Farm Dogs Research Articles

A pilot study to detect the effects of a green-lipped mussel (Perna canaliculus) nutraceutical on working farm dogs with musculoskeletal abnormalities using accelerometry

ABSTRACT Aims To obtain preliminary data on changes in gait from the use of a green-lipped mussel (Perna canaliculus) extract product in working farm dogs with musculoskeletal abnormalities using accelerometry. Methods: New Zealand working farm dogs (n = 32) with signs of musculoskeletal abnormalities were enrolled in a double-blinded, placebo-controlled cross-over study. Each dog was allocated to one of six groups to receive three trial substances (180 mg full fat green-lipped mussel extract (GLME180); 220 mg full fat green-lipped mussel extract (GLME220); placebo) in one of the six possible different orders. Each trial substance was administered orally once a day for an 8-week period, with a 4-week washout in between each. Dogs wore a collar-mounted triaxial accelerometer for the study duration. Diet and activity were not controlled. Accelerations were recorded continuously and analysed (n = 27) in 10-second activity epochs partitioned into daytime and night-time periods. Analysis of activity during the daytime period was limited to epochs when dogs were gaiting faster than a walk. The median and IQR of activity were determined for the daytime and night-time. Additionally, the 75th and 90th percentiles of daytime activity for each 24-hour period were determined. Mixed effects linear regression models were constructed to determine if each trial substance altered the response variables. Results During the daytime, the 90th percentile was higher when dogs were given GLME220 compared with the placebo (β coefficient 2.6; 95% CI = 0.25–4.94; p = 0.03). Dogs that started the trial with the GLME products had a higher 90th percentile activity compared with dogs that began with the placebo (β coefficient 26.26; 95% CI = 0.45–52.06; p = 0.046). The 75th percentile for activity was not affected by the GLME product. The daytime IQR was larger when dogs were given the GLME180 product compared with the placebo (β coefficient 1.25; 95% CI = 0.12–2.37; p = 0.03). Night-time median activity and the IQR was greater in dogs that started the trial with the GLME products than in dogs that began with the placebo. The night-time IQR for activity was greater for GLME180 than for the placebo. Conclusions Administration of a low dose of the GLME-containing product increased peak activity in working farm dogs with signs of musculoskeletal abnormalities and may improve their performance. Clinical relevance Even mildly affected working farm dogs might benefit from support of their musculoskeletal abnormalities, and this particular GLME-based product shows promise as an adjunct to other management strategies.

Molecular and serological prevalence rates of Neospora caninum infection in dogs from Jordan.

Neosporosis is a proven disease of farm animals and dogs caused by Neospora caninum. This cross-sectional study investigates N. caninum prevalence and seroprevalence among 268 dogs. Nc5 gene PCR was carried out on dog faeces and confirmed by sequencing. Seroprevalence was detected using an indirect fluorescent antibody test (IFAT). Three age groups, gender, locality (Amman, Irbid, and Zarqa Governorates), dog type (stray, pet, and breeding), place of living (indoor/outdoor), food type (raw/cooked), having diarrhoea, having abortion in the area, and having animals nearby were tested as independent variables for associations with positivity to N. caninum using univariate and multivariable logistic regression analyses. The true prevalence of N. caninum was 34.3% (95% CI 28.4, 40.5) using the Nc5-PCR test. The true seroprevalence rate of N. caninum among dogs in Jordan was 47.9% (95% CI 41.4, 54.5) using IFAT. The sequenced isolates of Nc5-PCR products (n = 85) matched three N. caninum strains, namely, NcHareGre (n = 70, 82.4%, 95% CI 72.6-89), NC MS2 (n = 14, 16.5%, 95% CI 9.3-26.1), and L218 (n = 1, 1.2%, 95% CI 0.03-6.4). The three strains were isolated previously from three different countries and continents. N. caninum shedding is associated with abortion among dogs and animals in the area (odds ratio = 3.6). In Amman and Zarqa, living indoors reduced seroprevalence at 0.45, 0.24, and 0.02 odds ratios, respectively. Jordan shares three molecular N. caninum strains with three different countries and continents.

Owner-Reported Health Events in Australian Farm Working Dogs.

