Ad Libitum Feeding Research Articles

Different time-restricted feeding patterns potentially modulate metabolic health by altering tryptophan metabolism of gut microbes in pigs

Time-restricted feeding has emerged as a preferred approach for alleviating metabolic disorders, but the potential microbiological mechanism remains poorly understood. This study used a growing pig model to mimic common-sense eating habits. Four feeding patterns were set up, including ad libitum feeding (ALF) for daily irregulated eating habits, time-restricted feeding (TRF) for three meals a day, early time-restricted feeding (eTRF) for skipping dinner and mid-day time-restricted feeding (mTRF) for skipping breakfast. The results showed that the three time-restricted feeding patterns (TRF, eTRF and mTRF) resulted in a reduction of hepatic fat accumulation and a decrease in hepatic function markers compared to the ALF pattern. However, this was independent of food consumption. Transcriptome analysis of the liver showed that the three time-restricted feeding patterns downregulated the expression of genes related to gluconeogenesis, β-oxidation, lipid accumulation, and urea cycle, and upregulated the expression of genes related to lipogenesis and glycolysis compared to the ALF pattern. Microbiome and metabolome analyses showed that Lactobacillus enriched in the colon of pigs in three time-restricted groups were negatively correlated with serum triglyceride. Meanwhile, three time-restricted feeding patterns elevated the levels of the microbial metabolite indole-3-lactic acid, which was further confirmed to reduce excessive hepatic lipid accumulation in vitro. Overall, time-restricted feeding potentially improved metabolic health by modulating gut microbiota and metabolites.

PSVII-30 Winter-feeding high concentrate diets reduces enteric methane emissions pre-calving in beef cows

Abstract Objectives were to evaluate the effects of winter-feeding forage (2.02 Mcal/kg dietary ME) vs. concentrate (2.84 Mcal/kg of dietary ME) diets pre- and post- calving on enteric methane emissions, dry matter intake (DMI), and performance of beef cows. Forty-six pregnant (210 ± 10 d of gestation) Angus and Simmental-Angus cows [n = 46; body weight (BW) = 630 ± 12.0 kg) were blocked by breed, age, and BW and assigned to one of two treatments: 1) Ad-libitum feeding of forage-based diet (HFOR; n = 23); or 2) a concentrate corn-based diet with a restricted intake of 1.2% BW (HCON; n = 23). Cows were housed in a group-pen at the South Dakota State University Cow-Calf Education and Research Facility (Brookings, SD). Feed consumption was tracked using an electronic feeder (Insentec, The Hague, Netherlands) and enteric methane emissions were monitored using the GreenFeed trailer system (C-Lock Inc., Rapid City, SD). Body weight measurements were taken bi-weekly. Treatments started being applied on d 50 (± 10) pre-calving and will continue to be applied up to d 84 post-calving. Preliminary data reported herein was collected from d 42 pre-calving up to d 42 post-calving. Performance and dry matter intake data were analyzed as repeated measures using the MIXED procedure of SAS with treatment, period, and their interaction as fixed effects and animal as a random effect. Enteric emissions were analyzed using a linear mixed effects model with animal as a random effect in R, with a post-hoc contrast among treatments. Pre-calving, HFOR had greater (P < 0.01) CH4 emissions than HCON (264 ± 9.19 g/d and 246 ± 9 g/d for HFOR and HCON, respectively). Both groups presented dynamic changes in CH4 emissions over time (P < 0.01). Post-calving, no differences were observed between treatments (P = 0.55); however, temporal changes (P < 0.01) and treatment × d interaction (P < 0.01) persisted, indicating ongoing diet-temporal interplay. No treatment × period interactions (P ≥ 0.16) were observed for DMI and performance. As designed, dry matter intake was less (P < 0.01) for HCON (8.66 ± 0.45 kg) than HFOR (17.3 ± 0.45 kg) cows. Further, dry matter intake was less (P < 0.01) pre-calving (11.11 ± 0.28 kg) than post-calving (14.85 ± 0.48 kg). Cows in the HFOR group were heavier (P = 0.01) than HCON (646.22 ± 14 kg vs. 593.43 ± 14 kg, respectively) likely due to ruminal fill; and as expected cows were heavier (P < 0.01) pre- than post-calving, with the greatest BW observed on d 14 pre-calving (639.52 ± 9.92 kg). These data indicate that high concentrate corn-based diets may be used in dry-lot settings to optimize nutritional management and reduce methane emissions when compared with conventional high forage winter-feeding strategies.

