Facultative Methylotroph Research Articles

Biopriming using microbial consortia and biostimulants as a technique to increase seed quality of Solanum melongena L. by modifications in morphological and metabolic constituents

Brinjal (Solanum melongena L.) is a widely cultivated vegetable in tropical and subtropical regions. High-quality seeds are crucial for successful crop production, necessitating rapid and uniform field emergence. In the new wave of the Green Revolution, organic wastes were replaced by agrochemicals. This study was conducted from January to March 2024 to standardize biopriming techniques for brinjal using various organic inputs and microbial cultures to improve seed quality. Brinjal seeds cv. PLR 2 were treated with organic inputs (Panchagavya, Egg fermented extract, Fish fermented extract and effective microorganism solution) and liquid microbial cultures (Azospirillum and Pink Pigmented Facultative Methylotroph). The bioprimed seeds were assessed for their physiological attributes and anatomical and biochemical changes. Compared with the control, biopriming with 2% Fish fermented extract significantly improved the germination rate (19.4%), germination energy (40.9%), root length (44.4%), shoot length (24.4%) and seedling vigour index (68.1%). This increase was attributed to the nutritional and bioactive compounds in the fish by-products, which promoted root development and nutrient absorption. The biochemical parameters revealed increased ?-amylase activity, protein, and amino acid content in the bioprimed seeds. Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed beneficial root volatile compounds in the roots of treated seeds. An evaluation of the plant growth-promoting activities of the biopriming agents revealed the superiority of fish-fermented extract over other bioagents. Therefore, this method promotes sustainable agriculture and improved crop productivity, providing a scientific basis for biopriming as a viable alternative to conventional seed treatments.

Isolation and Characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian Ulam Leaves

This study aims to isolate and characterise the Pink Pigmented Facultative Methylotrophs (PPFM) bacteria from the leaves of common Malaysian table salad (ulam). The colonies of PPFM bacteria were obtained using selectively modified Pseudomonas agar based on the appearance of the pink pigment colony. The three selected isolates labelled OJ4, OJ154 [Oenanthe javanica (Blume) DC.] and ML8 [from Melicope lunu-ankenda (Gaertn.) T. G. Hartley] were chosen for characterisation. The result showed the PPFM bacteria colonies (log CFU/g) at the leaf surface of C. caudatus are 4.4 ± 0.1, significantly higher than O. javanica, 3.8 ± 0.2, and M. lunu-ankenda, 3.2 ± 0.1. The selected isolates belong to the Gram-negative group, motile with rod shape with size [length (l) × width (w)] in µm unit 4.3 ± 1.1 × 1.6 ± 0.6, 5.4 ± 0.2 × 1.2 ± 0.1, and 3.5 ± 0.7 × 1.0 ± 0.0, respectively. They show positive urease, catalase, and oxidase activities, while none of them can degrade starch, gelatine, or cellulose, as well as glucose fermentation (MR test) and metabolism actions (VP test), producing indole and hydrogen sulphide gaseous. Only isolate OJ154 demonstrates positive casein hydrolysis and nitrate reduction activities, while only isolate ML8 can utilise citrate but not in lipid degradation. Their sequence analysis of the 16S rRNA indicated that OJ4 and ML8 are Methylobacterium radiotolerans with a similarity of 99%, whereas, OJ154 is Methylorubrum salsuginis with a similarity of 99%. To conclude, PPFM bacteria from the leaves of C. caudatus, O. javanica, and M. lunu-ankenda have been isolated and characterised in particular.

Mannitol as a Growth Substrate for Facultative Methylotroph Methylobrevis pamukkalensis PK2.

