Faloak Bark Research Articles

Testing the aphrodisiac effects of Sterculia quadrifida (faloak) bark powder on male white rats (Rattus norvegicus)

Background: Sterculia quadrifida R.Br has been widely known and, empirically, has been widely used as an herbal ingredient to overcome sexual disorders in men. Objective: This study aims to assess the quality, stability, and aphrodisiac properties of faloak bark solution preparations (from plants R.Br Sterculia quadrifida) found on Timor Island, specifically on male white rats of the Wistar strain. Method: This research is a type of experimental research. The activity test used two variations of the formula (50%, 100%) extract, positive and negative control tests. Data were analysed with one-way ANOVA (p < 0.05). Result: The test results of extract quality and stability, including moisture content, resting angle, flow speed, dissolving time, organoleptic test, pH, specific gravity, clarity, and homogeneity, met the requirements. The analysis results found significant differences in each test group (p < 0.05), and there were substantial differences between the negative control group and the positive control group, formula one and formula two. Conclusion: The preparation of the solution of Sterculia quadrifida R. met the established quality and stability. The results of the aphrodisiac test of the effect of Sterculia quadrifida R.Br on the parameters of introduction and climbing activity showed significant activity compared to the negative control group.

The effect of concentration and storage time of faloak (Sterculia quadrifida R. Br) bark extract on the ability to inhibit Escherichia coli that cause mastitis

Abstract Mastitis is inflammation that affects the udder of dairy cattle. This inflammation is caused by bacterial infection. One of the bacteria that causes mastitis is Escherichia coli. The use of natural ingredients that are safe and can be used for a long time is highly recommended. Faloak (Sterculia quadrifida R. BR) is a native tree from East Nusa Tenggara which has the potential as a natural antibacterial. The purpose of this research is to determine the concentration and shelf life on the ability to inhibit Escherichia coli. Maceration is the extraction method used in this study, and the solvent used is 96% ethanol. The resulting thick extract was stored in the refrigerator at 5°C. Antibacterial testing with the Kirby Bauer method. The resulting data were analysed with Analysis of Variance Factorial 6x2. There were 12 combinations and 4 replications so that there were 48 experimental units. The results showed that the concentration and shelf life influenced the diameter of the inhibition of Escherichia coli bacteria. Fresh faloak bark extract was better than faloak bark extract was stored for six months. Faloak bark extract concentrations of 30%, 40% and 50% showed were not significantly different. There is no interaction between concentration and shelf life. The extract was stored for 6 months still showed the ability to inhibit Escherichia coli, but its effectiveness decreased by 8.65%.

Potensi Aktivitas Antibakteri Kombucha Kulit Batang Faloak (Sterculia quadrifida R.Br.) dari Pulau Timor

Exploration of the potential of alternative ingredients to replace black tea or green tea in making kombucha has begun to be widely carried out. On Timor Island, faloak stem bark is processed into a traditional drink by some locals. For this reason, the use of faloak stem bark as a basic ingredient for kombucha needs to be studied, especially in exploring its antibacterial potential, which is analyzed based on the concentration of sugar used. This study aims to determine the chemical characteristics and antibacterial activity of faloak stem bark kombucha with various concentrations of sugar. The methods involved making faloak stem bark kombucha with varying sugar concentrations of 6, 8, 10, and 12% (w/v), and testing chemical characteristics in the form of pH, total acid, total sugar, and total phenol tests. Meanwhile, the antibacterial activity testing used a good diffusion method against Escherichia coli ATCC 25922 bacteria. After 14 days of fermentation, faloak stem bark kombucha with a 12% sugar concentration showed the best chemical characteristics, with pH, total acid, total sugar, and total phenol values of 2.91, 1.19%, 7.33%, and 420.15 mg GAE/L, respectively. Furthermore, antibacterial activity also showed the highest inhibition (13.02 mm) at a sugar concentration of 12%. Thus, faloak bark kombucha made with a sugar concentration of 12% has the potential to be antibacterial.

