Extraction Conditions Of Flavonoids Research Articles

Study on the Antioxidant and Anticancer Activities of Sorbus pohuashanensis (Hance) Hedl Flavonoids In Vitro and Its Screen of Small Molecule Active Components

Firstly, the antioxidant capacity of total flavonoids that was extracted from Sorbus pohuashanensis (Hance) Hedl was determined by ABTS+·radical-scavenging method, and MTT assay was used to mensurate the anti-tumor activity of total flavonoids In Vitro. Subsequently, the extraction conditions of flavonoids were optimized by the response surface methodology, the antioxidant activity was investigated simultaneously. Furthermore, the anti-tumor activity mechanism of flavonoids was simulated by molecular docking software. The antioxidant activity of flavonoids was correlated with the content in the process of optimizing the extraction condition. Moreover, The MTT assay revealed that SPF inhibited the activity of four sorts of cancer cells and positively correlated with the concentration of total flavonoids. And the experimental data demonstrated its anticancer activity was better at high concentrations. Finally, its anti-tumor activity was found that may be related to small molecules from flavonoids bind the apoptotic proteins Caspase-3, Caspase-8, and Caspase-9. The study could provide a theoretical basis for further pharmacodynamic research of Sorbus pohuashanensis (Hance) Hedl flavonoids, and improved a valuable attribute to Sorbus pohuashanensis (Hance) Hedl.

Standardization of conditions for extraction of flavonoids from glycyrrhiza glabra callus cultures

Standardization of conditions for extraction of flavonoids from glycyrrhiza glabra callus cultures

Optimization of Extraction of Flavonoids from Shaddock Peel by Response Surface Methodology

Response Surface Methodology (RSM) was applied to predict optimum conditions for extraction of flavonoids from shaddock peel. A central composite design involving extracting temperature, extracting time and ethanol concentration was used and second-order model for extraction ratio of flavonoid was employed to generate the response surface. The optimized condition was as follows: extracting temperature 70°C, extracting time 127.2 min and ethanol concentration 92.16%. The predicted extraction ratio of flavonoid at condition was 81.4216%. Experimental verification gave the value of 80.35%.

Extraction of Flavonoids from T etrastigma hemsleyanum Diels et Gilg and Their Antioxidant Activity

The Box–Behnken design was used to optimize the extraction conditions of total flavonoids from Tetrastigma hemsleyanum. The effects of four independent variables, including extraction temperature, extraction time, number of extraction and the ratio of liquid to raw material, on flavonoids yield were determined. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed using the appropriate statistical methods. The optimum extraction conditions were at extraction temperature of 80C, extraction time of 2.1 h, number of extraction of 3 and ratio of liquid to raw material of 17.6 mL/g. Under these conditions, the experimental flavonoids yield was 1.23 ± 0.09%, which was in good agreement with the predicted value. The antioxidant activities of the flavonoids, including the scavenging activity of ABTS (2,2′-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt), DPPH (1,1-diphenyl-2-picrylhydrazyl) and ferric reducing antioxidant power assay, were examined. The data obtained from our study clearly demonstrate the antioxidant potency of the flavonoids extracted from the roots of T. hemsleyanum. Practical Applications Many studies have been carried out to find new sources of natural antioxidants because they are environmentally friendly and have no health hazards. In particular, interest has intensified in the flavonoids present in the normal human diet and in many folk medicines still in use. Tetrastigma hemsleyanum is a herbaceous perennial species native to China. In this study, the extraction conditions of flavonoids from the roots of T. hemsleyanum were optimized and studies demonstrated that the flavonoids possessed potent antioxidant activity. Therefore, the flavonoids have the potential to be used in food systems.