Biochemistry of carbon assimilation in aerobic methylotrophs growing on reduced C1 compounds has been intensively studied due to the vital role of these microorganisms in nature. The biochemical pathways of carbon assimilation in methylotrophs growing on multi-carbon substrates are insufficiently explored. Here we elucidated the metabolic route of mannitol assimilation in the alphaproteobacterial facultative methylotroph Methylobrevis pamukkalensis PK2. Two key enzymes of mannitol metabolism, mannitol-2-dehydrogenase (MTD) and fructokinase (FruK), were obtained as His-tagged proteins by cloning and expression of mtd and fruK genes in Escherichia coli and characterized. Genomic analysis revealed that further transformation of fructose-6-phosphate proceeds via the Entner-Doudoroff pathway. During growth on mannitol + methanol mixture, the strain PK2 consumed both substrates simultaneously demonstrating independence of C1 and C6 metabolic pathways. Genome screening showed that genes for mannitol utilization enzymes are present in other alphaproteobacterial methylotrophs predominantly capable of living in association with plants. The capability to utilize a variety of carbohydrates (sorbitol, glucose, fructose, arabinose and xylose) suggests a broad adaptability of the strain PK2 to live in environments where availability of carbon substrate dynamically changes.

Exploring drought mitigation options for improvement of physiology, yield and water use efficiency of aerobic rice (Oryza sativa)

An experiment was conducted during summer seasons of 2022 and 2023 in Wetland Farms at Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu to mitigate the ill effects of moisture stress during the flowering stage of rice (Oryza sativa L.) by moisture stress mitigating chemicals. The trial was laid out in split plot design (SPD) with 3 main plots and 4 sub-plots, and replicated thrice. In main plot, irrigation at IW/CPE ratio 1.0 from emergence to physiological maturity (M1); withholding irrigation (drought stress) for 8 days from heading stage (M2); and withholding irrigation (drought stress) for 12 days from heading stage (M3) and in sub-plot drought stress mitigating chemicals, viz. no spray (S1); salicylic acid spray @100 ppm (S2); sodium selenate @20 ppm (S3); and pink pigmented facultative methylotrophs (PPFM) @1% (S4) were tested. Results revealed that irrigation at IW/CPE 1.0 from crop emergence to physiological maturity along with foliar spray of selenium @20 ppm at heading stage had beneficial impact on growth, physiological parameters, yield attributes and yield. Drought stress given for 8 days from heading stage with selenium spray recorded lesser detrimental effect on growth characters, yield attributes and yield when compared to drought stress given for 12 days from heading stage in aerobic rice cultivation. The yield reduction in drought stress for 12 days from heading stage with selenium @20 ppm spray and drought stress for 8 days from heading stage with selenium @20 ppm spray were 23.6 and 16.5% respectively, when compared to irrigation at IW/ CPE ratio 1.0 from crop emergence to physiological maturity with selenium spray @20 ppm. Water use efficiency and water profitability also increased by the application of stress mitigating chemical of sodium @20 ppm. From this study, it is recommended that, in aerobic rice cultivation if there is any drought occurrence during heading stage of crop, the selenium application @20 ppm can withstand drought even up to 8 days from heading stage.

Effect of Arbuscular Mycorrhizal Fungi and Pink Pigmented Facultative Methylotrophs for Mitigating Water Stress in Black Pepper

Effect of Arbuscular Mycorrhizal Fungi and Pink Pigmented Facultative Methylotrophs for Mitigating Water Stress in Black Pepper

Glycine betaine metabolism is enabled in Methylorubrum extorquens PA1 by alterations to dimethylglycine dehydrogenase.

