Fatty Tissue Content Research Articles

The Relationship of Pharmacotherapy on Body Composition and Nutrition in Depressed Patients

Depression is recognized as one of the major mental illnesses. It often coexists with obesity, insulin resistance and related dietary conditions. There are many underlying causes of these conditions and one of them can be pharmacotherapy, used to treat depression. Therefore, the aim of this study was to assess the effect of pharmacotherapy on body composition of patients suffering from depression and the quantity and quality of food consumed by these individuals. This study involved 107 depressed patients, 32 of which were men treated with SSRI’s and SNRI’s and 75 were women treated with SNRI’s, NaSSA’s and SSRI’s. The test group was divided according to gender and the type of pharmacotherapy. The control group included 42 men and 62 women without any psychiatric treatment. All participants completed a questionnaire concerning their current diet and relevant medical history. Body composition was analysed using the BIA method. Data were analysed using Chi squared, Mann-Whitney U, Kruskal-Wallis tests and Spearman's rank correlation, where appropriate. Evaluation of the diet of depressed patients did not show any significant deviation from the recommended norms for consumption and the diet of the control group. However, in the group of men, BMI levels, waist circumference and SAT fatty tissue content depended on the calorific content of the diet and nutrient supply which correlated with SNRI treatment. In the group of women, treatment had little effect on calorific content of the chosen diet and nutrient intake, although significant differences in body composition were found, mainly among patients treated with NaSSA. Increased content of adipose tissue or higher BMI in men treated with SSRI’s may suggest that pharmacotherapy has a significant impact on the development of obesity and its complications in depressed individuals.

Diagnosis and management of Stafne bone cavity with emphasis on unusual contents and location

Stafne bone cavity (SBC) is a rare mandibular defect, and the diagnosis and management may be confused with the unusual contents and location. This report for the first time presented a case of SBC with a lymph node content. Meanwhile, a rare case of anterior SBC with fatty tissue content is reported. Typical SBCs with salivary glands content are asymptomatic and showed a well-shaped, ovoid, radiolucent image in the posterior mandible below the inferior alveolar canal in panoramic radiograph. The case of SBC with a lymph node content underwent surgical operation, and definite diagnosis is confirmed by histopathological examination. The case of anterior SBC with fatty tissue content is clarified by MRI confirmed the presence of the fatty tissue and avoided surgical exploration. Surgical interventions would be an unnecessary option in the management of SBC except for the doubtful cases or concomitant other pathologies.

The Influence of Body Mass Composition on the Postural Characterization of School-Age Children and Adolescents

Introduction In recent years a significant increase in the percentage of children with postural problems has been observed. It is necessary to focus on preventing the phenomenon and on analysis of existing postural defects. Aim The aim of this work was to assess the potential relationship between body mass composition and body posture in school-age children. Material and Methods 464 school-age children ranging from 6 to 16 years (234 boys and 230 girls) were qualified for the study. Body mass composition was assessed using the analyzer Tanita MC 780 MA. Assessment of body posture was performed using Zebris system. All the results were analyzed with statistical methods. The accepted level of significance was p<0.05. Results Analyses of the disparities between the girls and the boys showed statistically significant differences in all of the body mass components and in obliquity of the pelvis/shoulder. The boys were characterized by higher contents of muscle tissue (p<0.001), bone tissue (p<0.001), fatless tissue (p<0.001), and body water (p<0.001) as well as a greater obliquity angle (p=0.018). The girls, on the other hand, were found with higher content of fatty tissue (p<0.001). The children with lower content of muscle tissue (p=0.030), fatless tissue (p=0.030), water (p=0.030), and higher content of fatty tissue measured in kg (p=0.027) were characterized by greater pelvic obliquity. Conclusion The current study shows evidence that sex, as a strongly differentiating factor, determines body mass composition and the occurrence of suboptimal postures only in the pelvic area. Body mass composition differentiates body posture of the study group. The content of fatty tissue influences the occurrence of suboptimal postures in the scapula and pelvic area in the frontal plane. The content of muscle tissue is associated with faulty postures in the scapula and pelvic area in the frontal plane.

