Carbohydrate Metabolism Gene Expression Research Articles

Genome-wide analysis of Anisakis simplex sensu lato: the role of carbohydrate metabolism genes in the parasite’s development

Anisakis simplex sensu lato is a parasitic nematode which can cause gastric symptoms and/or allergic reactions in humans who consume raw and undercooked fish. Anisakiasis poses a growing health problem around the globe because it causes non-specific symptoms and is difficult to diagnose. This genome-wide study was undertaken to expand our knowledge of A. simplex s.l. at the molecular level and provide novel data for biological and biotechnological research into the analyzed species and related nematodes. A draft genome assembly of the L3 stage of A. simplex s.l. was analyzed in detail, and changes in the expression of carbohydrate metabolism genes during the parasite's life cycle were determined. To our knowledge, this is the first genome to be described for a parasitic nematode of the family Anisakidae to date. We identified genes involved in parasite-specific pathways, including carbohydrates metabolism, apoptosis and chemo signaling. A total of 7607 coding genes were predicted. The genome of A. simplex s.l. is highly similar to genomes of other parasitic nematodes. In particular, we described a valuable repository of genes encoding proteins of trehalose and glycogen metabolism, and we developed the most comprehensive data set relating to the conversion of both saccharides which play important roles during the parasite's life cycle in a host environment. We also confirmed that trehalose is synthesized at the expense of glycogen. Trehalose anabolism and glycogen catabolism were the predominant processes in stages L4 and L5, which could confirm our and other authors’ previous reports that trehalose is synthesized at the expense of glycogen. The A. simplex s.l. genome provides essential data for post-genomic research into the biology of gastrointestinal and allergic anisakiasis in humans and the biology of other important parasitic helminths.

Effects of Karela (Bitter Melon; Momordica charantia) on genes of lipids and carbohydrates metabolism in experimental hypercholesterolemia: biochemical, molecular and histopathological study

BackgroundHypercholesterolemia is a serious diseases associated with type-2 diabetes, atherosclerosis, cardiovascular disorders and liver diseases. Humans seek for safe herbal medication such as karela (Momordica charantia/bitter melon) to treat such disorders to avoid side effect of pharmacotherapies widely used.MethodsForty male Wistar rats were divided into four equal groups; control group with free access to food and water, cholesterol administered group (40 mg/kg BW orally); karela administered group (5 g /kg BW orally) and mixture of cholesterol and karela. The treatments continued for 10 weeks. Karela was given for hypercholesterolemic rats after 6 weeks of cholesterol administration. Serum, liver and epididymal adipose tissues were taken for biochemical, histopathological and genetic assessments.ResultsHypercholesterolemia induced a decrease in serum superoxide dismutase (SOD), catalase, reduced glutathione (GSH) and an increase in malondialdehyde (MDA) levels that were ameliorated by karela administration. Hypercholesterolemia up regulated antioxidants mRNA expression and altered the expression of carbohydrate metabolism genes. In parallel, hypercholesterolemic groups showed significant changes in the expression of PPAR-alpha and gamma, lipolysis, lipogenesis and cholesterol metabolism such as carnitine palmitoyltransferase-1 (CPT-1). Acyl CoA oxidase (ACO), fatty acids synthase (FAS), sterol responsible element binding protein-1c (SREBP1c), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoAR) and cholesterol 7α-hydroxylase (CYP7A1) at hepatic and adipose tissue levels. Interestingly, Karela ameliorated all altered genes confirming its hypocholesterolemic effect. Histopathological and immunohistochemical findings revealed that hypercholesterolemia induced hepatic tissue changes compared with control. These changes include cholesterol clefts, necrosis, karyolysis and sever congestion of portal blood vessel. Caspase-3 immunoreactivity showed positive expression in hepatic cells of hypercholesterolemic rats compared to control. All were counteracted and normalized after Karela administration to hypercholesterolemic group.ConclusionCurrent findings confirmed that karela is a potential supplement useful in treatment of hypercholesterolemia and its associated disorders and is good for human health.

