Reduce Feed Costs Research Articles

Implication of dietary barberry (Berberis Vulgaris) leaves inclusion on growth performance, nutrient digestibility, and carcass traits in ostriches

This study determined production performance, nutrient intake, digestibility, and carcass traits from ostrich chicks fed with barberry (Berberis Vulgaris) leaves (BVL) as a replacement for alfalfa hay (AH). For 150 days, 30 male ostrich (Struthio camelus) (9.10 ± 0.89 kg live weight, mean ± SD) were randomly grouped into five dietary BVL inclusion levels to replace AH: a control diet based on 100% AH (CTRL), and four groups with BVL as a substitute for AH at varying levels including 25% (BVL25), 50% (BVL50), 75% (BVL75), and 100% (BVL100). The average daily feed intakes (ADFI) were recorded and birds were weighed monthly. The nutrient intake and digestibility were measured for the following final 5 days of the trial. The ADFI was higher (p = 0.025) for BVL100 and BVL75 than for CTRL. Ostriches fed on BVL50 had the highest average daily gain (ADG, p = 0.025) and the lowest feed conversation ratio (FCR, p = 0.0001). Return per kg BW gain was also enhanced (p = 0.018) with BVL50 feeding. Dietary BVL levels did not affect the digestibility of OM (p = 0.257), CP (p = 0.260), EE (p = 0.610), CF (p = 0.427), and Ash (p = 0.461). Also, there were no changes in AME (p = 0.180) or AMEn (p = 0.670). The hot carcass weight (HCW, p = 0.331) and dressing BW (p = 0.237) were not affected. Overall, the results suggest that BVL can replace AH in ostrich diets without deleterious impact on performance, however, its partial substitution (for 50% AH of diet) has been associated with improved ADG, FCR, and reduced feeding costs.

The Effect of Adding Green and Black Tea Waste Extracts on Rumen Fermentation Parameters by In Vitro Techniques

The increase in global temperatures over the past few decades due to greenhouse gas emissions has raised concerns and necessitated further research in climate change mitigation and adaptation. Methane is a prominent greenhouse gas that significantly contributes to climate change, with a substantial amount generated through fermentation processes occurring in the rumen of ruminant animals. The potential of plant secondary metabolites, especially those derived from tannin-rich plants, warrants investigation to modify rumen fermentation and mitigate methane emissions in livestock diets. The objective of this study was to assess the impact of extracts obtained from green and black tea waste on rumen fermentation dynamics and gas (methane) production, utilizing in vitro methods. For this purpose, rumen fluid was collected from two fistulated sheep and subjected to three treatments: (1) a basal diet (control), (2) a basal diet + green tea waste extract (5% of dry matter), (3) a basal diet + black tea waste extract (5% of dry matter). The study assessed the effects of incorporating extracts from green and black tea waste on various parameters, including digestibility, protozoa population, ammonia nitrogen levels, volatile fatty acids, and methane gas production following a 24-h incubation period. Statistical analysis of the data was conducted using SAS software within a completely randomized design framework. The findings indicated that the addition of green and black tea waste extracts significantly decreased methane gas production (p < 0.05), protozoa count (p < 0.05), and ammonia nitrogen concentrations in rumen fluid (p < 0.05) when compared to the control group. The addition of green and black tea waste extracts has significantly altered the concentration of VFAs in rumen fluid (p < 0.05). Specifically, the addition of green tea waste extract has led to a highly significant reduction in acetic acid, (p < 0.01) and the addition of both extracts has resulted in a significant increase in propionic acid (p < 0.05). Consequently, the results suggest that the inclusion of green and black tea waste extracts in livestock diets may effectively mitigate methane emissions in the rumen, thereby reducing feed costs and reducing environmental pollution.

Determination of the protein value of novel poultry meal ingredients for rainbow trout, Oncorhynchus mykiss

AbstractThe aim of this study was to assess the digestible protein value of three novel poultry products (Pea/Lentil‐PM, Hen on Hen‐PM, and soybean meal (SBM)‐PM), then a growth trial with a 2 × 5 design was conducted where SBM‐PM was included at five levels: 0, 5, 10, 15, and 30% in both a fish meal (FM)‐based diet and a plant protein (PP)‐based diet. Thirty rainbow trout (initial size = 10.7 ± 0.15 g; Troutlodge Inc, Sumner, WA) per tank were stocked into a 15°C recirculating system. Fish in triplicate tanks were fed twice daily to apparent satiation for 12 weeks. SBM‐PM had no effect on final weight (p = 0.3665; FM 233 vs. PP 237) or growth rate (p = 0.4703; FM 2070% vs. PP 2097%). Significant interactions between dietary protein and SBM‐PM for both intake (p = 0.0080) and FCR (p = 0.0081) indicate increased intake of fish fed the plant protein‐based diet without SBM‐PM. Regression modeling of this effect yields a polynomial model with an R‐square of 0.78; p < 0.0001 explained by the relationships: and , respectively. These values suggest that the SBM‐PM can be included up to 30% in rainbow trout feeds to reduce feed costs without negatively impacting performance.

