Root Extension Research Articles

Networking the desert plant microbiome, bacterial and fungal symbionts structure and assortativity in co-occurrence networks

In nature, microbes do not thrive in seclusion but are involved in complex interactions within- and between-microbial kingdoms. Among these, symbiotic associations with mycorrhizal fungi and nitrogen-fixing bacteria are namely known to improve plant health, while providing resources to benefit other microbial members. Yet, it is not clear how these microbial symbionts interact with each other or how they impact the microbiota network architecture. We used an extensive co-occurrence network analysis, including rhizosphere and roots samples from six plant species in a natural desert in AlUla region (Kingdom of Saudi Arabia) and described how these symbionts were structured within the plant microbiota network. We found that the plant species was a significant driver of its microbiota composition and also of the specificity of its interactions in networks at the microbial taxa level. Despite this specificity, a motif was conserved across all networks, i.e., mycorrhizal fungi highly covaried with other mycorrhizal fungi, especially in plant roots—this pattern is known as assortativity. This structural property might reflect their ecological niche preference or their ability to opportunistically colonize roots of plant species considered non symbiotic e.g., H. salicornicum, an Amaranthaceae. Furthermore, these results are consistent with previous findings regarding the architecture of the gut microbiome network, where a high level of assortativity at the level of bacterial and fungal orders was also identified, suggesting the existence of general rules of microbiome assembly. Otherwise, the bacterial symbionts Rhizobiales and Frankiales covaried with other bacterial and fungal members, and were highly structural to the intra- and inter-kingdom networks. Our extensive co-occurrence network analysis of plant microbiota and study of symbiont assortativity, provided further evidence on the importance of bacterial and fungal symbionts in structuring the global plant microbiota network.

Numerical and Experimental Study on Dummy Blade with Underplatform Damper

To confirm the variation in damping ratio offered by dry friction dampers against structural vibration stress, this study developed a blade vibration response test system for capturing damping characteristic curves through both frequency sweep excitation and damping-freevibration methods. The damping-free vibration method demonstrates high efficiency, allowing for the acquisition of a complete damping ratio characteristic curve in a single experiment. Experimental findings indicate that the two contact surfaces on the triangular prism damper produce distinct damping effects, closely aligning with the predicted damping characteristic curves. The peak damping ratio was found to be independent of the centrifugal load of the damper; dampers with varying contact areas produce approximately similar damping characteristics; and the damping effect shows a positive correlation with the root extension length.

Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings

Inhibition of polyamine homeostasis facilitates root extension by modulating IAA and PIN1 distribution in etiolated salt-stressed sunflower seedlings

Impact of Variable Sampling on Estimates of HIV-1 Reservoir Formation Dates.

Timing of human immunodeficiency virus-1 (HIV-1) reservoir formation is important for informing HIV cure efforts. It is unclear how much of the variability seen in dating reservoir formation is due to sampling and gene-specific differences. We used a Bayesian extension of root to tip regression (bayroot) to reestimate formation date distributions in participants from Swedish and South African cohorts, and assessed the impact of variable timing, frequency, and depth of sampling on these estimates. Significant shifts in formation date distributions were only observed with use of faster-evolving genes, while timing, frequency, and depth of sampling had minor or no significant effect on estimates.

Managing Cracked Teeth with Root Extension: A Prospective Preliminary Study Using Biodentine™ Material.

The authors of this study proposed an innovative approach involving the use of Biodentine™ material as an intraorifice barrier in cracked teeth with root extension to promote internal crack sealing, preventing the possibility of microinfiltration and apical crack propagation. The dental records of 11 patients with 12 posterior cracked teeth with root extension were included with a precise protocol performed by a senior endodontist. The treatment protocol included pulp diagnosis, crack identification using a dental operating microscope (DOM), endodontic treatment, placing a Biodentine™ as an intraorifice barrier, and immediate full-coverage restoration. The effectiveness of the treatment was assessed at two intervals, 6 months, and 1-3 years posttreatment, evaluating clinical, radiographic, and tomographic aspects. The treatment was deemed successful if there were no indications of radiolucency, sinus tracts, edema, or periodontal pockets associated with the crack line. The study observed remarkably positive outcomes during the follow-up period, which spanned from 1 to 3 years. All the cracked teeth (100%) remained asymptomatic, meaning they were free of pain or discomfort. Furthermore, these teeth were in occlusal function. Both radiographic and tomographic assessments revealed the absence of bone loss along the crack line. This outcome signifies that the treatment effectively prevented further deterioration of the surrounding bone. Integrating advanced biomaterials and conservative restorative techniques has paved the way for innovative approaches in dental care. This protocol suggests a proactive step for managing cracked teeth with root extension. It addresses both biological aspects by sealing internal cracks and mechanical aspects by preventing crack progression, thereby improving these teeth' prognosis and long-term survival.