Effect of Gender on Metabolic and Neurodevelopmental Parameters of Postnatally Growth-Restricted Rats Undergoing Catch-Up Growth.

To determine the long-term effects of early postnatal malnutrition and various degrees of catch-up growth on metabolic (serum glucose, leptin, triacylglycerides) and neurodevelopmental parameters (learning and memory) among male and female rodent models, mimicking human preterm infants. Randomized controlled trial. Place and Duration of the Study: CMH Multan Insitute of Medical Sciences, from September 2021 to December 2021. This study included 142 neonatal Wister rats, stratified into subgroups to mimic the human preterm infant model of postnatal malnutrition and catch-up growth. Metabolic consequences were assessed via serum analysis of glucose, leptin, and triacylglycerides. The neurocognitive comparison was made among subgroups via a passive avoidance test. Gender-specific comparison of all quantitative parameters was made among subgroups. Malnourished rats with accelerated catch-up growth achieved similar weight gain as normally fed rats when provided with adlibitum feeding in both males (p = 0.92) and females (p >0.99). Rats undergoing accelerated catch-up growth exhibited higher fasting serum glucose levels compared to those undergoing no, or normal catch-up growth (p <0.001). Malnourished female rats undergoing accelerated (p = 0.007), or no catchup growth (p = 0.004) exhibited significant deficits in learning and memory as compared to normally fed rats. Female malnourished rats with normal catchup growth exhibited no neurocognitive deficit as compared to normally fed rats (p = 0.08). Accelerated catch-up growth effectively addresses somatic growth disparities, while normal catch-up growth offers more favourable metabolic and neurodevelopmental outcomes. Particularly, female malnourished rats exhibited poor neurodevelopment in response to both accelerated and no catch-up growth. Gender-specific variations in neurodevelopment underscore the need for personalised care approaches for preterm nutritional care. Growth retardation, Leptin, Extrauterine growth restriction, Malnutrition, Neurodevelopment.

Lateral parabrachial nucleus astrocytes control food intake.

Food intake behavior is under the tight control of the central nervous system. Most studies to date focus on the contribution of neurons to this behavior. However, although previously overlooked, astrocytes have recently been implicated to play a key role in feeding control. Most of the recent literature has focused on astrocytic contribution in the hypothalamus or the dorsal vagal complex. The contribution of astrocytes located in the lateral parabrachial nucleus (lPBN) to feeding behavior control remains poorly understood. Thus, here, we first investigated whether activation of lPBN astrocytes affects feeding behavior in male and female rats using chemogenetic activation. Astrocytic activation in the lPBN led to profound anorexia in both sexes, under both ad-libitum feeding schedule and after a fasting challenge. Astrocytes have a key contribution to glutamate homeostasis and can themselves release glutamate. Moreover, lPBN glutamate signaling is a key contributor to potent anorexia, which can be induced by lPBN activation. Thus, here, we determined whether glutamate signaling is necessary for lPBN astrocyte activation-induced anorexia, and found that pharmacological N-methyl D-aspartate (NMDA) receptor blockade attenuated the food intake reduction resulting from lPBN astrocyte activation. Since astrocytes have been shown to contribute to feeding control by modulating the feeding effect of peripheral feeding signals, we further investigated whether lPBN astrocyte activation is capable of modulating the anorexic effect of the gut/brain hormone, glucagon like peptide -1, as well as the orexigenic effect of the stomach hormone - ghrelin, and found that the feeding effect of both signals is modulated by lPBN astrocytic activation. Lastly, we found that lPBN astrocyte activation-induced anorexia is affected by a diet-induced obesity challenge, in a sex-divergent manner. Collectively, current findings uncover a novel role for lPBN astrocytes in feeding behavior control.

