An experiment was carried out with an aim of enhancing culture of Pangasianodon hypophthalmus. Juveniles with an average length of 13.35±0.14 cm and 17.52±0.16 g of body weight were subjected to various pH levels of 7.5 (Control), 6, 9 and 9.5 to determine their growth and hematological changes. The control group exhibited the highest growth in both length (14.70±1.04 cm) and weight (21.12±2.67 g), while the lowest length (13.87 ± 0.43 cm) and weight (18.92 ± 1.11 g) in pH 9.5. The highest (1.24 ± 0.15) and lowest (0.56 ± 0.15) value of SGR was noticed in control and pH 9.5 respectively. The highest value of PER (3.79 ± 0.38) was observed in control and the lowest (1.66 ± 0.31) in pH 9.5. The highest (2.59 ± 0.47) and lowest (1.11 ± 0.16) value of FCR observed in pH 9.5 and control respectively. The highest count (26.80 ± 1.01 (103/µl)) of TLC was observed in pH 9.5 and the least (22.26 ± 0.53 (103/µl)) in control. The highest count (2.47 ± 0.15 (106/µl)) of TEC was observed in control and the least (1.97 ± 0.23 (106/µl)) in pH 9.5. The highest value (9.42 ± 0.14 g/dL) of Hb was observed in control and the least (9.5 with 5.77 ± 0.56 g/dL) in pH 9.5. The highest value (29.06 ± 0.46%) of Ht was observed in control and the least (22.41 ± 2.11%) in pH 9.5. The highest value (124.93 ± 9.99 fL) of MCV was observed in pH 9 and the lowest (114.45 ± 11.98 fL) in pH 9.5. The highest value (32.42 ± 0.76 g/dL) of MCHC was observed in control and the lowest (25.73 ± 0.87 g/dL) in the pH 9.5. The study revealed that P.hypophthalmus juveniles showed significant difference (p < 0.05) between the control and treatments. Study inferred that alterations in the growth and haematological parameters were occurred at higher pH levels compared to lower pH levels.
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