Low nutrient availability is a key characteristic of the phyllosphere (the aerial surface of plants). Phyllospheric bacteria utilize a wide array of carbon sources generated by plant hosts. Glycine betaine (GB) is a plant-derived compound that can be metabolized by certain members of the phyllosphere microbiota. Metabolism of glycine betaine generates formaldehyde, an intermediate of methylotrophic metabolism, leading us to investigate how the ubiquitous plant colonizing bacterium Methylorubrum extorquens PA1 might metabolize GB encountered in its native environment. M. extorquens PA1 cannot utilize GB as a sole carbon source. Through suppressor mutation analysis, we show that M. extorquens PA1 encodes a conserved GB utilization pathway that can be activated by single point mutations conferring GB utilization as a carbon source. We identified the gene cluster encoding the GB catabolic enzymes and found that gene expression was induced in the presence of GB. We show that utilization of GB is conserved among representative Methylobacterium species and generates the one-carbon metabolism intermediate formaldehyde, which M. extorquens utilizes as a source of energy. Our results support a model where suppressor mutations in Mext_3745 or ftsH (Mext_4840) prevent the degradation of the dimethylglycine dehydrogenase subunit DgcB by the membrane integral protease FtsH, conferring the ability to utilize GB by either (i) restoring stable membrane topology of DgcB or (ii) decreasing FtsH protease activity, respectively. Both mutations alleviate the bottleneck at the second step of GB degradation catalyzed by DgcAB.IMPORTANCEOvercoming low nutrient availability is a challenge many bacteria encounter in the environment. Facultative methylotrophs are able to utilize one-carbon and multi-carbon compounds as carbon and energy sources. The utilization of plant-derived glycine betaine (GB) represents a possible source of multi-carbon and one-carbon substrates. The metabolism of glycine betaine produces formaldehyde and glycine, which may be used simultaneously by facultative methylotrophs. However, the genes required for the utilization of GB in the ubiquitous plant-associated bacterium Methylorubrum extorquens have yet to be identified or described. Our work identifies and validates the genes required for glycine betaine metabolism in M. extorquens and shows that it directly intersects with methylotrophic metabolism through the production of formaldehyde.

Choline degradation in Paracoccus denitrificans: identification of sources of formaldehyde.

Paracoccus denitrificans is a facultative methylotroph that can grow on methanol and methylamine as sole sources of carbon and energy. Both are oxidized to formaldehyde and then to formate, so growth on C1 substrates induces the expression of genes encoding enzymes required for the oxidation of formaldehyde and formate. This induction involves a histidine kinase response regulator pair (FlhSR) that is likely triggered by formaldehyde. Catabolism of some complex organic substrates (e.g., choline and L-proline betaine) also generates formaldehyde. Thus, flhS and flhR mutants that fail to induce expression of the formaldehyde catabolic enzymes cannot grow on methanol, methylamine, and choline. Choline is oxidized to glycine via glycine betaine, dimethylglycine, and sarcosine. By exploring flhSR growth phenotypes and the activities of a promoter and enzyme known to be upregulated by formaldehyde, we identify the oxidative demethylations of glycine betaine, dimethylglycine, and sarcosine as sources of formaldehyde. Growth on glycine betaine, dimethylglycine, and sarcosine is accompanied by the production of up to three, two, and one equivalents of formaldehyde, respectively. Genetic evidence implicates two orthologous monooxygenases in the oxidation of glycine betaine. Interestingly, one of these appears to be a bifunctional enzyme that also oxidizes L-proline betaine (stachydrine). We present preliminary evidence to suggest that growth on L-proline betaine induces expression of a formaldehyde dehydrogenase distinct from the enzyme induced during growth on other formaldehyde-generating substrates.IMPORTANCEThe bacterial degradation of one-carbon compounds (methanol and methylamine) and some complex multi-carbon compounds (e.g., choline) generates formaldehyde. Formaldehyde is toxic and must be removed, which can be done by oxidation to formate and then to carbon dioxide. These oxidations provide a source of energy; in some species, the CO2 thus generated can be assimilated into biomass. Using the Gram-negative bacterium Paracoccus denitrificans as the experimental model, we infer that oxidation of choline to glycine generates up to three equivalents of formaldehyde, and we identify the three steps in the catabolic pathway that are responsible. Our work sheds further light on metabolic pathways that are likely important in a variety of environmental contexts.