"Ectomycorrhizal exploration type" could be a functional trait explaining the spatial distribution of tree symbiotic fungi as a function of forest humus forms.

In European forests, most tree species form symbioses with ectomycorrhizal (EM) and arbuscular mycorrhizal (AM) fungi. The EM fungi are classified into different morphological types based on the development and structure of their extraradical mycelium. These structures could be root extensions that help trees to acquire nutrients. However, the relationship between these morphological traits and functions involved in soil nutrient foraging is still under debate.We described the composition of mycorrhizal fungal communities under 23 tree species in a wide range of climates and humus forms in Europe and investigated the exploratory types of EM fungi. We assessed the response of this tree extended phenotype to humus forms, as an indicator of the functioning and quality of forest soils. We found a significant relationship between the relative proportion of the two broad categories of EM exploration types (short- or long-distance) and the humus form, showing a greater proportion of long-distance types in the least dynamic soils. As past land-use and host tree species are significant factors structuring fungal communities, we showed this relationship was modulated by host trait (gymnosperms versus angiosperms), soil depth and past land use (farmland or forest).We propose that this potential functional trait of EM fungi be used in future studies to improve predictive models of forest soil functioning and tree adaptation to environmental nutrient conditions.

Better Harvest in the Treated Rice Fields and Rice-Husk Charcoals with the Water

We tried the rice plants to better harvest using the specially-processed water, and charcoal made of rice chaffs in Niigata prefecture for a few years and the results are more than twice harvest. Water must be essential to agriculture including soil and bacteria in it. Therefore, we discuss the pico-size water like an elementary particle fabricated with just higher pressure than 100MPa without any additives, so the plant can easily absorb the water leading to the chlorophyll resulting in more extensive roots. Another point is to emit more far-infrared to help the activated charcoals with the water. The result was more than twice the yield of the control. Furthermore, we report the water reduces radioactive cesium of the rice field in Fukushima, leading to stable elements like barium, lanthanum, and cerium. We discuss the basic idea of nuclear changes regardless of present theory.

Anatomical limitations in adventitious root formation revealed by magnetic resonance imaging, infrared spectroscopy, and histology of rose genotypes with contrasting rooting phenotypes.

Adventitious root (AR) formation is one of the most important developmental processes in vegetative propagation. Although genotypic differences in rose rooting ability are well known, the causal factors are not well understood. The rooting of two contrasting genotypes, 'Herzogin Friederike' and 'Mariatheresia', was compared following a multiscale approach. Using magnetic resonance imaging, we non-invasively monitored the inner structure of stem cuttings during initiation and progression of AR formation for the first time. Spatially resolved Fourier-transform infrared spectroscopy characterized the chemical composition of the tissues involved in AR formation. The results were validated through light microscopy and complemented by immunolabelling. The outcome demonstrated similarity of both genotypes in root primordia formation, which did not result in root protrusion through the shoot cortex in the difficult-to-root genotype 'Mariatheresia'. The biochemical composition of the contrasting genotypes highlighted main differences in cell wall-associated components. Further spectroscopic analysis of 15 contrasting rose genotypes confirmed the biochemical differences between easy- and difficult-to-root groups. Collectively, our data indicate that it is not the lack of root primordia limiting AR formation in these rose genotypes, but the firmness of the outer stem tissue and/or cell wall modifications that pose a mechanical barrier and prevent root extension and protrusion.

Celebrating and extending the literary roots of media ecology: Spiralling forward and upward in our times

Many in recent years have been seeking solace and illumination due to trying circumstances by various means including reading. McLuhan’s broad pursuits in literature and into other written materials played an important part in the origins of media studies. This article reviews various sources to confirm as well as advocate further celebration and extension of these literary roots. Moreover, it highlights a recent translation of Machado de Assis’s classic Brazilian novel Brás Cubas as a gateway to increased use of world literature and other diverse sources. To advance McLuhan’s and other foundational works, those involved with media ecology should consider incorporating different recently available fiction and non-fiction more extensively. Such a widening of references could help broaden perspectives, foster additional insights, and help in spiralling the field forward and upward in our times.

