Feeding Inulin Research Articles

Effects of inulin on fecal microbiota and specific immunity in cats

Inulin has potential benefits for alleviating intestinal stress syndrome, constipation, and immunomodulation. However, its effects on cat gastrointestinal tract remain unexplored. Eight healthy adult British short-haired cat were administered 50 mg/kg/d inulin with a basal diet for 21 days, while fecal samples were collected to measure indole and 3-methylindole levels, immune index detection, and fecal microbial diversity on days 0, 7, 14, and 21. The results showed that adding inulin to the diet of cat could cause the increase of sIgA on day 14 (P < 0.05) and enhance their immune performance. In addition, it will also affect the fecal microbiota of the cat. Collinsella abundance was significantly increased, which could indulge ursodeoxycholic acid production. Feeding inulin had no significant effect on the levels of indole and 3-methylindole (P > 0.05). The above results showed that inulin supplementation in cat diet could improve cat health by enhancing immunity and increasing intestinal beneficial flora.

Inulin supplementation increases the differential metabolites and metabolic pathway in Baird's tapirs (Tapirus bairdii).

The prevalence of prebiotic has increased substantially over the past decades. Little is known on its metabolic effects in zoo herbivores. We investigated the difference in faecal metabolites to characterize the composition and pathways involved after feeding inulin in zoo Baird's tapirs (Tapirus bairdii). Faecal samples were collected from before inulin treatment group and after treatment groups in six adult tapirs and analysed using untargeted liquid chromatography-mass spectrometry methods. The differential metabolites identified and metabolic pathways involved were analysed using KEGG annotation. The results demonstrated significant alterations in faecal metabolites and metabolic pathways in comparison to the control group. The amounts of differential metabolites and metabolic pathways tended to enrich with time after the treatment. We found that tryptophan and purine metabolism were relevant to the important metabolic pathways of the metabolite differences. The findings suggest that inulin may have potential applications in captive wildlife, which may provide insights into the effects of prebiotic supplementation on gut metabolites and highlights further research in this field.

Feeding reduced dietary protein added with prebiotics on protein metabolism characteristics and growth performance of broiler chickens

The purpose of the present study was to evaluate the effect of feeding prebiotics on protein metabolism characteristics and growth performance of broilers. A completely randomized design with 4 treatments and 5 replications was arranged. Heterophil/lymphocyte (H/L) ratio, protein metabolism characteristics (protein digestibility, fecal Nt-MH excretion, and meat protein mass), and growth performances (feed consumption, BWG, and FCR) were the parameters observed. Analysis of variance was performed to statistically evaluate the data, and continued to Duncan test at 5% probability. Protein digestibility, and meat protein mass in T1 and T2 were significantly (P<0.05), while those in T0 were significantly (P<0.05). On the contrary, H/L ratio, feed consumpton, and FCR were significantly (P<0.05) lowest in both T1 and T2, but those in T0 were significantly (P<0.05). In conclusion, feeding inulin and soy-oligosaccharide extracts improves protein metabolism and it is characterized by the increase in protein digestibility and meat protein mass, and promote better growth of broiler.

Effects of dietary fibres on gut microbial metabolites and liver lipid metabolism in growing pigs.

This study was conducted to investigate the effects of dietary supplementation with wheat bran fibre, inulin and their combination on growth performance, short-chain fatty acids (SCFAs) production in caecum and colon and liver lipid metabolism in growing pigs. A total of 48 Duroc×Landrace×Yorkshire cross-bred growing pigs (73±2days of age; 24.37±2.86kg) were allocated to four groups randomly, each group consisting of six pens with two pigs each. The pigs were fed a control diet (CON), a diet containing 2% wheat bran fibre (WB), a diet containing 2% inulin (IN), and a diet containing both of 1% wheat bran fibre and 1% inulin (MIX), respectively. The trial lasted for 28days. The results showed that MIX fed pigs had a higher percentage of fat in the liver than those fed the CON (p<.05). IN, WB or MIX feeding decreased the concentrations of acetate and total SCFAs in colon compared with CON (p<.01). Feeding WB or IN also decreased the colonic butyrate concentrations compared with CON (p<.01). However, the serum level of valeric acid was elevated in the IN, WB and MIX group (p<.01). MIX fed pigs tended to have lower levels of propionate in serum than the WB fed pigs (p=.098). MIX feeding enhanced the mRNA expression of lipid synthesis-related genes in liver compared with CON (p<.05). Feeding IN decreased the expression of bile acids synthesis-related genes in liver and increased mRNA expression of SCFAs transporter SLC16A1 in colon compared with CON (p<.05). In this study, these data indicated that the combined supplementation of wheat bran fibre and inulin decreased the SCFAs concentrations in the colon, enhanced the genes FAS and HNF-4α mRNA expression in liver and induced liver lipid accumulation in growing pigs.

Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance

In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance. At day 80 of gestation sows were randomly assigned to four groups：basal diet (CON); or basal diet with 0.8 %; 1.6 %; or 2.4 % inulin. The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05). When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05). Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05). Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05). Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05). In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.

Inulin supplementation reduces the negative effect of a high-fat diet rich in SFA on bone health of growing pigs.

Consumption of a high-fat diet, rich in SFA, causes deterioration of bone properties. Some studies suggest that feeding inulin to animals may increase mineral absorption and positively affect bone quality; however, these studies have been carried out only on rodents fed a standard diet. The primary objective of this study was to determine the effect of inulin on bone health of pigs (using it as an animal model for humans) fed a high-fat diet rich in SFA, having an unbalanced ratio of lysine:metabolisable energy. It was hypothesised that inulin reduces the negative effects of such a diet on bone health. At 50 d of age, twenty-one pigs were randomly allotted to three groups: the control (C) group fed a standard diet, and two experimental (T and TI) groups fed a high-fat diet rich in SFA. Moreover, TI pigs consumed an extra inulin supply (7 % of daily feed intake). After 10 weeks, whole-body bone mineral content (P=0·0054) and bone mineral density (P=0·0322) were higher in pigs of groups TI and C compared with those of group T. Femur bone mineral density was highest in pigs in group C, lower in group TI and lowest in group T (P=0·001). Femurs of pigs in groups TI and C had similar, but higher, maximum strength compared with femurs of pigs in group T (P=0·0082). In conclusion, consumption of a high-fat diet rich in SFA adversely affected bone health, but inulin supplementation in such a diet diminishes this negative effect.

Retensi Nitrogen dan Konversi Pakan Ayam Lokal Persilangan yang Diberi Ekstrak Umbi Dahlia (Dahlia variabilis) sebagai Sumber Inulin

The research was aimed to evaluate used dahlia tubersextract as inulinsource onnitrogen retention and feed conversion of crossbred local chicken (male native chicken × female laying hen). Three weeks old 160 unsex birds with average body weight 180.46±1.21g were kept until 8 weeks. The experimentwas designed ascompletely randomized design with 4 treatments and 4 replications (@10 birds). Treatments applied were T0: basal ration, T1: ration + 0.39% extract, T2: ration + 0.78% extract, T3: ration + 1.17% extract. Parameters observed werenitrogen retention, energy metabolism and feed conversion. The data were statistically analyzed according to ANOVA and continued by Duncan test at the level of 5%. The results showed that feeding dahlia tuberextract significantly affect (P&amp;lt;0.05) nitrogen retentionwith value T0-T3 (1.3g, 1.47g, 1.43g, 1.7g)and feed conversion (4.15, 3.58, 3.75, 3.47), but not significant of energy metabolism. In conclusion, the higher levels of feeding inulin in the form of extract in T3 (1.17%), increase nitrogen retention and improve feed conversionof crossbred local chicken.

