Extracts Of Green Algae Research Articles

Unveiling the anti-corrosion Potential of seaweed macroalga Codium tomentosum: A multifaceted investigation into its mechanisms of copper protection

The seaweed macroalga Codium tomentosum (GAE) was extracted by the maceration method, and it was used as an eco-friendly, nontoxic inhibitor of copper in 1 M H2SO4 corrosion media. To evaluate the composition and protective capacity of the GAE, several methods were employed, gravimetric measurements, electrochemical testing include polarization curves and electrochemical impedance spectroscopy, Fourier infrared spectroscopy, UV–visible spectroscopy, and scanning electron microscopy. The findings indicate the green algae extract successfully prevents copper corrosion when immersed in a corrosive medium. The inhibition efficiency demonstrates an increase with rising inhibitor concentration, reaching its peak at 82 % at concentration of 1 g/L. Operating solely through adsorption and inhibiting active sites on the copper surface, the inhibitor has a mixed inhibition characteristic. Temperature sensitivity is observed, resulting in a decrease in efficiency. The hypothesis of physisorption nature and adherence to the Langmuir isotherm type is supported by the alignment of thermodynamic and kinetic research. In addition, the inhibition mechanism of GAE was studied by quantum chemical calculations and molecular Monte Carlo simulation. This work provides a useful insight into the anti–corrosion of copper during industrial pickling or descaling processes.

Colorimetric sensor based on biogenic nanomaterials for high sensitive detection of hydrogen peroxide and multi-metals

Hydrogen peroxide (H2O2) and heavy metals, which are among the wastes of the industrial sector, become a threat to living things and the environment above certain concentrations. Therefore, the detection of both H2O2 and heavy metals with simple, low-cost, and fast analytical methods has gained great importance. The use of nanoparticles in colorimetric sensor technology for the detection of these analytes provides great advantages. In recent years, green synthesis of nanomaterials with products that can be considered biowaste is among the popular topics. In this study, silver/silver chloride nanoparticles (Ag@AgCl NPs) were synthesized using the green synthesis method as an eco-friendly and cheap method, the green algae extract was used as a reducing agent. The characterization of Ag@AgCl nanoparticles and green algae extract was carried out with several techniques such as Transmission Electron Microscopy (TEM), UV–Visible spectrometry (UV–Vis), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction patterns (XRD) methods were used for characterization. According to TEM analysis, the Ag@AgCl NPs typically spherical in form and range in size from 4 to 10 nm, and UV–vis showed the formation of surface plasmon resonance (SPR) of the Ag@AgCl between 400 and 450 nm. In addition, its activity as a colorimetric sensor for hydrogen peroxide (H2O2) and multi-metal detection was evaluated. Interestingly, Ag/AgCl NPs caused different color formations for 3 metals simultaneously in the sensor study for heavy metal detection, and Fe3+, Cu2+, and Cr6+ ions were detected. The R2 values for H2O2, Fe3+, Cu2+, and Cr6+ were 0.9360, 0.9961, 0.9787, and 0.9625 the limit of detection (LOD) was 43.75, 1.69, 3.18, and 5.05 ppb (ng/mL), respectively. It was determined that Ag@AgCl NPs have the potential to be used as a colorimetric sensor for the detection of H2O2 and heavy metals from wastewater.

Suppression of hepatocellular carcinoma by Ulva lactuca ulvan via gut microbiota and metabolite interactions