Synergistic use of proximally sensed and time series remotely sensed imagery to map soil sodicity

Soil sodicity can cause crusting in the topsoil which impedes water infiltration, and erosion in the subsoil owing to instability. These can impose constraints to sugarcane growth in the form of reduced plant emergence and root extension. To improve soil capability, a map of topsoil (0–0.15 m) exchangeable sodium percentage (ESP) can be used to determine the amount of gypsum required to ameliorate soil condition. Herein, a digital soil mapping (DSM) approach was used to map top- and subsoil (0.3–0.45 m) ESP. To do this, we first examined prediction agreement of a calibration model between digital data and ESP, first comparing individual use of remotely sensed time series imagery (i.e., Sentinel-2 and Landsat-8) or proximal sensor (i.e., electromagnetic [EM] induction and gamma-ray [γ-ray]) data. To account for the large amount of remote data we combined them using principal component analysis (PCA). We also investigated combining digital data using a backward feature elimination – BFE), along with minimum calibration sample size (n = 150–10) determined. Prediction agreement (Lin's concordance correlation coefficient – LCCC) and accuracy (ratio of performance to deviation – RPD) were assessed using an independent validation dataset (n = 32). To predict topsoil ESP, PCs from Landsat-8 provided excellent (LCCC = 0.90) agreement and very good (RPD = 2.26) accuracy, followed by Sentintel-2 (0.86, 1.75), γ-ray (0.83, 1.84) and EM (0.78, 1.78), with subsoil ESP also excellent (0.90) and very good (2.51) using Landsat-8, however; Sentintel-2 outperformed the others with even better results (0.95, 3.24), followed by EM (0.78, 1.76) and γ-ray (0.73, 1.54). Moreover, slightly better results were achieved when EM, γ-ray, and PCs of Sentinel-2 were combined, with top- and subsoil ESP agreement (0.93 and 0.94, respectively) and accuracy (2.53 and 3.14, respectively) excellent. A minimum of 40 sampling sites were required (1.2 sites ha−1) for the good prediction agreement and accuracy of top- (0.85, 2.13) and subsoil (0.84, 1.90) ESP. Based on the best results, DSM of ESP was made to recommend gypsum application rates to ameliorate sodic soils according to the Six-Easy-Steps Management Guidelines. The northern fields required variable rates (5, 7.5, and 10 t ha−1) of gypsum, whereas the southern fields would not require gypsum.

Growth and physiological response of canola (Brassica napus L.) tointeractive effect of temperature, moisture and nitrogen stressesunder controlled environment

Present atmospheric limits growth of C3 crop plants, which show response to elevated CO2 via reduced photorespiration and enhanced photosynthetic rates thereby increased growth and yield. Global research on plant responses to raising atmospheric CO2 has primarily focused on C3 species, advancing our understanding of processes underlying C3 plant acclimation and response. Therefore extensive research has been conducted to project the potential impacts of global warming on agriculture productivity through laboratory by using crop and global climate models. Canola is one of the world’s most important oilseed crops and the most profitable commodity for Canadian farmers. As a C3 cool seasoncrop, it is more susceptible to heat stress than other C3 and C4 field crops (Wu and Ma, 2018). High temperature significantly changes the rate of plant metabolic processes that ultimately reduces biomass accumulation and grainformation. A field study in Eastern Canada reported that seed yield reduced as much as 40% in 2012 due to severeheat and drought stress over normal years (Ma and Herath, 2016). High or warm temperature has been shown toinduce the floral sterility or pollen abortion and shorten seed filling duration and subsequently reduce inflorescence size in canola plants. Even high temperature could alter the partitioning of photoassimilates to roots and suppress root growth extension or change root system architecture. Late seeded canola plants often produced a smaller root system, due to higher temperature and or drought stress encounters during the crop development. Keeping these facts trial was conducted under controlled condition to know the response of canola to drought stress, elevated CO2 and temperature.

Identification of genetic and environmental factors influencing aerial root traits that support biological nitrogen fixation in sorghum.

