Fat Depth Research Articles

The Multi-Loci Genotypes of the Myostatin Gene Associated with Growth Indicators of Intensively Fattened Lambs of Latvian Sheep.

Myostatin (MSTN), a member of the TGFβ superfamily, negatively affects skeletal muscle growth and controls muscle development at stages of pre-natal muscle growth. Marker-assisted selection with molecular markers of the MSTN gene can be used in animal husbandry for higher-quality and more economically profitable lambs. The study aims to identify multi-locus genotypes within the MSTN gene regions as molecular markers for fattening, feed efficiency, and carcass traits in Latvian sheep. This analysis was conducted on 76 intensively fattened male lambs representing six breeds in Latvia. Nine genotypes were identified based on four SNPs in the promoter and exon 1 regions, while six and four SNPs in introns 1 and 2 led to the formation of 14 and 10 genotypes, respectively. Six genotypes identified in the 3'UTR were significantly linked to traits such as birth weight, slaughter yield, muscle development, fat depth, average daily gain, dry matter intake, and feed efficiency. Genotypes of intron 1 were associated with slaughter yield, while genotypes of intron 2 were linked to fat depth, dry matter intake, and residual weight gain. The study concludes that MSTN gene SNPs can be used in selection to enhance sheep breeds in Latvia and improve economic outcomes for breeders.

Use of real-time ultrasound measurements of fat thickness and longissimus thoracis muscle characteristics for predicting body fat depots in crossbred hair ewes

This study aimed to predict body fat depots using ultrasound measurements (USM) of fat thickness and longissimus thoracis muscle characteristics in crossbred hair ewes. A total of 24 animals with a mean body weight (BW) of 37 ± 4kg and a body condition score of 2.39 ± 0.49 were used. USM was recorded 24h before slaughter and included: subcutaneous fat thickness (SFT), area (LTMA), amplitude (LTA), and depth (LTD) of the l. thoracis muscle and kidney fat thickness (µKFT). After slaughter, the internal fat (IF) was separated, classified, and weighed as mesenteric (MF), omental (OF), or pelvic fat (PF). The left half was then separated into subcutaneous and intermuscular fat (CF), and the muscle and bone tissues were weighed separately and adjusted to take account of the whole animal. Total body fat (TBF) was determined to be the IF plus the CF weights. LTA and LTMA correlated poorly to moderately with fat depots (0.37 ≤ r ≤ 0.74, P < 0.05). Other than CF, µKFT showed poor to moderate correlation with the other depots of body fat (0.44 ≤ r ≤ 0.75, P < 0.05). The regression model used to predict MF had r2 of 0.87 (RSD=0.14kg) and included BW and LTMA (P<0,05). OF and PF were predicted with r2 of 0.55 and 0.44, respectively, including µKFT (RSD = 0.20 and 0.17kg). IF was predicted by empty BW, LTMA, and µKFT with r2 as 0.81 (RSD=0.30kg). For TBF, EBW and LTMA explained 72% of its variation (RSD= 0.59kg). USM of fat thickness and l. thoracis muscle characteristics can improve the accuracy of predicting body energy reserves in crossbred hair ewes.

The liveweight, carcasses and meat quality of intact and castrated male lambs reared under a pasture-fed Australian production system

Context Intact male (ram) lambs are often discounted by meat processors because of their perceived defective meat quality. Aims This study quantified the effects of castration on the liveweight, growth, carcass parameters and the meat quality of male lambs. Methods Liveweight, growth and carcass data were collected from 133 ram and 132 castrated (wether) lambs reared under a pasture-fed production system. A total of 50 lambs were subsequently selected, balanced by final curfew liveweight and castration status, to investigate additional carcass, meat quality, consumer sensory panel scores and colour stability parameters. Key results It was found that total average daily gains for ram lambs were highest (242.9 ± 5.2 g/day; P = 0.002), resulting from their higher pre-weaning growth rate of 327.7 ± 9.7 g/day (P &lt; 0.001). Wethers were fattier, having higher estimated intramuscular fat (3.9 vs 3.3 ± 0.1%, respectively) and subcutaneous fat depth (14.7 vs 13.5 ± 0.4 mm, respectively) than ram lambs. Purge loss (P &lt; 0.030) and ultimate pH (P &lt; 0.035) were higher for the meat of wether lambs, but only after 32 days of wet ageing. Consumers scored the meat of wether lambs as significantly more tender (61.0 vs 54.6 ± 2.3%; P = 0.005) and of greater overall liking (65.1 vs 61.1 ± 2.0%; P = 0.044) than that of ram lambs. These, and a comparison of shear force, near infrared spectroscopy for intramuscular fat content and retail colour with consumer thresholds, suggest a general satisfaction with ram and wether lamb meat quality. Conclusions Collectively, there seems to be few tangible performance and meat quality advantages to the castration of male lambs. Implications Prime lamb producers must consider flock management and market preference when deciding on best practice.

Analysis of Physiological Oxygen Concentrations in Different Abdominal Fat Layers by Body Mass Index.