IntroductionUlva lactuca polysaccharide (ULP) is green algae extract with numerous biological activities, including anticoagulant, anti-inflammatory, and antiviral effects. However, the inhibitory ability of ULP in the development of hepatocellular carcinoma warrants further studies. ObjectivesTo elucidate the anti-tumor mechanism of ULP action and evaluate its regulatory effect on gut microbiota and metabolism in H22 hepatocellular carcinoma tumor-bearing mice. MethodsAn H22 tumor-bearing mouse model was established by subcutaneously injecting H22 hepatoma cells. The gut microbiota composition in cecal feces was assessed and subjected to untargeted metabolomic sequencing. The antitumor activity of ULP was verified further by western blot, RT-qPCR, and reactive oxygen species (ROS) assays. ResultsAdministration of ULP alleviated tumor growth by modulating the compositions of the gut microbial communities (Tenericutes, Agathobacter, Ruminiclostridium, Parabacteroides, Lactobacillus, and Holdemania) and metabolites (docosahexaenoic acid, uric acid, N-Oleoyl Dopamine, and L-Kynurenine). Mechanistically, ULP promoted ROS production by inhibiting the protein levels of JNK, c-JUN, PI3K, Akt, and Bcl-6, thereby delaying the growth of HepG2 cells. ConclusionULP attenuates tumor growth in H22 tumor-bearing mice by modulating gut microbial composition and metabolism. ULP inhibits tumor growth mainly by promoting ROS generation.

Effects of Ethanol Extract of Green Algae (Ulva lactuca L) on Histological Description, COX-1 Expression and COX-2 in Ethanol-Induced Rat’s Gastric

Ethanol is the cause of gastric ulcers by reducing the production of prostaglandins (PG) and is closely related to the enzyme cyclooxygenase. One of the plants that has been studied as a gastroprotector is the green algae Ulva lactuca L. The objectives of this research were to study the effects of Ethanol Extract of Green Algae (Ekstrak Etanol Ganggang Hijau / EEGH) Ulva lactuca L. on the histological pictures of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) expression in ethanol-induced gastric ulcer model rats. EEGH is macerated with 96% ethanol. A total of 36 rats were divided into 6 groups with 6 rats in each group. Group I and II were given food and drink, group III was given ranitidine (15.75mg/kgBW). Groups IV, V and VI were given EEGH at doses of 125, 250 and 500mg/kgBW orally. Treatments are given for 14 days. On day 14 the rats were induced with ethanol (except group I) then fasted for 24 hours and then operated on. Gastric histopathology was observed with Hematoxyclin Eosin (HE) staining to see the ability of protection against the stomach of ethanol-induced rats. Gastric protection mechanism was observed by immunohistochemically COX-1 and COX-2 expressions. The results showed that EEGH at doses of 500mg/kgBW had a protective effect as seen from a decrease in the ulcer index and a reduction in necrosis, edema, and hemorrhage. EEGH Ulva lactuca L. were given at a dose of 500mg/KgBW was able to significantly increase the percentage of COX-1 expression in glandular cells but was not able to significantly reduce COX-2 expression.

Potential extract of green algae (Ulva luctuca) as antimicrobial in mouthwash: literature review

Green algae as one of the marine natural resources which is included in the main seaweed is found in many coastal areas of In-donesia. The bioactive component that acts as an antibacterial agent in green algae type Ulva lactuca is ulvan polysaccharide extract. Ulvan is a cell wall polysaccharide that accounts for 9-36% dry weight of Ulva biomass and is mainly composed of rhamnose sulfate, uronic acid (glucuronic acid and iduronic acid) and xylose. Polysaccharide ulvan extracted from Ulva lactuca can be one of the antimicrobial materials used in mouthwash in dentistry.

Burn wound healing activity of ethanol extract gel of Green Algae (Ulva lactuca L) in mice

Recently, burn injuries have become a major cause of morbidity and mortality in low-middle-income countries. Burn injuries are tissue loss caused by contact with heat sources such as hot water, fire, chemicals, electricity, and radiation. According to the WHO Global Burden Disease, in 2017 an estimated 180.000 people died from burn injuries. Ethanol extract of green algae (EEGA) contains compounds that potentially heal burn injuries. The research was conducted to obtain a gel formulation from EEGA and test its burn wound healing properties in mice ( Mus musculus ). Forty-five mice were divided into five groups: I (negative control), II (treated with gel base), III (positive control, Bioplacenton gel), IV (5% EEGA gel), and V (10% EEGA gel). Wound diameters and description scores were observed every fourth, seventh, and fourteenth day, and the derived data were analyzed in the SPSS program with the one-way analysis of variance (ANOVA) and least significance difference (LSD) test. The results indicate that EEGA can be formulated into gels with physical properties compliant with the requirements of the dosage form. Further, it was found that Groups IV and V showed significant reductions in wound description scores and diameters (p<0.05). In conclusion, gels containing 5% and 10% EEGA possess burn wound healing properties.

