Muscle Fatty Acid Composition Research Articles

Dietary supplementation with succinic acid improves growth performance and flesh quality of adult Nile tilapia (Oreochromis niloticus) fed a high-carbohydrate diet

To evaluate the effects of dietary supplementation with succinic acid on growth performance, flesh quality, glucose, and lipid metabolism of Nile tilapia (Oreochromis niloticus) fed a high-carbohydrate diet (HCD), five iso-nitrogenous and iso-lipidic diets were prepared as follows: HCD (control group) consisting of 55% corn starch and HCD supplemented with 0.5%, 1.0%, 2.0%, and 4.0% succinic acid, respectively. Tilapia with an initial body weight of 204.90±1.23 g randomly assigned to 15 tanks with 3 replicates per group and 10 fish per tank fed for 8 weeks. Increasing dietary succinic acid supplementation resulted in significant second-order polynomial relationship in the weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR), protein efficiency rate (PER), viscerosomatic index, condition factor, and contents of muscular crude lipid and glycogen (P＜0.05). The hepatosomatic index, mesenteric fat index, liver glycogen content and crude lipid contents of the whole-body and liver demonstrated significantly linear and second-order polynomial relationship (P＜0.05). Quadratic curve model analysis based on WGR, SGR, PER, and FCR demonstrated that optimal supplementation with succinic acid in the HCD of Nile tilapia ranged from 1.83% to 2.43%. Fish fed with 1.0% succinic acid had higher muscular hardness (P＜0.05), increased the contents of alkali-soluble hydroxyproline in collagen, docosahexaenoic acid (DHA) and n-3 polyunsaturated fatty acid (n-3PUFA) in muscle (P＜0.05), and lower total fatty acid content in muscle (P＜0.05) compared with the control group. Compared to the control group, dietary supplementation with 1.0% succinic acid significantly increased the contents of total bounding amino acid (arginine, histidine, isoleucine, lysine, methionine, alanine, proline), total flavor amino acid content (free aspartic acid), the catalase (CAT) activity and total antioxidant capacity, and the mRNA relative expression levels of CAT, superoxide dismutase (SOD), and nuclearfactor erythroidderived 2-like 2 (Nrf2) in muscle (P＜0.05). Furthermore, succinic acid supplementation significantly up-regulated mRNA relative expression levels of glycolysis genes [hexokinase 2 (HK2), phosphofructokinase, muscle-A (PFKMA) and phosphofructokinase, muscle-B (PFKMB)], a key glycogen synthesis gene [glycogen synthase (GYS)], and lipid catabolism genes [carnitine palmitoyltransferase-1B (CPT1B), hormone sensitive lipase (HSL), and lipoprotein lipase (LPL)] (P<0.05), while down-regulating the mRNA relative expression level of fatty acid synthase (FASN) in muscle (P=0.004). In conclusion, dietary supplementation with 1.83% to 2.43% succinic acid improved muscle quality by increasing muscle antioxidant capacity and hardness, changing muscle amino acid and fatty acid composition, and regulating muscle glucose and lipid metabolism.

In vivo evidence of sea buckthorn relieving oxidative stress and improving immune performance of common carp (Cyprinus carpio L.).

Sea buckthorn has the functions of antioxidation, antitumor, anti-inflammation and regulating energy metabolism. In order to investigate the effects of sea buckthorn powder and sea buckthorn flavonoids on the antioxidant properties, immune function and muscle fatty acid composition of common carp, an oral feeding experiment was carried out. The administration of glucose significantly reduced the levels of glutathione and the activity of total antioxidant capacity enzyme in serum and hepatopancreas, while concurrently upregulating the level of malondialdehyde (MDA)(P < 0.05). Conversely, oral intake of sea buckthorn powder and flavonoids increased antioxidant enzyme activity and decreased MDA levels. In terms of antioxidant molecular indicators, sea buckthorn powder and sea buckthorn flavonoids significantly increased the mRNA levels of nuclear factor NF-E2-related factor (nrf2) in the hepatopancreas and muscle. Meanwhile, mRNA expression levels of downstream antioxidant-related genes (gr, cat, gpx, and sod) regulated by Nrf2 were also upregulated. In the immune aspects, the mRNA expression levels of proinflammatory cytokines, such as interleukin-6 (il-6), interleukin-1β (il-1β) and nuclear factor-κB (nf-κb), were reduced but the expressions of anti-inflammatory cytokines, such as growth factor-β (tgf-β) and interleukin-10 (il-10), were enhanced in the head kidney and spleen tissues after oral administration with sea buckthorn. In terms of muscle fatty acid composition, the ratio of n-3 polyunsaturated fatty acid (PUFA)/n-6 PUFA was notably higher after administering sea buckthorn flavonoids than that of the glucose group (P < 0.05). This study demonstrated that oral administration of sea buckthorn powder and sea buckthorn flavonoids significantly enhanced the antioxidant capacity and immune response and improved the muscle fatty acid compositions in common carp, and also mitigated the adverse effects of glucose treatment to a certain extent. © 2024 Society of Chemical Industry.