Physiological oxygen concentration in adipose tissue is closely linked to metabolic disorders such as chronic inflammation and insulin resistance. However, the nature of the variation in the oxygen levels of adipose tissue with body mass index (BMI) and depths of abdominal fat remain unclear. Therefore, this study aimed to elucidate the patterns of oxygen concentration in adipose tissue layers according to BMI. In this study, patients undergoing abdominal fat removal surgery were divided into the normal-weight (NW) or overweight-obese (OW) groups based on their BMI. Oxygen concentrations in abdominal superficial (sSAT) and deep subcutaneous adipose tissue (dSAT) were measured. The oxygen consumption rate, mean cell area, and capillary density in both tissue layers were compared between the two groups. Furthermore, the interaction between these three variables, BMI, and adipose tissue oxygen concentration, was analyzed using linear regression. A total of 42 patients were recruited in this study and we observed that oxygen concentration in the sSAT was significantly lower than in the dSAT, irrespective of BMI. In terms of the oxygen concentration in the dSAT, OW's was significantly lower than that of NW's. Linear regression analysis revealed a significant correlation between dSAT oxygen concentration and BMI, mean adipocyte area, and vascular density. Individuals who are obese have significantly lower oxygen levels in the deep abdominal adipose tissue, and this is influenced by BMI, adipocyte area, and capillary density. This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Establishing Consensus for Mohs Micrographic Surgical Techniques in the Treatment of Melanoma in Situ for Future Clinical Trials: A Modified Delphi Study.

Mohs micrographic surgery (MMS) is a promising treatment modality for melanoma in situ (MIS). However, variations in surgical technique limit the generalizability of existing data and may impede future study of MMS in clinical trials. A modified Delphi method was selected to establish consensus on optimal MMS techniques for treating MIS in future clinical trials. The Delphi method was selected due to the limited current data, the wide range of techniques used in the field, and the intention to establish a standardized technique for future clinical trials. A literature review and interviews with experienced MMS surgeons were performed to identify dimensions of the MMS technique for MIS that (1) likely impacted costs or outcomes of the procedure, and (2) showed significant variability between surgeons. A total of 8 dimensions of technical variation were selected. The Delphi process consisted of 2 rounds of voting and commentary, during which 44 expert Mohs surgeons across the United States rated their agreement with specific recommendations using a Likert scale. Five of eight recommendations achieved consensus in Round 1. All 3 of the remaining recommendations achieved consensus in Round 2. Techniques achieving consensus in Round 1 included the use of a starting peripheral margin of ≤5 mm, application of immunohistochemistry, frozen tissue processing, and resecting to the depth of subcutaneous fat. Consensus on the use of Wood's lamp, dermatoscope, and negative tissue controls was established in Round 2. This study generated 8 consensus recommendations intended to offer guidance for Mohs surgeons treating MIS. The adoption of these recommendations will promote standardization to facilitate comparisons of aggregate data in multicenter clinical trials.

Assessment of skeletal muscle dynamics and milk production across a 300-day lactation in multiparous dairy cattle

The objective of this study was to evaluate changes in longissimus dorsi muscle depth (LDD) across lactation (0 to 300 DIM) and identify the impact of low versus high muscle reserves immediately after parturition on body weight and body reserve changes as well as production variables across a 300-d lactation. Forty multiparous cows were classified as high muscle (HM; LDD > 5.0 cm; n = 18) or low muscle (LM; LDD ≤ 5.0 cm; n = 22) based on LDD measurements collected within 24 h of parturition. Body weights (BW) and ultrasound scans to assess LDD and back fat depth (BFD) were collected monthly from parturition until 300 DIM. Ultrasound scans captured and measured using available software. Blood samples were taken at 7, 150 and 300 DIM, and plasma was analyzed for markers of metabolic status by measuring insulin, nonesterified fatty acids (NEFA), creatinine, and 3-methylhistidine (3-MH). Milk yield was recorded daily and milk components were analyzed monthly. Data analysis was performed and the statistical models included the fixed effect of muscle group, time, their interaction, and the random effect of cow nested within muscle group with repeated measures using a first-order autoregressive covariance structure. Muscle group was not related with BW or BFD for any of the time points measured. Cows lost BW from 0 to 60 DIM and gained weight from 60 to 300 DIM. Similarly, BFD decreased between 0 to 90 DIM and increased BFD after 90 DIM until 300 DIM. A muscle group by time interaction was observed for LDD. The HM cows had more muscle at 0 DIM, indicative of treatment assignment (1.34 cm more), and 300 DIM (0.78 cm more) and tended to have more muscle at 60 DIM (0.66 cm more) compared with LM. After 240 DIM, both muscle groups began net accretion of muscle reserves until 300 DIM. No differences were observed for blood metabolites measured based on muscle group. However, there were significant time effects for creatinine, 3-MH, and NEFA concentrations, which reflected the observed changes in BFD and LDD measured in ultrasound scans. For statistical analysis of daily milk production, observations were grouped into 3 stages of lactation, early (0-60 DIM), mid (60-240 DIM), and late lactation (240-300 DIM). There was a muscle group by stage of lactation interaction, where in early and mid-lactation, HM cows produced, on average, 1.9 kg more milk/d; however, in late lactation, LM cows produced 1.8 kg more milk/d. Our results indicate that muscle reserves are depleted in early lactation, and accreted in late lactation, whereas BW and BFD started to increase by 90 DIM. Data also supports that cows with more extensive muscle depletion in early lactation had greater milk production, however, substantial muscle accretion in late lactation may result in reduced milk production.