287 The effect of cooling pad and Moringa oleifera on lactating sows under heat stress

Abstract Lactating sows and their litters are particularly vulnerable to heat stress (HS). HS decreases sow fertility, feed intake, milk production, and litter growth. Approaches are needed to mitigate the negative effects of HS on animal welfare and reduce oxidative damage to tissues associated with HS. Recently, electronic cooling pad (ECP) were designed to remove heat through conductive cooling. The objective of this study was to test the efficacy of ECP and feeding an antioxidant, Moringa oleifera (4% of the diet, M), to sows from late gestation to weaning on their welfare and production performance. A study was conducted using a 2 by 2 factorial arrangements of treatments with mixed parity Yorkshire x Landrace sows (n = 48) mated to Duroc sires. The sows were exposed to moderate HS conditions from gestation d 100 to d 21 of lactation (weaning). To achieve moderate HS analogous to a summer day, farrowing room temperature was increased incrementally from 800 to 1100 h to 32º C and slowly decreased to 26 ºC from 1700 to 2000 h. Sows were limit-fed their specific diets during gestation (1.36 kg 2X/d) and fed for ad-libitum feed intake post farrowing. Treatments included HS+control corn-soy meal diet (CS), HS+M, ECP+CS, ECP+M (n = 12/treatment). Sow RR and RT were measured four times daily (0600, 1300, 1600, 1900 h) and sow feed intake during lactation was measured along with body weight (BW) change from farrowing to weaning. Birth litter size was determined, and growth of standardized litters (n = 13 piglets/sow) was measured. Milk was collected during active farrowing (d 0) and on d 3 and d 14 post-farrowing and analyzed for macronutrient content. The use of ECP decreased lactating sow RR (Lsmeans HS = 66.5 bpm, ECP = 28.2 bpm, P ≤ 0.0001), RT (Lsmeans HS = 39.2 ºC, ECP = 38.8 ºC, P = 0.003), and lactation BW loss (Lsmeans HS = -21.67, ECP = -5.1 kg P = 0.002). ECP by time-of-day interactions (P &amp;lt; 0.01) existed for RR and RT with ECP having a greater impact from 1300 to 1900 h than at 0600 h. ECP increased lactation feed intake (P &amp;lt; 0.02). An ECP by diet interaction (P = 0.0004) indicated ECP increased lactation feed intake for the sows fed the CS diet (6.48 kg/d vs 4.42 kg/d, P = 0.0004) but did not affect feed intake of sows fed the M diet. Treatment did not affect litter weaning weight (P = 0.14). M increased percent fat in milk (Lsmeans M = 12.28, CS = 11.06, P = 0.05) across all days of lactation. The reduction in sow RR and RT combined with increased feed intake and decreased loss of BW during lactation indicate that ECP improved sow wellbeing in the HS environment. Neither cooling nor M affected litter growth, despite increased fat content of milk of M supplemented sows and increased feed intake of ECP-CS sows.

Impact of sustained calorie restriction and weight cycling on body composition in high-fat diet-fed male and female C57BL/6J mice.

The objective of this study was to investigate body composition changes with weight cycling (WC) among adult C57BL/6J mice with diet-induced obesity. A total of 555 single-housed mice were fed a high-fat diet ad libitum (AL) from 8 to 43 weeks of age. The 200 heaviest mice of each sex were randomized to the following four groups: ever obese (EO, continued AL feeding); obese weight loser (OWL, calorie-restricted); obese weight loser moderate (OWLM, body weight halfway between EO and OWL); and WC (diet restricted to OWL followed by AL refeeding cycles). Body weight and composition data were collected. Linear regression was used to calculate residuals between predicted and observed fat mass. Linear mixed models were used to compare diet groups. Although weight loss and regain resulted in changes in body weight and composition, fat mass, body weight, and relative body fat were not significantly greater for the WC group compared with the EO group. During long-term calorie restriction, males (but not females) in the OWLM group remained relatively fatter than the EO group. WC did not increase body weight or relative fat mass for middle-aged, high-fat diet-fed adult mice. However, long-term moderate calorie restriction resulted in lower body weight but greater "relative" fat in male mice.

Transcriptome Analysis of Compensatory Growth and Meat Quality Alteration after Varied Restricted Feeding Conditions in Beef Cattle.