Changes in the content of catecholamines in blood lymphocytes after food consumption in young men in accordance with pronounced body fat component

We examined practically healthy young men (81 in the ages from 17 to 21 years), the students living in the city of Krasnoyarsk, for whom we defined their somatotypes according to V.P. Chtetzov scheme (1978) and component content of the body (fat, muscle and bone) by caliperometry technique. Catecholamine content (CC) in blood lymphocytes was analysed by luminescence-histochemical technique. Medical examination was carried out both in the fasted state, and in an hour after food intake (mixed breakfast). On an empty stomach CC content was practically the same in the young men of all the compared somatotypes. After food intake, CC statistically meaningful increase was revealed only in abdominal somatotype youngsters as compared to the same indices on an empty stomach (p=0.026). In abdominal somatotype we also found considerably higher CC content after food intake in comparison with the other somatotypes: chest (р=0.021), muscle (р=0.022) and inexplicit (р=0.029). Also, in abdominal somatotype, we revealed the highest absolute indices of fatty tissue content in body composition as compared to other somatotypes: chest (р=0.00000002), muscle (р=0.0002), inexplicit (р=0.000007). Considering CC level before and after food intake, we didn't mark any statistically relevant difference between the youngsters with different body mass indices (BMI). This finding could be caused by the following peculiarity of the BMI evaluation: on the one hand, the growth/weight ratio was calculated, on the other hand, body fatty tissue pronouncement wasn't. Besides, it is fatty tissue, which makes one of the most massive target tissues for CC, and therefore it greatly influences and regulates adipocyte functions. The research allows making the conclusion on the role of the expressiveness of body fatty tissue content as considerable factor, which influences CC content after food loads.

Effect of feeding various levels of poultry by-product meal on the blood parameters, filet composition and structure of female tenches (Tinca tinca)

Homogeneous background (age, sex, genetic lineage, culture conditions) was created to clearly demonstrate the impact of the tested dietary treatments. No feeds optimized for the rearing of the tench (Tinca tinca L. 1758) are available. Feeds are formulated to increase the growth rate or eliminate skeletal deformations. With the increasing prices of the basic components, fish meal (FM) and fish oil, poultry by-product meal (PBM) can be used. The aim of the study was to evaluate the effects of substitution of FM with PBM on the tench blood parameters, body composition and structure of skeletal muscles. Cage-reared female tenches (325 ± 18 g) were fed for 86 days with five types of feeds with 0% (control), 25.7%, 48.6%, 71.4% and 100% substitution of FM with PBM. No significant differences between the formulations were reported for weight, total length, fillet weight, visceral, liver, gonado-somatic, proximate composition and biochemical blood parameters. However, significant differences were found in the fillet profiles of fatty acids—an increase in the PBM content correlated with an increase in saturated and monounsaturated fatty acids and a decrease in n-3 PUFA and, generally, n-6 PUFA. Significant differences were also observed in, for example, the content of intramuscular fatty tissue and the level of organ lipidosis between the control variant (0% PBM) and that with 100% substitution. A sensory assessment indicated a higher gustatory value of the fillets in the case of feeds with 48.6% and 71.4% substitution.

Effects of Deferoxamine on Fat Graft Survival.

The most important problem in fat transplantation is the unpredictable rates of resorption. Deferoxamine (DFO) is an iron-chelating agent with many useful functions including stimulating angiogenesis and antioxidant nature. The purpose of the study is to evaluate the effects of DFO on fat graft viability in rat model. A total of 24 Wistar rats were divided into three groups and 0.5 g of the left inguinal fat pad was extracted. In control group, fat grafts were implanted to the parascapular area without performing any procedure. In sham group, they were implanted in 0.2 mL saline solution followed by serial saline injections for 1 month. In the study group, fat grafts were implanted in 0.2 mL saline solution and 300 mg DFO followed by serial DFO injections for 1 month. At the postoperative second month, fat grafts were taken back and sent for histopathologic examination. The weight measurements of biopsy specimens in the study group demonstrated significantly higher than in the other two groups. Inflammation and fibrosis rates were also found to be significantly higher in the study group compared with the other groups; however, no significant difference in the apoptosis rates was detected between the groups. Fat grafts enriched with DFO showed significant increase in fatty tissue content in the study group compared with the control and sham groups. DFO increases the fat graft survival in rats and it may be a useful addition in autologous fat grafting procedures to increase fat graft viability and obtain maximal long-term durability.