Retensi Nitrogen dan Konversi Pakan Ayam Lokal Persilangan yang Diberi Ekstrak Umbi Dahlia (Dahlia variabilis) sebagai Sumber Inulin

The research was aimed to evaluate used dahlia tubersextract as inulinsource onnitrogen retention and feed conversion of crossbred local chicken (male native chicken × female laying hen). Three weeks old 160 unsex birds with average body weight 180.46±1.21g were kept until 8 weeks. The experimentwas designed ascompletely randomized design with 4 treatments and 4 replications (@10 birds). Treatments applied were T0: basal ration, T1: ration + 0.39% extract, T2: ration + 0.78% extract, T3: ration + 1.17% extract. Parameters observed werenitrogen retention, energy metabolism and feed conversion. The data were statistically analyzed according to ANOVA and continued by Duncan test at the level of 5%. The results showed that feeding dahlia tuberextract significantly affect (P&amp;lt;0.05) nitrogen retentionwith value T0-T3 (1.3g, 1.47g, 1.43g, 1.7g)and feed conversion (4.15, 3.58, 3.75, 3.47), but not significant of energy metabolism. In conclusion, the higher levels of feeding inulin in the form of extract in T3 (1.17%), increase nitrogen retention and improve feed conversionof crossbred local chicken.

BLOOD CHOLESTEROL, LDL AND HDL IN CROSSBRED LOCAL CHICKEN FEED INULIN OF DAHLIA TUBERS AS A PREBIOTIC

The feeding inulin of dahlia tubers as a prebiotic related to the quality of the resulting product is a product low cholesterol. The research was aimed to examine the role of inulin as a prebiotic derived from dahlia flower tuber in the form of powder and extract on blood cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) of crossbred local chicken. The research was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each), treatments applied were T0: basal ration, T1: ration + 0.4% powder of dahlia tuber, T2: ration + 0.8% powder of dahlia tuber, T3: ration + 1.2% powder of dahlia tuber, T4: ration + 0.39% extract of dahlia tuber, T5: ration + 0.78% extract of dahlia tuber, T6: ration + 1.17% extract of dahlia tuber. The birds were reared for conditioning from day 1 until 3 week, and dietary treatment was given thereafter until 11 week of age. Parameters observed were blood cholesterol, LDL and HDL. The data were statistically analyzed according to ANOVA and continued to Duncan test at the level of 5% probability. The results showed that feeding inulin in the form of powder or extract significantly (P&lt;0,05) decreased LDL and HDL, but not on blood cholesterol. In conclusion, feeding inulin in the form of powder in 1.2% (T3) and extract in 1.17% (T6), able to reduce levels of LDL and HDL but not yet capable of lowering blood cholesterol of crossbred local chicken.

Effects of dietary oligosaccharide supplementation on growth performance, concentrations of the major odor-causing compounds in excreta, and the cecal microflora of broilers

This study investigated the effects of dietary supplementation with 4 types of oligosaccharides on the growth performance, concentrations of the major odor-causing compounds in excreta and cecal microflora of broilers. Three hundred 21-day-old Archer Abor broilers with an average initial live weight of 702.3 g were randomly divided into 5 dietary treatments: basal diet, basal diet + 5 g/kg of mannan-oligosaccharide (MOS), basal diet + 1.2 g/kg of inulin, basal diet + 1.5 g/kg of fructo-oligosaccharide (FOS), and basal diet +1.25 g/kg of soybean oligosaccharide (SBOS), respectively. Each diet was fed to 6 replicates of 10 birds from d 21 to 42, and body weight and feed intake were recorded. Fresh excreta were sampled from each replicate on d 40, 41, and 42 and stored at −20 °C until further analysis. On d 42, the ceca of killed birds were aseptically removed, and the cecal contents were collected into sterile containers and stored at −80 °C until further analysis. Results showed that feeding inulin, FOS, and SBOS diets resulted in an improvement in daily gain (P < 0.05). Broilers fed the SBOS diet showed lower feed:gain ratio (1.84g:g) than the other groups (P > 0.05). Broilers fed the FOS diet showed the lowest volatile basic nitrogen, pH value, and indole and skatole contents in excreta, and broilers fed the SBOS diet had higher total volatile fatty acids concentrations than control (P < 0.05). The cecal microbial community was measured using the PCR-DGGE, which indicated that the cecal microflora Shannon-wiener index and richness of SBOS-fed broilers were significantly higher than that of the control (P < 0.05). The lowest evenness was recorded in the FOS group, which was significantly lower than the other groups (P < 0.05) except the SBOS group. Based on the sequences of the corresponding 16S rDNA of the DGGE bands, in combination with the contents of the major odor-causing compounds in excreta, it is suggested that uncultured Lachnospiraceae bacterium and Bacteroides sp. were associated with the production of major odor-causing compounds in excreta.