Bioprospects of pink pigmented facultative methylotrophs (PPFMs)

PurposeThe purpose of this article is to provide information about interactions between pink-pigmented facultative methylotroph (PPFM) organisms and plants, their molecular mechanisms of methylotrophic metabolism, application of PPFMs in agriculture, biotechnology and bioremediation and also to explore lacuna in PPFMs research and direction for future research.Design/methodology/approachResearch findings on PPFM organisms as potent plant growth promoting organisms are discussed in the light of reports published by various workers. Unexplored field of PPFM research are detected and their application as a new group of biofertilizer that also help host plants to overcome draught stress in poorly irrigated crop field is suggested.FindingsPPFMs are used as plant growth promoters for improved crop yield, seed germination capacity, resistance against pathogens and tolerance against drought stress. Anti-oxidant and UV resistant properties of PPFM pigments protect the host plants from strong sunshine. PPFMs have excellent draught ameliorating capacity.Originality/valueTo meet the ever increasing world population, more and more barren, less irrigated land has to be utilized for agriculture and horticulture purpose and use of PPFM group of organisms due to their draught ameliorating properties in addition to their plant growth promoting characters will be extremely useful. PPFMs are also promising candidates for the production of various industrially and medicinally important enzymes and other value-added products. Wider application of this ecofriendly group of bacteria will reduce crop production cost thus improving economy of the farmers and will be a greener alternative of hazardous chemical fertilizers and fungicides.Graphicalabstract:

Development of Pink Pigmented Facultative Methylotrophs’ (PPFMs) Consortium Formulation and Its Efficacy on Chilli (Capsicum annuum)

This research aims to investigate the potential effect of Pink Pigmented Facultative Methylotrophs (PPFMs) as foliar application to enhance growth parameters of chiili crop. The laboratory and pot studies were conducted at Department of Agricultural Microbiology, Anand Agricultural University, Anand. The PPFMs were isolated from the phyllosphere of solanaceous crop (Chilli, Brinjal, Potato, Tomato and Tobacco) and five out of thirty isolates were selected based on their methanol consuming and plant growth promoting traits. Consortium of isolates (Methylobacterium populi AAU PPFM C-7, M. radiotolerans AAU PPFM C-17, M. populi AAU PPFM C-19, M. populi AAU PPFM T-2 and M. radiotolerans B-2) were prepared after studying their compatibility. Pot study was laid out in net house using completely randomized design with four replications on Chilli crop. The results of the study showed significant effect of PPFMs consortium on growth parameters of chilli (viz. shoot length, root length, shoot weight, root weight, number of branches per plant) that could be result of the plant growth promoting traits of PPFMs.

Distribution and Potential Ecophysiological Roles of Multiple GroEL Chaperonins in Pink-Pigmented Facultative Methylotrophs

Distribution and Potential Ecophysiological Roles of Multiple GroEL Chaperonins in Pink-Pigmented Facultative Methylotrophs

A widespread methylotroph acyl-homoserine lactone synthase produces a new quorum sensing signal that regulates swarming in Methylobacterium fujisawaense.

Bacteria known as pink-pigmented facultative methylotrophs colonize many diverse environments on earth, play an important role in the carbon cycle, and in some cases promote plant growth. However, little is known about how these organisms interact with each other and their environment. In this work, we identify one of the chemical signals commonly used by these bacteria and discover that this signal controls swarming motility in the pink-pigmented facultative methylotroph Methylobacterium fujisawaense DSM5686. This work provides new molecular details about interactions between these important bacteria and will help scientists predict these interactions and the group behaviors they regulate from genomic sequencing information.

Growth and Essential Oil Quantity of Pot Marigold (Calendula officinalis) in Response to Foliar Application of Moringa Extract and Pink-Pigmented Facultative Methylotrophic Bacteria Methylobacterium populi

Growth and Essential Oil Quantity of Pot Marigold (Calendula officinalis) in Response to Foliar Application of Moringa Extract and Pink-Pigmented Facultative Methylotrophic Bacteria Methylobacterium populi

Determination of Antioxidant Activity, Phenolic Compounds, and Toxicity of Methanolic and Ethanolic Extracts of Pink Pigmented Facultative Methylotrophs (PPFM) Bacteria Pigment

Pink-pigmented facultative methylotrophs bacteria are a plant’s surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract’s lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml.