Comparison of ultra-wide band microwave system and ultrasound in live cattle to predict beef carcase subcutaneous fatness

Ultrasound and ultrawide band microwave system (MiS) were directly compared in their ability to scan live cattle to predict carcase traits. Commercial beef cattle (n = 315) were scanned on farm 0–14 days prior to slaughter. Traits measured were subcutaneous fatness at the P8 site (over the gluteus muscle on the rump, at the intersection of a line through the pin bone parallel to the chine and perpendicular through the 3rd sacral crest) and subcutaneous fatness at the rib fat site (between 12th & 13th rib, ¾ of the length ventrally over the longissimus muscle). The precision of prediction of carcase traits was slightly better using MiS. MiS prediction of P8 fat depth had an average RMSEP of 2.48 mm and R2 of 0.65. The MiS could predict carcase rib fat with an average RMSEP of 2.28 mm and R2 of 0.56. The accuracy of prediction was very similar between the two technologies. When predicting P8, the average bias was smallest using MiS at 0.157 mm, but the average slope was smallest using ultrasound at 0.03 mm. When predicting rib fat, MiS had the smallest average bias at 0.204 mm, and smallest average slope deviation at 0.06 mm. The MiS predicted P8 and rib fat carcase traits with similar precision and accuracy as ultrasound.

Meat tenderness in Australian lamb: Data editing, environmental variation and their effects in genetic parameter estimation

Breeding for meat quality increases the value of lambs and requires reliable genetic parameters to achieve balanced genetic progress. Meat tenderness, accomplished by selecting for lower shear force, is an important eating quality trait because of its relationship with consumer satisfaction. Factors influencing shear force, include the pH and temperature decline post-mortem which can contribute towards higher shear force values and increased variation across contemporary groups. This study explored if genetic parameters for shear force change when post slaughter covariates and heterogeneous variance are corrected for, using data from 32,223 animals from different sheep breeds. Results showed that removing extreme individuals and contemporary groups with high mean shear force values reduced residual variance, followed by a smaller reduction in additive genetic variance and little effect on heritability. Results show that edited data performed better at predicting progeny performance and reduced potential bias introduced in the genetic evaluation due to data quality. The effect of including post-slaughter covariates in the genetic analysis was tested by estimating different model predictability through regression of estimated breeding values against offspring performance, showing that the model including hot carcass weight performed better followed by the one including both carcass weight and C-site fat depth. Our results highlight that historic and current in-plant recording practices do not provide the capacity to account for non-genetic factors associated with abattoir environment that might be impacting the ability to accurately calculate shear force breeding values. In that sense, genetic evaluation can be improved by applying more rigorous data editing.

PSIX-27 Ultrasound scanning of carcass traits in Katahdin lambs for predictive breeding value and marketing premium

Abstract Ultrasound scanning (USS) technique has been routinely used in performance testing of meat animals, such as swine and beef cattle. This study is to evaluate USS application in organic lamb production, selection, and the accuracy of carcass traits and market value prediction. Ewes (n = 60) were bred, pregnancy diagnosed at 40 d post-mating, and their suckling lambs were assigned to two groups, a non-creep feeding (control) and creep feeding (treatment) group. Each group was balanced with sexes and litter size when allocated into groups at 30-d of age. All lambs had unrestricted suckled their dams during pasture grazing or grazing with creep feeding supplementation until weaning. There were 28 control and 32 treatment group lambs presented at weaning 90 d of age. Lambs were continued to graze as two groups on fescue-based pasture or grazing with creep feeding supplementation till marketing at 120 d. Weaning body weight (BW), average daily gain (ADG), and post-weaning growth weight were significantly (P &amp;lt; 0.05) greater than control 24.0 kg, 226 g/d, and 28.0 kg for treatment group compared with control group at 21.3 kg, 196 g/d, and 25.3 kg, respectively. However, AGD was not significantly increased during the post-weaning creep supplementary feeding period. The ultrasound scanned carcass traits increase in creep feeding over control lambs at weaning and post-weaning measurements (Figure 1). The average ultrasound scanned back fat depth, loineye muscle depth, muscle width, and loineye muscle area were 2.2 mm, 17.9 mm, 45.6 mm and 5.8 cm2 at weaning age of 90 d; and 2.6 mm, 20.9 mm, 48.0 mm, and 7.0 cm2 at 120 d, respectively. The correlative associations (Table 1) between carcass traits and lamb BW, such as loineye muscle depth (LED), loineye muscle width (LEW), loineye muscle depth (LED) and loineye muscle area (LEA), and LEA were significantly improved (P &amp;lt; 0.05) with increased BW. However, there was no difference in these traits due to treatment except backfat depth, which was significantly higher for treatment and females. Creep supplementary feeding lambs during pre-weaning has increased ADG and weaning weight; therefore, will enhance market values of organically raised lambs. The results indicate that USS technique is worthy of application in meat lamb selection, post-weaning performance and market premium prediction.