Proximate and fatty acid composition of liver and fatty tissue of patincatfish (Pangasianodon hypophthalmus)

The visceral storage fat and liver of patin catfish (Pangasianodon hypophthalmus) are normally discarded, which incurs cost and can cause environmental pollution. However, these may be potential sources to extract fish oil. The proximate and fatty acid compositions of liver and fatty tissue of patin catfish were investigated to evaluate the suitability of these by-products for extracting fish oil. Fat was extracted using a low temperature solvent extraction method. The average fat content of fatty tissue and liver of females were 77.64 and 11.71%, respectively, whereas in males this was73.23 and 9.59%, respectively. Fatty acids found in the extracted oil of these byproducts were C12:0, C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C18:4, C20:0, C20:1, C20:4, C20:5, and C22:6.The major fatty acids presented in these tissues were palmitic (C16:0), oleic (C18:1n-9), and linoleic acid (C18:2 n-6). The total amount of polyunsaturated fatty acids of liver from male and female patin catfish were 13.31 and 13.30%, respectively, whereas in the fatty tissue these were11.64 and 12.09%, respectively. The n-3 to n-6 ratios of liver and fatty tissue of females were 1.61and 0.95, respectively, whereas in male fish these were 1.31 and 1.05, respectively. Results of this study indicated that the liver and fatty tissues of patin catfish are suitable sources of fish oil specifically due to the presence of monounsaturated and n-3 polyunsaturated fatty acids.

P-wave characteristics and histological atrial abnormality

BackgroundFibro-fatty transformation is believed to be the leading cause of deteriorated atrial conduction; however, any direct assessment in relation to P-wave characteristics is lacking. We sought to assess P-wave morphology (PWM) and duration (PWD) in relation to histology of the atrial myocardium. ObjectiveAtrial specimens were collected from 11 patients who died from cardiovascular causes (7 men; median age 73years). MethodsTissue samples were taken at the level of superior and inferior PVs, center of posterior left atrial wall, terminal crest (CT) and Bachmann's bundle (BB) for assessment of fibro-fatty tissue extent. Standard 12-lead ECGs in sinus rhythm recorded during hospital stay were used for manual assessment of P-wave. Partial interatrial block (pIAB) was defined as a prolonged (≥ 120ms) and bimodal P-wave in any lead on 12-lead ECG. ResultsThe median PWD was 160 (120–200) ms. Fibrosis extent in CT highly correlated to PWD (r=0.914, p<0.001). The combination of fibrosis extent and fatty tissue in BB (16%, range 1%–41%), CT (18%, range 3%–47%) or superior PV (15%, range 6%–24%) correlated to PWD (r=0.627, p=0.039; r=0.795, p=0.003; and r=0.668, p=0.025, respectively). pIAB pattern was observed in 10 subjects; however, it was not associated with either fibrosis or fatty tissue content at any sampling location. ConclusionsOur findings further support causal association between PWD and the extent of structural abnormalities in the atrial myocardium and the major atrial conduction routes.

P-wave characteristics and histological atrial abnormality

Introduction: Fibro-fatty transformation of atrial walls is believed to be the leading cause of deteriorated atrial conduction, however any direct assessment of fibrosis extent in the major atrial conduction routes in relation to P-wave characteristics is lacking. We aimed at assessing P-wave morphology and duration in relation to histology of atrial myocardium. Method: Atrial specimens from 11 patients died from cardiovascular causes (7 men; median age 73 years, range 54-82) were collected. Tissue samples were taken at the level of superior and inferior pulmonary veins, center of posterior left atrial wall, Terminal Crest (CT) and Bachmann's Bundle (BB) and stained with Masson's trichrome for assessment of fibrosis and fatty tissue extent at all locations. Standard 12-lead ECGs in sinus rhythm recorded during hospital stay were retrieved from medical records, scanned for manual assessment of PWD and P-wave morphology. Partial Interatrial Block (pIAB) was defined as prolonged (≥120 ms) and bimodal P-wave in any lead on a standard 12-lead ECG. Results: The median PWD was 160 (range 120-200) ms. Fibrosis extent in CT highly correlated to PWD (r=0.914, p<0.001). The combination of fibrosis extent and fatty tissue in BB (16%, range 1-41%), CT (18%, range 3-47%) or SPV (15%, range 6-24%) correlated to PWD (r=0.627, p=0.039; r=0.795, p=0.003; and r=0.668, p=0.025, respectively). pIAB pattern was observed in 6 patients, however it was not associated with increased fibrosis or fatty tissue content at any sampling location. Conclusions: Our findings provide direct evidence of strong association between PWD and the extent of structural abnormalities in the atrial myocardium and the major atrial conduction routes, but do not support presumed association between fibrosis and ECG pattern of pIAB.