Plant breeding and genetics play a major role in the adaptation of plants to meet human needs. The current requirement to make agriculture more sustainable can be partly met by a greater reliance on biological nitrogen fixation by symbiotic diazotrophic microorganisms that provide crop plants with ammonium. Select accessions of the cereal crop sorghum (Sorghum bicolor (L.) Moench) form mucilage-producing aerial roots that harbor nitrogen-fixing bacteria. Breeding programs aimed at developing sorghum varieties that support diazotrophs will benefit from a detailed understanding of the genetic and environmental factors contributing to aerial root formation. A genome-wide association study of the sorghum minicore, a collection of 242 landraces, and 30 accessions from the sorghum association panel was conducted in Florida and Wisconsin and under 2 fertilizer treatments to identify loci associated with the number of nodes with aerial roots and aerial root diameter. Sequence variation in genes encoding transcription factors that control phytohormone signaling and root system architecture showed significant associations with these traits. In addition, the location had a significant effect on the phenotypes. Concurrently, we developed F2 populations from crosses between bioenergy sorghums and a landrace that produced extensive aerial roots to evaluate the mode of inheritance of the loci identified by the genome-wide association study. Furthermore, the mucilage collected from aerial roots contained polysaccharides rich in galactose, arabinose, and fucose, whose composition displayed minimal variation among 10 genotypes and 2 fertilizer treatments. These combined results support the development of sorghums with the ability to acquire nitrogen via biological nitrogen fixation.

A labeling study of dentin formation rates during crown and root growth of porcine mandibular first molars.

We used fluorochrome labeling to study spatiotemporal variation of dentin apposition (DAR) and extension (DER) rates during crown and root formation of mandibular first molars from wild boar and domestic pigs. DAR was reconstructed along the course of dentinal tubules in four zones of the crown and in the upper root area. In all five zones, mean DAR increased during the first 30% to 40% of apposition, reaching highest values (22-23 μm/day) in the upper-lateral crown zone. Lowest values were recorded near the dentin-pulp interface (DPI). Typically, DARs in contemporaneously formed dentin areas were higher in more cuspally compared to more cervically/apically located zones. DER was high (>200 μm/day) in early postnatal crown dentin and then decreased markedly in cervical direction, with lowest values in the cervical crown zone. After this nadir, DER sharply increased in the upper 30% to 40% of the root extension, reaching values equaling (wild boar) or even surpassing (domestic pigs) those recorded in the upper lateral crown. After this peak, DER again decreased. While DAR did not differ markedly between wild boar and domestic pigs, the DER showed marked differences, both regarding maximum values (208.1 μm/day in wild boar, 272.2 μm/day in domestic pigs) and the timing of the root growth spurt, which occurred earlier in the domestic pigs. We consider the more rapid recruitment of secretory odontoblasts in domestic pigs (reflected by higher DER) a side effect of selection for rapid body growth during pig domestication.

Effects of Different Nitrogen Application Rates on Root Growth and Distribution of Fine Root Length across Diameter Classes of Wolfberry (Lycium barbarum L.)

The optimized cultivation process of wolfberry (Lycium barbarum L.) to maintain a consistently high and stable yield relies on the prolonged use of significant amounts of nitrogen fertilizers. However, this practice leads to increased production costs and various issues, such as soil pollution and compaction. To address these concerns, a three-year field trial was conducted involving different nitrogen application rates: N1 (20% nitrogen (N) reduction, 540 kg·hm−2), N2 (medium N, 675 kg·hm−2), and N3 (20% nitrogen increase, 810 kg·hm−2). The results showed that the inter-annual growth and development of wolfberry roots had two rapid growth peaks. In comparison with the N3 treatment, the root morphological characteristics index increased significantly under the N1 and N2 treatments. Among the different diameter classes, the most significant increase in fine root length, with an average diameter between 0.4 and 0.8 mm, occurred under the N1, N2, and N3 treatments, accounting for 50.6%, 50.92%, and 47.72% of the total annual growth of root length increments, respectively. Concerning the distribution of fine roots, the active layer depth extended under the N2 treatment suggesting that medium nitrogen application favored the longitudinal extension of fine roots. Leaf nitrogen content and the chlorophyll meter values (SPAD values) in the upper part of the plant, at the tip of shoots/branches, were the most sensitive indicators to changes in nitrogen application rates. These values increased significantly with higher nitrogen application amounts. Similarly, the contents of total sugar, betaine, and β-carotene increased with increasing nitrogen application rates, while the contents of Lycium barbarum polysaccharides (LBPs) and total flavonoids decreased. Finally, based on a comprehensive principal component evaluation, the rankings for root growth and plant development under various nitrogen application treatments were as follows: N2 (1.891) > N1 (0.002) > N3 (−1.894). The results showed that both the aboveground and belowground growth and development of wolfberry plants were most optimized under the N2 treatment. These findings provide a foundational reference for constructing good root morphology of wolfberry through cultivation practices such as nitrogen fertilizer management.

