Anti-aging Research Articles

Evolution of Interfacial Hydration Structure Induced by Ion Condensation and Correlation Effects

Interfacial hydration structures are crucial in wide‐ranging applications, including battery, colloid, lubrication. Multivalent ions like Mg2+ and La3+ show irreplaceable roles in these applications, which are hypothesized due to their unique interfacial hydration structures. However, this hypothesis lacks experimental supports. Here, we provide the first observation for their interfacial hydration structures with molecular resolution using atomic force microscopy. We observed the evolution of layered hydration structures at La(NO3)3 solution‐mica interfaces. As concentration increases from 25 mM to 2 M, the layer number varies from 2 to 1 and back to 2, and the interlayer thickness rises from 0.25±0.05 to 0.34±0.03 nm, with hydration force increasing from 0.27±0.07 to 1.04±0.24 nN. Theory and molecular simulation reveal that the cations form inner‐sphere complexes. Multivalence induces concentration‐dependent ion condensation and correlation effects, resulting in compositional and structural evolution within interfacial hydration structures. Additional experiments at seven different solid‐liquid interfaces together with literature comparison confirm the universality of this mechanism for both multivalent and monovalent ions. New factors affecting interfacial hydration structures are revealed, including concentration and solvent dielectric constant. This insight provides guidance for designing interfacial hydration structures to optimize solid‐liquid‐interphase for battery life extension, modulate colloid stability and develop efficient lubricants.

Evolution of Interfacial Hydration Structure Induced by Ion Condensation and Correlation Effects.

Interfacial hydration structures are crucial in wide-ranging applications, including battery, colloid, lubrication. Multivalent ions like Mg2+ and La3+ show irreplaceable roles in these applications, which are hypothesized due to their unique interfacial hydration structures. However, this hypothesis lacks experimental supports. Here, we provide the first observation for their interfacial hydration structures with molecular resolution using atomic force microscopy. We observed the evolution of layered hydration structures at La(NO3)3 solution-mica interfaces. As concentration increases from 25 mM to 2 M, the layer number varies from 2 to 1 and back to 2, and the interlayer thickness rises from 0.25±0.05 to 0.34±0.03 nm, with hydration force increasing from 0.27±0.07 to 1.04±0.24 nN. Theory and molecular simulation reveal that the cations form inner-sphere complexes. Multivalence induces concentration-dependent ion condensation and correlation effects, resulting in compositional and structural evolution within interfacial hydration structures. Additional experiments at seven different solid-liquid interfaces together with literature comparison confirm the universality of this mechanism for both multivalent and monovalent ions. New factors affecting interfacial hydration structures are revealed, including concentration and solvent dielectric constant. This insight provides guidance for designing interfacial hydration structures to optimize solid-liquid-interphase for battery life extension, modulate colloid stability and develop efficient lubricants.

Apallic syndrome of toxic origin: the diagnostic criteria

Background. The global epidemic of strong synthetic opioids and “new” psychoactive substances has increased the number of overdoses that cause prolonged coma with subsequent persistent vegetative state or apallic syndrome. The purpose was to investigate the clinical manifestations, functional and pathohistological changes of the brain in apallic syndrome due to severe drug poisoning (overdose). Materials and methods. A retrospective analysis of the medical data of six people aged 21–38 years with drug poisoning involving illegal methadone, psychostimulants, barbiturates and alcohol was carried out. Results. Among the patients of the toxicology department of the Kyiv City Clinical Emergency Hospital in 2008–2020, an increase in the number of cases of apallic syndrome was noted from 0.025 to 0.14 ‰, which correlates with mass poisonings with illegal methadone. A key role in the diagnosis of apallic syndrome of toxic origin belongs to the neurological and behavioural examination of the patient, which should be carried out by experienced neurologists specialising in counselling people with poisoning. Functional magnetic resonance imaging and positron emission tomography are the modern standard for diagnosing vegetative states; however, these methods are still difficult to access in Ukraine. The visualization zones of the pathological process in the brain in case of toxic damage are mainly basal ganglia and/or thalamus and dentate nucleus, cortical zone of grey matter, periventricular zone of white matter, corticospinal tract and corpus callosum; asymmetric white matter damage as a sign of demyelination; parietal-occipital subcortical vasogenic edema and lesions of the central pons are characteristic. Forensic and histological studies of the cerebral cortex revealed diffuse loss of neurons, neuronophagia, satellitosis, deformed pyramidal neurons with apical dendrites and manifestations of homogenising necrosis, angiomatosis microfoci and neuropil vacuolation, mineralization of the tissue and a moderate glial reaction around the petrifications. Conclusions. For the health care system of Ukraine, it remains relevant to improve the existing definitions and diagnostic criteria of the apallic syndrome, which attracts the attention of doctors in connection with an increase in cases of life extension of patients after waking up from a long coma.