Using of blue green algae extract and salicylic acid to mitigate heat stress on roselle (Hibiscus sabdariffa L.) plant under Siwa Oasis conditions

Increasing temperature is a global issue due to the existing climate change problem that reduces agricultural productivity and increases prices. It badly affects the yield, and active constituents of medicinal and aromatic plants, especially in newly reclaimed lands in desert areas. The Hibiscus sabdariffa L. is a remarkable crop known for its calyces to make a refreshing drink. It is in other food industries and has many healing effects. A split-plot field experiment was conducted on Hibiscus sabdariffa L. at Siwa Oasis, Egypt, during the 2018 and 2019 seasons for studying to reduce the harmful effect of heat stress on plants and yield improvement. Spraying with three salicylic acid concentrations was put to main plots as 0, 500, and 1000 mg/l and the number of sprays with Spirulina platensis algae extract was allotted to sub-plots and used as 0, 1 and 2 times in the season. Some quantity and quality parameters were under investigation. The significant highest increases in dry weight per plant, number of fruits per plant, dry yield of sepals per hectare and sepal's anthocyanin content were from the combination among spraying with salicylic acid at the concentration of 500 mg/l and spraying with blue green algae extract twice through the season. This treatment was helpful to decrease the high-temperature injury on growth through the summer months.

Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.

The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique. The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs). The extract of Enteromorpha species was analyzed to identify the presence of significant biochemical composition. The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay. It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml). Phytochemicals such as flavonoids and phenolic compounds were found in the extract. The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml). The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively. The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.

Development of antioxidant-rich edible active films and coatings incorporated with de-oiled ethanolic green algae extract: a candidate for prolonging the shelf life of fresh produce.

The concept of sustainability and the substitution of non-biodegradable packaging using biodegradable packaging has attracted gigantic interest. The objective of the present study was to revalorize the biowaste “de-oiled green algae biomass (DAB)” of Dunaliella tertiolecta using a green approach and the development of biodegradable chitosan (CS)-based edible active biocomposite films and coatings for prolonging the shelf life of fresh produce. Ultrasound-assisted green extraction was conducted using food-grade solvent ethanol for obtaining the bio-actives, namely “crude algae ethanolic extract (CAEE)” from DAB. The edible films (CS/CAEE) and coating solutions were developed by incorporating CAEE with varying concentrations (0 to 28%). The CAEE was subjected to MALDI-TOF-MS, NMR, and other biochemical analyses, and was found to be rich in DPPH antioxidant activity (∼40%). The CS/CAEE films were fabricated using a solvent casting method and characterized by several biochemical and physicochemical (FESEM, TGA, FTIR, XRD, WVP, UTM, and rheological) characterization techniques. The addition of CAEE into the CS matrix reduced the maximum film transparency (∼20%), water vapor permeability (∼60%); improved the crystallinity (∼24%), tensile strength (∼25%), and antioxidant activity (∼27%); and exhibited UV-Vis blocking properties as compared to the control film. Besides, the developed coating solutions and CAEE showed biocompatibility with BHK-21 fibroblast cells and antimicrobial activity against common food pathogens. The developed coating solution was applied on green chilli using a dipping method and stored at ambient temperature (25 ± 2 °C, 50–70 % RH) for 10 days. The shelf life of chillies was extended without altering the quality as compared to uncoated green chillies. Therefore, the formulated coating could be applicable for prolonging the shelf life of fresh produce.