Working farm dogs are an integral part of livestock farming in Australia but, currently, no studies have investigated their overall health. This study aimed to identify the prevalent diseases and injuries among these dogs, offering a baseline dataset. A total of 125 farm dog owners were recruited at a public working dog event and interviewed about the health of their dogs in the preceding 12 months. Information was provided about 526 dogs. Most dogs were Kelpies, and not neutered. Nine percent of the dogs had been sold, 6% had died, and 1% had been retired. Of the 444 remaining dogs, 24% had a health event in the past 12 months, while 13% had a health event that affected their work. The injuries (11%) mostly involved the musculoskeletal system or skin, while the non-traumatic illnesses (13%) commonly included arthritis. Forty-five percent of the injured dogs and 26% of the ill ones received veterinary treatment. The results indicate similarities to previous studies into working farm dogs. Given their unique challenges, further research is needed to ensure optimal care for farm dogs in Australia.

Reinfection of farm dogs following praziquantel treatment in an endemic region of cystic echinococcosis in southeastern Iran.

Cystic Echinococcosis (CE) as a prevalent tapeworm infection of human and herbivorous animals worldwide, is caused by accidental ingestion of Echinococcus granulosus eggs excreted from infected dogs. CE is endemic in the Middle East and North Africa, and is considered as an important parasitic zoonosis in Iran. It is transmitted between dogs as the primary definitive host and different livestock species as the intermediate hosts. One of the most important measures for CE control is dog deworming with praziquantel. Due to the frequent reinfection of dogs, intensive deworming campaigns are critical for breaking CE transmission. Dog reinfection rate could be used as an indicator of the intensity of local CE transmission in endemic areas. However, our knowledge on the extent of reinfection in the endemic regions is poor. The purpose of the present study was to determine E. granulosus reinfection rate after praziquantel administration in a population of owned dogs in Kerman, Iran. A cohort of 150 owned dogs was recruited, with stool samples collected before praziquantel administration as a single oral dose of 5 mg/kg. The re-samplings of the owned dogs were performed at 2, 5 and 12 months following initial praziquantel administration. Stool samples were examined microscopically using Willis flotation method. Genomic DNA was extracted, and E. granulosus sensu lato-specific primers were used to PCR-amplify a 133-bp fragment of a repeat unit of the parasite genome. Survival analysis was performed using Kaplan-Meier method to calculate cumulative survival rates, which is used here to capture reinfection dynamics, and monthly incidence of infection, capturing also the spatial distribution of disease risk. Results of survival analysis showed 8, 12 and 17% total reinfection rates in 2, 5 and 12 months following initial praziquantel administration, respectively, indicating that 92, 88 and 83% of the dogs had no detectable infection in that same time periods. The monthly incidence of reinfection in total owned dog population was estimated at 1.5% (95% CI 1.0-2.1). The results showed that the prevalence of echinococcosis in owned dogs, using copro-PCR assay was 42.6%. However, using conventional microscopy, 8% of fecal samples were positive for taeniid eggs. Our results suggest that regular treatment of the dog population with praziquantel every 60 days is ideal, however the frequency of dog dosing faces major logistics and cost challenges, threatening the sustainability of control programs. Understanding the nature and extent of dog reinfection in the endemic areas is essential for successful implementation of control programs and understanding patterns of CE transmission.

Retrospective evaluation of postoperative joint immobilization using a temporary calcaneotibial screw for medial or lateral tarsocrural joint instability in dogs.

To evaluate the use of a temporary calcaneotibial screw (CTS) to immobilize medial or lateral tarsocrural joint instability (TCI) in dogs. Retrospective study. Twelve dogs (including five active working farm dogs) with TCI. Medical records (January 2015-June 2023) were retrospectively reviewed for cases of TCI managed surgically including temporary joint immobilization using a CTS and external coaptation. Clinical data consisted of medical records and an online survey completed by the owner. Surgical techniques to address TCI included primary ligamentous repair, synthetic ligament reconstruction, or malleolar fracture repair. Immobilization with a CTS was employed for 6-8 weeks postoperatively. The online survey was completed for 10 dogs. All dogs exhibited good-to-excellent functional outcomes at the follow-up (median, 31 months; range, 4-66). All working farm dogs (5) were able to return to normal or substantial levels of their work. Four distinct complications were reported in three dogs including one CTS breakage and three bandage-related soft-tissue injuries. This retrospective study represents the first report of employing a temporary CTS for TCI in dogs. A temporary CTS was effective in immobilizing the tarsocrural joint for dogs with TCI and the postoperative complication rate in this study was relatively low. A CTS screw and external coaptation is a viable alternative to previously reported methods of tarsocrural joint stabilization.