Fibroblast growth factor 1 (FGF1) improves glucose homeostasis, modulates gut microbial composition, and reduces inflammatory responses in rainbow trout (Oncorhynchus mykiss) fed a high-fat diet

High-fat diets (HFDs) are widely used in aquaculture due to their lipid and protein-conserving effects, thereby reducing feed costs. However, prolonged feeding of HFD often leads to metabolic disorders in fish, such as disruption of hepatic lipid homeostasis, liver injury, and disruption of glucose homeostasis. Fibroblast growth factor 1 (FGF1) plays an essential role in controlling glucose levels in the body and dampening immune reactions. However, its impact on teleosts remains poorly researched. The therapeutic potential of recombinant FGF1 (rFGF1) was examined in a 6-week culture experiment involving rainbow trout (Oncorhynchus mykiss) that were fed an HFD. The results revealed that rFGF1 significantly reduced serum glucose levels and hepatic PEPCK and G6PC activities, but improved hepatic glycogen (P < 0.05), compared to the HFD + PBS group. Further experiments indicated that the inhibitory effect of rFGF1 on hepatic gluconeogenesis was mediated by the cAMP signaling pathway and was dependent on the high expression of PDE4D. In addition, rFGF1 increased hepatic glycogen content, which involves the AKT-GSK3β axis. Despite this increase, rFGF1 did not lead to glycogen storage disease, as shown by reduced hepatic inflammation as a result of decreased GOT (glutamic oxaloacetic transaminase), GPT (glutamic pyruvic transaminase), and elevated SOD (superoxide dismutase) in the rFGF1-treated group, accompanied by decreased il-1β, il-6, and xbp-1, and elevated nrf2 and number of hepatocyte autophagosomes. Alterations in gut microbes and short-chain fatty acids (SCFAs) were noted, indicating that rFGF1 caused a notable rise in intestinal Lactobacillus, acetic acid, and butyric acid levels. This study investigated the molecular mechanisms of rFGF1 on glucose metabolism and inflammatory responses in an HFD-fed rainbow trout model, providing new insights to improve the regulation of glucose metabolism in carnivorous fish.

EFFECT OF MAIZE (&lt;i&gt;ZEA MAYS&lt;/i&gt;) COB BASED TOTAL MIXED RATION IN GROWING CALVES

The present study was conducted to study the effect of maize (Zea mays) cob on replacing paddy straw in the Total Mixed Ration (TMR). Twelve cross bred calves of about 5 to 8 months of age with body weight ranging from 41 to 79 kg were divided into two groups of six each in completely randomized design. Five complete diets were prepared (TMR1 to TMR5) using maize cobs at the level of 0%, 25%, 50%, 75% and 100% replacement of paddy straw in the diets containing 12 % CP and 60 % TDN of 50:50 concentrate to roughage ratio. There was significant (P&lt;0.01) differences among the diets in OM, CF, NFE, TA, AIA, NDF, ADF, Lignin, Hemicellulose and Cellulose contents. In vitro rumen fermentation study showed significantly (P&lt;0.01) higher total gas (ml/200 mg/48 h), in vitro dry matter and organic matter degradability in maize cob based ration than paddy straw contained ration (51.17 vs 36.00; 62.87 vs 57.25; 64.80 vs 59.93). Paddy straw (100%) based diet as control ration and maize cob (100 % replacement of paddy straw) based diet as treatment ration fed to growing calves for sixty days in growth trial. A seven day digestion trial was conducted in the middle of the experiment. The digestibility (%) of DM, OM, CP, EE, CF and NFE were significantly (P&lt;0.01) higher in the maize cob based diet fed group than paddy straw based diet fed group. The average body weight gain (kg) and FCR (kg DMI/ kg gain) were significantly (P&lt;0.05) higher in maize cob fed animals (19.47; 6.10) than paddy straw fed animals (16.02; 7.34). The feed cost per unit of weight gain in calves fed paddy straw based diet was numerically higher (30.78 %) than calves fed on maize cob based ration. It could be concluded that maize cob based ration could replace paddy straw at 100 % level in total mixed ration without affecting feed intake and nutrient digestibility and may also improve the body weight gain and reduce feed cost in growing calves.