Effect of some growth stimulators on productivity, chemical and biological properties of Coleus amboinicus Lour. Cultivated under desert conditions

During the two successive seasons, 2020 and 2021, this investigation was carried out on Coleus amboinicus to study the effect of spraying some plant stimulators on growth, yield, active constituents, and biological activities. The experiment included six foliar spray treatments (control, moringa extract, blue green algae extract, compost tea, active dry yeast extract, and Azolla extract). The applications were arranged in a randomized complete block design with three replicates. The measured data involved growth and yield parameters (plant height, fresh weight of herb per plant, fresh yield of herb per hectare, dry weight of herb per plant, and dry yield of herb per hectare), chemical parameters (essential oil percent, oil content per plant, oil yield per hectare, oil composition, total phenolic content, total flavonoid content), and biological activities (antioxidant and anticancer). The results represented that spraying C. amboinicus with all stimulators significantly increased growth, yield, essential oil percent, oil content per plant, and oil yield per hectare over control. The significant promising treatment was sprayed with blue green algae extract, which recorded the best traits. Moreover, the results showed that using blue green algae extract revealed the highest total phenolic content in the plant while utilizing compost tea represented the highest total flavonoid content. Consequently, using compost tea enhanced in vitro antioxidant and anticancer activities of C. amboinicus.

Green-synthesized silver nanoparticles with aqueous extract of green algae Chaetomorpha ligustica and its anticancer potential

Abstract Marine green algae are rich in various bioactive components with known anticancer activity. Some anticancer drugs present in green algae are in clinical trials nowadays. Algae-mediated silver nanoparticles (AgNPs) have been of a great interest in cancer treatment due to their unique physico-chemical properties. In this study, we evaluate the anticancer efficiency of marine alga Chaetomorpha ligustica collected from the Arabian Gulf against colon cancer cell lines HT29 and HCT116. The anticancer potential of biosynthesized AgNPs from C. ligustica extract is also reported. Fourier transform infrared (FTIR) spectroscopy and gas chromatography-mass spectrometry analyses were used to identify the phytoconstituents present in algae extract. The synthesized AgNPs were confirmed via UV-Vis spectroscopy, whereas their morphology and stability were recorded by transmission electron microscopy (TEM), zeta potential, and zetasizer. We recorded absorption peak at 420 nm; TEM images showed an average size of 8.8 nm, whereas zeta potential and zetasizer study showed aggregation of nanoparticles. FTIR spectroscopy peaks of C. ligustica AgNPs were a little different from those of the C. ligustica extract. Both extracts showed cytotoxicity against cancer cell lines in a dose-dependent manner, but nanoparticles were found to be more toxic than algae extract. HT29 was found to be more sensitive than HCT116. For the first time, species of C. ligustica have been used and reported for the synthesis of nanoparticles. C. ligustica and its biogenic nanoparticles need to be scaled up for many biomedical applications especially in cancer research.

Biostimulative effect of amino acids and green algae extract on capsaicinoid and other metabolite contents in fruits of Capsicum spp.

BackgroundBiostimulants, such as algae extracts or amino acids, are becoming more common in agriculture because the mentality is to make plants more resistant or tolerant to negative environmental factors, rather than using synthetic chemicals (herbicides, insecticides and fungicides), whose use is decreasing year by year, forcing farmers and companies to develop new environmentally friendly products.ResultsIn a field experiment, green algae and amino acids were tested as biostimulants on three hot pepper cultivars. A large variability was observed between the effects of the two biostimulants on the cultivars. Green algae-treated ‘Somborka’ and ‘Habanero Red Caribbean’ cultivars had 10% and 5% higher dry matter in seeds compared to control treatment. Total sugar content was negatively affected by green algae extract and amino acids in pericarp in cultivars ‘Chili AS-Red’ and ‘Habanero Red Caribbean’. Total organic acids content was positively affected by amino acid treatment in ‘Habanero Red Caribbean’ pericarp and placenta, with an increase of 5.5 g and 2.1 g/100 g DW, respectively. In terms of total phenolics, all three cultivars were positively affected by amino acid treatment, but not in each fruit part. In terms of capsaicinoid content, the greatest effect of the two stimulants was on ‘Somborka’, which varied from four (pericarp, seed) to 16 (placenta) times compared to the control. Amino acid extract decreased ‘Habanero Red Caribbean’ capsaicinoid content in placenta by about 40%.ConclusionAmino acids were found to be superior to algal extract, although the effect of both was mostly cultivar specific. For a wider use of biostimulants, it should first be tested whether they are suitable for the cultivar in order to increase and optimize the results.Graphic abstract

