Extraction Of Algae Research Articles

Impact of different food-grade protein extraction methods on the proteomic profile and potential allergenicity of Spirulina (Arthrospira platensis)

Various optimized methods for cell disintegration and protein extraction of algae have been reported, but there is a lack of information regarding the proteomic characterization of the extracted proteins; thus, systematic verification of the protein profiles to find potentially exploitable proteins, while minimizing allergenicity risks, is profitable. The method described in this study was developed to thoroughly investigate the proteome characterization of A. platensis following aqueous protein extraction by high-shear homogenization and pH shift. Liquid chromatography-tandem mass spectrometry was applied to assess the proteome obtained from four protein extraction methods using data-dependent proteome analysis. Overall, 699 proteins were identified; however, each extraction method identified unique proteins. The maximum number of exclusive proteins was observed using a combination of high-shear homogenization and acidic pH. The evaluation of protein abundance revealed a clear distinction between proteins isolated from pH 2 treatment compared to those obtained via neutral or basic pH treatment. The applied extraction methods affected the in silico computed physicochemical properties of the proteins. Using the AllerCatPro database, 12 putative allergenic proteins were detected, the most predominant of which were related to the C-phycocyanin beta subunit (P72508). This study demonstrated that extraction methods affect the detection, physicochemical properties, and potential allergenicity of the obtained proteins. Hence, the extraction methods used to obtain novel proteins must consider these aspects before using them as functional foods.

Extraction, antioxidant activity, and hydrogel formulation of marine Cladophora glomerata

In this study, the extraction of marine algae Cladophora glomerata using acetone, methanol, and ethanol solvents was investigated. The extraction yield was in the range of 3.47–4.20 % with a total phenolic content of 2.21–3.40 mg gallic acid equivalent per gram dried weight sample (mg GAE/g DW), and an antioxidant activity IC50 of 0.15–0.39 mg/mL. High chlorophyll and carotenoid contents were observed in all extracts, ranging from 138.44 to 200.79 μg/g DW, 119.05–308.43 μg/g DW, and 15.11–43.45 mg/g DW for chlorophylls a, b, and total carotenoids, respectively. The ethanol extract was selected for further investigation because it has high extraction efficiency and is already used in pharmaceutical preparations.The gas chromatography-mass spectroscopy (GC–MS) profile of the ethanol extract showed palmitic acid (21.4 %), 2,4-di-tert-butylphenol (14.5 %), and dibutyl phthalate (7.7 %) as major compounds. The extract at concentrations of 7.8125–125 μg/mL did not affect the viability of lung fibroblast cells (MRC-5). However, at the concentrations of 250–1000 μg/mL, the extract significantly decreased cell viability. A topical C. glomerata extract hydrogel was developed. The hydrogel had a homogenous texture; was stable in terms of appearance, pH, and spreadability in a variety of low and high temperatures; light and dark storage conditions, and it retained its antioxidant activity after storage. These findings showed that C. glomerata extract hydrogels could potentially be developed as a skin care product on a manufacturing scale.

Antibacterial Activity of Three Algal Genera against some Pathogenic Bacteria

In the current study, three types of algae namely Tetradesmus nygaardi (MZ801740), Scenedesmus quadricauda (MZ801741) and Coelastrella sp (MZ801742) were extracted by 95% ethanol and hexane against two types of gram positive and two types of gram negative bacteria by wells diffusion methods. Eleven concentrations from the extract of algae (2, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 mg/ml) were utilized. It was noticed that ethanolic extraction was more effective than hexane in Scenedesmus quadricauda than the two other mentioned algal species against all pathogenic bacteria, Acintobacter baumanii (ATCC: 19606), Klebsiella pneumonia (ATCC: 13883) Enterococcus faecalis (ATCC: 29212) and Staphylococcus aureus (ATCC: 14028). In addition to that, extraction of Tetradesmus nygaardi by hexane was more effective than ethanol against all studied pathogenic bacteria. Extract of Coelastrella sp by Ethanol showed weak effect against all pathogenic bacteria compared with the other types of algae. Many chemical compounds which possess antibacterial activities were obtained through analyzing the extraction of algae by gas chromatography–mass spectrometry (GC-MS)

Carvone and its eutectic mixtures as novel, biodegradable, and tunable solvents to extract hydrophobic compounds in substitution for volatile toxic solvents