Feeding of bakery products as replacement of cereal grains alter fecal microbiome and improve feed efficiency in fattening pigs

The re-integration of former foodstuffs, such as bakery products (BP), into pig feed reduces the feed-food competition and increases the sustainability of pig production. Nevertheless, there are concerns about the altered fatty acid profile in BP compared to standard diets and their effects on meat quality and the intestinal microbiome. The present study investigated the effects of replacing 30% cereal grains with BP in the diet on performance, microbiome composition and metabolites in feces of fattening pigs, economic aspects and meat quality characteristics. A total of 132 pigs (Large White × Piétrain; 11 weeks of age) were fed two diets containing 0 or 30% BP until reaching slaughter weight in three replicate batches. Fecal samples were collected for 16S rRNA amplicon sequencing after 6 weeks, whereas body fat samples were collected after 9 weeks on the diet. The results showed that BP can replace cereals in pig diets by up to 30% without impairing animal performance, while improving feed efficiency and reducing feed costs by 9% compared to the control. Additionally, pigs fed the BP diet had a 50%-higher n-3 fatty acid content in the neck and abdominal fat compared to the control. The improved feed efficiency in BP-fed pigs may be related to positive effects on the fecal microbiome, such as a higher alpha diversity and a reduction of fungi and yeasts. Our study provides valuable insights into the benefits of including BP in diets of fattening pigs, which should be investigated in future studies on other BP types.

Crassocephalum crepidioides as a sustainable protein supplement in Chocolatemahseer (Neolissochilus hexagonolepis) diets: Implications for growth,health, and economic viability in sustainable aquaculture

This study investigated the potential of Crassocephalum crepidioides, an abundant weed as a protein supplement in chocolate mahseer (Neolissochilus hexagonolepis) diets. The plant replaced a combination of fish meal and mustard oil cake at 0%, 25%, 50%, 75%, and 100% in experimental diets. A 12-week feeding trial was conducted to assess growth performance, survival, biomass yield, digestive enzyme activity, blood parameters, and economic viability. Results indicated that C. crepidioides can effectively replace up to 61.3% of the combined fish meal and mustard oil cake protein without negatively impacting fish health or growth, while reducing feed costs. Fish fed diets with 25% replacement showed improved weight gain (15.5±0.7 g) and biomass yield (6.05±0.25 kg/m³) compared to the control (14.6±0.8 g and 5.75±0.28 kg/m³, respectively). Survival rates were high across all treatments (94.0- 97.3%). Digestive enzyme activities and blood parameters remained within normal ranges for inclusion levels up to 75%. Economic analysis revealed potential cost savings of up to 15.5% when incorporating C. crepidioides into mahseer diets. This finding presents an opportunity to develop low-cost fish feed for resource-poor farmers in Meghalaya, effectively converting a local weed into a valuable aquaculture input, with broader implications for sustainable aquaculture practices globally.

Performance, Egg Quality, and Composition in Isa Brown Laying Hens Fed with Different Levels of Desmodium tortuosum Leaf Flour.

The objective of this study was to investigate the effects on laying performance, egg quality traits, color, and composition of supplementing a white corn-based diet with Desmodium tortuosum leaf flour. Three hundred 32-week-old hens were distributed to 30 pens of 10 hens each and allocated to six dietary treatments (five replicates per treatment) for 13 weeks. Two control groups of 50 hens received one of either diet Y, based on yellow corn, or diet W, based on white corn. The other groups received a diet based on white corn supplemented with Desmodium tortuosum leaf flour at 2.5%, 5%, 7.5%, or 10% (diet D). Diet D improved laying performance and yolk color at a reduced feed cost per egg but had no effect on cholesterol content. In conclusion, the inclusion of Desmodium tortuosum leaf flour, in a white corn-based diet, is effective in lowering feed cost, increasing egg production, and improving yolk color.

The biological function of Urtica spp. and its application in poultry, fish and livestock.

Urtica species is an angiosperm plant in the Urticaceae family. It serves as a traditional food and medicinal herb, possessing high nutritional value and various bioactive compounds, including polysaccharides, flavonoids, and polyphenolic compounds. In the realm of animal feeds, Urtica spp. can replace traditional protein feed sources and high-quality forage, thereby reducing feed costs. Moreover, Urtica spp. extract exhibits antioxidant and anti-inflammatory properties and boosts immune regulation. Hence, Urtica spp. plays a beneficial role in enhancing animal performance and improving their immune function. Recently, with the development of sustainable farming techniques, the demand for feed additives that prioritize safety, the absence of drug residues, and environmental friendliness have grown. Consequently, Urtica spp. and its extracts have received widespread attention in animal production. This article summarizes the biological functions of Urtica spp. and its application in animal husbandry while also outlining future prospects for its application. It will provide a scientific basis and reference point for the application of Urtica spp. in animal health and breeding.

