycle, amino acid and energy metabolism pathways in fish fed the 20% ANC diet versus the 0% ANC diet (P < 0.05). These results demonstrate that ANC inclusion improved pellet physical quality without impairing feeding behavior and nutritional quality of the fish but inclusion ≥10% in the diet reduced fish growth. This study offers the first comprehensive assessment of the potential of ANC used in fish feed involving feed management, feeding evaluation, and the biological response.

Dual Function of Sea Grapes (Caulerpa racemosa) As Phytoremediator for Palm Oil Mill Effluent and As Ornamental Fish Feed Formulation.

Phytoremediation is a promising technology for treating Palm Oil Mill Effluent (POME). Moreover, phytoremediators have the potential for various aplication, including as feedstock. Hence, this study aims to elucidate the ability of sea grapes (Caulerpa racemosa) in remediating POME and evaluate their suitability as ornamental fish feed. Results showed that application of sea grapes effectively decreased the COD, TSS, phosphate (PO43-), and nitrate (NO3-) levels in POME. Sea grapes maintained in POME with a concentration of 12.5% had the highest reduction rate and growth performance. Moreover, sea grapes biomass from the remediation process can be utilized as feed material for ornamental fish, as indicated by increasing skin coloration of fish. For the first time, this study provides sustainable options for managing POME using sea grapes and suggests sea grapes as a potential fish feed formulation for ornamental fish.

Automation System of Water Circulation and Fish Feeding in Tilapia Fish Pond with Solar Panel Source

Tilapia cultivation requires good management of water quality and proper feeding to ensure optimal fish growth. One of the main challenges in this process is maintaining the water pH level within ideal limits and providing feed according to the needs of the fish based on their size. In addition, limited access to electricity in some locations makes fish pond management less efficient. This study aims to design an automated water circulation system that is regulated based on the water pH level and automatic feeding according to the needs of the fish, by utilizing solar panels as a power source. This system uses a pH sensor to monitor water conditions in real time and regulate water circulation to maintain ideal pH levels. Feeding is regulated based on the size and number of fish to ensure optimal feed efficiency and fish growth. This system is powered by solar panel energy, so it can operate independently without relying on conventional electricity networks. Test results show that the system is able to maintain water pH stability and automate feeding with high accuracy, while utilizing renewable energy effectively. The use of solar panels not only reduces dependence on electricity, but also reduces operational costs and supports environmental sustainability. This system is expected to increase the efficiency and productivity of tilapia cultivation, especially in areas with limited access to electricity.

Perancangan Sistem Akuntansi Persediaan Pakan Ikan Berbasis Google Spreadsheet pada Usaha Budidaya Perikanan

This study aims to design a fish feed inventory record at UD Bless using Google Spreadsheets. The research method is qualitative. Primary data in this study were obtained by conducting direct interviews with business owners. Secondary data in this study are in the form of transaction evidence, inventory records arranged in archives. The results of the study are the design of a feed inventory accounting system at UD Bless which consists of a list of feed inventory, inventory purchases and sales, cash and credit inventory purchases, cash and credit inventory sales, and profitability of each type of inventory.

Application of Biofloc Techznology as an Environmentally Friendly Solution to Increase the Productivity of Freshwater Fish Cultivation

This study explores the application of Biofloc Technology (BFT) as an environmentally friendly solution to increase the productivity of freshwater fish cultivation. Using a qualitative research approach based on a comprehensive literature review and library research, the paper examines the effectiveness of Biofloc in improving water quality, reducing feed costs, and enhancing fish growth rates. BFT promotes the growth of beneficial microbial communities that convert organic waste into protein-rich bioflocs, which serve as a supplementary feed for fish. This technology minimizes water exchange, conserves resources, and mitigates environmental pollution in aquaculture systems. The study highlights various successful implementations of Biofloc in freshwater aquaculture, noting significant improvements in production efficiency and sustainability compared to traditional farming methods. Moreover, the paper discusses the economic and environmental benefits of adopting Biofloc Technology, including reduced dependency on high-cost commercial feed and lower environmental impact due to decreased water usage and waste output. The findings suggest that Biofloc Technology is a promising innovation for sustainable aquaculture, providing both ecological and economic advantages. This research provides insights for policymakers, fish farmers, and aquaculture industry stakeholders interested in adopting eco-friendly practices to enhance productivity while protecting aquatic ecosystems

Effect of the combination of two types of fish feed with nutritional quality on the performance of two species of fish raised on rural and peri-urban farms in Cote D'ivoire

For 160 days, juvenile Oreochromis niloticus and Heterobranchus longifilis were in ponds on a farm in San Perdo located in the southwest of Côte d’Ivoire.The juveniles grouped in four lot per species and in triplicate were fed in pairs of industrial feed and feed produced on the farm and an agri-feed by-product from four feeding techniques (Batch 1: juveniles fed with an industrial lime in the pre-growing phase followed by an agricultural by-product in the growing phase. Batch 2: Juveniles fed with an industrial feed then a feed formulated with an agricultural product during the growing phase ; Batch 3: juveniles fed with an agricultural by-product in the pre-growing phase followed by an industrial feed in the growing phase. Batch 4: Juveniles fed with a formulated feed in the pre-growing phase then with an industrial feed in the growing phase). At the end of the experiment, the best zootechnical parameters were obtained whatever the species by the juveniles fed in the pre-grossing phase with a produced feed followed by industrial feed in the production growing phase.