The seroprevalence and risk factors for exposure to Neospora caninum and Neospora hughesi in Ontario broodmares

The seroprevalence and risk factors for exposure to Neospora caninum and Neospora hughesi in broodmares in Ontario were investigated. Sixty of the 219 (27.4%) study broodmares were seropositive for N. caninum and 65/219 (29.7%) for N. hughesi with cut-offs of ≥1:40 and ≥1:160, respectively. Thirty-one of 63 participating farms (49.2%) had at least 1 broodmare seropositive for N. caninum. Thirty-three of the 63 (52.4%) participating farms had at least 1 broodmare positive for N. hughesi. Risk factors for N. caninum included presence of farm dogs (OR = 6.70; 95% CI = 2.14–20.97; p = 0.001), and high stocking density (OR = 2.83; 95% CI = 1.27–6.30; p = 0.011). Presence of livestock, excluding cattle, was associated with reduced risk of exposure (OR = 0.17; 95% CI = 0.06–0.53; p = 0.002). The only risk factor for exposure to N. hughesi was feeding hay on the ground in the paddock (OR = 4.31; 95% CI = 1.65–11.22; p = 0.003). This study demonstrated widespread exposure to Neospora spp. in broodmares in Ontario.

Where did my dog go? A pilot study exploring the movement ecology of farm dogs.

Movement ecology is important for advancing our comprehension of animal behavior, but its application is yet to be applied to farm dogs. This pilot study uses combined GPS and accelerometer technology to explore the spatial patterns and activity levels of free roaming farm dogs, Canis familiaris (n = 3). Space-use distributions and range sizes were determined to compare locations visited across days and between individuals, as well as in relation to specific areas of interest. Individual activity levels were analyzed and compared within and between dogs. Space-use patterns and range sizes showed variation among the dogs, although substantial similarity in overall spatial distributions were observed between each pair. Among the dogs, the extent of spatial distribution overlap between days varied, with some individuals exhibiting more overlap than others. The dogs allocated different amounts of their time close to landscape features, and to slow-, medium-, and fast movements. This study demonstrates the potential of using automated tracking technology to monitor space-use and interactions between dogs, livestock, and wildlife. By understanding and managing the free ranging behavior of their farm dogs, farmers could potentially take steps to improve the health and wellbeing of both their dogs and their livestock, limiting disease spread, and reducing the possibility of related economic losses.

Bluetongue Virus Infection in Farm Dogs Exposed to an Infected Sheep Flock in South Africa

Bluetongue Virus Infection in Farm Dogs Exposed to an Infected Sheep Flock in South Africa

The occurrence of the carbapenemase gene, blaNDM-5, on a transmissible IncX3 plasmid in multidrug-resistant Escherichia coli isolated from a farm dog

In-depth phenotypic and genomic analyses on a carbapenem-resistant Escherichia coli isolate, recovered from the faeces of a farm dog in Lebanon, focusing on its antimicrobial resistance (AMR) patterns and the underlying resistome. E. coli strain EC-106 was identified using MALDI-TOF-MS. Analyses using Carba NP, immunochromatographic assay NG Carba5, and other antimicrobial susceptibility testing were performed. Whole-genome sequencing (WGS) using the Illumina technology and different software available at the Center of Genomic Epidemiology wwere used to predict the resistome, sequence type (ST), plasmid types, and virulence genes. Susceptibility testing revealed that E. coli EC-106 was multi-drug resistant, including against newer antimicrobials such as imipenem-relebactam (MIC = 16 µg/mL), meropenem-vaborbactam (MIC = 16 µg/mL), and ceftazidime-avibactam (MIC > 32 µg/mL), but remained susceptible to aztreonam (MIC = 0.12 µg/mL), aztreonam-avibactam (MIC = 0.06 µg/mL), and cefiderocol (MIC = 0.5 µg/mL). WGS analyses showed that E. coli EC-106 carried 13 acquired resistance genes associated with resistance to β-lactams (blaNDM-5 and blaTEM-1B), aminoglycosides (aac(3)-IId, aph(3')-Ia, aadA1, and aadA2), tetracyclines (tetA), amphenicols (partial catA1), macrolides (mphA), sulphonamides (sul1 and sul3), trimethoprim (dfrA12), and quaternary ammonium compounds (partial qacE). The blaNDM-5 was located on an IncX3 plasmid. The isolate was predicted to be a human pathogen (92.9%) and belonged to ST1011. To our knowledge, this is the first report of the detection of an IncX3 plasmid carrying the blaNDM-5 gene in animals in Lebanon, highlighting the severe AMR challenges in the country. Taken together, our current and previous findings suggest that blaNDM-5 might be spreading in different hosts and genetic backgrounds across clinical and non-clinical settings.

