In the present study, directional and spatial variations in the mechanical properties are calculated in two nuclear-grade materials. In practice, multiple ASTM standard specimens are tested to measure mechanical properties of any material. The variations obtained in the properties during the tests are generally neglected assuming such variations are due to experimental uncertainties. However, such variations may indicate some degree of anisotropy and spatial inhomogeneity in the material due to component fabrication. In the present study, multiple miniaturized tensile specimens are tested. These specimen materials are taken across the thickness and at different geometrical locations in the two manufactured nuclear-grade components. The experimental load versus displacement data of all the specimens are then used to evaluate stress-strain data and cohesive zone parameters. These parameters are determined for each tested specimen separately to gather variations over the geometries of the components. Subsequently, TPB specimens are analyzed employing these parameters to calculate variations in fracture initiation toughness over the geometry. The key findings of the present work include higher strengths in circumferential direction in comparison to the longitudinal direction for SA333 Gr6 steel. A new equation is developed to correlate the material toughness with the fracture toughness with a proportionality constant of 2.7778 for low-alloy carbon steels. The study showed that directional and spatial variations in Jini are less pronounced in 20MnMoNi55 compared to SA333Gr6 materials. This finding is crucial for safety analyses in nuclear components and indicates that this methodology can be applied more widely across different materials.