The level of vitamin D and its relationship with the amount of fatty tissue and adipocytokine content in the women of reproductive age

The patients with obesity are known to suffer 25-hydroxy vitamin D (25(OH)D) deficiency, but the relationship between these two conditions remains unclear. The blood 25(ON)D levels in practically healthy women were compared with the amount and distribution patterns of the fatty tissue and with the content of plasma adipocytokines. The absence or deficit of vitamin D was documented in 61.3% of the women included in the present study. They exhibited negative correlation between 25(ON) D levels and body weight. The patients with obesity were characterized by correlation between the 25(ON)D levels and waist circumference, body weight, BMI, and the amount of fatty tissue. The women of reproductive age with the reduced blood 25(ON)D level (OR 1.86 [0.88—3.95]; CI 95%) or 25(ON)D deficiency (OR 2.23 [1.03—4.80]; CI 95%) were at a higher risk of development of obesity compared with the women in whom the 25(ON)D levels were in excess of 75 nmol/l. The study confirmed correlations between adypocytokine levels and the amount of fatty tissue even though it failed to demonstrate a significant relationship between the levels of adipocytokines and vitamin D.

Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects

The complexity of stem cell lineage commitment requires studies to investigate the intrinsic and extrinsic regulatory events during differentiation. The objective of this long-term in vivo study was to investigate cellular differentiation and tissue formation of transplanted undifferentiated bone-marrow-derived mesenchymal progenitor cells (BMPCs) in combination with a medical grade polycaprolactone (mPCL) scaffold and to compare them to osteoblasts; a more differentiated cell type in a calvarial defect model.Tissue formation was assessed via histology, mechanical and radiological methods after 3 12, and 24 months. After 3 months our results indicated that transplanted mesenchymal progenitor cells were influenced by the niche of the host environment. Scaffold/BMPCs formed islands of bone tissue inside the defect area. However when the surrounding host calvarium contained a high content of fatty tissue, the fat content in the defect areas was also significantly higher. In contrast, defects repaired with scaffold/cOBs did not show this phenomenon. Analysis after 12 and 24 months confirmed these observations indicating that a predominantly fatty environment leads to adipogenic development in the progenitor group. Biomechanical data revealed that the tissue was less firm in the BMPC group compared to the cOB seeded group. Evaluation of cell plasticity in vivo has important consequences in clinical cell transplantation protocols. This study indicates that cell fate decisions are partially regulated by extrinsic control mechanisms of the immediate environment suggesting that induction of BMPCs into a specific lineage could be beneficial prior transplantation.

The Influence of the Vibroacoustic Environment Conditions on Functional Stability of Drivers

Despite new technological solutions and attempts to improve the working conditions during the operation of vehicles, city bus drivers are still exposed to negative impacts of noise and vibrations This research project was undertaken in response to the lack of holistic analyses of the environmental factors that might impact on the city bus drivers at work. No extensive major research was done to investigate the influence of the conditions at work on functional stability of bus drivers and basic physiological parameters of the human body (systolic and diastolic pressure, pulse rate, temperature in the ear channel, loading of postural muscles, blood circulation determined with the use of a thermovision camera, fatty tissue content, body mass and physical condition (squeezing and pressing exercises). The main purpose of this research program was to show the impacts that the conditions at work might have on bus drivers or to demonstrate that these impacts do no exist.

HRT affects skeletal muscle contractile characteristics: a definitive answer?