Aquamimicry feeding system improved growth, physiological status, muscle and intestine histomorphology, and flesh quality of keeled mullet, Liza carinata

The aquamimicry feeding system (AMFS) is one of the modern aquaculture systems that aim to reduce feed costs and water exchange rates to improve the sustainability of aquaculture projects. In AMFS, the aquatic environment is simulated to produce natural food by adding probiotics to carbon sources. Therefore, a 108-day field study was conducted to evaluate the efficiency of AMFS as a solo feed or in combination with a commercial diet for the rearing of keeled mullet, Liza carinata. The L. carinata fingerlings with an initial body weight of 30.0 ± 3.25 g were randomly distributed into three experimental treatments in triplicate rectangular polyethylene ponds (measured 7 × 3 × 1 m) at an initial stocking density of 105 fish per pond. The fish were fed one of the following regimes: the 1st is a commercial floating diet (CFD, 30 % crude protein; T1), the 2nd is a combination of 50 % CFD: 50 % AMFS (T2), the 3rd is a solo of AMFS (T3). Water quality parameters did not differ significantly among different treatments except for a significant reduction in total ammonia nitrogen and a higher zooplankton count in the water of the AMFS group. The fish reared with AMFS as a solo feed (T3) experienced a significant growth performance, somatic indexes, and digestive enzyme activities compared to the fish reared in other treatments. Fish fed AMFS alone (T3) or in combination with CFD (T2) had an improved hematological profile, liver and kidney function indicators, serum lipid, and protein profiles than those fed CFD (T1). Histological and histometric analysis of the intestine showed an improved architecture with AMFS feeding. Furthermore, AMFS feeding increased the diameter of muscle bundles and the protein content of the dorsal muscles. The flesh quality parameters, including water holding capacity, stored loss, drip loss, and frozen leakage rate were improved in fish fed on AMFS alone or in combination with CFD. Therefore, from the above-mentioned beneficial effects of AMFS, it could be used as a promising, economical, and sustainable technology for rearing L. carinata.

Feather meal as a sustainable protein source for aquaculture in Bangladesh: Economic implications

The aquaculture industry is primarily burdened by the costs associated with feed. It is fundamentally dependent on fish meal derived from wild-caught sources as the primary source of protein. With the widespread adoption of alternative feed ingredients for sustainable production, this study evaluated the use of poultry feathers as feed ingredients to produce hydrolyzed feather meal (HFM) and its economic implications in Bangladesh. Samples were collected from the poultry markets of Dhaka city and hydrolyzed using formic acid for hydrolyzed feather meal (HFM) contains a higher percentage of crude protein nutritional analysis. A questionnaire survey was executed to evaluate the market dynamics associated with poultry waste. It was observed that the city generates approximately 1000 metric tons of poultry waste every day. Among them, 95% of the waste is thrown away. It was discerned that 0.5 metric tons of feather meal could be generated using one ton of raw feathers. Annually, 3000 metric tons of feather meal could be produced with a value of 0.9 million US$. The HFM contains a higher percentage of crude protein (86.53±0.02%) with crude fat (5.03±0.03%). It contains most of the important amino acids that are beneficial for the growth of fish. The price of the HFM was estimated at 0.27 to 0.30 US$ per kilogram, notably lower compared to fish meal (1.1 US$), and bone meal (0.7 US$). The physical properties of the HFM were found satisfactory. Thus, domestically sourced hydrolyzed feather meal could be used as a feed ingredients for aquaculture operations to reduce feed costs. HFM has the potential to be utilized as a cost-effective feed ingredient, thus lowering overall feed expenses, and presenting a substantial commercial potential.

Additive effects of Bridelia ferruginea leaf meal as a partial replacement for soybean meal on nutrients digestibility, growth performance, and carcass characteristics of weaner rabbits