Prevalence of canine herpesvirus 1 and associated risk factors among bitches in Iranian breeding kennels and farms.

Canine herpesvirus 1 (CHV-1) is an important cause of death in newborn puppies and of fertility problems in adult dogs. Identification of risk factors may help to reduce infection rates and alleviate concerns for dog owners and breeders. This study was designed to screen for CHV-1 infection in bitches of breeding kennels and farms in Iran and relate this to possible risk factors. A total of 63 vaginal samples were collected from dogs in 5 breeding kennels (n=47) and from 7 farms (n=16). Real-time polymerase chain reaction was used to detect the CHV-1 specific glycoprotein B (gB) gene. Prevalence rates were evaluated in relation to various risk factors, including region, housing, vaccination, deworming, pregnancy, reproductive problems, number of dogs living together and hygiene conditions. In total, 21 (33.3%) of 63 vaginal samples were positive for CHV-1 DNA. The prevalence rate in farms (7/16; 43.7%) was higher than in kennels (14/47; 29.7%). No association was found between CHV-1 prevalence and potential risk factors. CHV-1 is highly prevalent in dogs in Iranian farms and kennels. Since the CHV1 vaccine is unlicensed in Iran, effective management strategies are essential to reduce the consequences of this pathogen.

Genomic Diversity and Runs of Homozygosity in Bernese Mountain Dogs

Bernese mountain dogs are a large dog breed formed in the early 1900s in Switzerland. While originally farm dogs that were used for pulling carts, guarding, and driving cattle, today they are considered multi-purpose companion and family dogs. The breed is predisposed to several complex diseases, such as histiocytic sarcoma, degenerative myelopathy, or hip dysplasia. Using whole-genome sequencing (WGS) data, we assessed the genomic architecture of 33 unrelated dogs from four countries: France, Sweden, Switzerland, and the United States. Analysis of runs of homozygosity (ROH) identified 12,643 ROH with an average length of 2.29 Mb and an average inbreeding coefficient of 0.395. Multidimensional scaling analysis of the genetic relatedness revealed limited clustering of European versus USA dogs, suggesting exchanges of breeding stock between continents. Furthermore, only two mtDNA haplotypes were detected in the 33 studied dogs, both of which are widespread throughout multiple dog breeds. WGS-based ROH analyses revealed several fixed or nearly fixed regions harboring discreet morphological trait-associated as well as disease-associated genetic variants. Several genes involved in the regulation of immune cells were found in the ROH shared by all dogs, which is notable in the context of the breed's strong predisposition to hematopoietic cancers. High levels of inbreeding and relatedness, strongly exaggerated in the last 30 years, have likely led to the high prevalence of specific genetic disorders in this breed.

Prevalence of Echinococcus granulosus (Cestoda: Taeniidae) Infection in Stray Dogs and Local Herbivores in Al-Kharj, Saudi Arabia

Background: Echinococcus granulosus is a cyclophyllid cestode parasite that lives as an adult in the intestine of canids and is the most common cause of hydatidosis in ruminants (intermediate hosts). It also offers a public health risk because of the possibility of human infection. The incorrect disposal of dead animals, access of farm dogs to the internal organs of sheep and grazing in areas with wayward dogs are the most common production methods that might increase the risk of sheep being exposed to hydatidosis. The purpose of this study was to investigate the natural spread of E. granulosus infection in wayward dogs, as well as the sterility and fertility rates of hydatid cysts in herbivores of Al-Kharj (Saudi Arabia). Methods: Fecal samples from 150 dogs were collected from several locations in Al-Kharj (Saudi Arabia) where livestock rearing takes place and the existence of wayward and semi-feral canines from August 2021 to February 2022. Parasitic eggs were recovered from fecal samples using the flotation method, followed by centrifugation at 1200 × g. Examination of slaughtered animals was done by comprehensive visual examination, palpation and incision of the internal organs, including lungs, liver, muscles, kidneys and heart. All hydatid cysts detected in the organs were collected for cyst count, cyst fertility testing and protoscolices viability testing. Examination of cysts and viability of protoscolices. A 0.1% aqueous solution of eosin stain was used to examine the survival of protoscolices. According to Smyth and Barret (1980), deceased protoscolices became stained (purplish-red color) five minutes after exposure to staining, whereas living protoscolices remained colorless (recorded as alive) under the microscope. Result: 1698 sheep, 450 goats and 240 camels (slaughtered in Al-Kharj Abattoir) were examined as to their livers, lungs, muscles, kidneys and hearts were checked for hydatidosis. In a study of 150 dogs, 21 (14%) were shown to be naturally infected with E. granulosus eggs. Hydatid cysts were also identified in 156 (9.18%) sheep, 36 (7.5%) goats and 24 (10%) camels. In comparison to other tissues, the liver and lungs had the highest percentage of infestation, with values of 50.6% and 25.6% in sheep, 58.3% and 16.6% in goats and 66.6% and 16% in camels, respectively. The older age groups had higher infection rates than the younger ones. The highest percentage was seen in sheep over the age of 12 months (10.4%) and camels over the age of 6 years (12%). Hydatidosis is a serious economic and zoonotic problem. Therefore, we should take preventative measures in Al-Kharj city to avoid environmental pollution and infection of both humans and animals.