Enhancing farmed striped catfish (Pangasianodon hypophthalmus) robustness through dietary β-glucan.

β-glucan is a well-documented feed additive for its potent immunostimulatory properties in many farmed fish species. This study examined how it can also be a promising growth promoter, modulate antioxidant enzyme activities, and act as an anti-stress agent in striped catfish (Pangasianodon hypophthalmus). A 12-week feeding experiment was untaken to determine the effects of dietary β-glucan supplementation at graded levels (0, 0.5, 1.0, and 1.5 g kg-1). Measured indicators suggest that a dietary inclusion level of 1.5 g kg-1 β-glucan gave the highest positive responses: weight gain (120.10 g fish-1), survival (98.30%), and lower FCR (1.70) (P<0.05). Whole body proximate analysis had only revealed that crude protein was significantly affected by the dietary inclusion of β-glucan (P<0.05), with the highest protein content (19.70%) being in fish that were fed with 1.5 g kg-1 β-glucan. Although other inclusion levels (i.e., 0.5 and 1 g kg-1) of β-glucan did not enhance body protein content (P>0.05). The assessment of fatty acid composition in muscle, liver, and adipose tissues showed modifications with the inclusion of β-glucan. Antioxidative-related enzyme activities (inc. catalase, glutathione peroxidase, and superoxide dismutase) that were measured in the liver had higher levels when fed with β-glucan inclusion diets (P<0.05). Following the feed trial, fish were subjected to crowding stress treatment. It was subsequently found that catfish fed with β-glucan-based diet groups had lower levels of blood stress-related indicators compared to the control group with no dietary β-glucan. The use of 1.5 g kg-1 of dietary β-glucan resulted in the lowest measured levels of cortisol (43.13 ng mL-1) and glucose (50.16 mg dL-1). This study has demonstrated that the dietary inclusion of β-glucan can have functional benefits beyond the immunological enhancements in striped catfish. Furthermore, its use can increase production levels and mitigate the stress associated with intensive farming practices.

Study of the Interactions between Muscle Fatty Acid Composition, Meat Quality-Related Genes and the Ileum Microbiota in Tibetan Sheep at Different Ages.

The intestinal microbiota of ruminants is an important factor affecting animal production and health. Research on the association mechanism between the intestinal microbiota and meat quality of ruminants will play a positive role in understanding the formation mechanism of meat quality in ruminants and improving production efficiency. In this study, the fatty acid composition and content, expression of related genes, and structural characteristics of the ileum microbiota of ewes of Tibetan sheep at different ages (4 months, 1.5 years, 3.5 years, and 6 years) were detected and analyzed. The results revealed significant differences in fatty acid composition and content in the muscle of Tibetan sheep at different ages (p < 0.05); in addition, the content of MUFAs in the longissimus dorsi muscle and leg muscle was higher. Similarly, the expressions of muscle-related genes differed among the different age groups, and the expression of the LPL, SCD, and FABP4 genes was higher in the 1.5-year-old group. The ileum microbiota diversity was higher in the 1.5-year-old group, the Romboutsia abundance ratio was significantly higher in the 1.5-year-old group (p < 0.05), and there was a significant positive correlation with oleic acid (C18:1n9c) (p < 0.05). In conclusion, the content of beneficial fatty acids in the longissimus dorsi muscle and leg muscle of Tibetan sheep was higher at 1.5 years of age, and the best slaughter age was 1.5 years. This study provides a reference for in-depth research on the mechanism of the influence of the gut microbiota on meat quality and related regulation.