An Investigation on the Food and Feeding Habit of &amp;lt;i&amp;gt;Sarotherodon Galilaeus &amp;lt;/i&amp;gt;(Cichlidae) in Egbe Reservoir, Ekiti State, Nigeria

A study on the food and feeding ecology of &amp;lt;i&amp;gt;Sarotherodon galilaeus&amp;lt;/i&amp;gt;, a species of cichlid found in Nigeria&amp;apos;s Egbe Reservoir. For the investigation, 305 fish specimens were used. The stomach contents were analyzed using diet indices such as stomach fullness, numerical approach, and frequency of occurrence method. Additionally, the Geometric Index of value (GII) was used to determine the relative value of these food products. The findings showed that both adult and juvenile &amp;lt;i&amp;gt;Sarotherodon galilaeus&amp;lt;/i&amp;gt; fed on nine various food sources. The variety of food items included rotifers, algae, desmids, diatoms, protozoa, detritus, aquatic insects, aquatic plants, and insect larvae. The inclusion of detritus in the food items suggests that the fish species graze on both surface water and bottom sediments. Algae had the highest frequency of occurrence (46.39%) in both sizes and seasons, whereas rotifer had the lowest value (1.55%). Since it is possible to create the artificial diet required for this species&amp;apos; mass production in Nigeria, the findings of this study are very significant for the culture of that species&amp;lt;i&amp;gt;. Sarotherodon galilaeus&amp;lt;/i&amp;gt; can be classified as an omnivore because it consumed a variety of foods. Since feeding accounts for 60–65% of the aquaculture industry for any species being raised, research on fish diet and feeding habits is essential to developing an effective aquaculture management system. As a result, the fish species may be raised in our garden.

Enhancing Aquaculture Productivity through Integrated Farming Systems: A Comparative Study of Fish-Pig and Fish-Duck Systems

Integrated farming systems involving fish-animal are of high relevance in current scenario. Studies like this asses the suitability of such systems in improving the aquaculture productivity and overall benefits to the ecosystem. It plays a crucial role in optimizing the use of small water resources in rural areas to enhance animal production. Given the potential of pig and duck manure as nutrient inputs in fish culture, a grow-out trial was conducted in a 0.1 ha earthen pond located in Elangitampatty, Kezhisenkattupatti, and Asakkattuppatti villages of Kollihills Taluk in Namakkal district, Tamil Nadu. The pond was stocked with four carp species: Catla, Rohu, Mrigal, and Grass carp in a ratio of 40:20:30:10 at a density of 7500 fish per hectare. Three treatments were employed: (1) Control (T0), where fish were fed with commercial feed, (2) Integrated fish-duck farming (T1), and (3) Integrated pig-fish farming (T2). In the control group (T0), fish were fed commercial fish feed (24% crude protein, 4 mm pellet size) at 4% of body weight, while in T1 and T2, fish received 50% of the commercial feed and were supplemented with duck and pig excreta, respectively. After an 8-month grow-out period, fish weights and total production in each group were recorded. Water quality parameters, such as pH, dissolved oxygen, free carbon dioxide, alkalinity, and hardness, were measured using standard methods (APHA, 2019). Results showed that pond fertilization with pig and duck excreta promoted the growth of algae, phytoplankton, and zooplankton, providing a natural food source for the fish. Total fish production was highest in the fish-pig integration system (T2), with a recorded yield of 4960.09 kg. Catla (Catla catla) had the highest individual species yield, with a total harvested weight of 2225.98 kg, followed by Grass carp (Ctenopharyngodon idella) at 1490.37 kg. The final average weight of Grass carp in T0, T1, and T2 was 1047 ± 13.27 g, 1151.10 ± 12.85 g, and 1490.37 ± 13.52 g, respectively. For Catla, the final average weight in T0, T1, and T2 was 910 ± 9.45 g, 960 ± 10.26 g, and 980 ± 12.20 g, respectively. Similarly, Rohu in T0, T1, and T2 weighed 850 ± 7.98 g, 900 ± 11.23 g, and 910 ± 11.16 g, respectively, while Mrigal in T0, T1, and T2 weighed 600 ± 6.04 g, 720 ± 8.50 g, and 730 ± 10.56 g, respectively. The results highlight that the fish-pig system outperformed the fish-duck system in terms of fish productivity, which may be due to the better nutrient composition in pig manure and the genetic potential of the fish species. An economic analysis revealed that the fish-pig integration system generated the highest net income (Rs. 555,353.90) and benefit-cost ratio (2.12), indicating that integrated fish-pig farming is more profitable than both fish-duck farming and the control group without integration.