Temperature-dependent regulation of sugar metabolism in wild-type and low-invertase transgenic chipping potatoes during and after cooling for low-temperature storage

Regulation of sugar metabolism in potato tubers stored at low temperatures has significant ramifications for producers and consumers of potato chips and French fries. Low-temperature storage reduces losses due to sprouting and disease but induces undesirable accumulation of the reducing sugars glucose and fructose. These sugars react with free amino acids during frying to produce dark-colored and bitter-tasting products that have elevated levels of acrylamide, a suspected human carcinogen. Elevated amounts of tuber glucose and fructose are produced when vacuolar acid invertase (VInv) hydrolyses sucrose derived from starch degradation. In this study, wild-type tubers and tubers in which VInv expression was reduced by RNA interference were used to study the temperature-dependent regulation of sugar accumulation and expression of carbohydrate metabolism genes during and after the cooling of tubers to 9 or 3°C. It was found that transcriptional control of genes central to carbohydrate metabolism occurred in three overlapping phases. First, early in cooling, slight increases in VInv and β-amylase expression occurred. Second, at lower temperatures, large decreases in AGPase and GBSS expression were observed. Finally, when tubers reached 3–5°C, large increases in β-amylase expression were observed, coinciding with large increases in sucrose in all genotypes and large increases in glucose and fructose in tubers with high VInv expression. Suppression of VInv expression did not alter the expression of β-amylase, AGPase, GBSS, or other genes, indicating that there was little or no feedback from reducing sugars to transcriptional control of carbohydrate metabolism. Invertase activity of WT tubers stored at 9°C was comparable to that of WT tubers stored at 3°C, but reducing sugars were markedly higher at the lower temperature. Taken together, these data support a model in which reducing sugar accumulation is controlled both by temperature-regulated changes in sucrose accumulation, resulting from increased starch degradation by β-amylase and decreased starch resynthesis by AGPase and GBSS, and by differences in VInv activity that persist throughout storage.

SUN-PP040: Hypoxia-Induced Expression of Carbohydrate Metabolism Genes is Enhanced by Dietary Citric Acid

SUN-PP040: Hypoxia-Induced Expression of Carbohydrate Metabolism Genes is Enhanced by Dietary Citric Acid

Changes in the expression of carbohydrate metabolism genes during three phases of bud dormancy in leafy spurge

Underground adventitious buds of leafy spurge (Euphorbia esula) undergo three well-defined phases of dormancy, para-, endo-, and ecodormancy. In this study, relationships among genes involved in carbohydrate metabolism and bud dormancy were examined after paradormancy release (growth induction) by decapitation and in response to seasonal signals. Real-time PCR was used to determine the expression levels of carbohydrate metabolism genes at different phases of bud dormancy. Among differentially-regulated genes, expression of a specific Euphorbia esula beta-amylase gene (Ee-BAM1) increased 100-fold after growth induction and 16,000-fold from July (paradormancy) to December (ecodormancy). Sequence data analysis indicated that two genes, Ee-BAM1 and Ee-BAM2, could encode this beta-amylase. However, real-time PCR using gene-specific primer pairs only amplified Ee-BAM1, indicating that Ee-BAM2 is either specific to other organs or not abundant. The deduced amino acid sequences of these two genes are very similar at the N-terminal but differ at the C-terminal. Both contain a nearly identical, predicted 48-amino acid plastid transit peptide. Immunoblot analyses identified a 29 kD (mature Ee-BAM1 after cleavage of the transit peptide) and a 35 kD (unprocessed EeBAM1) protein. Both 35 and 29 kD proteins were constitutively expressed in growth-induced and seasonal samples. Immunolocalization indicated that Ee-BAM1 is in the cytosol of cells at the shoot tip of the bud. Ee-BAM1 also surrounds the amyloplasts in mature cells toward the base of the bud. These observations suggests that Ee-BAM1 may have dual functions; serving as reserve protein in the cytosol and as a degrading enzyme at the surface of amyloplasts.