349 The relationship of heart score with hot carcass weight and fat depth in Angus cattle

Abstract Congestive Heart Failure (CHF) is a cardiopulmonary condition observed in cattle raised in feedlots situated at elevations above 800 m. This condition leads to right-sided heart failure caused by increased pulmonary vessel pressures and cardiopulmonary remodeling, known as pulmonary hypertension (PH). In cattle experiencing PH, pulmonary arterial pressure (PAP) becomes abnormally high, increasing heart muscle load. This load stretches muscle fibers and enlarges the right side of the heart which can lead to heart failure and death. To assess the severity of PH, animals are assigned a heart score (HS) on a scale ranging from 1 to 5 at harvest. A score of 1 represents a normal heart, while a score of 5 indicates extreme heart remodeling. Understanding the relationship between hot carcass weight (HCW), fat depth (FD), and HS in Angus cattle will provide useful information on the impacts of this condition on feedlot performance and profitability. The objective of this study was to determine if a phenotypic relationship between HCW and FD with HS in Angus-influenced cattle. For this experiment, a total of 798 black, Angus-influenced cattle were used (245 steers, 553 heifers) that were fed near Greeley, CO. The HCW, FD, and HS data from each animal were recorded at harvest. Three general linear models were used to estimate the effects of HS on HCW and FD. Mean HCW and FD were 393.1 kg, (SD = 90.9) and 0.66 mm, (SD = 0.18), respectively, with an average HS of 2.08 (SD = 0.77). For all models, HCW and FD were fit as dependent variables, while lot and HS were included as fixed effects. Model 1 included all HS categories independently. Model 2 divided HS into 2 groups of normal (HS = 1 or 2) or remodeled (HS = 3+). For model 3 HS 3 was removed leaving only HS of 4 or 5 in the remodeled group. For both HCW and FD in Model 1, the overall effect of HS was not significant (P = 0.82 and P = 0.40, respectively). However, when comparing individual scores, animals with a HS = 4 were 15.0 kg lighter for HCW than individuals with a HS = 1 (P &amp;lt; 0.05). Conversely for FD, a non-significant difference (P = 0.74) of -0.33 mm was observed between animals with a HS = 4 versus a HS = 1. Grouping HS into 2 categories in Model 2 did change the outcome, yielding non-significant HCW and FD differences. However, when eliminating the HS = 3 data, a significant reduction in HCW of 16.9 kg (P = 0.05) was observed between the 2 HS groups, whereas non-significant effects on FD remained. These data suggest that at extreme levels of heart remodeling HCW may be extremely impacted negatively.

185 Effect of synchronized fixed-time AI and non-pregnant heifer delayed feedlot entry on reproductive performance and system economics

Abstract Heifer reproduction management research is being conducted at the NDSU–Dickinson Research Extension Center. Using crossbred heifers, the objective was to evaluate reproductive performance and economic efficiencies comparing a drylot synchronized fixed-time AI protocol with clean-up bulls to two grazing systems using a fixed-time AI protocol without clean-up bulls. Synchronization protocol:14-d CIDR-PG-GnRH (TAI 66 h ± 2 h). Fixed-time AI treatments: 1) Drylot Control (DLOT) with clean-up bulls, 2) Integrated diverse crop-heifer annual forage grazing (IAF, no clean-up bulls), and 3) Native range grazing (NR, no clean-up bulls). Following pregnancy ultrasound on d 85, non-pregnant heifers continued to bale-graze until late November, when they were transferred to the University of Wyoming SAREC feedlot, finished, harvested at Cargill Meat Solutions packing plant, Ft. Morgan, CO, and grid marketed. Data were analyzed using the MIXED procedure of SAS. Year 1 and 2 grazing days were 121, and 149, respectively. Synchronized TAI pregnancy rates yr 1 and 2 for DLOT, IAF, and NR were 59.4, 43.8, and 62.5%; 68.8, 46.9, and 34.4%, respectively. Total DLOT control pregnancy rates yr 1 and 2 were 90.6 and 100%. Grazing heifer feedlot performance Yr 1 did not differ for starting body weight (BW; 452 vs 436 kg, P = 0.29), ending BW (610 vs 600 kg, P = 0.74), gain (157 vs 164 kg, P = 0.69), average daily gain (ADG; 1.42 vs 1.48 kg, P = 0.70), dry matter (DM) feed intake (15.0 vs 14.7 kg, P = 0.73), F:G (4.81 vs 4.56 kg, P = 0.42), daily feed cost ($7.45 vs $9.47, P = 0.73), feed cost/kg BW gain ($3.11 vs $2.95, P = 0.51), and feed and yardage cost ($3.41 vs $3.24, P = 0.55). Year 2 grazing heifer feedlot performance for the IAF and NR were starting BW (501 vs 456 kg, P = 0.007), ending BW (670 vs 604 kg, P = 0.004), feedlot finishing gain (143 vs 123 kg, P = 0.05), ADG (1.08 vs 0.93 kg/day, P = 0.06), daily DM feed intake (20.1 vs 20.1 kg/d, P = 0.98), feed to gain ratio (7.13 vs 8.22 kg/d, P = 0.11), daily feed cost ($9.61 vs $9.54/d, P = 0.94), feed cost/kg BW gain ($4.05 vs $4.67/kg, P = 0.11), and feed and yardage cost/kg gain ($4.45 vs $5.13/kg, P = 0.092). IAF and NR Yr 1 HCW (P = 0.83), dressing % (P = 0.69), marbling score (P = 0.78) did not differ. Year 2, except for hot carcass weight (HCW; 379 vs 341 kg, P = 0.004), dressing pct (P = 0.99), fat depth (P = 0.29), marbling score (P = 0.94) did not differ. Systems 2-yr mean net return for DLOT, IAF, and NR were $592, $296, $379, respectively. Systems net return profitability was very good for all management comparisons, but reduced for IAF and NR due to IAF farming, finishing, and transportation costs, and NR finishing and transportation costs.