Rancang Bangun Pakan Ikan Otomatis dan Kontroling Suhu Serta Monitoring pH Air Berbasis IoT

This research proposes the design of an automatic fish feeding system equipped withtemperature control and water pH monitoring based on the Internet of Things (IoT). Thissystem aims to increase efficiency in aquarium or fish pond management by integratingvarious control and monitoring functions in real-time. In this system, the automatic fishfeeder uses a programmable dispenser mechanism to provide feed in predeterminedamounts and times. Temperature control is carried out using temperature sensors andheating or cooling actuators, to maintain water temperature conditions within the optimalrange. In addition, water pH monitoring is carried out through a pH sensor connected tothe system to ensure the water quality remains ideal for fish health. Data from these sensorsis sent in real-time to the IoT platform, enabling remote monitoring and control via webbasedor mobile applications. The results of implementing this system show increasedefficiency in feeding, increasing operational efficiency, reducing human error, andproviding quality fish, as well as maintaining more stable and optimal environmentalconditions. The implementation of this system shows great potential in modern aquacultureapplications by utilizing IoT technology for better and responsive management.

The Box Crab Calappa hepatica as a Nuclear Species for the Opportunistic Foraging Behaviour of the Flowery Flounder, Bothus mancus, in the Indo-Pacific

Some predatory fishes may exhibit opportunistic feeding behaviour by exploiting potential prey that is distracted, displaced, or exposed by the activities of a third party that acts as a ‘nuclear’ species. Other fishes mostly perform the role of ‘nuclear’ species, but benthic invertebrates, such as octopuses, have also been reported. Crabs are rarely observed in this role, with only a few records from the tropical Atlantic Ocean. Here, we report the temporary association between two specimens of the flowery flounder, Bothus mancus (family Bothidae), and a box crab, Calappa hepatica (family Calappidae), from the Philippines, representing the first record of a crab–fish feeding association in the Indo-Pacific region.

Effect of Different Purities of Seed Cells and Culture Media on the Growth Pattern and Protein Content of Spirulina Platensis

Spriulian platensis is a type of microalgae commonly used as natural fish feed. The purity of cell seedlings represents an internal factor, while the culture media acts as an external factor that can limit microalgae growth. This research investigates how varying purities of cell seedlings and culture media affect the growth patterns and protein content of Spriulian platensis, as well as to identify the optimal purity of cell seedlings and culture media that best promote growth patterns and protein content. The research method employed was an experimental design using a completely randomized factorial design with four treatment combinations and three replicates. The results showed that factor A (purity of seed cells) significantly affected growth patterns, including the lag phase duration, specific growth rate, and maximum density, but did not influence the final density Spriulian platensis. Factor B (culture media) had no significant effect on any of the variables. The combination of axenic seedlings with Pro Analis media was identified as the best treatment, producing a lag phase duration of (-4.17 ± 0.03 cells/day), a growth rate of (0.33 ± 0.01 cells/day), a maximum density of (6.43 ± 0.02 log cells/ml), and a final density of (5.78 ± 0.03 log cells/ml). The protein content across all treatments remained consistent at 58-60%. The study concludes that the purity of cell seedlings is a critical factor in determining the growth pattern of Spirulina platensis, particularly affecting the lag phase, growth rate, and maximum density.

Predicting Tilapia Productivity in Geothermal Ponds: A Genetic Algorithm Approach for Sustainable Aquaculture Practices

This study presents a case focused on sustainable farming practices, specifically the cultivation of tilapia (Mozambican and aureus species) in ponds with geothermal water. This research aims to optimize the hydrochemical regime of experimental ponds to enhance the growth metrics and external characteristics of tilapia breeders. The dataset encompasses the hydrochemical parameters and the fish feeding base from experimental geothermal ponds where tilapia were cultivated. Genetic algorithms (GA) were employed for hyperparameter optimization (HPO) of deep neural networks (DNN) to enhance the prediction of fish productivity in each pond under varying conditions, achieving an R2 score of 0.94. This GA-driven HPO process is a robust method for optimizing aquaculture practices by accurately predicting how different pond conditions and feed bases influence the productivity of tilapia. By accurately determining these factors, the model promotes sustainable practices, improving breeding outcomes and maximizing productivity in tilapia aquaculture. This approach can also be applied to other aquaculture systems, enhancing efficiency and sustainability across various species.