Uji Efektivitas Antibakteri Ekstrak Kulit Batang Faloak (Sterculia quadrifida R.br) yang Tumbuh di Desa Bolok Terhadap Pertumbuhan Bakteri Salmonella Typhi

An impropriate use of antibiotics leads to bacterial resistance problems and side effects. To minimize resistance and adverse side effects, the faloak plant (Sterculia quadrifida R.Br.) has the potential to be used as antibacterial agents. This study aimed to determine the antibacterial effectiveness of faloak bark extract (Sterculia qudrifida R.Br.) against the growth of Salmonella typhi bacteria. This study used a static group comparison research method (Static Group Comparison), namely by measurement (observation) carried out after the treatment group received a program or intervention. According to the results of the research and discussion in this study, the following conclusions can be drawn. Extract of the bark of the faloak plant (Sterculia quadrifida R.Br.) with a concentration of 22.5%; 45%; 75%; and 100% has antibacterial effectiveness against the growth of Salmonella typhi bacteria. The stem bark extract of the faloak plant (Sterculia quadrifida R.Br.) is said to be most effective at inhibiting the growth of Salmonella typhi at a concentration of 100%.

In Vitro and In Vivo Anti Hyperglycemic Evaluation of Sterculia quadrifida Bark through The Inhibition of Alpha Glucosidase

Data from the International Diabetes Federation revealed annual increases in the prevalence of diabetes mellitus, which require special attention. Implementation of the ‘back to nature’ trend presents opportunities to overcome this problem. As a medicinal plant, Faloak is widely used by people in Nusa Tenggara Timur, Indonesia. This study aimed to evaluate in vivo and in vitro anti-hyperglycemic activity of the bark of Sterculia quadrifida, known as Faloak. In vivo evaluation used sucrose-induced male mice. Measurements of glucose level employed a glucometer and data were analyzed statistically. Demonstrating anti-hyperglycemic activity of the bark of Faloak decoct, results showed doses 1.67 and 3.3 g/kg BW reduced glucose levels by 62.2% and 56.9%, respectively. In vitro examination of Faloak decoct at various concentrations used alpha glucosidase (sucrose) enzymatic reaction. Results of in vitro examinations demonstrated of Faloak bark decoct inhibited alpha glucosidase (sucrose) activity by 42.09 ± 4.39%. Further testing on humans is needed to confirm these findings.

Faloak (Sterculia quadrifida) Bark Solution as A Feed Additive on The Internal Organs of Broiler Chickens

The aim of this research was to evaluate the effect of using faloak bark solution in drinking water on the percentage of internal organs of broiler chickens. This research used a completely randomized design consisting of 4 treatments and 5 replications, with 5 chickens in each replication. The treatment given was T0 = without adding faloak bark solution to drinking water, T1 = 1 ml faloak bark solution/l drinking water, T2 = 1.5 ml faloak bark solution/l drinking water, T3 = 2 ml faloak bark solution/l drinking water. Research variables included the relative weight of bursa fabricius, spleen, and kidney. The results of statistical analysis showed that administration of faloak bark solution in drinking water had no significant effect [P>0.05] on internal organs [bursa fabricius, thymus, spleen, and kidneys]. Based on the research results, it can be concluded that giving faloak bark solution up to a dose of 2 ml/drinking water has not been able to improve the immune response of chickens and affect the kidney percentage of broiler chickens.

Karakteristik kimia dan potensi antioksidan dari kombucha berbahan kulit batang faloak (Sterculia quadrifida R.Br)

