Experimental Boxes Research Articles

Analyzing the Effects of Monochromatic Lights on the Fungal Growth to Control the Progression of Microbial Deterioration on Animal Collections Preserved in the Zoological Museum of Naples, Italy

ABSTRACT Exposing artworks and artifacts in museums is fundamental to disseminate culture and knowledge but is also risky since the exposure to environmental conditions can deteriorate exhibited objects. Light is crucial, being able to trigger photochemical effects, and jointly responsible (with temperature and humidity) for biological risk generation. In this context, fungal growth in museum artifacts is a damaging factor that should not be underestimated. Consequently, the study of lighting effects on fungi development is essential. In this work, five different fungal strains (Aspergillus fumigatus, Bjerkandera adusta, Fusarium oxysporum, Penicillium chrysogenum, and Penicillium granulatum) isolated from samplings on animal exhibits hosted in the Zoological Museum of Naples, Italy, are cultivated inside four experimental boxes, equipped with monochromatic LEDs with different peak wavelengths (460 nm, 518 nm, 594 nm, and 638 nm). The growth rate, the protein and carbohydrate contents are analyzed and compared to results obtained in darkness (typical growth conditions for studies about fungi). Results demonstrate that, even if fungi are heterotrophic organisms and do not need light to grow and develop, their growth rate, and their protein and carbohydrate contents are affected by the exposure to monochromatic radiation, but the spectral sensitivity strictly depends on the analyzed species. For this reason, it is not possible to infer general practical guidelines for lighting applications, and it becomes urgent to establish accurate and shared analysis protocols to be applied case by case, to study the effects of the environmental conditions on microorganisms and drive-specific design choices.

Preferred method and performance evaluation of heterogeneous composite phase change material (CPCM) wallboard in different seasons

The application of phase change materials (PCMs) in building envelopes has been developed in past decades and many strategies have been adopted to improve the heat resistance and capacity performance. However, rare studies focused on optimizing these characteristics of PCM wallboard in different seasons and providing preferred method for selecting the optimal PCMs. This study constructed a heterogeneous wall acting in different seasons and developed a pre-screening method of PCMs based on the analytic hierarchy process. CA-HD/EG with higher thermal conductivity (2.16 W/(m·K)) and lower melting point (25.4 °C) acted as the inner layer, and paraffin/porous silica with lower thermal conductivity (0.37 W/(m·K)) and higher melting point (29.3 °C) acted as the outer layer. Two experimental boxes were built to compare the thermal performance of CPCM and gypsum wallboard. The decrement factor in winter increased by 6.7 %, and the heat storage coefficient in summer increased by 0.97. The thermal comfort duration in winter was extended by 17.9 %, and the thermal load leveling in summer was reduced by 11.5 %. The heating/cooling water supply duration was shortened by 2.5 h, resulting in an energy-saving rate of 13.3 %. Consequently, the CPCM wallboard maintained a better indoor thermal environment with a lower energy consumption.

The importance of auditory, olfactory, and visual cues for insect foraging in owl monkeys (Aotus nancymaae).

Nocturnal mammals have unique sensory adaptations to facilitate foraging at night. Owl monkeys (Aotus spp.) are pair-living nocturnal platyrrhines adept at capturing insect prey under low-light conditions. Owl monkeys use acoustic and chemical cues in intraspecific communication and use olfaction to detect fruit as they forage. We conducted an experiment to determine which cues (auditory, olfactory, and visual) Aotus nancymaae rely upon when foraging for insects. We scored the behavior of 23 captive owl monkeys during a series of trials in which monkeys were provided sensory boxes with insect cues either present (experimental box) or absent (control box). Each cue was tested alone and in combination with all other cues (multimodal cues). We used generalized linear mixed models to determine which cues elicited the greatest behavioral response. Owl monkeys approached and spent more time near experimental boxes than control boxes. Male owl monkeys were quicker than their female partners to approach the sensory boxes, suggesting that males may be less neophobic than females. The owl monkeys exhibited behaviors associated with olfaction and foraging (e.g., sneezing, trilling) during trials with multimodal cues and when only olfactory cues were present. When only visual or auditory cues were present, owl monkeys exhibited fewer foraging-related behaviors. After approaching a sensory box, however, they often touched boxes containing visual cues. A. nancymaae may rely on olfactory cues at night to detect a food source from several meters away and then rely more on visual cues once they are closer to the food source. Their use of sensory cues during insect foraging differs from nocturnal strepsirrhines, possibly reflecting physiological constraints associated with phylogeny, given that owl monkeys evolved nocturnality secondarily from a more recent diurnal ancestor.

Thermal exchanges, physiological responses and productive performance of Guinea Fowl (Numidia meleagris) subjected to different air temperatures.

The objective of this research was to evaluate the thermal exchanges, physiological responses, productive performance and carcass yield of Guinea Fowl confined under thermoneutral conditions and under thermal stress. For the experiment, 96 animals were confined in 8 experimental boxes of 1 m2 of area, each, divided in equal numbers and placed inside two distinct climatic chambers, where the birds were distributed in a completely randomized design, with two treatments (air temperatures of 26 and 32°C, respectively). For the collection of physiological responses and carcass yield 16 birds were evaluated and for the collection of data on feed and water consumption and productive responses, 48 birds per treatment were evaluated. The environmental variables (air temperature (AT), air relative humidity and wind speed), temperature and humidity index (THI), heat exchanges, physiological responses (respiratory rate, surface temperature, cloacal temperature and eyeball temperature), feed (FC) and water (WC) consumption and production responses (weight gain, feed conversion index and carcass yield) of the birds were evaluated. With the elevation of the AT, it could be noticed that the THI went from a thermal comfort condition to an emergency condition, where the birds lost part of their feathers, increased all physiological responses evaluated, and consequently, reduced by 53.5% the amount of heat dissipated in the sensible form and increased by 82.7% the heat losses in the latent form, increasing also the WC. ATs of up to 32°C did not significantly affect the productive performance and carcass yield of the guinea fowl.