In this context, Ronkainen and colleagues (9) have cleverly used a design that minimizes the potential of genetic and/or physiological differences between users and nonusers (by using monozygotic twins), while optimizing the ability to tease out any HRT effects. The presented results are in favor of HRT having a significant positive impact on skeletal muscle functioning, given the strong relationship between muscle performance and HRT usage reported here. Through the robust design adopted here, this study therefore incrementally advances the field and in fact tips the balance toward a positive and measurable impact of HRT: HRT users have five times the levels of estrogen, walk 7% faster, jump 16% higher, and exhibit 8% greater relative lean tissue content and 5% smaller relative fatty tissue content compared with their nonusing counterparts.

Assessment of risk factors for osteoporosis and fractures in children with meningomyelocele

Our objective was to assess bone and muscular mass in children with meningomyelocele (MMC), and to analyze risk factors for osteoporosis and fractures based on densitometric examination. The study group included 30 patients (15 girls and 15 boys) with MMC, aged 6-17 years, treated in the Department of Pediatric Rehabilitation, University Hospital. Physiotherapeutic assessment and laboratory tests (serum parathormone, alkaline phosphatase levels, calcium, and phosphate levels, and urine calcium levels) were performed. Densitometry was measured by dual energy X-ray absorptiometry using a Lunar DPX-L apparatus. Lean mass (fat-free tissue content) and fat mass (% fat content) was evaluated. Femur fractures were the most common 12/30 (40%); 5/30 (17%) of the children with MMC had multiple fractures. The incidence of fractures correlated significantly with BMI and body fat content (p = 0.03) Children with MMC and fractures had a tendency toward higher BMI, despite the same absolute value of body mass, compared to those without fractures. Body fat levels were higher in MMC patients with fractures than in those without fractures (BMI R = 0.393, p = 0.03). Children with MMC and fractures had significantly higher 24 h calcuria values, despite normal renal function indices (p = 0.03). Low-energetic fractures in MMC children may result from metabolic disturbances that are a consequence of excessive renal calcium loss or excessive fatty tissue content.

Comparison of microstructural traits of &amp;lt;i&amp;gt;Musculus longissimus lumborum&amp;lt;/i&amp;gt; in wild boars, domestic pigs and wild boar/domestic pig hybrids

Abstract. The aim of the present study was to compare the histological structure of muscles in wild boars, pigs and wild boar/domestic pig hybrids by determining the percentage of different muscle fibre types and diameters and the intramuscular fatty tissue content of the Musculus longissimus lumborum (LL). The study involved 24 males representing three groups: wild boars (6 animals), pigs (12 animals: 6 of Polish Landrace and 6 of Duroc breeds) and wild boar/domestic pig hybrids (6 animals). Wild boar/domestic pig hybrids were obtained by crossing DU pigs with wild boars. Samples of the LL were frozen in liquid nitrogen. Frozen muscle samples were cut into 10- μm sections. These were later placed on a glass slide and stained using different histochemical reactions: NADH-TR and myofibrillar ATP-ase activity to distinguish muscle fibre types and Oil Red staining to determine the intramusuclar fatty tissue content. The highest proportion of slow twitch oxidative fibres and fast twitch oxidative fibres, being indicative of high meat value, was characteristic of wild boar muscles. The lowest diameters of all three muscle fibre types, associated with the largest number of fibres in the analysed area, suggest that the meat had the most delicate structure. Compared to wild boars, wild boar/domestic pig hybrids showed a decrease in the percentage of oxidative fibres and an increase in the percentage of glycolytic fibres. The diameters of all muscle fibre types in this group of animals were similar to those obtained in pigs.

Changes in body adiposity in girls and boys from the rural areas of East Poland over a time span of 20 years