<i>Ancylobacter crimeensis</i> sp. nov., a New Species of Aerobic Methylotrophic Bacteria Isolated from Oak Phyllosphere

Abstract—A new facultative methylotroph, strain 6x-1T, was isolated from the phyllosphere of oak (Quercus pubescens Willd.) on the medium with methanol. Cells were aerobic, gram-negative, not-spore-forming, non-motile short ovoids rods reproducing by binary fission. Growth optimum was at 25‒29°C and pH 7.0‒7.5; growth was inhibited by 1.5% NaCl. Predominant fatty acids were C18:1ω7c and C19:0cyclo. Predominant phospholipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and diphosphatidylglycerol. Tha major ubiquinone was Q10. The 16S rRNA gene sequence of strain 6x-1T exhibited the highest similarity to those of members of the genus Ancylobacter (97.0‒97.4%). Genome analysis of strain 6x-1T and most closely related Ancylobacter strains revealed that the values of ANI (80.7‒83.5%), dDDH (22.4‒23.3%), AAI (72.0‒78.0%), and POCP (62.0‒69.0%) were below the recommended threshold values for prokaryotic species. Genome size of strain 6x-1T was 4.29 × 106 Mb, and G + C content was 67.3%. According to the results of phylogenetic, phylogenomic, phenotypic, and chemotaxonomic analysis, strain 6x-1T (=VKM В-3256T = ССUG 72401T) represents a new species of genus Ancylobacter, for which the name Ancylobacter crimeensis sp. nov. is proposed.

Draft genome sequence of the highly copper-tolerant Methylobacterium radiotolerans MLP1 isolated from the rhizosphere of grasses adjacent to mine tailings.

We present the genome of a highly copper-tolerant pink-pigmented facultative methylotroph isolated from the rhizosphere of grasses growing close to mine tailings. Based on whole-genome taxonomic analyses, this isolate was named Methylobacterium radiotolerans MLP1. Studies are in progress to infer its genome-based copper resistome.

Evaluating of abiotic stress tolerance-inducing substances on the growth, physiology and yield of rice (Oryza sativa L.)

Moisture stress due to delayed onset of monsoon, drought or frequent dry spell is one of the major abiotic stresses that affect rice production in Tamil Nadu. It reduces the yield of rice up to 85 % based on its severity. The foliar application of abiotic stress tolerance inducing substances may aid in mitigating stress-induced damages and increase the yield of rice plants. Salicylic acid, ZnSO4, gibberellic acid, humic acid, pink pigment facultative methylotrophs (PPFM), etc. are reported to increase antioxidant enzyme activities and resistance to drought stress however, its effects on crops were location specific. Field experiments were conducted at Annamalai University Experimental Farm, Annamalainagar, Cuddalore District in Cauvery Delta Zone of Tamil Nadu during “Kuruvai” and “Nanvarai” (Kharif and Rabi) seasons of 2021 and 2022 to study the effect of foliar application of stress tolerance inducing substances on the performance of transplanted rice under moisture stress condition. The experiment was laid out in randomized block design (RBD) with 12 treatment combinations including control; Recommended dose fertilizer (RDF) with + acute soil drying + water spray/ KNO3 @ 2% and ZnSO4 @ 0.5% two foliar sprays/ salicylic acid @ 200 ppm/ gibberellic acid @ 40 ppm/ humic acid @ 0.2%/pink pigment facultative methylotrophs (PPFM) @ 2% and was replicated thrice. Among the treatments, application of recommended dose of fertilizer (120 kg N, 40 kg P2O5 and 40 kg K2O/ha) along with KNO3 @ 2% and ZnSO4 @ 0.5% at panicle initiation and 15 days after first spray and humic acid @ 0.2% at booting stage and 15 days after first spray (flowering stage) increased the plant height of 41.01%, dry matter production of 84.70%, leaf area index of 94.25%, number of productive tillers/m of 32.43%, number of filled grains/panicle of 39.14%, reduced unfilled grains /panicle of 368.23%, increased grain yield of 87.23% and straw yield of 82.57% and benefit cost ratio of 1.86.