Faloak (Sterculia quadrifida R.Br) stem bark tea is consumed as herbal drink by some people on Timor Island, East Nusa Tenggara. The potential and bioactive content of faloak stem bark can be increased by fermenting it into kombucha, considering that there has been no use of stem bark, especially faloak stem bark, as the main substrate in kombucha fermentation. This study aims to examine the effects of various concentrations of faloak stem bark tea (0,8, 1,2, 1,6, 2% (w/v)) on the chemical characteristics (pH, total acid, total phenol, total sugar) and the antioxidant potential of faloak stem bark kombucha during the fermentation process on Day 0 and Day 14. During 14 days of fermentation, it was found that the concentration of faloak stem bark tea, with the best concentration of 1.6% (w/v), had a significant effect on changes in chemical characteristics and antioxidant activity, namely pH 2.6, total acid 0.62%, total sugar 7.15% total phenol 467.92 mg/L GAE, total flavonoid 4135.14 mg/L QE, and antioxidant activity of 82.21%. Therefore, faloak stem bark tea has the potential to be used as a substrate in the making of kombucha with tea concentration of 1.6% (w/v) fermented for 14 days.

Faloak (Sterculia quadrifida R.Br.) bark harvesting for curing hepatitis in Kupang City: Herbal medicine in the urban environment

Trees in an urban environment play an important role. Studies have shown that the presence of trees can improve human physical and mental health. People in Kupang, Nusa Tenggara Timur Province, Indonesia, harvest Sterculia quadrifida R.Br. (faloak) bark for medicinal decoction for hepatitis and energy booster. Hepatitis cases in Kupang City continue to increase, from 40 cases in 2016 to 147 cases in 2017 and 220 cases in 2018. This study aimed to assess S. quadrifida bark harvesting intensity and its utilization in Kupang City. This study was conducted in five sub-districts in Kupang, namely Alak, Kelapa Lima, Kota Raja, Maulafa, and Oebobo. In each district, 20 faloak trees were selected to be observed. Parameters observed included: diameter, height, trees that were recently debarked and have recovered bark, distance from the road, and the presence of seedlings around the tree. The data were tabulated and analyzed descriptively. There were 98 trees observed because, in Kota Raja, only 18 faloak trees were found. The recently debarked trees in Kota Raja, Alak, and Maulafa were 54.12%, 49%, and 41.05%, respectively. Meanwhile, trees with recovered bark were found mainly in Kelapa Lima (50%), Oebobo (38.95%), and Kota Raja (31%). Most debarked faloak trees were those closest to roads and settlements with a diameter ranging from 10.38 to 89.17 cm. The distance from the road to the trees was 1-203 m. A large number of debarked faloak trees indicates a high demand for faloak bark.

Determination of Total Flavonoid Content and Initial Test of Anticancer Activity of Faloak Leaves and Fruit Extracts (Sterculia urceolata Smith)

This study aimed to determine the total flavonoid content and anticancer activity of Faloak (Sterculiaurceolata Smith) leaves and fruit extracts. Brine Shrimp Lethality Test (BSLT) method determinedthe anticancer activity. Testing the total flavonoid content of n-hexane extract of the Faloak leavesand fruit were 1558.5 and 498.5 ppm, respectively. The total flavonoid content of the ethyl acetateextract of Faloak leaves and fruit were 876.5 and 621.8 ppm, respectively. The ethanol extract of Faloakleaves and fruit’s total flavonoid content was 338.5 and 1688.5 ppm. The anticancer activity test ofthe n-hexane extract of Faloak leaves and fruit showed that each extract’s LC50 value was 31.417and 50.736 ppm, respectively, and the LC50 value ethyl acetate extract of Faloak leaves and fruit were119.68 and 77.59 ppm. The LC50 value of ethanol extract of Faloak leaves and fruit were 43.82 and20.779 ppm, respectively. Both the leaves and fruit extract of Faloak had strong anticancer activity(<1000 ppm). However, the extracts with the strongest anticancer activity were the ethanol extract ofFaloak fruit and the n-hexane extract of Faloak leaves. Meanwhile, the extract with the highest amountof flavonoid content was the ethanol extract of Faloak fruit (1688.5 ppm), and the lowest amount wasethanol extract of the Faloak bark (4.5 ppm).