Unravelling the antioxidant and anti-cancerous properties of the chemical constituents present in methanol extract of green algae Chaetomorpha antennina

Background: Chaetomorpha antennina is a green seaweed with numerous pharmacological properties such as antimicrobial, antimalarial, anti-tumour and antioxidant properties. Thus, it is essential to perform preliminary studies to identify the chemical composition of seaweed extracts and explore the phytoconstituent responsible for pharmacological activities. Cancer is the leading cause of death worldwide. The discovery of novel therapeutic mediators for the treatment of cancer is the main task. Since efficient drug delivery is possible through nanotechnology, biosynthesis of silver nanoparticles was done, followed by molecular docking studies to investigate the anti-cancer activity of seaweed extract.Methods: Phytochemical analysis of extracts of Chaetomorpha antennina was performed, followed by biosynthesis of silver nanoparticles using these algal extracts. Characterization of silver nanoparticles was done by Scanning Electron Microscopy and X-ray Diffraction. Agar-well diffusion method and 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay were used to determine the antibacterial potential and antioxidant potential of algal extracts. Further, the chemical composition of the extract was distinguished by Gas chromatography-mass spectrometry.Results: Seaweed extracts possessed numerous phytochemicals and methanol extract showed the best antibacterial and antioxidant activity. Docking studies exposed the occurrence of Carpesterol dehydrate and triazines as an anti-cancer component of Chaetomorpha antennina. Conclusion: It can be concluded that seaweed is enriched with chemical constituents possessing high antioxidant activity and therefore, can serve as a promising antioxidant and anti-cancer agent.

FORMULATION OF SOLAR CREAM EXTRACT ETHANOL GANGGANG GREEN (Ulva lactuca L.) AND IN VITRO TEST OF SPF VALUE (SUN PROTECTING FACTOR)

<p>Excessive ultraviolet radiation or UV rays will make the epidermal tissue in the skin unable to resist the harmful effects. Sunscreen is a cosmetic ingredient that functions as a physical or chemical function as a barrier to the penetration of UV rays into the skin. Plants that can function as sunscreens because they have phenolic compounds, one of which is green algae (<em>Ulva lactuca</em> L).</p><p>This type of research is an experimental study using UV-Vis spectrophotometry to determine the ability of sun cream to inhibit UV radiation to the skin. The purpose of this study was to formulate sunscreen cream from ethanol extract of green algae (<em>Ulva lactuca</em> L.) and measure the best SPF (<em>Sun Protecting Factor</em>) value of various concentrations of green algae (Ulva lactuca L.) ethanol extract sunscreen cream.</p><p>The results of the study the SPF value of formula 1 (cream base) was 1.5077, which means formula 1 has no activity as sunscreen, formula 2 (3% green algae extract) with formula 2 (3% extract extract) with an SPF value of 7,311 which is classified as in the extra protection category, formula 3 (4% extract concentration) has an SPF value of 13,427 which is included in the maximum protection category and formula 4 with an SPF value of 19,282 which belongs to the ultra protection category. The cream formulation with the highest SPF value was formula 4. The higher the extract concentration, the higher the SPF value.</p>

Effect of Sulphur Application and some Foliar Feeding on Productivity and Roots Quality of Sugar Beet