Improved muscle fatty acid composition and oxidative stability in lambs grazing on sainfoin pasture

As a mode of animal management, grazing on pasture has the potential to improve animal performance and provide healthy meat. However, there is little information about the effects of lamb meat quality grazed on sainfoin pasture. Therefore, the present study was conducted to compare the fatty acid composition and oxidative stability of growing lambs. The twenty-four lambs were randomly divided into grazing on sainfoin pasture (GS) or feeding indoors pelleted total mixed ration (FI). The results show that GS had the higher polyunsaturated fatty acids (PUFA), especially n-3 PUFA (P = 0.002), and beneficial for nutritional index of fatty acid. Corresponding that GS had lower the Thiobarbituric acid reactive substance (TBARS) in raw (P = 0.005) and cooked meat (P = 0.008). The GS had higher total phenols (P = 0.021), ferric reducing antioxidant power (FRAP) (P = 0.048) and α-Tocopherol of meat (P = 0.004). In conclusion, grazing on sainfoin pasture in lambs can improve muscle fatty acid composition and oxidative stability than feeding indoors.

Exposure to Imidacloprid under variable conditions disturbs the muscle fatty acid profile of a fresh water non target fish: Labeo rohita

Economy of Pakistan is heavily dependent upon agriculture and extensive use of pesticide is quiet common to enhance the crop yield. Imidacloprid is among the first choice pesticides in Pakistan and it has been reported that through run off along with water it ends up in water bodies affecting non target aquatic fauna. Through the present investigation, we are reporting the effects of Imidacloprid on the fatty acids composition of a non-target, commercially important carp: Labeo rohita. Fish were exposed to sub lethal concentration of Imidacloprid (120 mgL1) for 2, 4 and 8 days (short term) as well as for 16, 32 and 64 days (long term experimental conditions). Pesticide untreated controls were also maintained for each treatment. Following the specific Imidacloprid exposure, fatty acid composition (%) was determined in the muscle of all experimental groups by using gas chromatography. Fish exposed to Imidacloprid for 8 days had reduced Palmitic acid (p = 0.02) and elevated muscle Arachidic acid (p < 0.001) than control group. Labeo rohita exposed to the pesticide for 32 days had elevated muscle Oleic (p = 0.02) and Linoleic acid (p = 0.02) while fish exposed to Imidacloprid to 64 days had reduced muscle Palmitic (p = 0.04) and Oleic acid (p = 0.03). In conclusion, we are reporting that the exposure to sub lethal concentration of Imidacloprid disturb the muscle fatty acid composition of Labeo rohita that may affect its food quality. The effects were more pronounced under long term experimental conditions and were probably due to potentiating lipid peroxidation and disturbed fish metabolism upon Imidacloprid exposure.

Dietary LC-PUFA and environmental salinity modulate the fatty acid biosynthesis capacity of the euryhaline teleost thicklip grey mullet (Chelon labrosus).

The capacity to biosynthesise long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) depends upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. The presence of a Δ5/Δ6 desaturase enabling the biosynthesis of docosahexaenoic acid (22:6n-3, DHA) through the "Sprecher pathway" has been reported in Chelon labrosus. Research in other teleosts have demonstrated that LC-PUFA biosynthesis can be modulated by diet and ambient salinity. The present study aimed to assess the combined effects of partial dietary replacement of fish oil (FO) by vegetable oil (VO) and reduced ambient salinity (35ppt vs 20ppt) on the fatty acid composition of muscle, enterocytes and hepatocytes of C. labrosus juveniles. Moreover, the enzymatic activity over radiolabelled [1-14C] 18:3n-3 (α-linolenic acid, ALA) and [1-14C] 20:5n-3 (eicosapentaenoic acid, EPA) to biosynthesise n-3 LC-PUFA in hepatocytes and enterocytes, and the gene regulation of the C. labrosus fatty acid desaturase-2 (fads2) and elongation of very long chain fatty acids protein 5 (elovl5) in liver and intestine was also investigated. Recovery of radiolabelled products including stearidonic acid (18:4n-3, SDA), 20:5n-3, tetracosahexaenoic acid (24:6n-3, THA) and 22:6n-3 in all treatments except FO35-fish, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA and DHA from ALA is present and active in C. labrosus. Low salinity conditions upregulated fads2 in hepatocytes and elovl5 in both cell types, regardless of dietary composition. Interestingly, FO20-fish showed the highest amount of n-3 LC-PUFA in muscle, while no differences in VO-fish reared at both salinities were found. These results demonstrate a compensatory capacity of C. labrosus to biosynthesise n-3 LC-PUFA under reduced dietary supply, and emphasise the potential of low salinity conditions to stimulate this pathway in euryhaline fish.

Interrelation between gut microbiota, SCFA, and fatty acid composition in pigs.