Introduction: Excess adiposity is one of the major causes of a number of developing civilization diseases, hence increasing adiposity levels in populations of various countries over years have raised considerable attention worldwide.Objective: The study aimed to evaluate the character, dynamics and tendencies in adiposity changes in the population of children and youths from the rural areas of East Poland over the last 20 years.Material and methods: Body adiposity was evaluated using BMI values, fatty tissue content (in % and kg), and measurements of three skinfolds. The results obtained in the years 1999–2000 were compared with those reported for rural children and youths from that area in 1980.Results: Total body adiposity as well as adiposity of individual body parts were observed to increase considerably in the years 1980–2000. The highest adiposity gains were reported in the puberty period.Conclusions: The maintenance of the secular trend in body adiposity of children and youths from the rural areas of East Poland was observed in the experiment. The extent of those changes appeared to be differentiated depending on the adiposity measure applied, as well as sex and stage of ontogenetic development of subjects.Résumé. Introduction: L’excès d’adiposité étant l’une des causes majeures d’un certain nombre de maladies associées au développement économique, son accroissement dans les populations de diverses nations au fil des années, a soulevé une préoccupation dans le monde entier.Objectif: Cette étude a pour but d’évaluer le caractère, la dynamique et les tendances des changements d’adiposité dans la population des enfants et des adolescents des régions rurales de l’est de la Pologne au cours des vingt dernières années.Matériel et méthodes: L’adiposité corporelle a été estimée par les valeurs d’IMC, du contenu en tissus de réserve (en % et kg) et par les mesures de trois plis cutanés. Les résultats obtenus pour les années 1999–2000, ont été comparés avec ceux des enfants et adolescents de cette même région en 1980.Résultats: L’adiposité corporelle totale ainsi que l’adiposité de diverses parties du corps s’est accrue considérablement pendant les années 1980–2000. Les gains les plus élevés sont observés pendant la puberté.Conclusion: On a observé la poursuite de la tendance séculaire à l’accroissement d’adiposité des enfants et des adolescents de l’est de la Pologne. L’importance de ces changements est variable suivant la mesure effectuée ainsi que suivant le sexe et le stade de développement ontogénique des sujets.Zusammenfassung. Einführung: Extreme Adipositas ist einer der Hauptgründe für die Entstehung einer Anzahl von Zivilisationskrankheiten. Aus diesem Grunde hat in den vergangenen Jahren die Zunahme der Adipositas in Völkern verschiedener Länder für erhebliche Aufmerksamkeit gesorgt.Vorhaben: Die Studie zielte auf die Beurteilung von Charakter, Dynamik und weiterer Entwicklung von Adipositasveränderungen in den letzten 20 Jahren bei Kindern und Jugendlichen aus ländlichen Gebieten Ostpolens.Material und Methoden: Adipositas wurde über BMI-Werte, Anteil an Fettgewebe (in % und kg) und Messungen von drei Hautfaltendicken definiert. Die Ergebnisse aus den Jahren 1999–2000 wurden mit Daten von Landkindern und -Jugendlichen derselben Gegend aus dem Jahr 1980 verglichen.Ergebnisse: Gesamtkörperfettgehalt und der Fettgehalt einzelner Körperteile sind in den Jahren 1980–2000 offenbar erheblich angestiegen. Die höchste Zunahme an Adipositas wurde während der Pubertät beobachtet.Zusammenfassung: Es wurde in unserer Untersuchung ein stetiger säkularer Trend an Adipositas bei Kindern und Jugendlichen aus ländlichen Gebieten Ostpolens beobachtet. Das Ausmaß dieser Veränderungen schien in Abhängigkeit von der jeweils verwendeten Messmethode, vom Geschlecht und vom Entwicklungsstand des Probanden zu differieren.Resumen. Introducción: El exceso de adiposidad es una de las principales causas de una serie de enfermedades de las civilizaciones en desarrollo, por lo que el incremento durante años en los niveles de adiposidad en las poblaciones de varios países ha despertado una considerable atención en todo el mundo.Objetivo: El estudio pretende evaluar el carácter, la dinámica y las tendencias de los cambios de la adiposidad en la población de niños y jóvenes de las áreas rurales del este de Polonia, durante los últimos 20 años.Material y métodos: La adiposidad corporal se evaluó utilizando los valores del IMC, el contenido del tejido adiposo (en % y en kg) y las medidas de tres pliegues de grasa subcutánea. Los resultados obtenidos durante los años 1999–2000 se compararon con los señalados para los niños y jóvenes rurales de esa misma área en 1980.Resultados: Se observó que la adiposidad corporal total, así como la adiposidad de partes individuales del cuerpo, ha aumentado considerablemente entre los años 1980–2000. Los mayores incrementos en la adiposidad han correspondido al periodo de la pubertad.Conclusiones: En la investigación se observa el mantenimiento de la tendencia secular de la adiposidad corporal en los niños y jóvenes de las áreas rurales del este de Polonia. La magnitud de estos cambios parece ser distinta dependiendo de la medida de adiposidad considerada, así como del sexo y del estadio del desarrollo ontogénico de los sujetos estudiados.