A field experiment was conducted at El-Gemmeiza Agric. Res. Station, Gharbeya Governorate, Egypt during the two successive winter seasons of 2017/2018 and 2018/2019, to study the effect of sulphur ( 0, 40 and 80 kg/fad.) and three foliar spray treatments (mixture of FeSO­4 20%+MnSO43H2O 23%+ZnSO47H2O 23% +B as boric acid 17%) at 1%, blue green algae extract at 1 liter /fad. and yeast extract at 100 ml/liter water on growth , yield and root quality of sugar beet (cv Oskar poly) grown in a clay soil conditions. These treatments were arranged in a split plot in a complete block design with three replications. Sulphuer levels were randomly distributed in the main plots , while foliar spray treatments were randomly arranged in the sub plots. The interaction between sulphur at 80 kg/fad. and spraying with blue green algae extract at 1 liter / fad. gave the highest values of sugar beet shoots, roots and its components. While , the interaction between sulphur at 80 kg/fad. and spraying with the mixture of Fe+Zn+Mn+B at 1 % recorded maximum values of Fe, Zn, Mn and B in leaf tissues and roots. The increases in yield of sugar were about 32.8 and 56.3 % for the interaction between 80 kg S/fad. and spraying with blue green algae, 24.9 and 42.7 % for the interaction between 80 kg S/fad. and spraying with mixture of Fe+Zn+Mn+B over the interaction between 0 S and spraying with yeast extract in the 1st and 2nd seasons, respectively.

Gastroprotective Effect of Green Algae Extract (Ulva lactuca L) on Gastric Rats

Gastric ulcer is a disease of the digestive tract characterized by mucous damage to secretion of gastric acid and pepsin. One of the potential gastroprotector plants is green algae. The purpose of this study was to examine the gastroprotective effect of ethanol extract of green algae (EEGH) Ulva lactuca L on the gastric of ethanol-induced rats. This study used 36 rats, divided into 6 groups. Group I is a normal group. Group II negative control, ethanol 96% induced. Group III positive control, given comparison of ranitidine dose of 15.75 mg/kgBW. Groups IV, V and VI were given multilevel EEGH, namely 125, 250 and 500 mg/kgBW. The treatment was carried out for 14 days, on the 14th day ethanol 96% was induced and then dissected. Research data on gastric ulcer index and protection ratio were analyzed using One Way ANOVA with a confidence level of 95%. The results of the EEGH statistical test dose of 500 mg/kgBW with negative controls showed a significant difference with p value of 0.013 (p<0.05). The EEGH 500 mg/kgBW has the ability as an effective gastroprotector, as seen from the gastric ulcer index value of 0.5 and the protection ratio of 78.94%.Keywords: Ethanol, gastroprotector, protection ratio, ulcer index, Ulva lactuca L.78,94%.

The potency of green algae Chaetomorpha crassa Agardh as antioxidant agent from the coastal of Lhok Bubon, West Aceh

The coastal of Lhok Bubon at West Aceh district, Aceh Province is ideal habitat for macro algae, such as Chaetomorpha crassa. The objectives of this paper is to analyse the antioxidant activity of C. crassa; to determine their antioxidant activities correlation with total phenolic contents with different extract of marine macroalgae. The C. crassa samples were collected from the coastal area of Lhok Bubon, West Aceh District (latitude 4011’51.07”N; longitude 9601’42.33”E) Aceh Province, Indonesia in June 2017. Several assay by laboratory approach including chemical composition, extraction, phytochemical screening, DPPH, CUPRAC, FRAP assays and measurement of total phenolic content (TPC) was used. The result showed that the antioxidant activities of three extract of green algae C.crassa clearly indicated that they possess antioxidant activity in different method including DPPH, CUPRAC and FRAP. The correlation has indicated to correlate positively between the total phenol content and antioxidant activity with three methods of antioxidant activity.