133 Effect of feeding two different feed additives (Prime Force and Optipartum C+) on finishing cattle performance and carcass characteristics

Abstract A feedlot study was conducted to evaluate the effect of two feed additives, Prime Force (Furst-McNess Company, Rockford, IL) and Optipartum C+ (AB Vista, Plantation, FL), on finishing cattle performance and carcass characteristics. In this experiment, steers [n = 320; 390 ± 9.5 kg of body weight (BW)] were used and four treatments were evaluated in a total of 32 pens, with 10 steers per pen and 80 steers per treatment. Treatments were arranged in a 2 × 2 factorial and included a control diet with no additives; a diet containing Prime Force targeted at 68 g/steer daily; a diet containing Optipartum C+, targeted at 20 g/steer daily; and a diet containing a combination of both products (68 g/steer Prime Force and 20 g/steer Optipartum C+). Cattle were fed for 151 d, but treatments were imposed the last 67 d before slaughter (i.e., fed control diet to all cattle until d 84). Performance is for the total 151-d feeding period. No interaction between feed additives was observed for performance or carcass traits (P ≥ 0.41), therefore main effects are presented. Feeding Prime Force increased final BW (P &amp;lt; 0.04) when compared with cattle not fed Prime Force. A tendency was observed for greater adjusted final BW (P = 0.06) and ADG (P = 0.08) for cattle fed Prime Force compared with cattle fed no Prime Force. Cattle fed Prime Force had numerically greater intake (P = 0.11) than cattle fed no Prime Force, therefore gain to feed (G:F) was not impacted (P = 0.54). Ribeye area and fat depth were not different (P ≥ 0.17) for cattle fed Prime Force versus cattle that had no Prime Force added to the diet, but marbling was numerically improved (P = 0.11) due to Prime Force. Carcass-adjusted final BW (P = 0.11) was numerically greater for cattle fed Optipartum C+ and ADG tended (P = 0.09) to be greater for cattle fed Optipartum C+ compared with cattle fed no Optipartum C+. Dry matter intake was not different (P = 0.54) for cattle fed Optipartum C+ versus cattle with no Optipartum C+ in the diet, therefore G:F was numerically improved (P = 0.13) by the increase in ADG for cattle fed Optipartum C+. Ribeye area, fat, and marbling were not different (P ≥ 0.30) between cattle fed Optipartum C+ and those that did not receive Optipartum C+ in the diet. Incidence of liver abscesses was not affected by feeding either additive (P = 0.40). Based on results from this study, adding Prime Force to a finishing diet tended to improve BW and ADG compared with cattle not fed Prime Force. Feeding Optipartum C+ tended to increase ADG and improve G:F due to equal DMI when compared with diets with no Optipartum C+ added.

Undegradable dietary protein limits growth and carcass yields in crossbred Boer kids fed Desmanthus hay or Rhodes grass (Chloris gayana) hay supplemented with urea and/or cottonseed meal

Context Low-protein tropical forages required protein supplementation, which might affect growth of meat goats. Aims This study was aimed to compare liveweight gain, carcass/non-carcass yields and meat quality in crossbred Boer kids fed isonitrogenous diets varying in undegradable (UDP) and rumen-degradable (RDP) dietary protein sources. Methods Twenty-five female crossbred Boer kids were randomly allocated into the following five dietary treatments: Rhodes grass (Chloris gayana) hay supplemented with urea (U), urea plus cottonseed meal (UCSM), cottonseed meal (CSM), gliricidia (Gliricidia sepium) (Gliri) or Desmanthus (Desmanthus leptophyllus) only (Desman). The diets were formulated to supply 135 and 195 g crude protein/kg DM for the two experiments. Experiment 1 lasted 78 days and the dietary UDP:RDP ratio (g/kg DM) was 35:102 (U), 37:100 (UCSM), 40:97 (CSM), 55:82 (Desman) and 46:91 (Gliri). Experiment 2 lasted 138 days and the dietary UDP:RDP ratio (g/kg DM) was 45:150 (U), 52:143 (UCSM), 58:137 (CSM), 83:112 (Desman) and 71:124 (Gliri) respectively. Data were subjected to one-way ANOVA and general linear model. Key results After 138 days of supplementation, the Desman goats had the highest liveweight gain (83 g/day) and heaviest cold carcass weight (12.1 kg) in Experiment 2. This was followed, in descending order, by CSM (58 g/day and 9.6 kg), UCSM (48 g/day and 7.8 kg), Gliri (41 g/day and 7.6 kg) and U (6 g/day and 6.0 kg). Heavier carcass weight was associated with larger eye-muscle area and fat depth at the 12th rib. Aus-meat Beef Colour Standard scores of 3–4 for the eye muscle of the Desman and CSM goats compared with scores of 1C-2 in the other goats meant that the darker meat in Desman and CSM diets was associated with heavier carcass weight and a more rapid rate of decline in carcass pH. Conclusions Crossbred Boer kids fed high dietary UDP had higher intakes of UDP, RDP, metabolisable protein (MP) and metabolisable energy (ME), even though an isonitrogenous diet was offered. The Desman kids had higher liveweight gain, heavier carcass yield, greater eye-muscle area, thicker fat depth and heavier mass of non-carcass components. Implications The higher liveweight gain and carcass/non-carcass yields for Desman goats than for others was associated with increased DM, UDP, RDP, MP and ME intakes.