Finding safe solvents with low viscosities has been in great demand in extraction processes. Herein, R-(-)-carvone, a natural monoterpenoid rich in spearmint, was mixed with naturally occurring fatty acids and terpenes. Most eutectic mixtures presented a wide liquid window in the solid-liquid equilibrium phase diagrams. Carvone mixtures at the ideal eutectic points were characterized for physicochemical properties. Despite varying properties, all the tested solvents were immiscible with water and displayed low viscosity with reasonable biodegradability. Sigma potentials of the mixtures were applied to machine learning algorithms, suggesting carvone mixtures as substitutes for polar protic and dipolar aprotic solvents. Carvone mixtures could be successfully applied to liquid-liquid extraction of a red algae called laver, which is rich in natural hydrophobic and hydrophilic pigments of high value. This study proposes carvone as a new bio-based source of hydrophobic solvents and the eutectic mixtures as biodegradable and tunable solvents to extract hydrophobic compounds.

Use of two green algae Ulva lactuca and Ulva intestinalis as bio-fertilizers

In recent years, development have been witnessed in the market expansion of agricultural inputs and products including substances and materials that improve the functioning of soil, plants and the interactions between soil and plant. This is a very broad category of products and substances that often bring innovative solutions in the field of fertilization and crop protection.The objective of this study is to improve the production of market garden where tomato plants in nurseries from powders of two green algae Ulva lactuca and Ulva intestinalis, harvested as bio-fertilizers in the west coast of Mostaganem province. They have minimal effects on the environment, including soil, subterranean water, River and Estuary ecosystems such as Cheliff River, biodiversity and global warming.The experimental part was carried out following the method of extraction of algae and used as a bio-fertilizer on tomato to its simplicity and accuracy, which is the subject of the study of the germination, growth and the development of young plants.The results of this study showed that algal powder of Ulva lactuca and Ulva intestinalis exhibit a remarkable stimulatory effect on the cultivation of tomato, which aims to improve their production, and evident when compared to controls setup. Keywords: seaweed, Mostaganem, River Ecosystem, eco-agriculture, smart agriculture, bio-fertilizers, tomato.

A tale of two alginates.

All fibrous wound dressings are considered to have the same action and value to the support of wound healing. Although clear distinction has been accepted between cotton gauze and calcium alginates, there is still no formally recognised distinction between calcium alginates and the more rapidly gelling fibre dressings. Scientific and clinical evaluations were used to differentiate two different fibrous wound care products. One is derived from polymer extraction of algae (alginate dressings); the other has been manufactured from a uniquely patented carboxymethylation process that produces 100% carboxymethyl cellulose (CMC)-based dressings. Structural differences between these dressings were evaluated with respect to three important areas of wound care management: optimal wound moisture control; the ability to reduce risk of complication by locking away harmful components (e.g. bacteria); and reducing the overall cost of wound care by promoting more efficient use of nursing time. Clear differentiation was illustrated through both scientific and clinical evaluations. This study supports the potential advantages of using a technically advanced fibrous wound dressing over the traditional fibrous alginate wound care product.

Hydrotreating lipids for aviation biofuels derived from extraction of wet and dry algae

Upgrading is a critical step for producing hydrocarbon biofuels. Recently, upgrading algae lipids had attracted a lot of attention, but the attention to aviation biofuel was not enough. Meanwhile, the research of upgrading wet algae lipids was very limited. The hydrogenation characteristics were investigated for lipids derived from extraction of dry and wet algae. Hydrotreating parameters including temperature (270–350 °C) and catalyst loading (10–30%) were investigated and two-stage hydrogenation was conducted at the optimal reaction condition. Two-stage biofuels resulted in lower heteroatoms including O and N both for dry and wet algae lipids. The elemental contents of N were below 0.007% and the O contents were below 0.3%. The heating values were about 46.2 MJ/kg. Both of dry and wet algae lipids can be hydrotreated to aviation biofuel by the two-stage hydrogenation, even though the wet algae lipids contained more carboxylic acid, sterol, and vitamin E than dry algae lipids, which contains more alcohols and esters. The compound of 5-Methyl-3-phenylcyclopent-2-en-1-one is difficult to be upgraded. The content of aromatics and cyclo-paraffins shows a rising trend and some of the aromatics could be upgraded to cyclo-paraffins in the hydrogenation process. In addition, the yield of high-quality biofuel from dry algae lipids is 73.02%, and that is 76.47% for wet algae lipids, which indicated that the wet route hydrogenation might have an advantage over the dry route. The hydrotreating results of model compound cholesterol indicated that much amounts of aromatics was generated.