A retinoid X receptor antagonist, HX531, improves leptin resistance without increasing plasma leptin level in KK-A y mice under normal dietary conditions

4-(5 H-2,3-(2,5-Dimethyl-2,5-hexano)-5-methyl-8-nitrodibenzo[b,e][1,4]diazepin-11-yl)benzoic acid (HX531) is a novel retinoid X receptor antagonist. This study provides evidence that HX531 improves leptin resistance without increasing plasma leptin levels in KK-A y mice, an animal model with high plasma leptin levels and leptin resistance. Under normal dietary conditions, 3 weeks of treatment with HX531 (0.03% and 0.06% food admixture) in KK-A y mice decreased plasma leptin levels in a dose- and time-dependent manner, in addition to decreasing body weight and mesenteric fatty tissue weight. To evaluate the effect of HX531 on leptin resistance, leptin was injected intraperitoneally in the KK-A y mice for 4 days after 1 week of treatment with HX531 (0.06% food admixture). This pretreatment with HX531 resulted in exogenously administered leptin causing a significant decrease in food intake. These results suggested that HX531 decreased plasma leptin levels accompanied by a decrease in fatty tissue content in the KK-A y mice and a simultaneous improvement in leptin resistance. This is the first report that HX531 improves leptin resistance without increasing plasma leptin level in KK-A y mice, under normal dietary conditions.

Visceral Obesity and Hemostatic Profile in Patients with Type 2 Diabetes: the Effect of Gender and Metabolic Compensation

Type 2 diabetes (T2DM) patients are characterized by a very high risk of cardiovascular diseases. Among the factors that are responsible for this phenomenon are abdominal obesity and hemostatic abnormalities. To examine the association of the markers of coagulation and fibrinolysis with the parameters of abdominal obesity and metabolic compensation in T2DM patients. 46 T2DM patients participated in the study: 24 men (mean age 61.1 +/- 7.9 years) and 22 postmenopausal women (mean age 62.6 +/- 8.7 years). In each patient the content and distribution of fatty tissue was measured by a dual energy X-ray absorptiometry method (DEXA). The central abdominal fat/gynoid hip fat (CAF/GF) ratio was calculated. The following hemostatic parameters were measured: fibrinogen (Fb), factor VII (fVII), antithrombin III (ATIII), C protein (pC), tissue plasminogen activator inhibitor (PAI-1) and alpha 2 antiplasmin (alpha2 AP). In addition, the biochemical indices of metabolic compensation were measured: HbA1c, glucose levels and lipids. Patients of both genders were divided according to median CAF/GF ratio. The activity of PAI-1 was significantly higher in women with CAF/GF ratio >or= 0.88 as compared to those with CAF/GF < 0.88 (2.64 +/- 1.28 vs. 1.61 +/- 0.27 U/ml, p < 0.05). The activity of ATIII was significantly lower in men with CAF/GF ratio >or= 1.17, as compared to those with CAF/GF < 1.17 (105.10 +/- 10.02 vs. 113.42 +/- 10.72 %, p < 0.05). There was a significant correlation between the CAF/GF ratio and the activity of PAI-1 in women (r = 0.30, p < 0.05). In addition, in men the CAF/GF ratio was negatively correlated with ATIII activity (r = -0.44, p < 0.05). Multiple stepwise regression analysis demonstrated independent association between the CAF/ GF ratio and the activity of PAI-1 (p < 0.001), and between the CAF/GF ratio and the activity of alpha2 AP (p < 0.01). There was an independent association between the concentration of HbA1c and the concentration of Fb (p < 0.001) and between triglycerides and the activity of fVII (p < 0.01). The results of our study show that the patients with T2DM and with higher markers of abdominal obesity measured by DEXA show fibrinolysis impairment and thrombinogenesis elevation compared to those with lower abdominal obesity markers. Independent factors determining hypercoagulation also include metabolic control and lipids. Hemostatic disorders place subjects with diabetes and abdominal obesity at risk of developing vascular complications.

