Feasibility Of Tissue Engineering Research Articles

Restoration of a Critical Mandibular Bone Defect Using Human Alveolar Bone-Derived Stem Cells and Porous Nano-HA/Collagen/PLA Scaffold.

Periodontal bone defects occur in a wide variety of clinical situations. Adult stem cell- and biomaterial-based bone tissue regeneration are a promising alternative to natural bone grafts. Recent evidence has demonstrated that two populations of adult bone marrow mesenchymal stromal cells (BMSCs) can be distinguished based on their embryonic origins. These BMSCs are not interchangeable, as bones preferentially heal using cells that share the same embryonic origin. However, the feasibility of tissue engineering using human craniofacial BMSCs was unclear. The goal of this study was to explore human craniofacial BMSC-based therapy for the treatment of localized mandibular defects using a standardized, minimally invasive procedure. The BMSCs' identity was confirmed. Scanning electron microscopy, a cell proliferation assay, and supernatant detection indicated that the nHAC/PLA provided a suitable environment for aBMSCs. Real-time PCR and electrochemiluminescence immunoassays demonstrated that osteogenic markers were upregulated by osteogenic preinduction. Moreover, in a rabbit critical-size mandibular bone defect model, total bone formation in the nHAC/PLA + aBMSCs group was significantly higher than in the nHAC/PLA group but significantly lower than in the nHAC/PLA + preinduced aBMSCs. These findings demonstrate that this engineered bone is a valid alternative for the correction of mandibular bone defects.

Mandible reconstruction

The purpose of this article is to review current microvascular mandibular reconstruction techniques and recent literature on the advances of tissue engineering as they relate to mandibular reconstruction. Microvascular reconstruction continues to be the standard of care for complicated mandibular reconstruction in major ablative defects of the head and neck. Several recent modifications of current microvascular techniques offer significant improvement in the quality of reconstructions currently being performed. Advances in tissue engineering are currently not widely applicable clinically due to a number of factors; however, the technology offers promising advances in the management of mandibular continuity defects in the future. Microvascular reconstruction of the mandible represents the most significant advancement in technique in the current era. Tissue engineering offers a promising future to improve outcomes and decrease patient morbidity. Future investigations regarding this new technology will provide information on the utility and feasibility of tissue engineering in mandibular reconstruction.