Leading-Edge Vortex Controller (LEVCON) Influence on the Aerodynamic Characteristics of a Modern Fighter Jet

The purpose of this paper is to assess the influence of a novel type of vortex creation device called the leading-edge vortex controller (LEVCON) on the aerodynamic characteristics of a fighter jet. LEVCON has become a trending term in modern military aircraft in recent years and is a continuation of an existing and widely used aerodynamic solution called the leading-edge root extension (LERX). LEVCON is designed to operate on the same principles as LERX, but its aim is to generate lift-augmenting vortices, i.e., vortex lift, at higher angles of attack than LERX. To demonstrate the methodology, a custom delta wing fighter aircraft is introduced, and details about its aerodynamic configuration are provided. The LEVCON geometry is designed and then incorporated into an existing three-dimensional (3D) model of the aircraft in question. The research is conducted using OpenFOAM 8, a high-fidelity computational fluid dynamics (CFD) open-source software. The computational cases are designed to simulate the aircraft’s flight at stall velocities within a high range of angles of attack. The results are assessed and discussed in terms of aerodynamic characteristics. A conclusion is drawn from the analysis regarding the perceived improvements in fighter jet aerodynamics. The analysis reveals that both lift and critical angle of attack can be manipulated positively. With the addition of LEVCON, the average lift gain in the high angle of attack (α) range is between 8.5% and 10%, while the peak gain reaches 19.4%. The critical angle of attack has also increased by 2°, and a flatter stall characteristic has been achieved.

Correlation between plant cell wall stiffening and root extension arrest phenotype in the combined abiotic stress of Fe and Al.

The plasticity and growth of plant cell walls (CWs) remain poorly understood at the molecular level. In this work, we used atomic force microscopy (AFM) to observe elastic responses of the root transition zone of 4-day-old Arabidopsis thaliana wild-type and almt1-mutant seedlings grown under Fe or Al stresses. Elastic parameters were deduced from force-distance curve measurements using the trimechanic-3PCS framework. The presence of single metal species Fe2+ or Al3+ at 10 µM exerts no noticeable effect on the root growth compared with the control conditions. On the contrary, a mix of both the metal ions produced a strong root-extension arrest concomitant with significant increase of CW stiffness. Raising the concentration of either Fe2+ or Al3+ to 20 µM, no root-extension arrest was observed; nevertheless, an increase in root stiffness occurred. In the presence of both the metal ions at 10 µM, root-extension arrest was not observed in the almt1 mutant, which substantially abolishes the ability to exude malate. Our results indicate that the combination of Fe2+ and Al3+ with exuded malate is crucial for both CW stiffening and root-extension arrest. However, stiffness increase induced by single Fe2+ or Al3+ is not sufficient for arresting root growth in our experimental conditions.

Root extension of generalized power series rings

Let R 1 ⊆ R 2 be commutative rings with unity and let S 1 ⊆ S 2 be torsion-free abelian monoids. We give a characterization of when the extension R 1 [ S 1 ] ⊆ R 2 [ S 2 ] of monoid rings is a root extension. For torsion-free, ≤ -cancellative, strictly ordered abelian monoids ( S 1 , ≤ ) ⊆ ( S 2 , ≤ ) , we also give a characterization of when the extension [ ​ [ R 1 S 1 , ≤ ] ​ ] ⊆ [ ​ [ R 2 S 2 , ≤ ] ​ ] of generalized power series rings is a root extension.

Effects of Salt Tolerance Training on Multidimensional Root Distribution and Root-Shoot Characteristics of Summer Maize under Brackish Water Irrigation.