Batch and Fixed Bed Biosorption of Copper by Acidified Algae Waste Biomass

The aim of this work is to investigate the biosorption of Cu(II) by an acidified waste product from the alginate extraction of algae in batch system and fixed bed columns. The results showed that t...

Extraction of value-added components from food industry based and agro-forest biowastes by deep eutectic solvents

The scientific community, experts in technology and marketing have been seeking cost-competitive and green solvents with good dissolving capacity for the valorisation of biomass and biowaste. Along with traditional solvents and techniques, deep eutectic solvents (DESs) and their bio-analogues, natural deep eutectic solvents (NADESs) are currently emerging as a new class of promising liquid media. In this review, a comprehensive summary of recent contribution of DESs to the processing and valorisation of various kinds of plant and animal based biomass and biowaste is provided. In the field of food industry based and agro-forest waste valorisation, through treatment of the waste, by-products, and natural materials by DESs, several types of compounds, such as flavonoids and other plant phenolics, phenolic acids, stilbenes, tannins, lignans, and lignin were obtained. Extraction of algae by DESs led to isolation mainly of proteins, carbohydrates, lipids and nucleic acids. Vegetable oils, spent oils, residues and by-products of their processing are a rich source of phenolic compounds such as phenolic acids and phenolic alcohols, secoiridoid derivatives (aglycone and ligstroside), lignans (pino and acetoxypinoresinol), flavones (luteolin and apigein), tocopherols and tocotrienols. Dietary fibre serves as a source of lignin, pectic substances, gums, resistant starch, inulin, as well as non-carbohydrate components, e.g., polyphenols, waxes, saponins, cutin, phytates, resistant proteins. Valorisation of wastes originated from animal processing by DESs means obtaining high-value chemicals including amino acids, proteins, bioactive peptides, collagen peptides, albumin etc. Through the valorisation of the mentioned waste types value-added products with potential applications in the pharmaceutical, food and cosmetic industries are produced. The paper gathered data on the used DESs, treated substances and obtained products, together with treatment conditions and the products yields. The evaluation of the state-of-the-art in the field of biowaste valorisation using DESs and NADESs led to conclusions and indication of future prospects and predicted development in this field.

Characterization and properties of sodium alginate from brown algae used as an ecofriendly superabsorbent

Sodium alginate obtained from the extraction of brown algae is used as the backbone for the synthesis of superabsorbent nanocomposite copolymerization. The first stage of extraction is the demineralization process using 0.1 M HCl solution and then 2% Na2CO3 solution for 2 hours at 60°C. The rendement of sodium alginate obtained was 44.32% with molecular weight of 40680 g/mol with measurement of the intrinsic viscosity. FTIR spectra of sodium alginate showed mannuronic acid functional group at wavenumber 884 cm-1 and the uronic acid at wavenumber 939 cm-1, OH functional group at wavenumber 3200-3400 cm-1, and CH2 stretching at wavenumber 2928 cm-1. The diffraction pattern of isolated sodium alginate has specific 2θ at 13.068 and 21.096, amorphous intensity found specific 2θ at 18.058, and the obtained crystallinity degree of the sodium alginate is equal to 29.292% from the XRD analysis. The morphological analysis by SEM shows fibrils of isolated sodium alginate. The success isolation of sodium alginate from brown algae is supported by DSC which shows the decomposition temperature of pure sodium alginate and isolated alginate have close values, namely 251.12°C for pure sodium alginate and 229.90°C for isolated sodium alginate.