Activity of Green Algae Extracts against Toxoplasma gondii

Toxoplasma gondii is a zoonotic protozoa of economic significance in livestock. Infected livestock meat and products act as a source of T. gondii infection in humans. Current drugs against T. gondii are limited by hypersensitivity and toxicity, and are not effective against the encysted bradyzoite stage of T. gondii. Thus, there is urgent need for safe and effective therapeutic agents against T. gondii. Marine algae possess potent antifungal and antibacterial properties, but there are no reports on its anti-protozoal activity. Therefore, in this study we obtained nhexane and methanol extracts of green algae (Chlorophyceae) and analyzed their content by high performance liquid chromatography coupled with mass spectrometry, as well as tested their in vitro anti-Toxoplasma activities. Compared to the n-hexane extract of Chlorophyceae, the methanol extract contained higher content of flavonoids/ polyphenols, alkaloids (elaeocarpidine and auramine), and artemisic acid. Importantly, the methanol extract had more potent anti-Toxoplasma activity (IC50=4.43 ± 1.26 μg/mL) than the n-hexane extract (IC50=23.32 ± 3.97 μg/ mL), corroborating the higher content of flavonoids, alkaloids, and artemisic acid in methanol extracts than in nhexane extract. The anti-Toxoplasma IC50 values of the methanol and n-hexane extracts were 34-fold and 7-fold lower than their respective cytotoxic IC50 values in human fibroblast cell line. Consistent with our findings, flavonoids, alkaloids and artemisic acid have previously been shown to have potent anti-Toxoplasma activity. Together, our results show that Chlorophyceae contains significant amounts of bioactive compounds with potent anti-Toxoplasma activity.

Efek Ekstrak Etanol Ganggang Hijau (Ulva Lactuca L) Terhadap Berat Badan Dan Konsumsi Pakan Tikus Jantan Yang Diberi Diet Lemak Tinggi

Previous studies showed the potential of melatonin of green algae ( U lva lactuca L ) to reduce the risk of coronary heart disease with the activity of antihiperlipidemia. Obesity and hiperlipidemia were the risk factor of degenerative diseases such as heart disease. This research aims was explore the effect of ethanol extract of green algae against weight, consumption of rat feed test white male rat were given high fat diet . Research was started with extraction green algae with ethanol 96 % to obtained concentrated extract. The extract was tested to wistar rats age of 2 months. Animals test divided into 6 group. Group I was control group, treated with high fat diet of lard 2 ml / 200g BW, of group II given high fat diet and simvastatin, group III, IV and V given high fat diet and green algae extract doses 50 mg/ Kg BW, 100 mg/Kg BW and 200 mg/ Kg BW. Group VI is the control group without diet fat high. Treatment was conducted over 28 days. Measuring normal of his weight every five day for 28 days and in measuring consumption feed with measure weight feed the rest of feed early 20 g. The difference of data weight rats per 5 days and consumption fodder for 28 days counted area under curve ( AUC ) from curves time versus weight. The measurement of triglycerides levels. The result showed the high fat diet with lard 2 ml / 200g BW for 28 days reduce weight and consumption feed mice in a significantly than control without high fat diet. The treatment with extract ethanol dose of 50 , 100 and 200 mg / Kg BW did not reduce weight, feed consumption, triglyceride levelsof mice were given high fat diet.

Anti-inflammatory effects of methanolic extract of green algae Caulerpa mexicana in a murine model of ulcerative colitis

Inflammatory bowel diseases, which include Crohn's disease and ulcerative colitis, are characterized by chronic and relapsed gut inflammation. Caulerpa mexicana is a type of green marine algae that can be found in tropical areas, such as the Brazilian Coastland. These macrophytes exhibit in vitro and in vivo anti-inflammatory properties such as the ability to reduce both cell migration to different sites and edema formation induced by chemical irritants. The aim of this study was to examine the effect of the C. mexicana methanolic extract on the treatment of colitis induced by dextran sodium sulfate. Acute experimental colitis was induced in BALB/c mice by treatment with 3% dextran sodium sulfate orally for 14 days. During this 14-day period, C. mexicana methanolic extract (2mg/kg/day) was given intravenously on alternate days. Treatment with the methanolic extract significantly attenuated body weight loss and severe clinical symptoms. This was associated with a remarkable amelioration of colonic architecture disruption and a significant reduction in pro-inflammatory cytokine production. These results suggest that the anti-inflammatory action of C. mexicana methanolic extract on colorectal sites may be a useful therapeutic approach for inflammatory bowel diseases.