Study on the consistency between CT hounsfield units and MRI evaluation of preoperative cervical paraspinal muscular fat infiltration in patients undergoing ACDF

ObjectiveTo explore the feasibility of applying CT Hounsfield Units (HUs) for the assessment of preoperative paraspinal muscle fat infiltration (FI) in different segments in patients who underwent anterior cervical discectomy and fusion (ACDF). To compare the consistency of preoperative paraspinal muscle FI evaluations using MRI and those via CT HUs in patients who underwent ACDF surgery.MethodsNinety-five patients (45 males and 50 females, aged 37‒71 years) who received CT and MRI examinations and underwent ACDF surgery were retrospectively analyzed. In the axial T2-weighted MR images at the median level of the C3/4, C4/5, and C5/6 segments, regions of interests (ROIs) were delineated along the boundaries of the cervical multifidus (MF) and semispinalis cervicis (Scer) muscles. Using the threshold tool in ImageJ software, areas of fat tissue and intermuscular septa within the ROI were quantified. The effective cross-sectional area (ECSA) for each side was obtained by subtracting the areas of fat tissue and intermuscular septa from the total ROI area. The ratio of the fat tissue area to the CSA was then calculated to determine the initial FI value. The depth of subcutaneous fat from the midline spinous process to the epidermis at the median plane of the C4/5 intervertebral disc was measured. The initial FI values were then divided by the depth of fat to determine the post-correction FI value. Using the Picture Archiving and Communication System (PACS), at identical segments and planes, ROIs were delineated using the same method as in MRI under a standard soft tissue window (width of 500 HU, level of 60 HU). The CT HU values were measured within these defined areas. The CT HU values from both sides are summed to obtain the total HU value for the segment. According to whether the measurement results of two sets of data follow a normal distribution, Pearson’s test or Sperman’s test was used to analyze the correlation.ResultsOn MRI, a statistically significant difference was observed in the post-correction FI only at the C3/4 segment compared to the other two segments (P < 0.05). No significant difference in the post-correction FI between the C4/5 and C5/6 segments was noted (P > 0.05). The CT HU results showed a substantial discrepancy between C3/4 and C4/5 segments and between C3/4 and C5/6 segments (P < 0.05), whereas no statistically significant difference was found in the CT HU value between the C4/5 and C5/6 segments (P > 0.05). The consistency analysis revealed a relatively strong correlation between the post-correction FI and CT HU values of the C3/4 and C4/5 segments. Furthermore, a strong correlation was detected in the variations in the measurement outcomes at the C5/6 segment.ConclusionPatients requiring surgical treatment for the cervical spine exhibit varying degrees of FI in paraspinal muscles across different locations and segments. Evaluating the degree of FI in the paraspinal muscles of the cervical spine through CT HU values is feasible. There is considerable consistency between the post-correction FI assessed under MRI and the measurements of CT HU values in evaluating the FI of paraspinal muscles in the cervical spine.

Influence of Increasing Carcass Weights on Pork Carcass Characteristics and Traditional and Alternative Fabrication Yields

The objective was to characterize the effects of increasing carcass weight on pork carcass characteristics and yields from traditional and alternative fabrication methods, as well as evaluate the size of novel retail cuts. Pigs (n = 85) were slaughtered and divided into 3 hot carcass weight (HCW) categories: Average (99 to 109 kg), Heavy (116 to 126 kg), and Very Heavy (134 to 144 kg). Loin muscle area (LMA) and back fat depth were measured on all carcasses. Paired right and left sides were fabricated traditionally and alternatively (shoulder separation at the 4th/5th rib), respectively. From the alternative side, the serratus ventralis (SV) was removed from the cellar-trimmed butt, and the triceps brachii (TB) was removed from the picnic shoulder. All individual primals and subprimals were weighed for yield calculations. Data were analyzed using the MIXED procedure in SAS including the main effect of weight class, with sex and sire line as fixed blocking effects. Differences were considered significant at P &amp;le; 0.05. Regardless of fabrication method, whole primal and subprimal weights increased (P &amp;lt; 0.01) in heavier carcass weight classes compared with lighter classes, but when expressed as a percentage of chilled side weight, these increases did not often translate into meaningful differences in distribution of carcass weight. While the alternative shoulder-loin separation reduced loin and belly length, loins and bellies from heavier carcasses weighed more than those from carcasses typically produced in the U.S. pork industry today. Serratus ventralis weight was increased (P &amp;lt; 0.01) approximately 0.28 kg from Average to Very Heavy, while the TB weight was increased (P &amp;lt; 0.01) approximately 0.24 kg from Average to Very Heavy. At heavier weights, alternative fabrication of carcasses yielded novel cuts from the shoulder including the SV and TB that were of size to warrant further exploration as retail offerings.

251 Effects of feeding increasing levels of dimethylglycine (DMG) on growth performance, livability, and carcass characteristics of commercial finishing pigs