The gut microbiota is a key player in the host metabolism. Some bacteria are able to ferment non-digestible compounds and produce short-chain fatty acids that the host can later transform and accumulate in tissue. In this study, we aimed to better understand the relationships between the microorganisms and the short-chain fatty acid composition of the rectal content, including the possible linkage with the fatty acid composition in backfat and muscle of the pig. We studied a Duroc × Iberian crossbred population, and we found significant correlations between different bacterial and archaeal genera and the fatty acid profile. The abundance of n-butyric acid in the rectal content was positively associated with Prevotella spp. and negatively associated with Akkermansia spp., while conversely, the abundance of acetic acid was negatively and positively associated with the levels of Prevotella spp. and Akkermansia spp., respectively. The most abundant genus, Rikenellaceae RC9 gut group, had a positive correlation with palmitic acid in muscle and negative correlations with stearic acid in backfat and oleic acid in muscle. These results suggest the possible role of Prevotella spp. and Akkermansia spp. as biomarkers for acetic and n-butyric acids, and the relationship of Rikenellaceae RC9 gut group with the lipid metabolism, building up the potential, although indirect, role of the microbiota in the modification of the backfat and muscle fatty acid composition of the host.IMPORTANCEThe vital role of the gut microbiota on its host metabolism makes it essential to know how its modulation is mirrored on the fatty acid composition of the host. Our findings suggest Prevotella spp. and Akkermansia spp. as potential biomarkers for the levels of beneficial short-chain fatty acids and the possible influence of Rikenellaceae RC9 gut group in the backfat and muscle fatty acid composition of the pig.

Effects of Aurantiochytrium acetophilum as a source of docosahexaenoic acid for juvenile Atlantic salmon (Salmo salar) on growth, nutrient apparent digestibility, tissue fatty acid composition and expression of lipid metabolism-related genes

Thraustochytrids are a group of unicellular marine protists that are noted for their ability to produce high amounts of lipids, especially docosahexaenoic acid (DHA), making them a valuable source for replacing fish oil in aquafeeds. Varying levels of Aurantiochrytrium acetophilum meal (TM) were evaluated as replacement of fish oil in extruded diets for juvenile Atlantic salmon on growth, nutrient apparent digestibility, fatty acid composition in muscle and liver and expression of genes associated with lipid metabolism. Four experimental diets were tested: a control diet without the inclusion of A. acetophilum meal and three experimental diets derived from the control diet, in which fish oil was progressively replaced by the addition of A. acetophilum meal at 2.5% (TM 2.5), 6.5% (TM 6.5) and 12% (TM 12) respectively. After 12 weeks of feeding, the growth, feed conversion, condition factor and survival of the fish were not affected by the inclusion of A. acetophilum in the diet. However, the 12% inclusion significantly decreased the viscerosomatic index of the fish and the digestibility of protein, lipids, ash and energy compared to the other experimental diets. The lipid content and fatty acid profile of the liver and muscle were significantly affected by diet composition. In both tissues, fish fed the TM 12 diet retained the highest levels of DHA. However, it was also observed that EPA concentrations lessened with increasing dietary content of A. acetophilum meal. Finally, the decrease in the expression of Δ5fad in the fish fed with the TM 6.5 and TM 12 diets showed that fish do not require a higher expression of this desaturase for DHA production, due to the high content of this fatty acid in A. acetophilum meal.

Assessing different oil sources efficacy in reducing environmental heat-stress effects via improving performance, digestive enzymes, antioxidant status, and meat quality

Adding oil to the feed of genetically improved broilers is necessary to provide energy requirements, in addition to enhancing metabolism, growth performance, immune response. This study aims to reveal the effect of adding different oil sources in the diets of broilers exposed to environmental heat stress on performance, digestibility, oxidative status, plasma lipids, fatty acids content, and meat quality. Six hundred twenty-five one-day-old broiler chicks were randomly distributed to five groups as follows: the first group fed a diet without oil (CON) as a control, while the second to the fifth group fed a diet containing soy oil (SO), corn oil (CO), olive oil (OO), and fish oil (FO), respectively. Results indicated a significant deterioration in growth performance, carcass traits, and oxidative state with a significant decrease in carcass quality in heat-stressed chickens fed the CON diet. Results showed increased growth, enhanced feed conversion ratio, and carcass dressing in broilers fed the oil-supplemented diet compared to the control diet, however, the digestive enzymes activity was not affected by receiving an oil-supplemented diet. The best performance was in chickens fed OO and SO, compared with FO and CO. Plasma aspartate aminotransferase (AST), and alanine aminotransferase (ALT) increased in broilers fed an oil-supplemented diet. Plasma high-density lipoprotein (HDL), and superoxide dismutase (SOD) remarkably increased in broilers fed OO, whereas the malondialdehyde (MDA) decreased compared to the other groups. Adding different dietary oil sources enhanced the breast muscle's fatty acid composition. Broiler diets supplemented with oils positively affected meat quality by enhancing color measurements, and TBA values, while the best were in chicken fed OO. It was concluded that adding dietary oil at 3% in the diets of broiler chicken exposed to environmental heat stress positively affected growth performance, enhanced oxidative status, and meat quality, best results were in broilers fed a diet that included olive oil.