EFEK PENAMBAHAN UMBI BUNGA DAHLIA SEBAGAI SUMBER INULIN TERHADAP KECERNAAN PROTEIN DAN PRODUKTIVITAS AYAM LOKAL PERSILANGAN (Effect of Dahlia Tuber supplementation as Inulin Source on Protein Digestibility and Productivity of Crossbred Local Chicken)

The research was aimed to evaluate feeding effect of powder and extract of dahlia tuber as a sources of inulin prebiotic on protein digestibility and productivity of crossbred local chicken. A total of 280 unsex local crossbred chicken with the age of 22 days and average weight of 180.46±1.21 g was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each). Dietary treatments consist of T0 (basal ration), T1 (T0 + 0.4% dahlia powder), T2 (T0 + 0.8% dahlia powder), T3 (T0 + 1.2% dahlia powder), T4 (T0 + 0.39% dahlia extract), T5 (T0 + 0.78% dahlia extract), T6 (T0 + 1.17% extract dahlia). Treatment was conducted for 8 consecutive weeks. Variables observed were protein digestibility, short chain fatty acid (SCFA), muscle protein mass, muscle calsium mass, and body weight. The data were analyzed using ANOVA and followed by Duncan test at the level of 5%. The results showed that feeding inulin in the form of powder or extract of dahlia tuber significantly (P0.05) increased protein digestibility, SCFA level, and body weight. Treatments T2 until T6 significantly (P0.05) increased muscle calsium mass but did not increase muscle protein mass. In conclusion, the used of 1.2% dahlia powder and 1.17% extract dahlia tuber as an inulin source increase protein digestibility and productivity of crossbred local chicken.Key words: inulin, dahlia tuber, crossbred local chicken

FEEDING EFFECT OF INULIN DERIVED FROM Dahlia variabilis TUBER ON INTESTINAL MICROBES IN STARTER PERIOD OF CROSSBRED NATIVE CHICKENS

The purpose of the research was to study the effects of feeding inulin derived from Dahliavariabilis tuber powder and extract on the existence of intestinal microbes in crossbred native chickenstarter. Experimental animals were 280 unsex crossbred native chickens and powder and extract ofdahlia tuber as source of inulin, which were started to be fed on day 22. The present experiment wasassigned in a completely randomized design with 7 treatments and 4 replications (10 birds each). Thetreatments were: T0 (basal diet/BD), T1 (BD+0.4% powder form), T2 (BD+0.8% powder form), T3(BD+1.2% powder form), T4 (BD+0.39% extract form), T5 (BD+0.78% extract form), and T6(BD+1.17% extract form). Parameters measured were the number of Lactic acid bacteria (LAB),Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage anddaily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan andpolynomial orthogonal test. The results indicated that feeding inulin derived from dahlia tuber powderand extract form significantly (P<0.05) increased LAB population and DBWG, but decreasedEscherichia coli number and intestinal pH, and slow the rate of passage. In conclusion, feeding inulin ofdahlia tuber powder was optimum at 0.9% but that of extract is linier until 1.17%.