Compensatory growth (CG) is a physiological response that accelerates growth following a period of nutrient limitation, with the potential to improve growth efficiency and meat quality in cattle. However, the underlying molecular mechanisms remain poorly understood. In this study, 60 Huaxi cattle were divided into one ad libitum feeding (ALF) group and two restricted feeding groups (75% restricted, RF75; 50% restricted, RF50) undergoing a short-term restriction period followed by evaluation of CG. Detailed comparisons of growth performance during the experimental period, as well as carcass and meat quality traits, were conducted, complemented by a comprehensive transcriptome analysis of the longissimus dorsi muscle using differential expression analysis, gene set enrichment analysis (GSEA), gene set variation analysis (GSVA), and weighted correlation network analysis (WGCNA). The results showed that irrespective of the restriction degree, the restricted animals exhibited CG, achieving final body weights comparable to the ALF group. Compensating animals showed differences in meat quality traits, such as pH, cooking loss, and fat content, compared to the ALF group. Transcriptomic analysis revealed 57 genes and 31 pathways differentially regulated during CG, covering immune response, acid-lipid metabolism, and protein synthesis. Notably, complement-coagulation-fibrinolytic system synergy was identified as potentially responsible for meat quality optimization in RF75. This study provides novel and valuable genetic insights into the regulatory mechanisms of CG in beef cattle.

Investigation of Heavy Metal Levels in Blood of Anatolian Water Buffalo (Bubalus bubalis) Raised in Bitlis Province

In this study, which aims to determine the levels of heavy metals, in the blood of 20 Anatolian buffaloes (Bubalus bubalis) aged 12-18 months, raised in Bitlis province, Güroymak District and fed with ready-made feed ad-libitum in pasture + pen conditions. 3 macro minerals and 14 micro minerals were found in the blood. The levels of a total of 17 minerals were measured by inductively coupled plasma optical emission spectrometry (ICP-OES). Among the macro minerals levels were Ca 43.896±1.216 ppm, Mg 11.381±0.325 ppm, K 133.175±13.959 ppm and micro minerals levels were Al 0.710±0.090 ppm, As 0.027±0.006 ppm, Ba 0.038±0.006 ppm, Co 0.006±0.002 ppm, Cr 0.055±0.010 ppm, Cu 0.218±0.033 ppm, Fe 0.83 2±0.051 ppm, Mn 0.024±0.003 ppm, Mo 0.009±0.002 ppm, Se 0.084±0.009 ppm, Sr 0.050±0.004 ppm, Ti 0.046±0.011 ppm, V 0.058 ±0.014 ppm and Zn 0.168±0.025 ppm. Relationships between minerals are revealed by Pearson Correlation Coefficient (p&amp;lt;0.05). In addition, in this study, the ranking of macro and micro minerals and the relative values of some of these minerals (As/Se, Ca/Mg, Ca/Mn, Ca/Zn, Cu/Fe, Cu/Zn, K/Mg, Mn/Fe, Mn/ Mg, Zn/Fe) were presented.

Effects of antibiotic, acidifier, and probiotic supplementation on mortality rates, lipoprotein profile, and carcass traits of broiler chickens

Antimicrobial resistance is a significant issue, therefore it's relevant to assess the effects of antibiotics, acidifiers, and probiotic supplementation finding a good alternative to reduce the use of antibiotics in broiler production in rural areas of Bangladesh. Using randomized control trial, this 28-day study evaluated 360 Hubbard Classic broiler chicks divided into four groups: oxytetracycline-treated, acidifier-treated, Lactobacillus-based probiotic-treated, and control (no antibiotics, acidifiers, or probiotics). Each group was replicated three times with 30 birds each with adlibitum feeding. Body weight and feed intake were recorded weekly, and on 28th day, carcass traits and blood lipoprotein levels were evaluated. Results showed that in first and fourth weeks, the body weight gain significantly varied in probiotics and acidifier-treated birds than the control group (P < 0.001). The probiotic group had gained considerable increase in body weight (185.0 g vs 161.7 g and 1745.0 g vs 1592.7 g) than the control group. Notably, in the first week, the feed conversion ratio for the probiotic group was 0.76, but the antibiotic group's was 0.96 (P < 0.001). The weights of the drumstick (88.33 g) and liver (61.0 g) having probiotic supplements were substantially higher than those in the control group (77.0 g and 51.33 g, respectively) (P < 0.001). According to serum lipoprotein analysis, the probiotic and acidifier groups exhibited lower LDL levels (71.1 mg/dl and 69.8 mg/dl, respectively) and higher triglyceride levels (122.9 mg/dl and 135.4 mg/dl). These findings highlight the potential of probiotics and acidifiers as effective antibiotic alternatives, promoting carcass traits and lowering LDL levels in broilers in Bangladesh.

Growth performance of broiler chickens fed diets supplemented with amylase and protease enzymes individually or combined.