Genetic Differences in Energy Partitioning in Growing Pigs

Energy partitioning was studied in pigs differing in potential for carcass lean growth and fatty tissue content from 25 to 105 kg body weight, by the means of repeated measures of detailed body composition and individual feed intake. In total, 141 pigs were included from the three genetic groups Norwegian Landrace (lean and efficient), Duroc and Landrace 2 LP (a fat and slow-growing selection line) (LLP). Individual feed consumption was registered, and detailed body composition measured repeatedly by computed tomography. Energy consumption [MJ metabolizable energy (ME) day -1 ] did not differ between the genetic groups. In general, about 40-50% of consumed energy was partitioned to growth. The genetic groups partitioned equal proportions of daily energy consumption to growth (ME GROWTH ) and maintenance (MEm) early and late in the growth period. From 50 to 85 kg body weight Landrace partitioned more to growth and less to maintenance compared with Duroc ( P <0.05). When considering partitioning of ME above maintenance, the genetically fat LLP had the highest net energy retention relative to the heat increment of feeding and was therefore the most efficient, Duroc was in an intermediate position while the lean Landrace had the lowest proportion. The partitioning of ME GROWTH to fatty tissue and carcass lean growth differed between the genetic groups ( P <0.001) according to genetic potential for carcass lean and fatty tissue gain. Increasing proportions were partitioned to fatty tissue growth with increasing body weight. The genetic groups partitioned equal proportions of ME GROWTH to non- fat visceral components (NFVC) growth. MEm varied between 0.65 and 0.68 MJ kg -0.75 day -1 . MEm increased with increasing weight of carcass lean and viscera ( P <0.01), more so in the modern breeds than in the LLP.

Effect of dietary protein source and lysine:DE ratio on growth performance, meat quality, and body composition of growing-finishing pigs.

The effects of four protein sources (soybean meal, sunflower meal, pea, and fish meal as the main protein source) and three apparent ileally digestible Lys:DE ratios (0.50, 0.43, 0.36 and 0.42, 0.36, 0.30 g Lys/MJ DE for 30 to 60 kg BW and 60 to 105 kg BW, respectively) in pig diets on growing-finishing performance, and carcass and meat quality traits were investigated. Eight individually housed animals per treatment received the diets from 30 to 105 kg BW at a level of 3.0 times maintenance requirements of energy. The ileal digestibility of protein sources was determined in a previous digestibility experiment. Protein sources showed no differences in growth performance from 30 to 105 kg BW. From 30 to 60 kg BW soybean treatment had lowest performance. The protein sources had no effect on lean meat percentage, liver weight, or meat quality (intramuscular fat content, pH at 45 min and 24 h after slaughter, drip loss, and meat color measured 24 h and 4 d after slaughter). The experimental diets formulated on the basis of similar apparent ileal digestible lysine content resulted in similar body composition regardless of the protein source used (P > 0.05). Reducing the Lys:DE ratio from 0.50/0.43 to 0.36/0.30 (by about 28%) reduced BW gain by 119 g/d from 30 to 60 kg and by 151 g/d from 60 to 105 kg BW. The gain:feed ratio increased by 82 g/kg in the first phase and by 47 g/kg in the second phase for the highest Lys:DE treatment compared with the lowest. Reducing Lys:DE ratio did not modify meat quality traits. A high Lys:DE ratio was associated with a high lean meat percentage. Differences between the medium- and low-Lys:DE groups were not significant. Lowering the Lys:DE ratio increased (P < 0.05) crude fat and fatty tissue content and decreased (P < 0.05) protein and muscle content in the body. Ash content and bone volume were not affected by Lys:DE ratio (P > 0.05). The chemical composition of the carcass can be predicted with moderate accuracy (R2 = 0.39 to 0.58) using volumetric composition data of previously frozen carcasses. In conclusion, similar growth performance, carcass and meat quality, and body composition can be expected if diet formulation is based on the apparent ileally digestible amino acid contents of feedstuffs, independent of dietary protein sources. Diminishing Lys:DE ratios reduce growth performance but do not modify meat quality traits. The chemical composition of the carcass can be predicted with moderate accuracy using the volumetric composition of thawed carcasses.