Antiplasmodial activity of faloak bark (Sterculia quadrifida, R.Br.) extract from East Nusa Tenggara, Indonesia

The current development of antimalarial drug resistance encourages researchers to discover and develop novel antimalarials. One of its alternatives for antimalarial discovery is using medicinal plants remembering the success of artemisinin. Sterculia quardrifida R. Br. bark, locally name as faloak, is an endemic medicinal plant from East Nusa Tenggara that has been used traditionally to treat malaria. However, its antimalarial activity has not been investigated, yet. This study was aimed to evaluate the antiplasmodial activity of ethanolic extract of faloak bark against Plasmodium falciparum in vitro. Using FCR-3 P. falciparum strain, the ethanolic extract was evaluated on various concentration (1, 10, 50, and 100 μg/mL, respectively). The IC50 value was determined by the relationship between concentration and percentage of growth inhibition. The result showed that the percentage of inhibition of P. falciparum was concentration dependent, higher concentration resulting on higher percentage of inhibition with the IC50 42.399 ± 9.517 μg/mL. It can be concluded that the ethanolic extract of faloak bark have moderate antiplasmodial activity against P. falciparum in vitro.

Acute Toxicity Study of Standardized Faloak Bark ( Sterculia quadrifida R. Br.) Extract on Wistar Rats

The bark of Faloak (Sterculia quadrifida R. Br., Malvaceae) is used for traditional medicine in Indonesia. This study aims to examine the level of toxicity of the Faloak bark standardized ethanolic extract. The barks were collected from East Nusa Tenggara, Indonesia. TLC profile was determined by TLC-densitometry, total phenolic and flavonoid content were determined in ethanolic extract from bark of faloak (S.quadrifida) by UV-V is method. The acute toxicity was performed according to the TD420 method by OECD (2001) on Wistar rats. The animals were grouped into control and treatment groups (2000 mg/kg body weight). All animals were evaluated for possible toxicity signs by measuring body weight, food and water consumption, and histopathological observation. The result of mean concentration of total phenolic content was found to be 17,69±2,01 (%GAE) and concentration of total flavonoid content was found to be 8,56 ± 0,02 (%NE). The results showed that no mortality occurs after 14 days of treatment. In general, no significant changes in animal behavior, body weight, food and water consumption compared to the control group. Histological observation found no sign of toxicity on vital organs. LD50 values obtained from the acute toxicity test results for Faloak ethanol extract (Sterculia quadrifida R. Bark) are higher than 2,000 mg/kg BW and categorized as having a low level of toxicity.

Immunomodulatory Activity Test of Syrup Dosage Form of Combination Phyllantus niruri Linn. And Sterculia quadrifida R.Br. Extract

Meniran (Phyllantus niruri Linn.) is used generally as an immunomodulator and hepatoprotector. Empirically, bark of faloak (Sterculiaqudrifida R.Br.) is used as a medicinal plant to cure diseases such as hepatitis, gastroentritis, and diabetes. The combination of meniran extract and bark of faloak extract has the potential as a natural immunomodulatory agents. Immunomodulators are compositions of either recombinant, synthetic, or natural, which can restore the immune system. Therefore it is necessary to optimize the optimal formula in obtaining syrup preparations which have optimal immunomodulatory activity. The aim of this study was to find out the optimal syrup combination of meniran extract and faloak bark extract to produce optimal response values ​​in the in vitro immunomodulatory activity test with TNF-α and NF-κB parameters, and immunomodulatory activity tests in vivo with activity parameters macrophages. The extract was obtained through reflux extraction. The results of the extract were formulated and approved to determine their ability to induce TNF-α and NF-κB, and in the test of mice balb/b (Mus musculus L.) orally for 21 days. The lymph organs were isolated, rinsed with an RPMI-1640 solution, and analyzed using ELISA reader 450 nm. The determination of optimal formulas is done by using Rstudio v.3.5.3 software with ANOVA test parametric test and using Design Expert v.11 software using the Simplex Lattice Design (SLD) method. The results showed that the formula for the preparation of combination syrup to produce results induced TNF-α and NF-κB, and macrophage activity, namely the formula using meniran and faloak 0.96993: 0.0300704.

In vivo Immunomodulatory Activity of Faloak Bark Extract (Sterculia quadrifida R.Br).