BackgroundProtein is a crucial nutrient for enhancing livestock performance and productivity, traditionally sourced from soybean and fish meal. However, issues like erratic availability and rising prices necessitate exploring alternative protein sources. Tropical plants, especially leaf meals, are being considered to replace or supplement conventional protein sources in rabbit diets. Bridelia ferruginea, a plant abundant in West Africa, is recognized for its medicinal properties, but its potential as an animal feed is largely unexplored. This study aims to evaluate the impact of graded levels of Bridelia ferruginea leaf meal (BFLM) as a partial substitute for soybean meal in the diets of weaner rabbits.Materials and methodsThe experiment was conducted at the Animal Science Department of Kwame Nkrumah University of Science and Technology in Ghana. BFLM was obtained by air-drying harvested leaves and milling them. Four diets were formulated, with BFLM partially replacing soybean meal at levels of 0%, 10%, 20%, and 30%. Representative samples of BFLM and all formulated diets were analyzed for their proximate composition and detergent fibre fractions. Twenty-four weaner rabbits were assigned to these diets in a Completely Randomized Design. The study covered a 10-week period, assessing growth performance, nutrient digestibility, carcass characteristics, and economics of production.ResultsThe chemical composition of BFLM revealed favorable attributes for rabbit feeding, with 20.5% crude protein and 92.6% dry matter. Growth performance declined at 30% BFLM inclusion, indicating a possible impact of anti-nutritional factors. Feed conversion ratio increased at higher BFLM levels. Nutrient digestibility showed a decline in dry matter digestibility at 30% BFLM. Carcass characteristics were affected at higher BFLM levels, with dressing percentage decreasing. Weights of internal organs were generally not negatively affected by the inclusion of BFLM. Economic analysis indicated a linear reduction in feed cost with increasing BFLM inclusion.ConclusionThe study suggests that BFLM can be incorporated up to 20% in weaner rabbit diets without compromising productivity, nutrient digestibility, or dressing percentage. Beyond 20%, adverse effects on growth performance and some carcass characteristics were observed. Economically, BFLM incorporation up to 20% lowered feed costs without compromising feed efficiency. Incorporation of BFLM up to 20% in weaner rabbit diets had no adverse effects on the weights and functions of the heart, liver, and kidneys. Overall, BFLM presents a promising alternative protein source for weaner rabbit diets, contributing to cost-effective and sustainable livestock production.

Evaluation of Growth Performance and Economic Efficiency of Immature Lohmann Brown Layers Fed Graded Levels of Undeshelled Defatted Moringa oleifera Seed Cake

In a 22-weeks experiment, the impact and economic efficiency of feeding undeshelled defatted Moringa oleifera seed cake (UDMOSC) to Lohmann Brown layer chicks and pullets were investigated. The study had two phases, focusing on chicks (0-8 weeks) and pullets (9-22 weeks). A total of 150-day-old chicks were randomly assigned to five dietary treatments (0%, 5%, 10%, 15% and 15% with Burgazyme inclusion), each replicated three times, and each replicate containing ten (10) birds in a completely randomised design. Data collected was subjected to analysis of variance (ANOVA) using the GenStat statistical package (2008). In the initial phase, chicks fed dietary UDMOSC consumed less feed but had similar (p ˃ 0.05) final body weight compared to the control group. Chicks fed the control, and 5% UDMOSC gained more weight with better feed conversion. Chicks fed 10% UDMOSC had higher mortality. In the second phase, pullets fed the control diet and 15% UDMOSC with the enzyme (UDMOSC15E) showed significantly higher (p = 0.01) feed intake. Final body weight and body weight gain were notably higher (p = 0.001) in pullets fed the control diet. Feed conversion ratio and mortality rate did not significantly differ (P ˃ 0.05) among treatments. Dietary UDMOSC reduced feed cost per kilogram of body weight gain for starters and pullets. While dietary UDMOSC did not significantly affect starter growth, it lowered total feed cost per bird for starters and pullets except for UDMOSC15E, accruing 20.54 – 27.81%. and 25.39 – 40.19% percentage profit range, respectively. Dietary UDMOSC depressed the growth of starters and pullets, but it was economically advantageous by reducing the cost per kilogram of body weight gain for both groups. The negative impact of dietary UDMOSC on the growth of starters and pullets suggests that including UDMOSC in the immature layer diets should be cautiously approached for economic gain and, therefore, recommended for the pullet stage at an inclusion level not exceeding 10%.

The effect of blood meal to induce aquaculture performance of striped catfish (Pangasionodon hypophthalmus Sauvage, 1878)

Abstract The high price of fish meal as feed is the main problem in cultivating striped catfish. The use of blood meal, waste from slaughterhouses, can be an alternative to improve aquaculture performance while reducing feed costs. This research aimed to determine the effect of using blood meal as a source of feed protein on the growth performance of striped catfish. The research design used was a Completely Randomized Design (CRD) consisting of five treatments and three replications. The treatments used were A (blood meal 0%, fish meal 100%) as control, B (blood meal 25%, fish meal 75%), C (blood meal 50%, fish meal 50%), D (blood meal 75%, fish meal 25%), and E (blood meal 100%, fish meal 0%). The results showed that the average body weight and specific growth in treatments B (8.25 ± 0.63b g, 3.51 ± 0.13b %/day) and C (8.26 ± 0.73b g, 3.52 ± 0.14b %/day) had the same performance as the control. It is concluded that blood meal can be substituted and combined with fish flour with a maximum composition of 50% to improve the performance of striped catfish aquaculture. Further research to increase the nutrition of blood meal must be conducted to improve the aquaculture performance of striped catfish.