Abstract This study aimed to evaluate the impact of increasing dietary levels of 78% sodium dimethylglycine (DMG) on growth performance, livability, and carcass characteristics of commercial finishing pigs. A total of 1,115 mixed-sex finishing pigs [initial body weight (BW) = 70.1 ± 1.8 kg] were housed in pens with 25 to 27 pigs per pen. Pens within block were assigned randomly to one of four dietary treatments containing 0 (Control), 0.25 (DMG_0.25), 0.5 (DMG_0.5) or 1.0 (DMG_1.0) g DMG/kg of feed with 11 replicates per treatment. This experiment extended over an 8-wk period until pigs were harvested at 125.4 ± 0.8 kg of BW. Data were analyzed using the MIXED procedure of SAS, and preplanned orthogonal contrasts were conducted to evaluate pairwise comparisons as well as linear and quadratic effects related to the increased levels of DMG. While no linear effect was observed, there was a tendency for a quadratic response in closeout feed efficiency (G/F; P = 0.08) and the percentage of pigs with morbidity (P = 0.06), with the most favorable responses found in pigs fed DMG_0.25 (Table 1). Pairwise comparisons between Control and DMG_0.25 treatments suggest that pigs receiving DMG_0.25 showed a 6% increase in closeout average daily gain (ADG; 1.01 vs. 0.95 kg/d; P = 0.02) and a 4% increase in closeout G/F (0.337 vs. 0.324; P = 0.05) compared with those fed Control diet. Furthermore, DMG supplemented at 0.25 g/kg tended to be associated with decreased mortality (P = 0.08), reduced morbidity (P = 0.09) and more pigs sold to the primary market (P = 0.10) compared with Control. In contrast, dietary supplementation of greater concentrations of DMG at 0.5 and 1.0 g/kg were not different compared with Control (P &amp;gt; 0.10). Carcass characteristics, including hot carcass weight, carcass yield, fat depth, loin depth, and fat-free carcass lean, were not different among treatments. The findings of this study suggest dietary supplementation of DMG at 0.25 g/kg of feed enhanced growth performance and livability in finishing pigs. However, feeding greater concentrations of DMG, up to 1.0 g/kg of feed, did not produce different outcomes compared with Control. These results indicate the potential benefits of DMG at specific inclusion rate in optimizing the production and health of finishing pigs.

252 Evaluation of phase feeding and complete diet blending at different standardized ileal digestible lysine levels on finishing pig growth performance, carcass characteristics and diet costs

Abstract A total of 2,160 mixed-gender pigs [PIC 337 × 1050; initial body weight (BW) = 24.9 ± 0.97 kg) were used in the 120-d study to compare feeding strategies (phase feeding vs complete diet blending) at different SID Lys levels (90 vs 100% of suggested requirement estimates) on finishing pig growth performance, carcass characteristics, and economics. Pens of 27 pigs were assigned to 1 of 4 treatments in a completely randomized block design with barn and initial BW as blocking factors resulting in 20 pens/treatment. The treatments included two feeding programs: a 5-phase feeding strategy at either 90% (Phase-90) or 100% of SID Lys requirement estimates (Phase-100) or blending of complete diets with pre-defined mixing proportions of a low and high SID Lys diet to meet 90 (Blend-90) or 100% (Blend-100) of the SID Lys curve requirement estimates for 23 to 127 kg pigs. Lysine levels were based on PIC 2021 requirement estimates. The 90% SID Lys diets were formulated to contain 4.38, 3.73, 3.06, 2.66 and 2.44 g SID Lys per Mcal NE for phases 1 to 5, respectively. The 100% SID Lys diets were formulated to contain 4.86, 4.15, 3.40, 2.96 and 2.71 g SID Lys per Mcal NE for phases 1 to 5, respectively. Pigs in phase-feeding strategies were fed on a feed budget with 21, 65, 67, 63 and 62 kg per pig for phases 1 to 5, respectively. Final BW and overall average daily gain (ADG; d 0 to 120) were not affected by the feeding strategy nor by diet SID Lys levels. In contrast, overall average daily feed intake (ADFI0 of pigs fed by diet blending was less than those fed by phase feeding (P = 0.002) resulting in increased gain to feed ratio (G:F; P &amp;lt; 0.001) for pigs fed by diet blending (table). The SID Lys levels did not influence overall ADFI or G:F. Hot carcass weight, carcass yield, percentage lean, fat depth and loin depth were not affected by the feeding program. Despite the decreased overall ADFI and increased G:F of pigs fed by diet blending, feeding strategy resulted in no significant differences in economic criteria except for reduced feed cost per kg gain at high price scenario (P = 0.049) for diet blending. With low diet cost, the 90% SID Lys level resulted in decreased feed cost per pig and feed cost per kg gain than 100% SID Lys (P &amp;lt; 0.001), but differences were not detected in income over feed cost (IOFC). In conclusion, diet blending at either 90 or 100% of the SID Lys requirement estimate reduced ADFI and increased G:F without impacting average daily gain (ADG) or carcass characteristics. At ingredient prices used in this study, feeding strategies at either 90 or 100% SID Lys did not significantly affect IOFC, but feeding 90% of SID Lys recommendation reduced feed cost.

162 Effect of molasses-based liquid supplement vs. corn-based dry supplement on beef cattle feedlot performance, carcass characteristics, and intestinal length