Faloak (Sterculia quadrifida R.Br) is widely used as traditional medicine in Indonesia to improve stamina (reduce tiredness for heavy workers). However, no scientific reports so far on the immunomodulatory effect. The aim of this study was to determine the effect of the bark of faloak as immunomodulatory agents by evaluating their effect on BALB/c mice lymphocytes proliferation, the activity of macrophage, nitric oxide production and the immunoglobulin G titer by in vivo techniques. Decoction of the faloak bark was used for the in vivo assay. BALB/c mice were divided into 5 dose groups, each consisting of 5 mice. One group was chosen as the baseline, 3 groups were used for the group treated with the test substance at doses of 7.5, 11.75 and 17.5 g kg-1 of body weight of mice (p.o) and a positive control group was treated with Phyllanthus niruri Linn. (PN) extract (Stimuno®) 0.585 g kg-1 b.wt., (p.o). The test samples were given every day. All mice were induced by hepatitis B vaccine at day 7 and 14. The activity of in vivo assay was determined at day 19. The activity of immunomodulatory effect is expressed in phagocytic capacity, phagocytosis index, nitric oxide, OD of lymphocyte proliferation and IgG titers. The macrophage phagocytic capacity and phagocytosis index were significantly increased (p<0.05), nitric oxide production were altered significantly (p<0.05), but OD of lymphocyte proliferation and production of IgG titers were unchanged (p>0.05). This study showed that the faloak bark could increase the macrophages phagocytic activity, but no effect on lymphocyte cells and therefore did not influence the adaptive immune response.

ANTIOXIDANT ACTIVITIES AND QUALITY TEST OF INSTANT FALOAK (STERCULIA QUADRIFIDA R.BR.) FROM EAST NUSA TENGGARA WITH ADDED GINGER (ZINGIBERA OFFICINALE ROCH)

Introduction: Faloak is one of the medicinal plants which grows wildly in Kupang-East Nusa Tenggara. People use faloak bark to treat several diseases. The faloak bark is usually processed by boiling and, the cooking water is drunk. Scientifically, faloak bark contains flavonoids which are known to have antioxidant properties. Preparation of faloak in other dosage forms is made so that people have other choices in using faloak. Faloak in the form of instant dosage has been made and has been measured for its antioxidant activity but has very weak antioxidant activity with an IC50 value of 2,307.77 ppm ± 58.20 ppm or 2,249.57 ppm to 2,365.97 ppm (Faizal, 2018). Ginger is a plant that is known to have antioxidant activity. Objective: This study aimed to determine the antioxidant strength of instant faloak by adding ginger based on IC50 value and the quality of the preparation. The sample is taken by taking the faloak bark that is not too old. The bark that has been extracted from the juice is then added with sugar and ginger juice and then recrystallized to get instant faloak with a ginger flavor. The instant faloak was identified qualitatively and measured its antioxidant activity against DPPH as a free radical at a wavelength of 517.4 nm using a UV-vis spectrophotometer. The quality of instant faloak preparations is measured including: water content and ash content. Results: The results showed that instant faloak wich added ginger had very weak antioxidant activity with values ​​of IC50 2,044.2 ppm ± 32.84 or 2,011.42 ppm to 2,077.1 ppm, water content of 2.40% and ash content of 1.46%.

In Vitro Immunomodulatory and Cytotoxic Potentials of Faloak (Sterculia quadrifida R.Br.) Bark