Lipidomics and metabolomics reveal the enhanced mutton quality of Tan sheep under a standardized feeding regimen

One of the main factors affecting meat quality is the feeding regimen, which impacts muscle growth, fat accumulation, and fatty acid composition. This study characterized the lipid and metabolite profiles in Tan lamb using lipidomics and metabolomics to investigate the influence of standardized feeding and conventional grazing regimens on meat quality. We identified 813 lipids across five categories and 86 metabolites, including peptides, amino acids, carnitines, and nucleosides in Tan lamb. The standardized feeding regimen yielded fewer saturated fatty acids (SFAs), an increased n-6 polyunsaturated fatty acid (PUFA) content, and a higher PUFA/SFA ratio than the conventional grazing regimen. In addition, this regimen elevated the levels of bioactive peptides, vitamin B2 cofactors, and nucleosides. Analysis of purine metabolism and aminoacyl-tRNA biosynthesis revealed improved flavor and increased protein synthesis under the standardized feeding regimen. Therefore, the standardized feeding regimen enhances nutritional value and improves lamb flavor, making it suitable for Tan sheep feeding.

Sustainable utilization of unsaleable walnut kernel in substitution for corn grain in the diet of fattening lambs improves growth performance and meat quality.

As a cost-effective typical co-product of walnut processing; this study aimed to investigate the effects of feeding poor quality, unsaleable walnut kernel, also known as wasted walnut (WW) kernel, on growth performance, carcass characteristics, meat quality and fatty acid (FA) composition of fattening lambs. The experiment was conducted using 24 fattening lambs (27 ±1.07 kg initial BW) assigned to two total mixed concentrate-based diets, over a 80-day experimental period on a completely randomized design. Dietary treatments were: 1) WW (10% WW), and 2) CON (without WW). Average daily and total gain, and final weight increased with WW inclusion (P < 0.05). Also, a significant enhancement in feed conversion ratio (P < 0.05) and carcass cooler shrink loss (P = 0.03) were observed by feeding WW. Dry matter intake (DMI) was not influenced by feeding WW as did carcass prime cuts, meat, bone and fat-tail; however, the backfat thickness was increased (P = 0.01). WW inclusion reduced cholesterol and increased protein load in muscle tissue (P ≤ 0.05). Moreover, this inclusion led to higher concentration of vaccenic acid (VA), conjugated linoleic acid (CLA) and C20 polyunsaturated FAs (PUFA), total n3 and n6 PUFA in muscle and fat-tail (P ≤ 0.05). Due to enhanced animal performance, also chemical and FA composition of muscle and adipose tissue, WW can be included in fattening lamb diets as a rich source of protein and PUFA.

Genome-wide association study reveals candidate genes for traits related to meat quality in Colombian Creole hair sheep

Genome-wide association studies (GWAS) allow identifying genomic regions related to traits of economic importance in animals of zootechnical interest. The objective of this research was to conduct a genome-wide association study on meat quality traits using the Illumina OvineSNPs50 BeadChip array. The animals were sampled in the departments of Córdoba, Cesar, and Valle del Cauca. The genotypes obtained with the Illumina OvineSNP50 BeadChip microarray were analyzed SNP (single-nucleotide polymorphism) data to conduct a GWAS for pH and water-holding capacity (WHC) traits measured after 7 days of maturation, in the Longissimus dorsi (LD) muscle, in 167 Creole hair sheep of 12 months old belonging to Pelibuey (CHSP, n = 60), Ethiopian (CHSE, n = 44), and Sudan (CHSS, n = 63) breeds. The GWAS was done using a mixed linear model (MLMA) and based on the Ovis aries v3.1 genome. The CHSE showed the lowest meat juice release and, consequently, the highest water-holding capacity (WHC = 30.6 ± 0.1), suggesting that this breed has better performance in the meat industry compared with CHSS (WHC = 41.7 ± 0.1) and CHSP (WHC = 36.8 ± 0.1), since there is a relationship between WHC and juiciness. For the character pH, it was not possible to annotate genes related to meat quality, while, for the WHC, they have obtained 11 candidate genes associated (ELOVL2, ARAP2, LOC101102527, SHOC2, AIPL1, CSRNP3, IFRD, KDM8, NANS, DAPK1, IBN2, TPM2). Particularly, ELOVL2, ARAP2, IBN2, and TPM2 genes are involved in muscle contraction and fatty acid composition in sheep. In this study, we generated a baseline for GWAS related to meat quality traits in Colombian Creole hair sheep that can be used for future genomic selection plans.