Feed additives that increase nutrient availability in feeds have gained a lot of interest. An experiment was conducted to determine whether amylase, protease, and their combined supplementation affected broiler performance. Two hundred eighty broiler chicks were selected and distributed randomly into 28 replicate pens with four treatment groups and seven replicates under a completely randomized design. A total of four diets were developed, having 0, 100, 100, and 100 + 100 g of control (AP0), amylase (A1), protease (P1), and amylase + protease (AP1)/ton of feed, respectively. Four replicates of each treatment were fed each diet. Each diet was randomly allotted to each group. Ad-libitum feeding was provided to the birds. The feeding program had starter and finisher diets. Upon completion of the experiment, three birds from each pen were slaughtered to analyze the carcass characteristics and organ weight. Differences were insignificant between 100 g/ton of amylase supplementation and FI, body weight gain (BWG), or feed conversion ratio (FCR) (p > 0.05). Supplementation with 10 0g/ton of protease did not significantly affect FI, BWG, and FCR (p > 0.05). Similarly, 100 + 100 g/ton of amylase + protease addition had no significant effect on FI, BWG, and FCR (p > 0.05). None of the treatments significantly affected carcass weight, abdominal fat percentage, dressing percentage, drumstick, wings, breast, and thigh weights (p > 0.05). In addition, there were no significant effects (p > 0.05) on the weight of the heart, liver, gizzard, and spleen. In conclusion, amylase, protease, and their combined supplementation at a rate of 100 g/ton of feed did not influence BWG, FI, FCR, carcass characteristics, or organ weight.

Effect of restricted feeding on hen performance, egg quality and organ characteristics of individual laying hens.

This study was to assess the impact of permanent or temporary restricted feeding on laying hen production traits, physiology, and egg quality. Two hundred and forty individually housed ISA Brown hens were monitored across 2 phases, assigned to 3 treatments: ad libitum feeding (ALF), temporary restricted feeding (TRF) and permanent restricted feeding (PRF), n=80 hens per treatment. In Phase 1 (P1), 22 to 40 weeks, the TRF and PRF hens were offered 115g of feed daily. In Phase 2 (P2), 41 to 46 weeks, the TRF hens were transitioned to ALF status while the ALF and PRF hens remained as in P1. From 35 to 40 weeks, eggs were collected once weekly from 15 hens per treatment and assessed for differences in albumen, yolk, and shell variables. At 45 weeks, 10 hens each from the ALF and PRF groups were euthanized and differences in organ characteristics were assessed. In P1, feed intake, feed to egg conversion ratio and body weight (BW) change were lower (P<0.01), while albumen height and Haugh unit were higher (P<0.01) in both PRF and TRF hen treatments compared to hens allocated the ALF treatment. In P2, TRF and ALF hens had a higher egg production and egg mass than PRF (P<0.01) than ALF. Body weight change in P2 was higher in TRF and similar in both ALF and PRF, while feed intake and feed conversion ratio were higher in TRF followed by ALF and least in the PRF treatment group (P<0.01). At 45 weeks ALF hens had a greater abdominal fat pad weight and fatty liver haemorrhagic syndrome lesion score compared to PRF. Restricting hens to 115g of feed per day from point of lay restrained BW, improved feed conversion ratio and albumen quality and reduced abdominal fat pad deposition and clinical signs of fatty liver haemorrhagic syndrome in individually housed laying hens.

Underfeeding Alters Brain Tissue Synthesis Rate in a Rat Brain Injury Model.

Brain injuries (BI) are highly disruptive, often having long lasting effects. Inadequate standard of care (SOC) energy support in the hospital leads to dietary energy deficiencies in BI patients. However, it is unclear how underfeeding (UF) affects protein synthesis post-BI. Therefore, in a rat model, we addressed the issue of UF on the protein fractional synthesis rate (fSR) post-BI. Compared to ad libitum (AL)-fed animals, we found that UF decreased protein synthesis in hind-limb skeletal muscle and cortical mitochondrial and structural proteins (p ≤ 0.05). BI significantly increased protein synthesis in the left and right cortices (p ≤ 0.05), but suppressed protein synthesis in the cerebellum (p ≤ 0.05) as compared to non-injured sham animals. Compared to underfeeding alone, UF in conjunction with BI (UF+BI) caused increased protein synthesis rates in mitochondrial, cytosolic, and whole-tissue proteins of the cortical brain regions. The increased rates of protein synthesis found in the UF+BI group were mitigated by AL feeding, demonstrating that caloric adequacy alleviates the effects of BI on protein dynamics in cortical and cerebellar brain regions. This research provides evidence that underfeeding has a negative impact on brain healing post-BI and that protein reserves in uninjured tissues are mobilized to support cortical tissue repair following BI.