Body Resistance and Productive Performances of Crossbred Local Chicken Fed Inulin of Dahlia Tubers

The research was aimed to examine the role of inulin as a prebiotic derived from dahlia flower tuber in the form of powder and extract as a prebiotic on body immunity of crossbred local chicken. The research was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each). The treatments applied were T0: basal ration, T1: ration + 0.4% powder, T2: ration + 0.8% powder, T3: ration + 1.2% powder, T4: ration + 0.39% extract, T5: ration + 0. 78% extract, T6: ration + 1.17% extract. The birds were reared for conditioning from day 1 until day 21, and dietary treatment was given thereafter until 11 wk of age. The data were statistically analyzed according to ANOVA and continued to Duncan test at the level of 5% probability. The results showed that feeding inulin in the form of powder or extract significantly (P

Effect of feeding inulin oligosaccharides on cecum bacteria, egg quality and egg production in laying hens

Effect of feeding inulin oligosaccharides on cecum bacteria, egg quality and egg production in laying hens

Effect of feeding glucose, fructose, and inulin on blood glucose and insulin concentrations in normal ponies and those predisposed to laminitis

Effect of feeding glucose, fructose, and inulin on blood glucose and insulin concentrations in normal ponies and those predisposed to laminitis

Easily fermentable carbohydrates reduce skatole formation in the distal intestine of entire male pigs

Two experiments were conducted to evaluate the effects of feeding different sources of easily fermentable carbohydrates to entire male pigs during the last weeks before slaughter. In Exp. 1, 48 entire male pigs (21.8 and 100.5kg initial and final body weight, respectively) were used to evaluate the effect of adding 0, 3, 6, or 9% chicory inulin (containing 70% inulin) to diets during the last 30days before slaughter on skatole concentration in the hind-gut and adipose tissue and on the composition of microbiota and mucosa morphology in the hind-gut. In Exp. 2, 66 entire male pigs (31.0 and 112.0kg initial and final body weight, respectively) were used to evaluate the effect of adding 20% raw potato starch to diets as a top dressing or adding increasing levels of potato starch (0, 5, 10, 15%, and 20%) to diets prior to pelleting during the last 14days before slaughter on skatole concentrations in hind-gut and adipose tissue and on the composition of microbiota in the hind-gut. Results from Exp. 1 showed a significant reduction in skatole levels in colon and rectum with increasing dietary levels of inulin (linear, P≤0.002). In these diets there was also a reduction of enterobacteria counts in colon descendens (linear, P=0.04) and rectum (linear, P=0.02), and a tendency towards reduced levels of Enterococcus spp. in colon descendens (linear, P=0.09) and rectum (linear, P=0.08). Results from Exp. 2 showed that adding raw potato starch as a top dressing reduced skatole levels in rectum and adipose tissue compared with the control, while adding increasing levels of potato starch to diets prior to pelleting did not affect skatole levels. Adding potato starch as a top dressing or to diets before pelleting did not affect the cultured bacteria in the hind-gut of pigs. Feeding inulin or potato starch did not affect mucosal epithelial cell counts in the cecum or colon in Exp. 1 and Exp. 2, respectively. In conclusion, feeding increasing levels of chicory inulin to entire male pigs during the last period before slaughter reduced the level of skatole in the distal intestine and in adipose tissue, feeding pelleted diets containing up to 20% potato starch had no effect, while feeding 20% raw potato starch as top dressing reduced the level of skatole in the distal intestine and the adipose tissue of the entire male pigs.

Effects of inulin and lactulose on the intestinal morphology of calves

For some time now prebiotics have been proposed to improve health by stimulation of beneficial bacteria in the intestine of humans and animals. The current study is aiming to show effects of feeding of either 2% inulin or 2% lactulose in milk replacer on performance and intestinal morphology of male Holstein–Friesian calves. After 20 weeks of feeding inulin led to significantly higher daily weight gains than lactulose while control animals ranged between the experimental feedings. Ingestion of milk replacer was reduced in lactulose treated animals. Additionally differences of villus height in jejunum (P = 0.07) and ileum (P = 0.03) could be found with an increase for lactulose treated animals and a decrease for inulin treated animals. In ileum the density of proliferative epithelial cells tended to be lower in inulin treated and higher in lactulose treated animals (P = 0.08). Both inulin and lactulose tended to decrease the quantity of goblet cells in the tips of ileal villi (P = 0.07). Both prebiotics can affect performance and intestinal morphology of calves and may as such affect animal health. But effects differ between substances.