Analysis of virulence genes, drug resistance detection, and pathogenicity in Enterococcus from farm animals

This study aimed to investigate the presence of eight virulence genes (ace, asa1, esp, efaA, gelE, cylA, agg, fsr) in Enterococcus from a variety of animals and to explore the drug resistance and pathogenicity. This could provide a theoretical basis for clinical treatment of Enterococcus infections. Anal swabs from pigs, chickens, cattle, and dogs in farms and pet hospitals were collected for Enterococcus isolation and identification. Eight virulence genes were detected (PCR method), and drug resistance was assessed (drug-sensitive paper method). The strains containing different virulence genes were then divided into EV1, EV2, and EV3 groups. The LD50 and pathogenicity was examined by intra-peritoneal injection to infect mice. Differences were found in the detection rates of virulence genes in Enterococcus from the different animals. The highest overall detection rate was for the esp gene (78.0%), and the lowest for the cylA gene (15.5%). Eight genes were detected most frequently in Enterococcus from dogs and least frequently from cattle. Among the Enterococcus strains from four variety of animals, drug resistance was highest against sulfamethoxazole (100%), cefotaxime (>97%), and cefotaxitin (>93%). Drug resistance was lowest against vancomycin (0%), levofloxacin (<12%) and ciprofloxacin (<13%). The LD50 for each of the three groups was EV1LD50=8.71×109CFU， EV2LD50=2.34×1010CFU，and EV3LD50=9.33×1010CFU. The Enterococcus12LD50 dose group caused significant clinical symptoms in mice, with pathological effects on the heart, liver, lungs, and kidneys, and particularly on the urinary system. The abundance of Enterococcus virulence genes, drug resistance, and pathogenicity vary among different animal origins, and the pathology caused by Enterococcus requires effective treatment protocols based on species and regional characteristics.

Cystic Echinococcosis: An Impact Assessment of Prevention Programs in Endemic Developing Countries in Africa, Central Asia, and South America

Background. Cystic echinococcosis (CE), caused by the tapeworm species, Echinococcus granulosus sensu stricto (G1), is one of many primary neglected zoonoses worldwide. Within endemic developing countries, CE has multiple effects on animal and human health and well-being. To address such effects, veterinary and human medical sector collaboration on prevention program delivery is essential. To begin preliminary evaluations of county specific prevention programs, a critically appraised topic (CAT) was conducted. It sought to answer: What impact do CE prevention programs have on human and animal disease prevalence, in populations living in endemic developing countries within Africa, Central Asia, and South America? Methodology. The aim was to assess the ability of prevention and control program outputs to produce measurable differences in health, social, and economic outcomes (e.g., improved access to medical services, positive behavioral change, or reduced treatment costs, respectively). Included articles were obtained using predefined inclusion/exclusion criteria from the four databases (CAB Abstracts and Global Health; the National Library of Medicine (PubMed); ScienceDirect; and WHO Institutional Repository of Information Sharing (IRIS)). The articles were appraised using three checklists: the Royal College of Veterinary Surgeons (RCVS), the Critical Appraisals Skills Programme (CASP), and the Joanna Briggs Institute checklists. Results. Ten articles were selected. Geographically, 20% of studies were conducted in South America, 30% in Africa, and 50% in Central Asia. For definitive hosts, dogs, CoproELISA antigen testing, before and after Praziquantel (PZQ) de-worming, was a primary focus. For humans, who are intermediate hosts (IH), disease surveillance methods, namely ultrasound (US), were commonly assessed. Whilst for sheep, also acting as IH, disease prevention methods, such as the EG95 livestock vaccine and de-worming farm dogs, were evaluated. Common to all studies were issues of program sustainability, in terms of regular human US screening, dog de-worming, and annual sheep vaccination. This was attributed to transient and remote human or animal populations; limited access to adequate roads or hospitals; few skilled health workers or veterinarians; an over-reliance on communities to administer preventatives; and limited resources. Conclusion. Despite variations in result validity and collection periods, useful comparisons of CE endemic countries produced key research and program recommendations. Future research recommendations included testing the significance of multiple program outcomes in relation to prevalence (e.g., the social outcome: behavioral change), further research on the impact of livestock vaccinations, and the CE transmission role of waterways and sanitation. Program recommendations included calculating and distinguishing between stray versus owned dog populations; formal representation of internal and external stakeholder interests through institutional organization; establishing sustainable guidelines around the frequency of PZQ and vaccination administration; improved veterinary-human medical training and resource sharing; and combined prevention methods and multiple canine disease management.