Impact of Red Wine Vinegar-Based Solution on Bacterial Communities of Squid and Shrimp Skewers: A Classic and Genomic Approach

AbstractSeafood is an essential component of a balanced and healthy diet, which increases its demand. However, its biological composition and high moisture content make these products extremely perishable. To prevent spoilage, and the consequent food waste and financial expenses throughout the seafood supply chain, new technologies have been successfully developed to inhibit bacterial growth, the main cause of seafood spoilage. This work aimed to test a shelf life extension technique for seafood skewers whilst maintaining an all-natural label using a financially feasible red wine vinegar treatment applied by immersion or pulverisation. Bacterial growth was monitored by classical methods and by 16S rRNA gene amplicon sequencing during the 5 days of storage. Immersion of samples in a vinegar-based solution effectively reduced Pseudomonas and Enterobacterales counts (by 2 log cfu/g), immediately after application and throughout storage. The overall structure and diversity of the bacterial community were analysed, and a strong reduction in bacterial diversity and impact on bacterial composition was observed immediately after immersion in the red wine vinegar solution. In untreated samples, Pseudomonadota (especially the Gammaproteobacteria class) was the principal phylum, whereas the microbiota of the treated samples was dominated by Bacillota (mainly the Bacilli class). Sensory analysis revealed a mild vinegar or vinaigrette flavour in treated samples; however, these characteristics were not unpleasant. Although applying a vinegar-based solution by immersion promoted a significant reduction in the growth of spoilage bacteria during the first days of storage, further tests are required to confirm the shelf life extension.

A Review on Towards Long Lifetime Battery: AI Based Manufacturing and Management

A Battery Management System (BMS) is crucial for maintaining the health and efficiency of batterypowered devices. Integrating Artificial Intelligence (AI) into BMS offers advanced capabilities for optimizing battery performance. AI algorithms analyze real-time data from the battery, such as voltage, temperature, and charge cycles, to predict battery life, enhance safety, and improve energy efficiency.AI can detect patterns and anomalies that traditional systems might miss, allowing for proactive adjustments to charging and discharging processes. This helps prevent overcharging, overheating, and deep discharges, which can damage batteries and reduce their lifespan. Additionally, AIdriven BMS can learn from historical data to refine its predictions and adapt to changing conditions. It also supports better energy management in electric vehicles and renewable energy systems, where efficient battery use is critical. By continuously monitoring and optimizing battery performance, AI enhances the overall reliability and longevity of battery systems, making technology more sustainable and efficient. Thus, the use of AI in BMS represents a significant advancement in battery technology, contributing to improved performance and safety in various applications. This study explores battery management systems by optimizing performance, extending lifespan, and improving efficiency and ensure safety through predictive analysis and real-time monitoring. Keywords: Battery Management system, artificial intelligence, state of charge, state of health, battery life extension, fault detection.

Transcriptional analysis of C. elegans fmos at different life stages and their roles in ageing

Flavin-containing monooxygenases (FMOs) are present in most organisms including plants, fungi, bacteria, invertebrates and vertebrates, where they catalyse the oxidative metabolism of a range of xenobiotics and endogenous metabolites. FMOs have been associated with ageing and longevity in the mouse and in C. elegans. As all five FMOs of C. elegans share an evolutionary root with mouse and human FMO5, it was of interest to discover if effects on ageing and longevity persisted across the whole group. We therefore investigated the impact of fmo gene knockout (KO) in C. elegans. We found that fmo-1, fmo-3 and fmo-4 KO significantly extended C. elegans lifespan relative to wild type and, as previously reported, FMO-2 over-expression did likewise. The transcription levels of C. elegans fmo genes were determined throughout the life cycle (embryo, larva and adult) in wild type and in each mutant to discover if their expression was related to stages in ageing, and expression levels were compared to those in human and mouse. In wild type worms, fmo-1 and fmo-4 were the mostly highly transcribed genes (especially at the larval stage), whereas fmo-2 and fmo-3 were the least transcribed, at all stages. Notably, the knockout of fmo-4 led to a 17- to 30-fold up-regulation of fmo-2, along with significantly increased levels of the other fmos. This parallels recent findings in the long-lived C. elegans tald-1 mutant where fmo-2 was also significantly up-regulated and reinforces its importance in lifespan extension.