Magnetic Nanoparticles Enhanced DNA Extraction and Detection of Forensic Algae by Multiplex PCR Capillary Electrophoresis

Magnetic Nanoparticles Enhanced DNA Extraction and Detection of Forensic Algae by Multiplex PCR Capillary Electrophoresis

Supercritical fluid extraction of algae enhances levels of biologically active compounds promoting plant growth

The aim of this research was to screen plant growth biostimulant properties of supercritical CO2 macroalgal extracts. To this end secondary metabolites were isolated from the biomass of marine macroalgae from the Baltic Sea (species of Polysiphonia, Ulva and Cladophora). Chemical characteristics of the algal extracts were determined by inductively coupled plasma atomic emission spectroscopy for inorganic constituents and high-performance liquid chromatography and spectrophotometry for organic constituents. Inorganic (macro- and microelements) and organic (plant hormones: auxins and cytokinins; polyphenols) compounds were detected in the extract. Algal extracts were tested primarily on garden cress (Lepidium sativum L.; Brassicaceae) and wheat (Triticum aestivum L.; Poaceae). The extracts enhanced chlorophyll and carotenoid content in plant shoots, as well as root thickness and above-ground biomass. The most effective method of application of the extract was by foliar feed on cress and seed maceration for wheat. Algal extracts obtained by supercritical fluid extraction (SFE) were found to be a novel natural source of compounds, stimulating growth of cultivated plants. However, field trials will be required to show that the extracts can act as plant biopesticides and growth biostimulants.

Characterization of Extracted Lipid Obtained from Subcritical Water Extraction of Wet Algae for Biodiesel Production

Characterization of Extracted Lipid Obtained from Subcritical Water Extraction of Wet Algae for Biodiesel Production

Optimization of microwave-enhanced methanolysis of algal biomass to biodiesel under temperature controlled conditions

The effect of a "controlled temperature" approach was investigated in the microwave-enhanced simultaneous extraction and transesterification of dry algae. Experimental runs were designed using a response surface methodology (RSM). The process parameters such as dry algae to methanol ratio, reaction time, and catalyst concentrations were optimized to evaluate their effects on the fatty acid methyl ester (FAME) yield under the "controlled temperature" conditions. Thermal energy associated with the microwave transesterification process was calculated at various temperature levels using the optimized process parameters. Algal biomass characterization and algal biodiesel analysis were carried out using various analytical instruments such as FTIR, TEM, GC-MS and confocal laser scanning microscopy. Thermogravimetric analysis under both nitrogen and oxygen environments was performed to examine the thermal and oxidative stability of the algal fatty acid methyl esters.

Impact of environmental manipulation for Anopheles pseudopunctipennis Theobald control on aquatic insect communities in southern Mexico

Extraction of filamentous algae from river pools is highly effective for the control of Anophelespseudopunctipennis in southern Mexico. We determined the magnitude of changes to the aquatic insect community following single annual perturbations performed over two years. In 2001, algae were manually removed from all the pools in a 3 km long section of the River Coatán, Mexico, while an adjacent section was left as an untreated control. In 2002, the treatments of both zones were switched and algal extraction was repeated. The abundance of An. pseudopunctipennis larvae + pupae was dramatically reduced by this treatment and remained depressed for two to three months. A total of 11,922 aquatic insects from ten orders, 40 families, and 95 genera were collected in monthly samples taken over five months of each year. Algal extraction did not reduce the overall abundance of aquatic insects in river pools, but a greater abundance and a greater richness of taxa were observed in 2002 compared to the previous year. This was associated with reduced precipitation and river discharge in 2002 compared to 2001. Shannon diversity index values were significantly depressed following algal extraction for a period of three months, in both years, before returning to values similar to those of the control zone. However, differences between years were greater than differences between treatments within a particular year. When insects were classified by functional feeding group (FFG), no significant differences were detected in FFG densities between extraction and control zones over time in either year of the study. Similarly, percent model affinity index values were classified as "not impacted" by the extraction process. Discriminant function analysis identified two orders of insects (Diptera and Odonata), water temperature, dissolved oxygen and conductivity, and river volume (depth, width, and discharge) as being of significant value in defining control and treatment groups in both years. We conclude that habitat manipulation represents an effective and environmentally benign strategy for control of An. pseduopunctipennis. Variation in precipitation and river discharge between years was much more important in determining aquatic insect community composition than variation generated by the filamentous algal extraction treatment.