Appropriate Technological Innovation Towards An Independent Village Based On Agrotourism In Sumbergedang Village

Sumbergedang Village is one of the villages located in Pandaan District, Pasuruan Regency, East Java Province, which has green and cool natural conditions because it is located at the foot of Mount Penanggungan, West Side and has views of Mount Arjuno. Potential natural resources include rivers with a calm flow from springs throughout the year, livestock farming with sheep, goats and fish and around 30 springs. The aim of the Assisted Village Program activities is to exploit this natural potential into agrotourism so that it becomes an independent and prosperous village. The activity method involves direct assistance by the entire program implementation team and 5 students who work in the field for one semester. The activity begins with preparation by determining the schedule &amp; compiling measurement instruments to carry out pre- and post-activity evaluations. Training includes: Building an entrepreneurial spirit, business motivation, digital bookkeeping and marketing, training on the operation of coper and feed ingredient flouring machines, making feed formulas and mixing feed, training on making various chips and drinks, training on the use of chopping machines for packaging chips and packaging drinks as well as Institutional development is directed at fostering group management. The results of the activity include: all partners consisting of livestock and fish farmer groups, village PKK, BUMDES and MSMEs increased their entrepreneurial spirit after receiving business motivation training. The complete result of this Assisted Village activity is that the use of appropriate technology (TTG) in the form of a multipurpose machine is able to increase the weight gain of sheep by more than 150 grams/head/day so that fattening time is shorter. With a fish feed machine, groups can make their own feed, thereby reducing feed costs to Rp. 10,000/ kg feed. The use of a chopping machine can increase the production capacity of chips to 150/200 kg/day and the use of a beverage packaging machine can increase the capacity of sinom drinks to 25 liters/day. The conclusion of this activity is that the application of appropriate technology is really needed by society to increase people's income and welfare.

Effects of high-level dietary distillers dried grains with solubles supplemented with multienzymes on growth performance, nutrient utilization, intestinal morphology, and pellet quality in broiler chickens.

With the increasing cost of bulk raw materials and advancements in the feed enzyme industry, corn distillers dried grains with solubles (DDGS) have shown more opportunities for use in broiler diets. Supplementation with multiple enzymes could mitigate anti-nutritional factors in DDGS, enhance nutrient digestibility, and thereby increase its utilization in broiler diets, leading to reduced feed costs. This study evaluated the effects of multienzyme supplementation on growth performance, nutrient utilization, intestinal morphology, and pellet quality in broiler chickens fed diets containing conventional levels of DDGS (C-DDGS) and higher levels of DDGS (H-DDGS). A total of 800 1-day-old Cobb 500 chicks was assigned to four dietary treatments with eight replicates of 25 birds each: C-DDGS (5% DDGS in Starter and 10% in Grower), C-DDGS + Enzyme (C-DDGS diet supplemented with multienzyme), H-DDGS (10% and 20%) + Enzyme (H-DDGS diet supplemented with multienzyme, 10% DDGS in Starter and 20% in Grower), and H-DDGS (15% and 30%) + Enzyme. The C-DDGS + enzyme diet increased (p < 0.05) body weight gain (BWG), reduced the feed conversion ratio, enhanced (p < 0.05) digestibility of dry matter (DM), crude protein, and hemicellulose (HC), and improved (p < 0.05) intestinal villus height and villus: crypt ratio of broilers. The H-DDGS (10% and 20%) + enzyme diet exhibited no difference in (p > 0.05) growth performance, nutrient digestibility (except HC), and intestinal morphological parameters, whereas the H-DDGS (15% and 30%) + enzyme diet decreased (p < 0.05) feed intake and BWG and reduced (p < 0.05) energy and DM digestibility by impact (p < 0.05) intestinal morphology compared with the C-DDGS enzyme-free diet. The H-DDGS diet had lower (p < 0.05) pellet hardness and poorer durability than the C-DDGS diet. Supplementing multienzyme in the C-DDGS (5% and 10%) diet improved growth performance from day 0 to 28 and diminished growth performance in the H-DDGS (15% and 30%) diet by influencing intestinal morphology and feed pellet quality in broiler chickens. In addition, when supplemented with multienzyme, the dietary DDGS level can be safely included at levels of 10% in 0-7 days and 20% in 8-28 days of age.