An interlinked dynamic model of timber and carbon stocks in Japan's wooden houses and plantation forests

Carbon absorption in growing trees is an important element of a carbon-neutral society, and the long-term storage of carbon stocks is a crucial sustainability challenge. Previous studies have focused on either live-biomass carbon stocks in plantation forests or anthropogenic carbon stocks in man-made objects. For a comprehensive nature-based climate solution, an analytical framework, dataset, and scenario setup for modeling the interrelationship between timber supply and demand are required. This study developed an interlinked material flow analysis model in which the timber demand for wooden houses is connected with timber supply from managed plantation forestry. We demonstrate the model by quantifying both live-biomass and anthropogenic carbon stocks and their potentials in Japan. We compared multiple scenario-runs of the model for wooden house demands estimated by population change with varying combinations of house types, structures, and lifespans. Our results show that carbon stocks will reach a maximum amount of 1.1 billion t-C by 2050 in a scenario of high demand for wooden detached houses with lifespan extensions. On the other hand, we also found that the aging of plantation forests and their reduced carbon-stocking capacities appear inevitable in any scenario owing to the limited demand for timber. Notably, despite the widely different settings of the various scenarios, our results exhibited narrow variances in future potential carbon storage in Japan. This can be explained by the unique population characteristics and building demographics of Japan. These counterintuitive findings highlight the need for interrelated modeling of the forestry and construction sectors. Our model and its scope are versatile and applicable to other case study areas, estimation periods, and target materials.

Life Extension of Wind Turbine Drivetrains by means of SCADA Data: Case Study of Generator Bearings in an Onshore Wind Farm

Accurate predictions for remaining useful life (RUL) of wind turbine drivetrains is crucial in reducing downtime, optimising maintenance strategies, extending operational life and improving costs. The purpose of this study is to present a method which utilises SCADA data for RUL estimation. It aims to answer whether, by only having the basic SCADA data, normally collected from all wind turbines, valuable RUL prediction results can be obtained. The work intends to develop a reliable, accurate, user-friendly and informative tool to enable observation of any growing trends in the proposed metrics, such as temperature difference, cumulative sum of temperature difference and the moving average of the cumulative sum. These metrics are defined based on the differences between the actual temperatures of the components, that might have undergone some damage and the model predicted temperatures of those components if they would have remained healthy, throughout years of operation. A machine learning model is used, along with selected SCADA input parameters, to predict the healthy state temperature of components. The proposed method is implemented on SCADA data collected from an actual wind farm over seven years. The results of this study show that while the SCADA data analysis can contribute to fault detection, the estimation of RUL purely based on SCADA appears to be uncertain. Such tools however, can be used as a monitoring method, during operation, to record abnormality trend of the components over the years and can be used as important inputs for the life extension evaluation of wind turbine drivetrains.

Bias Identification Approaches for Model Updating of Simulation-based Digital Twins of Bridges

. Simulation-based digital twins of bridges have the potential not only to serve as monitoring devices of the current state of the structure but also to generate new knowledge through physical predictions that allow for better-informed decisionmaking. For an accurate representation of the bridge, the underlying models must be tuned to reproduce the real system. Nevertheless, the necessary assumptions and simplifications in these models irremediably introduce discrepancies between measurements and model response. We will show that quantifying the extent of the uncertainties introduced through the models that lead to such discrepancies provides a better understanding of the real system, enhances the model updating process, and creates more robust and trustworthy digital twins. The inclusion of an explicit bias term will be applied to a representative demonstrator case based on the thermal response of the Nibelungenbrücke of Worms. The findings from this work are englobed in the initiative SPP 100+, whose main aim is the extension of the service life of structures, especially through the implementation of digital twins.

Biological function of EPHB4 in the aging process of vascular endothelial cells: mtDNA molecular mechanism and MAPK/PGC-1/TFAM signaling pathway.