Faloak (Sterculia quadrifida R.Br., Sterculiaceae) is an Indonesia indigenous plant widely used as a traditional remedy for various health complaints. This study aimed to explore the immunomodulatory and cytotoxicity potentials of Faloak bark by using in vitro techniques. Barks was extracted using water, ethanol and ethanol: water (1:1) separately. Colorimetric analysis was used for the determination of the total phenolic and the total flavonoid contents. Immunomodulator activities of the extracts were evaluated by studying their effects on mice macrophages and lymphocytes using in vitro methods, while the cytotoxicity potential was evaluated against Vero and HepG2 cell lines. The active extract was further fractionated and tested using different cell lines by the MTT method. Data were analyzed using one-way ANOVA and LSD tests, with a 95% confidence level (p<0.05). All extracts significantly increased the phagocytosis of macrophages compared to that of control in a concentration-dependent manner. However, all the extracts exhibited the Stimulation Index value of < 2, which indicated no effect on lymphocyte proliferation. The ethyl acetate fraction showed the highest activity against T47D, MCF7 and HepG2 cells with IC50 9.56; 7.62; 3.24 µg/mL and the Selectivity Index (against Vero cells) as follows, 2.01; 2.52; 5.94, respectively. Faloak bark extracts can stimulate macrophage phagocytosis activity in vitro, which positively correlates to the contents of flavonoids and other phenolic compounds. The ethyl acetate fraction exhibited the highest activity, which supports its potency as cancer chemopreventive herbal medicine.

Acute Tocixity of Sterculia quadrifida R.Br Bark Ethanol Extract on Sprague-Dawley Rats

Faloak (Sterculia quadrifida R.Br) is a medicinal plant used by people in the province of East Nusa Tenggara to treat hepatitis. This study aims to determine the acute toxicity of faloak (Sterculia quadrifida R. Br) bark ethanol extract in Sprague-Dawley (SD) male white rats. The method used is experimental using post-test only control group design. In this study the parameters used are body weight, clinical symptoms, clinical biochemistry (ALT &amp; AST), histopathology of liver organ and mortality in rats. Twenty male Sprague-Dawley rats were randomly assigned to 5 groups, group 1 as control, group 2 was administered orally faloak bark ethanol extract at dose of 40 mg / kg body weight, group 3 at dose of 200 mg / kg body weight, group 4 at dose of 1000 mg / kg body weight and group 5 at dose of 5000 mg / kg body weight. Results of observation for 24 hours there are no dead rats, so the value of LD50 extract ethanol faloak is &gt; 5,000 mg / kg body weight. Observations were conducted for 14 days to observed delayed occurrence of toxic effects. Oral administration of the faloak bark ethanol extract reduced the level of AST and ALT. The histopathology observation of rats liver cells showed liver cell necrosis at treatment doses of 200 - 5,000 mg / kg body weight.

Correlation Between Total Flavonoid Contents and Macrophage Phagocytosis Activity of Fractions From Faloak (Sterculia quadrifida R.Br.) Barks Ethanolic Extract In Vitro

On Timor island, Nusa Tenggara Timur, faloak barks (Sterculia quadrifida R.Br.) has been used empirically to restore stamina. Faloak bark ethanolic extract proved to have immunomodulatory activity in vitro, which can increase macrophage phagocytosis activity. This research aimed: (i) to determine the immunomodulatory active fraction of faloak bark ethanolic extract, (ii) to determine the total flavonoid contents of faloak extract and fractions, and (iii) to evaluate the correlation of the total flavonoid contents of those extract and fractions with their macrophage phagocytosis activity. The simplisia powder is macerated with 96% ethanol. The extract was dissolved in methanol:water (9:1v/v) was then subsequently partitioned with n-hexane, ethyl acetate, and water to obtain n-hexane fraction, ethyl acetate fraction, water fraction, and insoluble fraction. Faloak extract and fractions at concentration 62,5; 125; 250; 500μg/mL were tested for their effect on the peritoneal macrophage phagocytosis of Balb/c mice in vitro by the latex beads method. Phagocytosis capacity and phagocytosis index were analyzed using one-way anova and post hoc Tukey HSD test with 95% confidence level. The results showed that ethyl acetate fraction had the highest macrophage phagocytosis capacity and the highest total flavonoid content compared to other fractions. The highest macrophage phagocytosis capacity of ethyl acetate fraction at concentration of 250 μg/mL was 51,94±4,67%, this value was significantly different from cell control (7,50±1,29%), negative controls of 0,0625% dimethylsulphoxide (6,25±0,36%), as well as positive control of 200 μg/mL echinaceae extract syrup® (9,97±0,33%). The total flavonoid content of ethyl acetate fraction determined by aluminum chloride method was 4,290±0.029 mg of quercetin equivalent/g fraction. There was a positive and strong correlation between the total flavonoid content of these extract and fractions with their macrophage phagocytosis capacity (Pearson correlation coefficient of 0,781) and showing linear relationship y=4,721x+19,663; R2=0,61.