Seasonal effect on fatty acid composition in phospholipid classes and triacylglycerols of male Capoeta umbla

The seasonal changes in muscle tissue, total lipids and fatty acid composition of phospholipids (PL), triacylglycerol (TAG), and phospholipid classes of male Capoeta umbla were investigated in this study. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) were identified as the major phospholipids (PLs) in the muscle tissue (PS). Triacylglycerols showed high contents of MUFA, 14:0, 16:1n-7, 18:1n-9, 18:2n-6, and 18:3n-3; while phospholipids presented high contents of AA, DHA, and 18:0. Myristic acid, 16:1n-7, 18:1n-9, monounsaturated fatty acids (MUFA), linoleic acid (18:2n-6), and linolenic acid (18:3n-3) were shown to be present in larger concentrations in TAG than in PL classes. In PL classes, 16:0, 18:0, arachidonic acid (20:4n-6) (AA), eicosapentaenoic acid (EPA) (20:5n-3), docosahexaenoic acid (DHA) (22:6n-3) and polyunsaturated fatty acid (ΣPUFA) were higher than the TAG fraction. The fatty acid composition of total lipid and lipid classes (TAG and PL) were affected by the seasonal variations and lipid fraction.

The use of sesame oil in sea bream feeds and its effects on growth and body chemical composition

As fish oil availability declines worldwide and its price rises, there is a growing need to engage in scientific investigations into alternative oil sources for incorporation into fish feeds. This study aimed to determine the effects of dietary sesame oil on the growth performance, feed utilization, and nutritional composition of sea bream (Sparus aurata). Twenty-five individual fish (initial mean weight: 32.38±0.27 g) were placed in each tank in triplicate. Four experimental fish meal-based (iso-proteic 49% and iso-lipidic 20%) diets were formulated; the control (C) group contains 100% of fish oil (FO) and varying amounts (20, 40, 60%) of sesame oil (SO). During the 75-day experiment, the fish were fed 3 times a day, and the mean ambient temperature and dissolved oxygen values were measured as 22.5±0.5°C and 6.8±0.3 mg/L, respectively. At the beginning of the experiment, 20 fish were sampled to determine their nutrient composition and somatic indexes. In the end, 4 fish were collected for the same procedures and 4 for each tank’s muscle and liver fatty acid compositions. At the end of the study, the S60 group exhibited the lowest final weight (FW) and feed utilization parameters. On the other hand, there were no statistical differences between FO, SO20, and SO40 in terms of FW. In addition, the SO40 group had the best feed conversion ratio (FCR) among the groups (P&lt;0.05). The whole-body lipid compositions of the groups were different. The highest lipid level was found in SO40 with 15.83±0.36%. Muscle and liver fatty acid composition was significantly affected by the fatty acid profile of experimental feeds. While total ω-6 fatty acids were higher in the SO groups than in group C, total ω-3 fatty acids were higher in the C group compared to the SO groups (P&lt;0.05). The ω-3/ω-6 ratios in the SO20 and SO40 groups showed similarity, while the highest ratio was observed in the control group (P&lt;0.05). As a result, according to data obtained from our study, it is predicted that 40% SO could be added to sea bream feed instead of FO in terms of growth performance, feed utilization, and nutritional composition.