Effect of ad libitum feeding of Holstein Friesian calves on immunological parameters and molecular stress on a transcriptional level

Our aim was to compare the health and performance of ad libitum (ADLIB) and restrictedly fed Holstein Friesian heifer calves. Calves were selected to ADLIB (n = 13) and control (n = 13) groups randomly. The period of ADLIB feeding lasted for 3 weeks after colostrum supplementation. The calves in the control group received the same milk replacer, which was supplied according to the restrained feeding schedule of the farm. There was no difference between the two groups in weight, weight gain or movement activity, furthermore in the values of glucose, albumin, total protein, BHB, AST, oxidant and antioxidant status incl. dROM, PAT and OSI. The IL8 gene had higher levels (non significant, p > 0.05) of expression in the ADLIB group during the first 20 days of life, which indicates that ADLIB feeding might potentiate a stronger immune response to environmental stress. The IGF1 gene showed increased expression in the ADLIB fed group at almost all time points, however the difference was already detected on the first day of the study, indicating the importance of individual differences even within the same breed. During the first 10 days INS expression was higher in the restricted group, followed by a shift by day 20 and after, when the ADLIB group showed a higher relative expression level. The observed values describe a trend that, although not significant (p > 0.05), would seem to indicate that ADLIB feeding might potentiate a stronger immune response to environmental stress.

Impact of growth trajectory on sexual maturation in layer chickens.

Recent studies showed that apart from photostimulation, metabolic triggers may independently activate sexual maturation and egg production in chickens. However, the origin, mode of action, and specific target(s) of this metabolic control remain unknown. Beyond body weight (BW), we hypothesize that body composition (BC) and associated specific metabolic signals are involved. Thus, this study was conducted to determine the BW and BC thresholds triggering spontaneous sexual maturation in layer pullets under different growth trajectories. Day-old Lohman LSL lite and Lohman brown lite chicks (n = 210 each) raised in brooding cages under ad libitum (AL) feeding until 8weeks of age were randomly allocated into individual cages and assigned to one of 3 experimental growth profiles; AL, breeder's target (T), restricted 20% below target (R), (n = 70 birds/profile/strain). Birds had free access to water throughout the trial. All hens were maintained on 10h of light (10lux) throughout the rest of the study. Blood and tissue samples were collected throughout the study to measure plasma estradiol (E2) concentrations and organ weights, respectively. Furthermore, carcasses were subjected to Dual-energy X-ray absorptiometry (DEXA) analyses. All analyses were completed with SAS using the MIXED procedure. Results show that R treatment slowed (p < 0.001) growth, delayed age at first egg (FE) and egg production (p < 0.001) and resulted in lower BW at FE (p < 0.001), lower ovary weight and number of follicles (p < 0.001) compared to AL in both strains, whereas, the strain significantly impacted body weight (p < 0.0001), ovary weight (p < 0.001), BW at FE (p < 0.001), age at FE (p < 0.001), egg production (p < 0.0001), E2 (p < 0.0001) and body composition (p < 0.05). For DEXA, AL feeding (p < 0.001) increased fat deposition compared to R. Furthermore, there was a positive correlation between plasma E2 and bone mineral content (p < 0.01) and bone mineral density (p < 0.01). In conclusion, feed allocation impacted growth and BC in a strain dependent manner which resulted in differing age at sexual maturation and egg production. Furthermore, a body fat threshold between 10% to 15% appears to be required for the occurrence of spontaneously sexual maturation in laying hens.

Digestibility Coefficients, Some Blood and Rumen Metabolites and Physiological Responses in Sudan Nubian Goats Subjected to Varying Levels of Feed and Water Restrictions