Previous studies have shown that EPHB4 is also involved in regulating the proliferation, migration, and apoptosis of endothelial cells. In this study, we found a close relationship between EPHB4 and aging. Therefore, in-depth research on the relationship between EPHB4 and aging can help reveal the molecular mechanisms of aging and provide new ideas and methods for developing anti-aging drugs and treating vascular aging-related diseases. In addition, in our current study, we found a close relationship between EPHB4, cellular senescence, and CM-AVM. The MAPK/PGC-1/TFAM signaling axis mediated by EPHB4 may also be involved in the process of CM-AVM, laying a solid foundation for future in-depth studies on the relationship between EPHB4 and CM-AVM. Our findings revealed a decrease in mitochondrial membrane potential associated with EPHB4 deficiency, suggesting that EPHB4 loss may contribute to mitochondrial dysfunction. Additionally, EPHB4 deficiency led to an elevation in mitochondrial ROS levels, which was confirmed using mitochondrial-specific fluorescent probes. Furthermore, EPHB4 deficiency resulted in down-regulated expression of NRF1 and SOD2, which could be a significant contributor to mitochondrial oxidative stress. To validate this hypothesis, we conducted rescue experiments by restoring PGC-1 expression. The results showed a partial recovery of mitochondrial membrane potential and a reduction in cell senescence. These findings suggest that EPHB4 regulates mitochondrial functional integrity through the MAPK/PGC-1/TFAM signaling axis.

Preparation of polyvinyl alcohol/carboxymethyl cellulose sodium/chitosan paper-based antimicrobial indicator cards using mixed anthocyanin with stability-colorimetric sensitivity: Monitoring freshness of carp

In this study, an anthocyanin solution with both high stability and sensitivity was successfully prepared through a blending method, and the optimal ratio was determined. Ultraviolet-visible spectroscopy analysis demonstrated that an increase in the proportion of black bean peel anthocyanins resulted in a deeper color and a more pronounced color change effect. Moreover, the stability of the blended anthocyanins was markedly enhanced with an increase in the proportion of purple cabbage anthocyanins, resulting in a slower decomposition rate under light, temperature, oxidation, and varying pH conditions. The blended anthocyanins, employed as a pH indicator, were utilized to prepare an antibacterial indicator card. It was observed that the mechanical properties of the coated paper remained unaltered by the ratio of the mixed pigments. The mixed pigments enhanced the stability of the antibacterial indicator card with respect to environmental fluctuations while facilitating more rapid and sensitive color transitions in the presence of ammonia and under disparate pH conditions. The application of indicator cards with five different anthocyanin ratios to fish resulted in a shelf life extension of 1–2 days compared to the control group and enabled real-time monitoring of fish freshness. A comprehensive evaluation demonstrated that the optimal indicator performance was attained when the mass ratio of black bean peel anthocyanins to purple cabbage anthocyanins was 1:1.

High-Speed Train Wheel Set Bearing Analysis: Practical Approach to Maintenance Between End of Life and Useful Life Extension Assessment

High-Speed Train Wheel Set Bearing Analysis: Practical Approach to Maintenance Between End of Life and Useful Life Extension Assessment

Exploring the frontiers of cryonics: Feasibility, benefits, and future impact on humanity

This paper explores the scientific, ethical, and technological dimensions of cryonics—the preservation of human remains at ultra-low temperatures with the hope of future revival when medical advancements permit. Originating from Robert Ettinger's 1960s proposition, cryonics challenges traditional notions of mortality. This study examines current cryonics technology, particularly vitrification methods used to prevent ice crystal formation during freezing, as practiced by institutions like the Cryonics Institute and Alcor Life Extension Foundation. While successful human revival has yet to be achieved, progress in preserving cells and organs offers valuable insights. The feasibility is assessed by analyzing scientific possibilities and limitations, ethical and legal considerations, and technological challenges such as cellular damage and the complexities of restoring consciousness. Potential benefits include medical and therapeutic advancements, life extension, improved organ preservation, and psychological and societal impacts. Revival prospects are explored through future technologies like nanotechnology and regenerative medicine, alongside statistical probabilities and expert predictions. The paper concludes by emphasizing the need for interdisciplinary collaboration, ethical frameworks, and continued research to navigate challenges and responsibly harness the potential of cryonics, ultimately shaping its future role in humanity.