Effect of dietary supplementation with inulin and/or benzoic acid on the incidence and severity of post-weaning diarrhoea in weaner pigs after experimental challenge with enterotoxigenic Escherichia coli

The purpose of this study was to evaluate the effects of feeding pigs with inulin and/or benzoic acid on post-weaning diarrhoea (PWD), indices of fermentation in the gastrointestinal tract, and production in pigs experimentally infected with an enterotoxigenic strain of Escherichia coli (ETEC). Forty-eight entire male pigs (Large White×Landrace) aged 21 ± 3 days of age and weighing 4.97 ± 0.08 kg (mean ± SE) were used in a 2×2 factorial experiment, with the respective factors being inulin (0 versus 8%) and benzoic acid (0 vs. 0.5%). Feeding inulin-supplemented diets improved (p = 0.022) the faecal consistency (FC) and reduced (p = 0.001) the incidence of PWD; however, the use of benzoic acid had no effects on PWD or faecal ETEC shedding. Wet faeces (a higher FC score) were associated with increased faecal ETEC shedding (R2 = 0.394, p = 0.001). Inulin reduced the total concentrations of short chain fatty acids (p = 0.029) in the proximal colon. The total concentration of lactic acid was increased by inulin in the caecum (p = 0.007) and proximal colon (p = 0.002). Feeding inulin or benzoic acid had no effects on production after weaning.

Effect of inulin on growth performance, intestinal tract sizes, mineral retention and tibial bone mineralisation in broiler chickens

1. A 5-week feeding trial with 240 one-day-old male broiler chickens was conducted to investigate the responses of performance, intestinal tract measurements, relative apparent retention of minerals (Ca, Mg, Fe, Zn and Cu), and tibial bone measurements and mineral content (ash and Ca) to dietary graded levels of inulin. Treatments consisted of a maize–soybean meal control diet without or with supplemental antibiotic (flavomycin, 40 mg/kg diet) and 4 diets containing inulin at concentrations of 5, 10, 15 and 20 g/kg. 2. Growth performance of birds and morphological measurements of intestinal tract (duodenum, jejunum, ileum and caeca) showed no response to dietary levels of inulin as compared with the control diet and the control diet with flavomycin. 3. Dietary inulin improved the relative apparent retention of Ca, Zn and Cu (up to 18·4, 35·5 and 466%, respectively), did no affect that of Mg and impaired the retention of Fe. Differences among inulin treatments and control with flavomycin were significant only for Mg content. 4. Dietary inulin increased the concentration of ash and Ca in the tibiae. This effect was not reflected in the tibial bone morphology (weight, length and width). In conclusion, the results from the current study suggest that feeding inulin to broiler chickens may have a beneficial effect on bone quality.

Dietary long-chain inulin reduces abdominal fat but has no effect on bone density in growing female rats

New strategies to improve Ca absorption and bone health are needed to address the current state of osteoporosis prevention and management. Inulin-type fructans have shown great promise as a dietary intervention strategy, but have not yet been tested in a young female model. Our objective was to investigate the effect of long chain (LC) inulin on bone mineralization and density in growing, female rats, as well as the quality of growth. Weanling Sprague-Dawley rats were assigned to inulin or cellulose treatments for either 4 or 8 weeks. Growth was measured weekly and quality of growth assessed using fat pad weights and dual-energy X-ray absorptiometry (DXA). Whole body (WB) and selected regions were analysed for bone mineral density (BMD) and body composition by DXA. Serum markers of bone turnover were assessed by enzyme-linked immunosorbent assays. Ca and P concentrations were determined in excised femurs by inductively coupled plasma spectrometry. Feeding inulin resulted in 4 % higher femoral weight (adjusted for body weight) and 6 % less feed intake. Inulin did not affect WB or regional BMD, but was associated with a 28 % lower parametrial fat pad mass, 21 % less WB fat mass and 5 % less WB mass. In summary, LC-inulin lowered body fat mass, without consequence to bone density in growing female rats.