Multi-omics analysis reveals the toxic mechanism of ammonia-enhanced Microcystis aeruginosa exposure causing liver fat deposition and muscle nutrient loss in zebrafish

Microcystis aeruginosa and ammonia pollution are two important environmental stress factors in water eutrophication. Herein, we simulated environmental conditions to investigate the effects of chronic exposure (single and combined) to M. aeruginosa and total ammonia nitrogen (TAN) on lipid metabolism and muscle quality in zebrafish. Our results showed that M. aeruginosa and TAN significantly induced lipid deposition and tissue damage in the liver of zebrafish. Liver transcriptomic analysis revealed that M. aeruginosa and TAN disrupted the balance in lipid synthesis, decomposition, and transport, ultimately leading to hepatic lipid accumulation. Moreover, exposure to M. aeruginosa or TAN alone resulted in decreased crude protein content and increased lipid content in muscle, as well as disrupted muscle fatty acid composition. Metabolomic analysis of muscle revealed significant alterations in metabolites such as glycerolipids, glycerophospholipids and fatty acids. The co-exposure of M. aeruginosa and TAN had a more significant effect on liver lipid dysfunction and muscle quality deterioration in zebrafish. These findings provide valuable insights into the potential risks and hazards of M. aeruginosa and TAN in eutrophic water bodies subject to Microcystis blooms, and can help inform effective strategies for monitoring and managing these toxins in aquatic ecosystems.

The Effects of Olive Cake Supplementation on Feedlot Performance and Longissimus Muscle Fatty Acid Composition of American Wagyu Steers and In Vitro Rumen Fermentation Characteristics

Wagyu cattle are well known for their greater intramuscular fat content which improves eating quality for consumers. These experiments were designed to investigate the impact of olive cake supplementation on Wagyu steer growth performance, longissimus muscle fatty acid composition, and in vitro rumen fermentation characteristics. We hypothesized that feeding with olive cake would improve animal performance and increase oleic acid (C18:1cis9) composition in the longissimus muscle. Experiment 1: Eighty-three American Wagyu steers (725 ± 10.7 kg) were used in this experiment. Steers were blocked by initial body weight (BW) and randomly assigned within blocks to one of two treatments. Treatments consisted of (1) control (basal ration with no olive cake) or (2) control diet + 5% supplemental olive. Steers were housed in feedlot pens (n = 4 steers/pen; 11 replicates/treatment) and fed a finishing diet typical for Wagyu cattle. Steers were individually weighed every 28 d throughout the 177 d experiment. Longissimus muscle samples were obtained for fatty acid analysis at the time of slaughter. Experiment 2: Rumen fluid from three beef steers (480 ± 10 kg) fitted with rumen canulae was used to investigate the impact of olive cake on in vitro rumen fermentation characteristics. Treatments consisted of (1) control (no olive cake) or (2) 5% olive cake. Results: Experiment 1: Steers receiving olive cake had a lower (p &lt; 0.05) final BW and DM intake when compared to steers receiving the control diet. Longissimus muscle C18:2 and C22:6 n-3 were greater (p &lt; 0.05) and C18:1cis9 tended (p &lt; 0.06) to be greater in steers receiving olive cake when compared to controls. Experiment 2: Dry matter, acid detergent fiber, and neutral detergent fiber disappearance and molar proportions of short chain fatty acids were similar across treatments. The weight percentage of C14:1 was greater in in vitro rumen fluid fermented with olive cake when compared to controls. All other long chain fatty acids were similar across treatments. Under the conditions of this experiment, feeding olive cake at 5% of the diet DM reduced live animal performance and had minimal impacts on longissimus muscle fatty acid composition and in vitro rumen fermentation characteristics.

Growth performance and fatty acids profile of common carp fed with raw black soldier fly larvae and honey bee drone brood

Abstract The present study aimed to compare the growth performance and liver and muscle fatty acid (FA) profile of juvenile common carp fed with raw black soldier fly (BSFL; Hermetia illucens) and honey bee drone brood (BDB; Apis mellifera), a novel insect in aquaculture, with a commercial carp diet (CCF). After 6 weeks, the weight gain and specific growth rate were significantly higher in fish fed with CCF, followed by the BDB-fed and BSFL-fed fish, with significant differences among groups. The essential FA contents in feeds were disproportionally mirrored in fish livers and muscles, suggesting feed-dependant biosynthesis ability in common carp. In conclusion, the results suggest that dietary FAs affect the muscle FA composition of common carp. Yet, the composition of dietary essential FAs content did not mirror the essential FA content in muscle, confirming the biosynthesis of essential FAs. The BDB appeared to be a viable alternative or supplement to CCF compliant with circular bioeconomy without compromising the FA profile of common carp muscles. Despite the low growth performance of fish fed with BSFL, this diet indicated good nutritional qualities regarding the FA profiles.