Nanotechnology in floriculture: Extending vase life and improving postharvest quality

Cut flowers are traditionally used worldwide, regardless of caste, creed, and religion, especially in Asian countries. The global trade of cut flowers has shown a substantial increase in recent years and is expected to persist due to the promotion and application of horticulture plants for their various advantages . The quality of vase life is critical in ensuring customer satisfaction and encouraging repeat purchases. Effective postharvest management is essential for enhancing the quality and extending the shelf life of cut flowers. With proper handling, cut flowers can last several days in a vase. Nanotechnology presents innovative solutions for postharvest management, especially in the cut flower sector. Specifically, Nanoparticles have been utilized in packaging to act as ethylene inhibitors and antimicrobial agents, contributing to the extension of cut flower vase life. One of the strongest ethylene perceptions is 1-methylcyclopropene (1-MCP), a gaseous and nontoxic that binds to ethylene receptors irreversibly, blocking the action of ethylene. Nano-Silver particles improve postharvest longevity by increasing water absorption rather than transpiration. Additionally, Nano-Selenium enhances the water balance in cut flowers. This review describes how nanoparticles suppress microbial growth and block ethylene action in cut flowers, extending their vase life.

Improvement of the safety of scallops through the application of calcium alginate films with the controlled release of Vibrio bacteriophage

The increase in the population of antibiotic-resistant bacteria has prompted the exploration of food safety alternatives, such as bacteriophages (phages). This study aimed to develop anti-Vibrio films with controlled phage release and focused on the shelf life extension of scallops. VPy01, a newly isolated lytic phage, demonstrated a rapid bactericidal effect against V. parahaemolyticus in 1 h at a multiplicity of infection of 1. It was stable in a wide range of temperature (-18°C to 60°C), pH (4−11), and salinity (0–6%) conditions. As a genetically secure agent, VPy01 was loaded into a sodium alginate (SA, 2%, w/v) film, and glycerol was used as a plasticizer. It was then cross-linked with various calcium salts (CaCl2, CaCO3, hydroxyapatite, and CaSO4). The SA film cross-linked with CaCl2 (SA-CaCl2) efficiently released VPy01, showing remarkable vibricidal effects (>2-log CFU). The SA-CaCl2 film with well-dispersed phages improved hardness, elongation at break, and oxygen barrier ability. When applied to scallops, the SA-CaCl2 film preserved the hardness and weight of scallops and reduced Vibrio by 1.8-log CFU/scallop. Thus, their shelf life was extended. Overall, this study highlighted the potential of VPy01-loaded calcium alginate films for the extension of scallop shelf life, contributing to sustainability in the seafood industry.

A novel edible biocomposite coating based on alginate from the brown seaweed Dictyota mertensii loaded with beeswax nanoparticles extends the shelf life of yellow passion fruit

In this study, an edible biocomposite coating of alginate extracted from the brown seaweed Dictyota mertensii was loaded with beeswax nanoparticles (BDMAB) to extend the shelf life of yellow passion fruit (Passiflora edulis f. flavicarpa). The films were characterized by morphology, moisture content, contact angle, water vapor permeability, solubility, and optical and mechanical properties. Using a 4 × 6 factorial design, coated fruit was evaluated during six storage intervals (0, 2, 4, 6, 8, 10 days) at 22.5 ± 0.5 °C and 65 ± 5 % RH, focusing on respiration rate, weight loss, peel thickness and color, pulp yield and color, soluble solids, titratable acidity, ascorbic acid, phenolics, antioxidant capacity, and sensory acceptance. The BDMAB coating, composed of 67.9 % (w/w) ADM (bleached), 5 % (w/w) glycerol, 8.5 % (w/w) beeswax, and 18.6 % (w/w) Tween 80, significantly (p < 0.05) reduced the respiration rate, minimized weight loss, and preserved quality attributes, such as acidity, ascorbic acid, phenols, and antioxidant capacity. A 3-day extension in shelf life was inferred based on the climacteric peak delay of BDMAB-coated fruits compared with the control. Sensory analysis confirmed the acceptance of BDMAB coating. Therefore, BDMAB biocomposite coatings have great potential for preserving yellow passion fruit and promoting sustainability and conservation.

