Tubers of 30 commercial potato varieties, grown in two different soils, were inoculated withOospora pustulans and incubated in two different conditions in two successive years. Varietal differences in skin and eye susceptibility were in general agreement with those previously recorded in field experiments. There was no consistent varietal difference in symptom expression but in some cases a superficial or slightly depressed skin neerosis developed in addition to the normal pustules. Over all the varieties, there was a significant correlation between surface and eye infection. Skin infection was little influenced by the temperatures of incubation which fluctuated over the range 33–50 F (0.6–10 C). Eye infection tended to be lower where the maximum temperature was higher. Thickness of the tuber periderm and of the suberised layer, and the content of crude fibres in the periderm were all negatively correlated with susceptibility to skin infection. No obvious relation was found between the structure of the eyes and susceptibility to eye infection. The two types of soil used did not influence any of the periderm characters observed or skin or eye susceptibility to skin spot. In the 9 varieties tested, immature and mature tubers showed no significant difference in mean skin susceptibility or in mean periderm thickness.