Three yearling uncastrated males of Sudan Nubian goatsranging in weight between 14 and 20 kg were used in a 3x3 latin squaredesign experiment with the objective of studying the effects of feed andwater restriction on dry matter intake (DMI), water intake, digestibility ofnutrients, rumen components, blood urea-N (BUN), rectal temperatureand respiration rate. The experiment consisted of three treatments:Adlibitum feed and water, feed restricted to 50% of adlibitum level withadlibitum water, water restricted to 50% of adlibitum level with adlibitumfeed. The results revealed no significant treatment effects on water intake,urine volume, rectal temperature and respiration rate. However, DMIdiffered significantly between treatments. The adlibitum provision ofwater resulted in insignificant higher water intake compared with theother two treatments. Rectal temperature was higher in water restrictedgoats than in feed restricted goats, whereas respiration rate decreasedinsignificantly with both feed and water restrictions. Feed and waterrestrictions had no effect on the digestibility coefficients of the variousnutrients. Water restricted goats showed, however, different trends exceptfor ether extract digestibility which tended to decrease. Treatment had nosignificant effect on the total digestible nutrients (TDN) values. The TDNtended to be higher with water restriction. There was no significanttreatment effect on rumen pH, ammonia-N (NH3 -N) and BUN. Feed andwater shortage did not significantly affect digestibility of the proximatecomponents but tended to slightly affect rumen metabolites, BUN,respiration rate and rectal temperature.

Nutritional and Physiological Responses of Sudan Desert Sheep and Nubian Goats to Water Restriction

This study was conducted to compare the nutrient utilization,concentration of some rumen metabolites, blood urea nitrogen and somephysiological responses to water restriction in Sudan desert sheep andNubian goats. Three yearling uncastrated intact males from each species,averaging 18.37 kg (for sheep) and 13.21 kg (for goats), were randomlyallotted to four treatments in a completely randomized block design witha 2 x 2 factorial arrangement of treatments to determine the effects ofanimal species and water restriction. Both species were offered either adlibitum feed and water or water restricted to 50% of ad libitum level withad libitum feed. Animal species resulted in a significant (P&lt;0.01) increasein dry matter intake, water intake, faecal dry matter and respiration rateand a decrease (P&lt;0.01) in water intake: dry matter intake ratio in sheepcompared with goats. Sheep showed also higher (P&lt;0.05) urine volumevalue than goats. Rectal temperature was not affected by animal species.Dry matter intake, faecal dry matter and urine volume decreasedinsignificantly with water restriction. However, water intake and waterintake: dry matter intake ratio decreased significantly (P&lt;0.01) withtreatment. Rectal temperature and respiration rate values were almostsimilar at the two levels of water intake. Species x treatment interactionshowed a significant (P&lt;0.01) increase in water intake in sheep comparedwith goats given water ad libitum. Water intake, however, decreased inboth species as a result of water restriction. Animal species induced asignificant effect on the apparent digestibility coefficients of dry matter,organic matter, crude protein, crude fibre, nitrogen free extract and totaldigestible nutrients. Ether extract digestibility did not differ betweenspecies. Goats had lower digestibility coefficients and total digestiblenutrients compared with sheep. Except for ether extract digestibility, the&#x0D; &#x0D; &#x0D; &#x0D; &#x0D; 233Sulafa M. H. Hassouna and Asim A. A. Lutfidigestibility coefficients of nutrients as well as total digestible nutrientsincreased with water restriction. Sheep had the highest nitrogen balancevalue. Nitrogen balance showed similar trend whether it is expressed as apercentage of nitrogen intake or digested nitrogen. Species x treatmentinteractions were not found for digestibility and nitrogen balance data.Rumen pH and ammonia nitrogen were not affected significantly byspecies. Water restriction did not induce a significant effect on rumen pHand blood urea nitrogen. A significant species and treatment effect onblood urea nitrogen and ammonia nitrogen, respectively, was recorded.

Effect of Red Pepper (Capsicum annuum L.) Oil Addition to Growing Quail Diets on the Performance, Slaughtering and Some Serum Characteristics

The aim of this study is to determine the effects of adding different levels of red pepper oil to quail diets on performance, slaughtering characteristics, and some serum parameters. For this purpose, a total of 300-day-old and mixed-sex quail chicks were randomly assigned to 5 treatment groups with 6 replications of 12 chicks each. Experimental animals were fed for 35 days with 5 different diets prepared by adding 0, 300, 600, 900 or 1200 mg/kg red pepper oil. During the experiment, quails were given ad-libitum feed and water. According to the results of the experiment, the effect of adding red pepper oil to the diet on the performance, carcass traits and visceral weights of the quails was statistically insignificant. Triglyceride and TAS concentrations of serum were significantly affected by the addition of red pepper oil to the diet. The highest triglyceride and TAS levels in the group with 300 mg/kg red pepper oil added; however, the lowest triglyceride and TAS levels were obtained in the groups supplemented with 600 mg/kg and 1200 mg/kg red pepper oil, respectively. According to this study findings, it can be said that the addition of red pepper oil to the diet in growing quails has a positive effect on serum triglyceride and TAS values when used between 300 mg/kg and 600 mg/kg in the diet, without adverse effects on performance and carcass characteristics.