Detection of Anti-Neospora caninum Antibodies on Dairy Cattle Farms in Southern Italy.

Neosporosis is recognized as one of the major causes of bovine abortion worldwide. Canids are the main definitive host for this parasite and the presence of dogs in the farm is an important factor for the Neospora caninum infection in bovines. Since, in the province of Lecce, located in the Apulia region of Southern Italy, there are no studies showing the presence of the infection in farm animals, the objective was to perform a serological evaluation for anti-N. caninum antibodiesin serum from 706 dairy cattle and 21 farm dogs located in 40 farms uniformlydistributed over the territory.The presence of N. caninum infection was confirmed in 90.0% (36/40) of the 40 farms examined. The results obtained on all serum samples by an enzyme-linked immunosorbent assay (ID Screen® Neospora caninum competition ELISA kit) for anti-N. caninum antibodies showed a seropositivity rate of 21.1% (149/706) among dairy cows, with a statistically significant higher percentage of positive subjects in the animals over two years old and a positivity rate of 42.9% (9/21) in tested dogs. The obtained data confirmed the presence of neosporosis even in the Lecce area, where it could therefore represent an important cause of abortion and economic losses.

Less is more? Ultra-low carbohydrate diet and working dogs' performance.

New Zealand farm working dogs are supreme athletes that are crucial to agriculture in the region. The effects that low or high dietary carbohydrate (CHO) content might have on their interstitial glucose (IG) and activity during work are unknown. The goals of the study were to determine if the concentration of IG and delta-g (a measurement of activity) will be lower in dogs fed an ultra-low CHO high fat diet in comparison to dogs fed a high CHO low fat diet, and to determine if low concentrations of IG are followed by reduced physical activity. We hypothesized that feeding working farm dogs an ultra-low CHO diet would reduce their IG concentrations which in turn would reduce physical activity during work. We prospectively recruited 22 farm dogs from four farms. At each farm, dogs were randomized to one of two diets and had a month of dietary acclimation to their allocated diet. The macronutrient proportions as a percentage of metabolizable energy (%ME) for the high CHO low fat diet (Diet 1) were 23% protein, 25% fat, and 52% CHO, and for the ultra-low CHO high fat diet (Diet 2) 37% protein, 63% fat, and 1% CHO. Following the acclimation period, we continuously monitored IG concentrations with flash glucose monitoring devices, and delta-g using triaxial accelerometers for 96 h. Dogs fed Diet 2 had a lower area under the curve (±SE) for IG (AUC Diet 2 = 497 ± 4 mmol/L/96h, AUC Diet 1 = 590 ± 3 mmol/L/96h; P = 0.002) but a higher area under the curve (±SE) for delta-g (AUC Diet 2 = 104,122 ± 6,045 delta-g/96h, AUC Diet 1 = 80,904 ± 4,950 delta-g/96h; P< 0.001). Interstitial glucose concentrations increased as the activity level increased (P < 0.001) and were lower for Diet 2 within each activity level (P < 0.001). The overall incidence of low IG readings (< 3.5 mmol/L) was 119/3810 (3.12%), of which 110 (92.4%) readings occurred in the Diet 2 group (P = 0.001). In the Diet 2 group, 99/110 (90%) of the low IG events occurred during the resting period (19:00-06:00). We conclude that feeding Diet 2 (ultra-low CHO high fat diet) to working farm dogs was associated with increased delta-g despite decreased IG concentrations. Interstitial glucose concentrations were positively associated with dogs' activity levels independent of diet. Lastly, events of low IG occurred at a low incidence and were predominantly seen between 19:00-06:00 in dogs fed the ultra-low CHO high fat diet.