Diversity, Abundance, and Disturbance Response of Odonata Associated with Breeding Sites of <I>Anopheles pseudopunctipennis</I> (Diptera: Culicidae) in Southern Mexico

Odonate nymphs are important predators of the immature aquatic stages of mosquitoes. Populations of the malaria vector Anopheles pseudopunctipennis Theobald (Diptera: Culicidae) can be efficiently reduced by extraction of filamentous algae from river pools in southern Mexico. Here, we examined the influence of this intervention on the diversity of odonates associated with mosquito breeding pools after annual extractions of algae from river pools in a 3-km section of the Coatan River, over a period of 2 yr. Odonate sampling was performed at monthly intervals in control and treated sections of the river for 4–5 mo after extraction in both years and before extraction in 1 yr. In total, 16 species, 10 genera, and 6 families of odonates were collected. Shannon diversity index values declined significantly during a period of 1 mo in 2001 and >5 mo in 2002. However, the abundance of odonates captured was not affected by algal extraction. In contrast, year-to-year variation in the diversity and abundance of the odonate community was strongly influenced by precipitation and river volume. Despite the importance of algae in river ecology, we conclude that the mosquito control intervention resulted in minimal impact on the odonate community in southern Mexico.

Diversity, Abundance, and Disturbance Response of Odonata Associated with Breeding Sites of Anopheles pseudopunctipennis (Diptera: Culicidae) in Southern Mexico

Odonate nymphs are important predators of the immature aquatic stages of mosquitoes. Populations of the malaria vector Anopheles pseudopunctipennis Theobald (Diptera: Culicidae) can be efficiently reduced by extraction of filamentous algae from river pools in southern Mexico. Here, we examined the influence of this intervention on the diversity of odonates associated with mosquito breeding pools after annual extractions of algae from river pools in a 3-km section of the Coatán River, over a period of 2 yr. Odonate sampling was performed at monthly intervals in control and treated sections of the river for 4–5 mo after extraction in both years and before extraction in 1 yr. In total, 16 species, 10 genera, and 6 families of odonates were collected. Shannon diversity index values declined significantly during a period of 1 mo in 2001 and >5 mo in 2002. However, the abundance of odonates captured was not affected by algal extraction. In contrast, year-to-year variation in the diversity and abundance of the odonate community was strongly influenced by precipitation and river volume. Despite the importance of algae in river ecology, we conclude that the mosquito control intervention resulted in minimal impact on the odonate community in southern Mexico.

Optimization of dynamic headspace extraction of the edible red algae Palmaria palmata and identification of the volatile components.

A new extraction method was applied to the volatile compounds of Palmaria palmata. Dynamic headspace was optimized according to an experimental design, and descriptive sensory analysis and intensity and similarity tests were performed for each extract to assess their respective representativeness. Results showed that extract obtained with crushed algae after a 30 min purge was the most representative. GC-MS analysis was then performed on this extract to identify the volatile components. Seven halogenated compounds, seven aldehydes, two ketones, three alcohols, and four miscellaneous compounds were identified. Among them, halogenated compounds were the most characteristic of red algae, and more particularly, iodoethane and iodopentane, which had yet been found in other seaweeds.

Antioxidant activity in vivo and in vitro of Halimeda incrassata aqueous extracts

The aim of the present paper was to provide the evidences for the antioxidant activity in Halimeda incrassata (Ellis) Lamouroux aqueous extracts obtained after simple water extraction of the fresh algae at room temperature (23°C). Previously in the literature, only antioxidant activity associated to carotenoids fractions of seaweeds has been reported. From different species of seaweeds, Halimeda incrassata aqueous extract exhibited the highest antioxidant activity on the inhibition of TBARS formed during the spontaneous lipid peroxidation of rat brain homogenates with an IC50 of 0.340mg.mL-1. Halimeda incrassata aqueous extract (0.5mg.mL-1), was also capable of decreasing the in vitro generation of hydrogen peroxide by two distinct metabolic pathways involving glutamic and malonic acids. Also, Halimeda incrassata (at doses of 50, 100 and 200mg.Kg-1) showed a neuroprotective effect in vivo on the gerbil model of bilateral carotid occlusion because of decreasing the locomotor and exploratory activity induced by ischemia. In summary, Halimeda incrassata aqueous extracts exhibit antioxidant properties in different in vitro as well as in vivo models which could be explained by the presence of several hydrosoluble compounds. Further studies on this way are necessary to elucidate the precise structure of these compounds. Low toxicity of most seaweeds to humans, but particularly of Halimeda genus may favor its use as functional food.

97/01305 Supercritical fluid extraction of algae

97/01305 Supercritical fluid extraction of algae