Genetic architecture and key regulatory genes of fatty acid composition in Gushi chicken breast muscle determined by GWAS and WGCNA

BackgroundFatty acids composition in poultry muscle is directly related to its tenderness, flavour, and juiciness, whereas its genetic mechanisms have not been elucidated. In this study, the genetic structure and key regulatory genes of the breast muscle fatty acid composition of local Chinese chicken, Gushi-Anka F2 resource population by integrating genome-wide association study (GWAS) and weighted gene co-expression network analysis (WGCNA) strategies. GWAS was performed based on 323,306 single nucleotide polymorphisms (SNPs) obtained by genotyping by sequencing (GBS) method and 721 chickens from the Gushi-Anka F2 resource population with highly variable fatty acid composition traits in the breast muscle. And then, according to the transcriptome data of the candidate genes that were obtained and phenotypic data of fatty acid composition traits in breast muscle of Gushi chickens at 14, 22, and 30 weeks of age, we conducted a WGCNA.ResultsA total of 128 suggestive significantly associated SNPs for 11 fatty acid composition traits were identified and mapped on chromosomes (Chr) 2, 3, 4, 5, 13, 17, 21, and 27. Of these, the two most significant SNPs were Chr13:5,100,140 (P = 4.56423e-10) and Chr13:5,100,173 (P = 4.56423e-10), which explained 5.6% of the phenotypic variation in polyunsaturated fatty acids (PUFA). In addition, six fatty acid composition traits, including C20:1, C22:6, saturated fatty acid (SFA), unsaturated fatty acids (UFA), PUFA, and average chain length (ACL), were located in the same QTL intervals on Chr13. We obtained 505 genes by scanning the linkage disequilibrium (LD) regions of all significant SNPs and performed a WGCNA based on the transcriptome data of the above 505 genes. Combining two strategies, 9 hub genes (ENO1, ADH1, ASAH1, ADH1C, PIK3CD, WISP1, AKT1, PANK3, and C1QTNF2) were finally identified, which could be the potential candidate genes regulating fatty acid composition traits in chicken breast muscle.ConclusionThe results of this study deepen our understanding of the genetic mechanisms underlying the regulation of fatty acid composition traits, which is helpful in the design of breeding strategies for the subsequent improvement of fatty acid composition in poultry muscle.

Effects of different forage proportions in fermented total mixed ration on muscle fatty acid profile and rumen microbiota in lambs.

The objectives of this study were to evaluate the effects of different forage proportions in the fermented total mixed ration (FTMR) on growth performance, muscle fatty acid profile, and rumen microbiota of lambs. Thirty 6-month-old small tail Han sheep × Ujumqin lambs with initial body weight (BW) of 27.8 ± 0.90 kg were selected for the test and divided into two groups of 15 sheep in each treatment (three pens per treatment and five lambs per pen) according to the principle of homogeneity. Two isoenergetic and isonitrogenous diets were formulated according to the NRC. The diet treatments were designed as (1) OH treatment containing 25% alfalfa hay and 35% oat hay, and (2) AH treatment containing 35% alfalfa hay with 25% oat hay. The forage-to-concentrate ratio for both diets was 65: 35 (DM basis). Three replicates were randomly selected from each treatment to determine growth performance, fatty acid profile and rumen bacterial communities in lambs. Results revealed no statistically significant (p > 0.05) differences in dry matter intake and average daily gain between the two diet groups. Cholesterol and intramuscular fat were significantly (p > 0.05) higher in the AH group, while no statistically significant difference (p > 0.05) was found in pH24 value. The muscle fatty acid compositions of lambs were obviously (p < 0.05) influenced by the diet treatments. Compared with the OH group, the C16:1, C17:0, and C20:3n6 contents were higher (p < 0.05) in the AH group, whereas the content of C18:1n9c, C20:1, C18:3n3, and C22:6n3 was obviously (p < 0.05) increased in the OH group. The monounsaturated fatty acid (MUFA) contents were significantly higher in the OH group, whereas no significant differences (p > 0.05) were detected in saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) contents among the two diet treatments. Bacterial composition was generally separated into two clusters based on principal coordinate analysis, and the OH group had a higher Shannon index. The relative abundance at the genes level of the Rikenellaceae_RC9_gut_group was obviously (p < 0.05) increased in the AH group and the relative abundances of Prevotella_1, Fibrobacter, and Bacteroidales_UCG_001_unclassified were obviously (p < 0.05) enriched in the OH group. Integrated correlation analysis also underscored a possible link between the muscle fatty acid compositions and significantly altered rumen microbiota. Overall, oat-based roughage in FTMR could promote a beneficial lipid pattern in the Longissimus lumborum muscles of lambs. These findings provide a potential insight into diet effects on fatty acid profile and the rumen microbiome of lambs, which may help make decisions regarding feeding.