Diurnal transcriptome landscape of a multi-tissue response to time-restricted feeding in mammals

Time-restricted feeding (TRF) is an emerging behavioral nutrition intervention that involves a daily cycle of feeding and fasting. In both animals and humans, TRF has pleiotropic health benefits that arise from multiple organ systems, yet the molecular basis of TRF-mediated benefits is not well understood. Here, we subjected mice to isocaloric ad libitum feeding (ALF) or TRF of a western diet and examined gene expression changes in samples taken from 22 organs and brain regions collected every 2h over a 24-h period. We discovered that TRF profoundly impacts gene expression. Nearly 80% of all genes show differential expression or rhythmicity under TRF in at least one tissue. Functional annotation of these changes revealed tissue- and pathway-specific impacts of TRF. These findings and resources provide a critical foundation for future mechanistic studies and will help to guide human time-restricted eating (TRE) interventions to treat various disease conditions with or without pharmacotherapies.

The Effect Of Giving Nano Herbal Biwa Leaf (Eriobotrya japonica Lindl.) Against Histological Description Of Kidney Rat That Induced By Alloxan

Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to abnormal insulin secretion and insulin action. Uncontrolled diabetes mellitus will cause various organ complications, one of which is diabetic nephropathy or kidney failure. The aim of this study was to determine the effect of nano herbal of biwa leaves (Eriobotrya japonica) on the structure of gromerulus, renal necrosis, proximal tubular constriction, kidney weight and kidney discoloration. This study uses an analytic study with a true experimental design in male rats (Rattus norvegicus) consisting of five treatments with each of six replications. This study consisted of a negative control group that was only given ad-libitum feed, a positive control injected with alloxan, and two groups injected with alloxan and given a 250 mg/kg and 500 mg/kg biwa nanoherbal. It was concluded that the nanoherbal of biwa leaves is effective enough to reduce blood glucose levels and is safe for consumption by diabetics because it does not damage kidney cells.

PSVI-12 Ghrelin Plasm Concentrations are Greater in High- vs low-RFI Hampshire Male Lambs within a Transient Surge of Secretion Just Before a Scheduled Meal Feeding

Abstract The aim was to explore potential differences on ghrelin plasm concentrations between efficient and inefficient lambs within a transient surge of ghrelin secretion just before a scheduled meal feeding. Six high- (256±19 g) and six low-RFI (-242±19 g) Hampshire entire male lambs (Age=168±2 d and BW=63±5 kg) were taken out of 25 individuals in a 56-d GrowSafe SystemsÒ feeding trial. After a 10-d adaptation to a once-daily ad-libitum feed offering (0900 h), at d11 three lambs from each RFI group continued the once-daily feeding schedule (FED), whereas feed was withheld from the other three lambs (FAST) from each RFI group for 24-h (d11 to d12). Blood samples were collected via indwelling jugular catheter. In d11, ghrelin plasm concentrations were determined by ELISA in all animals at 0800, 0840, 0900, 1000 and 1100, and d12 at 0900, 1000 and 1100 in the FAST group. A linear model for repeated measures was adjusted with PROC MIXED of SAS. Plasm ghrelin levels began to increase at 0800, and RFI groups reached significant difference (P &amp;lt; 0.05) within both FED and FAST groups at 0840, with the greatest difference at peak concentrations at 0900 h (3.9±0.2 vs 2.4±0.2 and 3.9±0.2 vs 3.0±0.2 ng/ml for high vs low RFI, respectively). In the descendent phase of the curve for the FED group, one hour after feeding, ghrelin plasm concentration levels continued to be greater (P &amp;lt; 0.05) for high RFI vs low RFI animals and reached basal levels by 1100 h. For the FAST group, ghrelin concentrations continued to increase averaging maximum concentrations at the end of feed restriction, 0900 h of d12, being 4.9±0.2 ng/mL for the high RFI animals and 3.5±0.2 ng/mL for the low RFI ones. In conclusion, high RFI lambs show more pronounced transient surge of ghrelin secretion just before a scheduled meal feeding than low RFI lambs.