Seroprevalence and risk factors of Neospora caninum and Bovine Viral Diarrhoea Virus in smallholder dairy cattle in Kenya

Little is known of the risk factors associated with occurrence of Neospora caninum and Bovine Viral Diarrhoea Virus (BVDV) infection in Kenya. This cross-sectional study hypothesized that there are significant biosecurity measures associated with N. caninum and BVDV infections on smallholder dairy farms in Kenya that could be adopted to reduce seroprevalence and impacts. From 158 randomly selected farms in Meru County, Kenya, 470 serum samples were collected from dairy cattle (over six months of age and unvaccinated for these two pathogens). Sera were analyzed for antibodies to N. caninum and antibodies and antigens to BVDV. Data on risk factors were obtained through face-to-face interviews with the farmers. Multivariable logistic regression models were used to identify significant risk factors associated with seropositivity for the pathogens. The apparent seroprevalence of N. caninum, BVDV antibody, BVDV antigen, and co-infection with N. caninum and BVDV antibody and/or antigen were 35.1%, 47.1%, 36.2% and 18.5%, respectively. Risk factors associated with N. caninum antibody included: introducing milking cows into the farm, lending of cattle between farms, farm dogs having access to bovine aborted fetuses, and dogs whelping in the farm compound, with an interaction between the last two variables. BVDV antigen was associated with cattle having contact with pigs, and an interaction between cattle age and whether farms introduced new calves onto farms, and cattle age and whether visiting dairy farmers have access to the cow shed. Cows had higher odds of having BVDV antibodies compared to heifers. Factors associated with co-infection included cow parity, direct contact between dairy cattle, dogs and goats, and introducing new milking cows into the farms. Antibody and antigen results may be partly a function of classical swine fever virus or border disease virus interactions. Farmer education on these biosecurity measures is recommended, along with introduction of BVDV vaccination.

Molecular detection and phylogenetic analysis of Neospora caninum in various hosts from Iran

Different N. caninum strains with distinct biological characteristics and pathogenicity have been introduced from various areas of the world. This study aimed to detect Neospora-infection in different hosts from Iran using the nested-Polymerase Chain Reaction (PCR) technique and to perform the phylogenetic analysis of N. caninum isolates using the Nc-5 gene. Different species of parasite hosts (cattle, dogs, rodents, cats, and birds) from the dairy farms with history of abortion with intensive breeding systems in Hamedan Province, Iran were included for sampling. The stillbirth fetuses of cattle, sheep, and goats were also collected from the slaughterhouse. The nested-PCR technique was used to identify the Neospora-DNA in the aborted fetal materials. The PCR products of the positive samples were sequenced and compared with other isolates in GenBank. Antibodies to N. caninum was found in 19.1% of dogs. Also, 11.4% of dogs were positive for Neospora-like oocysts using microscopy method. The molecular prevalence of N. caninum was 10.6% in dogs, 13.6% in cattle, 35.4% in the aborted fetuses of cows, 31.9% in rodents, 7.6% in pigeons, 1.9% in sparrows, 5.1% in cats, and 7% in the semen sample of bulls. A sampled coyote was positive while the stillbirth fetuses of sheep and goats were negative. The multiple sequence alignments indicated that 34 sequences of N. caninum bore a 94.9-99.3% similarity to each other. Hence, these sequences were classified into two major clusters (clusters A and B) and seven sub-groups (isolates 1-7). The sequence of coyote had a high similarity to the sequences derived from dogs and cows. Also, root diagram showed the isolates of current study and previous reported from Iran have a similar origin. This study provided a phylogenetic analysis of Neospora-infection in different hosts, and for the first time, explored Neospora-infection in coyotes from Iran. Different isolates were detected in the studied population in the region. Comparison of the sequences showed the farm dogs play an important role in the life cycle and distribution of the parasite; so the relationship of dogs with the herd and their access to aborted materials should be restricted. Also, regular control of rodents' population and culling of seropositive cows with a history of abortion is essential for cutting the chain of transmission.