Processing Fresh-Cut Potatoes Using Non-Thermal Technologies and Edible Coatings

The increasing consumer demand for minimally processed and ready-to-cook food products has elevated the significance of fresh-cut potatoes, which offer health benefits, high sensory properties, and convenience. However, extending the shelf life of fresh-cut potatoes while preserving their organoleptic qualities remains a significant challenge. This review examines the effectiveness of emerging non-thermal technologies, such as osmotic dehydration (OD), high-pressure processing (HPP), pulsed electric field (PEF), and ohmic heating (OH), in processing fresh-cut potatoes. Among these, HPP and PEF have shown particular promise in extending shelf life and preserving sensory attributes, while OD and OH present advantages in maintaining nutritional quality. However, challenges such as high energy consumption, equipment costs, and industrial scalability limit their broader application. The use of natural preservatives and edible coatings is also explored as a means to enhance product quality and address the demand for clean-label foods. Further research is needed to optimize these technologies for large-scale production, reduce energy usage, and explore combined approaches for improved shelf life extension. This comprehensive review provides a critical analysis of the operational parameters of these technologies and their impact on the quality and shelf life of fresh-cut potatoes, identifying current research gaps and proposing directions for future studies.

Enhancing tomato shelf life using isolated cellulose fibers from Asian Palmyra palm coated with garlic oil

In this study, garlic-oil-combined cellulose fibers were prepared by using Borassus flabellifer (Asian Palmyra palm) to enhance the post-harvest shelf life of tomatoes. The physicochemical properties of the prepared cellulose fibers were characterized by using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FE-SEM). The B. flabellifer cellulose fibers combined with garlic oil (BCF/GO) coatings exhibited significant antifungal properties against Aspergillus flavus. In addition, the BCF/GO coating resulted in a notable extension in the shelf life of tomatoes regarding parameters such as weight loss, firmness, pH, ascorbic acid content, lycopene level, moisture content, and titratable acidity after 25 days of storage at 25–29 °C with 85 % relative humidity. The synergistic combination of BCF and GO presents a natural and sustainable solution for extending the shelf life of tomatoes with the potential to significantly reduce post-harvest losses in the food industry.

The heat of longevity: sex differences in lifespan and body temperature.

Dietary restriction (DR) has long been recognized as a powerful intervention for extending lifespan and improving metabolic health across species. In laboratory animals, DR-typically a 30%-40% reduction in caloric intake-delays aging and enhances mitochondrial function, oxidative defense, and anti-inflammatory pathways. In humans, findings from the CALERIE™ trial confirm DR's potential benefits, with a 25% caloric reduction over 2years resulting in reduced visceral fat, improved cardiometabolic health, and favorable gene expression changes linked to proteostasis, DNA repair, and inflammation. However, recent research in genetically diverse mouse populations reveals that the impact of DR on lifespan is substantially modulated by genetic background, underscoring the importance of individual variability. Additionally, emerging evidence challenges previous assumptions that lower body temperature universally benefits lifespan extension, with data indicating complex relationships between thermoregulation, sex, and longevity. These findings underscore the need for nuanced approaches to DR in both research and potential therapeutic applications, with considerations for genetic and sex-specific factors to maximize healthspan and lifespan outcomes.

Patchouli essential oil extends the lifespan and healthspan of Caenorhabditis elegans through JNK-1/DAF-16

AimsPatchouli essential oil (PEO) is the major active ingredient of a famous medicinal plant Pogostemon cablin (Blanco) Benth. This study aims to investigate the anti-aging activities of PEO and its major component, and elucidate the underlying molecular mechanisms. Main methodsThe anti-aging activities of PEO and its main component patchouli alcohol (PA) were investigated by examining the lifespan, senescence associated indicators as well as stress resistance of Caenorhabditis elegans. RNA-Sequencing was performed to analyze differentially expressed genes and the enrichments of GO and KEGG pathways in nematodes treated with PEO. The potential anti-aging target was predicted using a network pharmacology method and molecular docking. The underlying mechanism of senescence-delaying action was explored using C. elegans mutants and GFP transgenic strains. Key findingsPEO modulated lifespan and healthspan extension of worms, ameliorated the senescence characterizations, and increased the survival in stress resistance assays. PEO reduced spawning, lipid accumulation and reactive oxygen species (ROS) levels of nematodes. The levels of anti-oxidative genes and proteins were obviously upregulated after PEO treatment. Moreover, PA was identified to be an ingredient for PEO-mediated nematode longevity. The JNK-1/DAF-16 signaling pathway played a critical role in PEO/PA-mediated longevity. SignificanceThe findings revealed that PEO and its major component PA showed significant anti-aging activity through modulating the JNK-1/DAF-16 signaling pathway, which provides a promising strategy to treat aging and age-related diseases.