Korelasi Kajian Fisikokimia Ekstrak Klika Faloak (Sterculia populifolia DC.) Menggunakan Variasi Pelarut Terhadap Penghambatan Bakteri Patogen

Faloak (Sterculia populifolia DC.) is one of species of sterculiaceae found in East Nusa Tenggara which has potential as a medicinal plant mainly as an antimicrobial. This study aims to determine the correlation of physicochemical study of faloak bark extracts with variation of solvents in inhibiting of pathogenic bacteria. The sample was extracted by maceration method with different polarity level of solvents i.e acetone, acetone 70%, water, ethanol 96%, ethanol 70% and ethanol 50%. The results of extraction through maceration indicate the difference of yield recovery from each of the extraction solvents. The highest yield was obtained from 70% ethanol extract, while the lowest yield of acetone extract. The increase of solvent polarity in this study did not give effect to the amount of recovery of yield. It is also seen from the highest total phenolic content obtained from 70% acetone extract while the lowest in aquadest extract. The antibacterial activity of faloak bark extract on Salmonella typhi was tested using agar diffusion method with 1% of extract solution. Both of ethanol 96% and acetone extracts did not show any inhibitory activity. The largest inhibitory activity was demonstrated by 50% ethanol extract. The polarity level of the extract, the level of total phenolic content and the magnitude of rendement did not show correlation of increased inhibitory activity on Salmonella thypi as well.

Cytotoxic effect of 2,3-dihydro-6-hydroxy-2-methylenenaphtho[1,2-b] furan-4,5-dione compound from the bark of faloak (Sterculia quadrifida R.Br) in breast cancer cells T47D

Faloak ( Sterculia quadrifida R.Br) is used empirically by residents of East Nusa Tenggara for drug plant. The information of the active compound contained in the faloak bark yet specifically published. This study aims to determine the active compound contained in the faloak bark that have cytotoxic activity to T47D breast cancer cells. Extraction process using maceration method, isolation process using gradien isolation method, elusidation using combination of information of IR spectra, 1D-NMR, 2D-NMR and LC-MS, and cytotoxic activity test on T47D breast cancer cells using MTT method. The isolation result obtained that isolate of naphthoquinone derivative compound is 2,3-dihydro-6-hydroxy-2-methylenenaphtho [1,2-b] furan-4,5-dione with IC 50 value on T47D breast cancer cell was 9,88 µg/mL and with an index selectivity value was 30,23.

Isolasi, Identifikasi, Karakterisasi, dan Uji Antibiofilm Derivat Asam Galat dari Kulit Batang Sterculia quadrifida R.Br

Faloak (Sterculia quadrifida R.Br) is used empirically by residents of Timor island to treat hepatitis, typhoid, ulcers, and to restore stamina. Information of active compound contained in the bark of faloak specifically unpublished. This study aims to determine the active compound contained in faloak bark that can be used as antibiofilm. The extraction was performed by soxletation method, the isolation was performed by gradien t isolation method, the elusidation was performed by merging information from NMR and LC-MS spectra analysis. The biofilm inhibition activity test was performed by microdilution method formed on flat bottom flexible microplate U-bottom PVC 96 wells with staining using 1% crystal violet. The isolation process obtained 3 gallic acid derivatives, named isolate 1, isolate 2, and isolate 3. Test of inhibition of biofilm formation showed isolate 1 has IC 50 of 46,87 µg/mL, isolate 2 has IC 50 of 45,87 µg/mL, and isolate 3 has IC 50 42,65 µg/mL. The biofilm test results showed that isolates 1-3 had high biofilm inhibition potency.