Background and purpose: Integrating Generative Artificial Intelligence (Gen AI) in higher education, specifically within health sciences, is increasingly recognized for its potential to enhance educational outcomes and efficiency. The American Association of Colleges of Nursing (AACN) mandates the alignment of Family Nurse Practitioner (FNP) programs with its 2021 Essentials, a competency-based educational framework encompassing hundreds of specific standards. This study aims to evaluate a novel use of Gen AI: how effectively can a custom-trained gen AI tool (custom GPT from ChatGPT), align FNP course assessments with the AACN’s New Essentials, thereby potentially reducing faculty workload and improving curriculum accuracy.Methods: Through dialogue and uploading of relevant documents, a custom GPT (called Mapper) was trained from one FNP course to the subcompentencies within the 2021 Essentials. The Mapper was then used to align the assessments from one FNP course. The Mapper’s output was then compared to content expert alignments to assess accuracy.Results: Across all 10 domains of the AACN Essentials, the Mapper aligned with expert analysis with moderate to high accuracy. Initial analysis indicated correct alignment rate from 44% to 93% (average 66%), which improved to 70% (p < .05) upon further refinement of the Mapper tool by content expert. Potential novel alignments (average 26%), and misalignments (average 9%), provided by the Mapper were critically reviewed, leading to adjustments to the content expert’s original alignment, which enhanced the overall precision of the alignment. For example, misalignments were reduced to only 5% (p < .05). In post-analysis, Mapper aligned AACN subcompetencies incorrectly on average 4%, while the lead faculty was incorrect on average 6%.Conclusions: Gen AI has the potential to streamline the complex process of aligning curriculum to national standards. The GPT demonstrated a significant capacity to assist in this task with minimal error rates, but expert oversight remained crucial to ensure accuracy and relevance. This synergy between Gen AI and human expertise points to a promising avenue for enhancing curriculum development and alignment processes in nursing education and other disciplines.