Abstract Molasses-based liquid supplements contain sugars that can be fermented into butyrate, the primary source of energy for gastrointestinal (GIT) epithelial cells and may improve gut health and enhance beef cattle performance. The aim of this study was to examine how a molasses-based liquid supplement, in contrast to a dry-based supplement, impacts the performance, carcass characteristics, and intestinal length of beef cattle. Crossbred steers [n = 60; body weight (BW) = 381.7 ± 1.3 kg] were divided into 10 pens (5 pens per treatment; 6 steers per pen) and were assigned to one of two treatment groups: dry, which consisted of a 10% ground corn-based dry supplement, or liquid, which included a 10% liquid molasses-based supplement. All the steers were fed a high-forage diet (56%) and were transitioned to a high-grain diet (7%) on d 1 of the experiment. The animals were on feed for an average of 122 d and had ad libitum access to feed and water. Steers were weighed every 21 to 28 d, and feed intake, average daily gain (ADG), and gain to feed ratio (G:F) were assessed both overall and for two distinct periods: the initial period (IP) from d 0 to 69, and the subsequent or second period (SP) from d 70 until slaughter. Intestinal length was measured at the time of slaughter. Data analysis was performed using the GLIMMIX procedure of SAS. Body weight and ADG did not differ between treatments in either period, or overall (P ≥ 0.41). However, it is noteworthy that feed intake was greater for steers that received the dry supplement during both periods as well as across the entire study (P ≤ 0.04). In terms of G:F, steers provided with the liquid supplement demonstrated a tendency for improved efficiency in the second period (P = 0.09) and an overall greater efficiency (P = 0.04) compared with those receiving the dry supplement. Intestinal length exhibited no difference for both the small and large intestine (P ≥ 0.57). For carcass data parameters no differences were observed for hot carcass weight, dressing %, or %KPH (P ≥ 0.24). Fat depth tended to be greater (P = 0.06), and yield grade (P = 0.01) and marbling score (P = 0.03) were greater for the steers fed the dry supplement. Rib-eye area was greater (P = 0.03) for the liquid supplement group. Therefore, these findings suggest feeding a liquid based supplement to steers on a 93% concentrate diet decreases intake, maintains BW, and improves feed efficiency and carcass leanness compared with steers fed a dry supplement. However, the improvement in efficiency as a result of feeding a liquid based supplement was not caused by an increase in absorptive capacity through increased intestinal length.

247 Evaluating the use of soybean hulls on growth performance, carcass composition, and digestibility when fed to grow-finish pigs

Abstract The addition of fiber to grow-finish diets is commonly utilized in swine production formulation as energy costs increase. The objective of this study was to determine the effects of feeding soybean hulls (SBH) at increasing levels to grow-finish pigs on growth performance, carcass characteristics, and apparent total tract digestibility (ATTD) of nutrients. Utilizing 192 grower pigs [PIC 337×1050, initial body weight (BW) = 21.99 ± 2.75 kg] that were randomly assigned to split sexed pens (n = 12 pens/treatment), pens were assigned to one of four dietary treatments and fed over three feeding phases for the estimated BW ranges of 22 to 50 kg, 50 to 86 kg, and 86 to 127 kg. Diets were formulated to represent conventional corn and soybean meal diets, containing increasing levels of SBH at: 1) 0% SBH (0%); 2) 7.5% SBH (7.5%); 3) 15% SBH (15%); and 4) 22.5% SBH (22.5%). Within phase, diets had equal SID Lys:ME and energy was allowed to float. Individual BW and pen feed disappearance were collected for each phase to calculate pen average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency gain to feed ratio (G:F). On d 56 of study and during phase 2, 32 gilts (n = 8) were placed into individual metabolism crates, where total urine and feces were collected for 72 h to measure ATTD of gross energy (GE), nitrogen (N), neutral detergent fiber (NDF), and acid detergent fiber (ADF), then returned to original pens. At the completion of study, carcass weight, backfat depth, and loin depth were collected. Data were analyzed with the fixed effects of dietary treatment by ANOVA, linear, and quadratic contrasts. Pen served as the experimental unit for performance and carcass characteristics and pig as the experimental unit for ATTD analyses. Overall, as SBH increased from 0% to 22.5%, ADG tended to decrease (linear P = 0.060) with no difference in ADFI (P &amp;gt; 0.05), resulting in an overall decrease in G:F (0.38, 0.37, 0.36, 0.35; linear P &amp;lt; 0.001; 0, 7.5, 15 and 22.5% SBH, respectively). Final BW tended to decrease (linear P = 0.069) and carcass weight decreased (102.9, 102.7, 99.7, and 96.5 kg, linear P &amp;lt; 0.001) as SBH increased in diet. Carcass fat depth (P = 0.014) and loin depth (P = 0.006) linearly decreased with increasing levels of SBH in the diet. Increasing levels of SBH from 0% to 22.5% decreased ATTD of GE and N (linear P &amp;lt; 0.001) and tended to increase ATTD NDF and ADF (linear P = 0.056 and P = 0.073, respectively). Altogether, these data suggest increasing dietary SBH in grow-finish pig diets decreases growth efficiency and the digestibility of GE and N. Further, feeding high inclusion rates of SBH negatively impacts carcass yield, backfat and loin depth.

Prediction of the carcass tissue composition of “Blackbelly” lambs using in vivo and postmortem measurements

Objective. Predict the tissue carcass composition of “Blackbelly” lambs using in vivo and postmortem measurements. Materials and methods. Twenty lambs with an average age and weight of seven months and 29.07±2.88 kg, respectively, were used. Before slaughter, the subcutaneous fat thickness, depth, width and Longissimus dorsi muscle area were measured with ultrasonography. After slaughter, the cold carcass and tissues: muscle, fat and bone weight, were recorded. In the carcass, the thoracic depth, length, perimeter, length and width of the leg, as well as the compactness index, were also measured and recorded. Correlation analysis and regression models were used to predict tissue carcass composition. Results. The carcass tissues were correlated with L. dorsi muscle depth (p≤0.05; r-values ranged from 0.67 to 0.80) and carcass compactness index (p≤0.05; r ranged from 0.54 to 0.75). The r2 for the prediction equations of the carcass tissue composition ranged from 0.71 to 0.78 for fat (p≤0.001). Conclusions. The use of in vivo and postmortem measurements allowed the prediction of tissue carcass composition of lambs, with moderate to high accuracy (r2 &gt;0.71≤ and ≤0.78).