Mitigation of Abiotic and Biotic Stress Using Plant Growth Regulators in Rice

Split plot design experiments were conducted to assess the performance of growth regulating compounds for mitigating moisture stress and the incidence of Brown Plant Hopper (BPH) in rice. The main plot treatments (4) comprised moisture stress free control (M1), moisture stress during panicle initiation stage alone (M2), moisture stress during flowering stage alone (M3), and moisture stress during both panicle initiation and flowering stages (M4). The sub-plot treatments (5) were foliar application of growth regulating compounds including chlormequat chloride at 200 ppm (S1), mepiquat chloride at 200 ppm (S2), brassinolide at 0.1 ppm (S3), pink pigmented facultative methylotrophs (PPFM) at 1% (S4), and no spray control (S5). The reduced plant growth attributes were registered under moisture stress at both panicle initiation and flowering stages. The spraying of 1% PPFM during the flowering or both at panicle initiation and flowering stages led to better performance than the other treatments. Also, spraying 1% PPFM brought down the population of BPH to a considerable level during both years of experiments. This suggests that spraying 1% PPFM in the post-flowering stage helps to mitigate the ill effect the moisture stress and BPH incidence.

Effect of moisture stress and growth regulating compounds on water use and yield of transplanted rice

A Field research was carried out at the Wetland Farm, Department of Farm Management, Tamil Nadu Agricultural University, Coimbatore during the Rabi and Summer seasons of 2017 and 2018, to examine the effects of moisture stress and growth-regulating compounds on nutrient uptake, water use efficiency, water productivity, and yield of transplanted puddled rice. Three replications of split plot designs were used for field experiment. The treatments includes foliar applications of growth-regulating compounds, such as chlormequat chloride at 200 ppm, mepiquat chloride at 200 ppm brassinolide at 0.1 ppm , Pink Pigmented Facultative Methylotrophs (PPFM 1%) and Control (No spray) at different growth stages, such as panicle initiation stage, flowering stage, and both panicle initiation and flowering stage. The moisture stress free control is implemented by irrigating the field with 5 cm of irrigation each day after the disappearance of previously ponded water. Treatment combination of moisture stress at panicle initiation stage along with 0.1 ppm brassinolide had recorded to increased WUE of 7.09, 6.68 kg ha-mm-1 and water productivity of 0.71 and 0.67 kg m-3, respectively during Rabi and summer season. Treatment combination of moisture stress at both panicle initiation and flowering stages along with PPFM registered lower WUE with 6.19 and 5.78 kg ha-mm-1 and lower water productivity with 0.62 and 0.58 kg m-3, during both the seasons. it could be concluded that, foliar application of 0.1 ppm brassinolide for mitigating the moisture stress at panicle initiation stage alone and one per cent PPFM spray when crop suffered moisture stress at flowering phase were effective in mitigating the moisture stress in attaining the maximum crop yields.

Pink Pigmented Facultative Methylotrophs (PPFMs): Potential Bioinoculants for Sustainable Crop Production

Bacteria belonging to the genus Methylobacterium, popularly known as pink pigmented facultative methylotrophic (PPFM) bacteria, are well known for their distinct ability to use single-carbon compounds like methanol, formate and formaldehyde, and also a variety of multi-carbon substrates lacking carbon-carbon bonds. These bacterial groups are ubiquitously distributed, especially in phyllosphere and rhizosphere, and their occurrence have been reported in more than 100 species of plants so far. PPFMs have profound influence on soil fertility, crop growth and yield. The ability for phosphate acquisition, nitrogen fixation, iron chelation and phytohormone production indicate the possibility of developing them as promising biofertilizer candidates. In addition, many of them possess biocontrol activity against several phytopathogens. PPFMs induce several physiological changes in plants, making the plants more resistant to biotic and abiotic stress. They can therefore be promising alternatives to conventional chemical inputs in sustainable agricultural systems.

Effect of foliar spraying with Pink Pigmented Facultative Methylotrophic Bacteria as a Foliar Spraying on the Growth and Productivity of Strawberry.

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