Dietary fiber source and direct-fed microbial supplementation effects on lactation performance and feeding behavior of high-producing dairy cows

The objective of this study was to evaluate the effects of dietary fiber source and direct-fed microbial supplementation on lactation performance and feeding behavior of high-producing dairy cows. Sixty-four multiparous Holstein cows (3.5 ± 1.6 lactations; 76 ± 22 DIM and 735 ± 67 kg of BW at covariate period initiation) and 32 gate feeders were enrolled in a study with a completely randomized design and a 2 × 2 factorial arrangement. Cows and gate feeders were randomly assigned to treatments (16 cows and 8 gate feeds per treatment). Cows were allowed 1 week to acclimate to feeding gates followed by a 2-week covariate period. During the acclimation and covariate periods, all cows were fed the same diet to meet or exceed the nutrient requirements. Following the covariate period, cows were enrolled in a 8-week treatment period during which cows were randomly assigned to 1 of 4 treatments consisting of forage inclusion in the diet, either 45.8% (LF) or 56.7% (HF) of DM, and the supplementation of 75 mL/hd/d of a direct-fed microbial (DFM) containing herbal extracts (mallow, mint, and sage), L. plantarum, L. buchneri, Saccharomyces cerevisiae, and sugar cane molasses (Valibiom Mix, Valibiotics, Traiskirchen, Austria) or without supplementation (CON). The average covariate period value of each variable was used as a covariate. Three-way interactions were observed for DMI and feed efficiency. Dry matter intake was 2 to 3 kg greater for LF-DFM than HF-CON and HF-DFM during wk 2, 3, 5, and 8 of the treatment period. Milk production was 2.1 kg/d greater for LF than HF diets. Both milk fat and MUN concentrations were greater for HF than LF diets. Conversely, milk protein concentration was lower for HF than LF diets. The respiration rate measured in the morning was lower with DFM supplementation than CON. Rectal temperature measured in the morning and averaged for the day were greater for LF than HF diets. Under the conditions of the present study, feeding high-forage diets may be an alternative for producers to reduce feeding costs depending on the price of purchased feeds. However, non-forage fiber sources (i.e., soy hulls) must be considered when producers are challenged by either forage shortages or forage with a lower nutritive value. Additionally, DFM supplementation reduced respiration rate in the morning and affected meal behavior of lactating cows.

Harnessing the role of nutrigenomics for feed efficiency utilization in cattle: Opportunities and challenges

Nutrigenomics, the study of the interaction between nutrition and genes, presents a transformative approach to enhancing feed efficiency in cattle. This field leverages genetic insights to optimize dietary formulations, aiming to improve nutrient utilization and overall productivity. With the global demand for meat and dairy products rising, increasing feed efficiency is critical for economic viability and environmental sustainability in the livestock industry. The role of nutrigenomics in cattle feed efficiency encompasses several key areas. Precision nutrition is one of the foremost opportunities, enabling the design of diets tailored to the genetic profiles of individual animals. This personalized approach can significantly enhance nutrient absorption and reduce feed costs. Additionally, nutrigenomics can inform selective breeding programs, identifying genetic markers associated with superior feed efficiency traits. By breeding cattle with these desirable traits, farmers can achieve herds that require less feed to produce the same amount of meat or milk, thereby improving profitability and reducing environmental impact. The gestation period in cattle, typically around 283 days, is critical for breeding management. Proper timing ensures optimal calving intervals and productivity. Monitoring gestation aids in anticipating calving dates, ensuring adequate nutrition, health care, and minimizing complications, thereby enhancing overall herd fertility and efficiency. However, the implementation of nutrigenomics faces several challenges. Practical challenges include integrating genetic data into everyday farming practices and ensuring farmers are adequately trained. Ethical and regulatory concerns also arise, particularly regarding genetic modifications and the acceptance of such technologies by consumers and regulators. Moreover, initial investments in nutrigenomic technologies and potential market barriers may hinder widespread adoption. Current research and case studies demonstrate the potential of nutrigenomics to revolutionize cattle feed efficiency. Successful applications in various regions highlight both the feasibility and benefits of this approach. Future advancements in genomic technologies, coupled with collaborative efforts between industry and academia, are expected to overcome existing challenges. Policy support and funding will be crucial in facilitating this transition. Harnessing nutrigenomics for feed efficiency in cattle offers promising opportunities but requires addressing significant challenges. The integration of genetic insights into nutrition and breeding practices stands to enhance livestock productivity, economic gains, and environmental sustainability, paving the way for a more efficient and sustainable livestock industry. Keywords: Nutrigenomics, Feed efficiency, Genetic markers, Cattles,