If they could choose: How would dogs spend their days? Activity patterns in four populations of domestic dogs

Although free-roaming domestic dogs (FRDD) constitute the majority of the dog population worldwide, many aspects of their ecology across habitats are little known. Activity budgets by these dogs may also inform management decisions for domestic dogs in human hands. Here we collected data on the activity patterns of owned FRDD from Guatemala (n = 58) and Indonesia (n = 37), and of farm dogs (n = 11) and family dogs (n = 20) in Switzerland. The FRDD from the two countries and the Swiss farm dogs shared the similarity that although they had owners, they spent most or all of the day outside without confinement. Conversely, activity in family dogs is largely controlled by their owners. This cross-continental study thus allowed us to tease apart environmental effects on dogs’ activity from effects due to different levels of control by humans. Dogs were collared with FitBark activity trackers, which measure 3D acceleration, for 2.4–7 days. Activity for each dog was defined as the sum of BarkPoints (a continuous activity metric recorded by the FitBark tracker), calculated for each hour in the 24-hour cycle. The proportion of time resting, in ‘moderate’ and ‘high’ activity (defined by fixed thresholds of BarkPoints) over 24 h was calculated for each dog. The activity patterns of all dogs that (partly) roam freely, i.e. owned FRDD in Guatemala and Indonesia and Swiss farm dogs, showed two peaks over 24 h during 5:00–7:00 h and, less pronounced, 16:00–19:00 h. Such a bimodal activity pattern, which is also observed in other canine species, could only be detected in 45% of the family dogs. Their activity is more dependent on the owners’ daily routines and predominantly showed one high mid-day peak that often changes from day to day. Swiss dogs spent significantly more time resting and less time with ‘moderate’ activity than the owned FRDD. However, family dogs were significantly more often highly active than all other dog groups and compensated with longer resting periods. Activity decreased significantly with age, neutering and increased body condition score, whereas sex did not have any significant influence on activity. Within this study, similarities, but also differences of the activity pattern between owned FRDD and pet dogs could be revealed. Although overall activity levels of the pet dog sample fall in the range of those observed in the less controlled FRDD, it would be of interest to investigate the potential benefit of a more structured daily schedule on pet dogs in future studies.

Evidence for the Continued Occurrence of Chorioretinopathy in Working Sheep Dogs in New Zealand in 2010

Simple SummaryPrevious research in New Zealand on working sheep dogs has found that 39% suffered from retinal eye disease. One of the causes of this eye disease is parasite migration to the eye. Since that research was published in 1987, farmers have been educated to regularly treat farm dogs with anti-parasitic tablets. However, there has been no follow-up studies to see if eye disease is still a problem in working farm dogs. Our study investigated whether eye disease is still present in working sheep dogs in South-West, Waikato, New Zealand. Our study of 184 working sheep dogs and 51 owners was undertaken in 2010, with owners sampled from New Zealand’s South-West Waikato and Tux North Island Dog Trial Championship. Eye examinations were performed on all of the dogs that participated. This study identified that 24% (44/184) of the dogs in the study showed evidence of retinal eye disease. Male working sheep dogs are more susceptible to develop retinal eye disease than females for unidentified reasons. This study concluded that retinal eye disease is still present in working sheep dogs in New Zealand.A study in conducted 1987 by Hughes et al., found that 39% of working sheep dogs had multifocal retinitis. One of the identified causes was ocular larval migrans, which were a result of migrating ascarid larvae. Since that paper was published, anthelmintic use in farm dogs has been highly recommended. There has been no follow-up study to determine if fundic lesions are still present. The current study aimed to investigate the prevalence of chorioretinopathy in working sheep dogs in the South-West, Waikato, New Zealand. This was a cross-sectional study of 184 working sheep dogs and 51 owners, undertaken in 2010 with owners sampled from New Zealand’s South-West Waikato and Tux North Island Dog Trial Championship. Two-way tables were used to explore the relationship between variables. Significance of association was assessed using a Chi-squared or Fisher exact test as appropriate, with a p-value of <0.05 considered significant. Overall prevalence of chorioretinopathy in the working sheep dogs was 44/184 (24%). A significantly higher prevalence of chorioretinopathy was shown in dogs with increasing age, from 2 years to >8 years (p = 0.0007) and in males (p < 0.0001). This study concluded that lesions of chorioretinopathy are still present in working sheep dogs in New Zealand.