To investigate the impact of brackish water irrigation on the multidimensional root distribution and root-shoot characteristics of summer maize under different salt-tolerance-training modes, a micro-plot experiment was conducted from June to October in 2022 at the experimental station in Hohai University, China. Freshwater irrigation was used as the control (CK), and different concentrations of brackish water (S0: 0.08 g·L-1, S1: 2.0 g·L-1, S2: 4.0 g·L-1, S3: 6.0 g·L-1) were irrigated at six-leaf stage, ten-leaf stage, and tasseling stage, constituting different salt tolerance training modes, referred to as S0-2-3, S0-3-3, S1-2-3, S1-3-3, S2-2-3, and S2-3-3. The results showed that although their fine root length density (FRLD) increased, the S0-2-3 and S0-3-3 treatments reduced the limit of root extension in the horizontal direction, causing the roots to be mainly distributed near the plants. This resulted in decreased leaf area and biomass accumulation, ultimately leading to significant yield reduction. Additionally, the S2-2-3 and S2-3-3 treatments stimulated the adaptive mechanism of maize roots, resulting in boosted fine root growth to increase the FRLD and develop into deeper soil layers. However, due to the prolonged exposure to a high level of salinity, their roots below 30 cm depth senesced prematurely, leading to an inhibition in shoot growth and also resulting in yield reduction of 10.99% and 11.75%, compared to CK, respectively. Furthermore, the S1-2-3 and S1-3-3 treatments produced more reasonable distributions of FRLD, which did not boost fine root growth but established fewer weak areas (FLRD < 0.66 cm-3) in their root systems. Moreover, the S1-2-3 treatment contributed to increasing leaf development and biomass accumulation, compared to CK, whereas it allowed for minimizing yield reduction. Therefore, our study proposed the S1-2-3 treatment as the recommended training mode for summer maize while utilizing brackish water resources.

CDC42-mediated Wnt signaling facilitates odontogenic differentiation of DPCs during tooth root elongation

BackgroundCDC42 is a member of Rho GTPase family, acting as a molecular switch to regulate cytoskeleton organization and junction maturation of epithelium in organ development. Tooth root pattern is a highly complicated and dynamic process that dependens on interaction of epithelium and mesenchyme. However, there is a lack of understanding of the role of CDC42 during tooth root elongation.MethodsThe dynamic expression of CDC42 was traced during tooth development through immunofluorescence staining. Then we constructed a model of lentivirus or inhibitor mediated Cdc42 knockdown in Herwig’s epithelial root sheath (HERS) cells and dental papilla cells (DPCs), respectively. Long-term influence of CDC42 abnormality was assessed via renal capsule transplantation and in situ injection of alveolar socket.ResultsCDC42 displayed a dynamic spatiotemporal pattern, with abundant expression in HERS cells and apical DPCs in developing root. Lentivirus-mediated Cdc42 knockdown in HERS cells didn’t disrupt cell junctions as well as epithelium-mesenchyme transition. However, inhibition of CDC42 in DPCs undermined cell proliferation, migration and odontogenic differentiation. Wnt/β-catenin signaling as the downstream target of CDC42 modulated DPCs’ odontogenic differentiation. The transplantation and in situ injection experiments verified that loss of CDC42 impeded root extension via inhibiting the proliferation and differentiation of DPCs.ConclusionsWe innovatively revealed that CDC42 was responsible for guiding root elongation in a mesenchyme-specific manner. Furthermore, CDC42-mediated canonical Wnt signaling regulated odontogenic differentiation of DPCs during root formation.

Estimation of generalized soil structure index based on differential spectra of different orders by multivariate assessment

Better soil structure promotes extension of plant roots thereby improving plant growth and yield. Differences in soil structure can be determined by changes in the three phases of soil, which in turn affect soil function and fertility levels. To compare the quality of soil structure under different conditions, we used Generalized Soil Structure Index (GSSI) as an indicator to determine the relationship between the “input” of soil three phases and the “output” of soil structure. To achieve optimum monitoring of comprehensive indicators, we used Successive Projections Algorithm (SPA) for differential processing based on 0.0–2.0 fractional orders and 3.0–10.0 integer orders and select important wavelengths to process soil spectral data. In addition, we also applied multivariate regression learning models including Gaussian Process Regression (GPR) and Artificial Neural Network (ANN), exploring potential capabilities of hyperspectral in predicting GSSI. The results showed that spectral reflection, mainly contributed by long-wave near-infrared radiation had an inverse relationship with GSSI values. The wavelengths between 404-418 nm and 2193–2400 nm were important GSSI wavelengths in fractional differential spectroscopy data, while those ranging from 543 to 999 nm were important GSSI wavelengths in integer differential spectroscopy data. Also, non-linear models were more accurate than linear models. In addition, wide neural networks were best suited for establishing fractional-order differentiation and second-order differentiation models, while fine Gaussian support vector machines were best suited for establishing first-order differentiation models. In terms of preprocessing, a differential order of 0.9 was found as the best choice. From the results, we propose that when constructing optimal prediction models, it is necessary to consider indicators, differential orders, and model adaptability. Above all, this study provided a new method for an in-depth analyses of generalized soil structure. This also fills the gap limiting the detection of soil three phases structural characteristics and their dynamic changes and provides a technical references for quantitative and rapid evaluation of soil structure, function, and quality.