Fast and reliable method for analysis of derivatized plasma amino acids by liquid chromatography-single quadrupole-mass spectrometry

The pool of free, genetically encoded AA in plasma plays an essential role not only as substrate for every protein synthesized in the body but also as signaling molecules that regulate a wide range of physiological processes. Here we present a method for the analysis of 19 of the 20 encoded AA (except Cys) in dairy cow plasma. Isolated plasma or standards for the 19 AA were gravimetrically mixed with an internal standard mix consisting of 13C isotopes of each AA. Plasma proteins were precipitated on acetonitrile and supernatants transferred to glass vials. For pre-column derivatization, plasma supernatants were buffered with sodium borate (pH 9.5 −10), and AA were derivatized with 9-fluorenylmethoxycarbonyl (Fmoc) chloride. Analytes were isolated by solid-phase extraction using a strong-anionic ion exchange column and dry eluates were reconstituted in mobile phase consisting of 70% water solution of ammonium formate and 30% acetonitrile. Amino acid derivatives were separated by reverse phase liquid chromatography over 17.5 min with a C18 column in which acetonitrile increased to 80% over the first 11 min of the method, before returning to initial levels. Electrospray ionization on negative mode was used for most AA, except Arg and Pro, for which positive mode yielded superior results. Single or double (only Lys) derivatives were measured by single quadrupole mass spectrometry. We hypothesized that pre-column Fmoc derivatization would yield optimal resolution for quantitative analysis of the 19 targeted AA and their respective 13C internal standards, with limits of quantitation beyond physiological ranges. All the 19 AA were detected with minimal background noise. An 11-point standard curve was developed for each AA. Limits of quantitation were beyond concentrations observed in plasma samples of lactating dairy cows, except for Gly that upper curve points had to be removed to maintain linearity, limiting quantitation to the upper range of physiological concentration. After removing the 4 highest concentrations from the Gly standard curve, coefficients of determination were greater than 0.999 for all the AA. Recovery of spiked AA from plasma samples ranged from 89.9% for Phe to 100.3% for Trp. Instrument repeatability averaged 0.91 and ranged from 0.33 for Val to 2.29 for Arg. Meanwhile, sample preparation method repeatability averaged 2.02 and ranged from 1.14 for Tyr to 3.34 for Arg. While robust methods have been developed, they depend on either availability of sophisticated instruments, mostly limited to core facilities (i.e., tandem MS methods), long and expensive chromatography without specific internal standards for each AA (i.e., HPLC-UV and HPLC-FLD), or unstable derivatization (GC-MS). Here we describe a method with high throughput, stable derivatization, high precision and recovery, and potentially more affordable than most existing methods. This method could help dairy nutritionists to consider plasma AA information for diet formulation strategies, potentially reducing feeding costs and N emissions.

Vitamin 25(OH)D3, E, and C Supplementation Impact the Inflammatory and Antioxidant Responses in Piglets Fed a Deoxynivalenol-Contaminated Diet and Challenged with Lipopolysaccharides.

Using alternative ingredients or low-quality grain grades to reduce feeding costs for pig diets can introduce mycotoxins such as deoxynivalenol (DON) into feed, which is known to induce anorexia, inflammation, and oxidative stress. Adding vitamin 25(OH)D3 or vitamins E and C to the feed could increase piglets' immune system to alleviate the effects of DON. This study used 54 pigs (7.8 ± 0.14 kg) in 27 pens (2 pigs/pen) with a vitamin 25(OH)D3 or vitamin E-C supplementation, or their combination, in DON-contaminated (5.1 mg/kg) feed ingredients over 21 days followed by a lipopolysaccharide (LPS) challenge (20 µg/kg BW) 3 h prior to euthanasia for 1 piglet per pen. DON contamination induced anorexia, which reduced piglet growth. DON also induced immunomodulation, oxidative stress, and downregulated vitamin D status. The vitamin E and C supplementation and the combination of vitamins E, C, and 25(OH)D3 provided protection against DON contamination by not only decreasing blood and liver oxidative stress markers, but also by increasing antioxidant enzymes and tocopherol levels in blood, indicating improved antioxidant defense mechanisms. The combination of vitamins also restored the vitamin D status. After LPS challenge, DON contamination decreased intestinal and liver antioxidant statuses and increased inflammation markers. The addition of vitamins E and C to DON-contaminated feed reduced markers of inflammation and improved the antioxidant status after the LPS immune stimulation. The combination of all these vitamins also reduced the oxidative stress markers and the inflammation in the intestine and mesenteric lymph nodes, suggesting an anti-inflammatory effect.