Recolonization and recovery of an Arctic benthic community subject to mine-tailings deposits

Deposition of large volumes of mine tailings takes place in several Norwegian fjords, but the impacts on marine ecosystems have received relatively little scientific attention. At a 40 + −year old tailing deposition area for a copper mine in the Arctic fjord Repparfjorden, we investigated both short-term colonization of mine tailings-contaminated sediments through a field experiment, and the present faunal state in the old deposit area. Copper-concentrations at the old deposition site were still high (up to 291 mg/kg dry weight (dw)), and exceeded the Norwegian environmental-quality threshold (84 mg/kg dw). Furthermore, copper was identified as a significant structuring factor for the fauna in the fjord, although faunal diversity was relatively high and the community not severely disturbed. In the colonization experiment, experimental boxes filled with defaunated sediment capped with mine tailings were subject to colonization for 15 months. Benthic macrofaunal communities were successfully established in all boxes, but the boxes with tailings showed lower species richness, abundance and biomass than the controls. Mine tailings continue to have local impacts on seafloor communities decades after deposition, and even low levels of metal-rich sediments can affect faunal recruitment. These results have implications for submarine deposition of mining waste and the impacts they have on coastal ecosystems.

Effect of Oregano Essential Oil Combined with Live and Killed Newcastle Disease Vaccines on Immune Response in Broilers Chicks in Erbil, Iraq: A Comparative Study.

This trial was conducted to evaluate the effects of oregano essential oil supplementation at the concentration of 50 mL/1000L of drinking water on immune response and growth performance in broiler chickens. A total of 390 one-day-old Rose chickens were housed together in six experimental boxes (n=65 each), including non-vaccinated groups and non-vaccinated oregano treated groups. Group A: vaccinated with LaSota vaccine via eye drop at 5-, 15-, and 28-day-old adding oregano essential oil in drinking water 1 day post-vaccination. Group B: vaccinated with LaSota vaccine via eye drop at 5-, 15-, 28-day-old only without oregano essential oil. Group C: vaccinated with inactivated Newcastle disease (ND) vaccine at 5-day-old via subcutaneous (SC) injection one dose adding oregano essential oil in drinking water 1 day post-vaccination for 3 days. Group D: vaccinated with inactivated ND vaccine at 5-day-old via SC injection one dose only without oregano essential oil. Group E: non-vaccinated as the control group. Group F: non-vaccinated and adding oregano essential oil for 3 days in drinking water as the control group. The effect of oregano essential oil in drinking water combined with two forms of live and killed ND virus vaccines on the immune responses and serum proteins was investigated in broiler chickens. The results showed the significant effect of oregano on the immune response of chicks in groups D and C that were vaccinated with the killed vaccine at 23- and 33-day-old. Based on the findings, a peak was recorded at 38-day-old with a significant difference (P<0.05) in vaccinated and non-vaccinated groups, as well as a significant increase in 38-day-old for group C (P<0.05), compared to other groups regarding the oregano effects on the immune response of groups. In addition, an increase in the serum protein was significantly different (P0.05) in the vaccinated broiler chicks, and serum globulin showed a quantitative increase in the vaccinated broiler chicks, compared with non-vaccinated groups. However, the results showed that the effect of oregano was non-significantly different (P>0.05) and it had no beneficial effect on growth performance. The results of this study showed the effects of commercial Oregano supplementation (50 mL/1000L drinking water), which increased serum total protein, including globulin, and provoked the best immunological response to ND vaccinations in broiler chickens. More research is needed to develop a state that may beneficially affect the health and uniformity of chickens.

Productive and hematologic responses of country poultry subjected to different housing densities and water salinity levels

The aim of this research was to evaluate the production and hematologic responses of confined Rhode Island Red chickens consuming water with 3 different levels of salinity and housed at different densities. Seven hundred and twenty birds were distributed in 36 experimental boxes built inside a poultry house according to a completely randomized design with a 3 × 3 factorial scheme with 3 salinity levels (SL) of water (1, 4, and 8 dS/m) and 3 housing densities (8, 10, and 12 birds/m2). Four birds were evaluated from each experimental box, thus totaling 16 repetitions (birds) per treatment. The productive performance, carcass yield, and hematologic traits of the birds in different experimental conditions were evaluated. Increasing water SL resulted in a significant increase (P < 0.05) in water and feed consumption beginning in the sixth week of life, causing an increase in the percentage of carcass and heart weight, with no changes in serum responses. Increasing housing density led to a reduction in water and feed consumption, weight gain, and feed conversion, thus reducing the chickens' blood magnesium levels.

A plastic device fixed around trees can deter snakes from predating bird nest boxes

Several devices have been designed to prevent predation in nest boxes by mammals and birds. Although snakes are one of the most common predators in cavity–nesters, they have always been difficult to deter. Here we tested a method originally designed to avoid predation by tree–climbing mammals. To prevent snakes from climbing trees and predating on nest boxes, we wrapped a transparent acetate sheet of 80 cm high around tree trunks below a sample of 40 nest boxes used by tits. The acetate sheets were secured with duct tape. The remaining nest boxes (N = 74) in the study area were left as controls. The predation rate in the experimental nest boxes was 20 % and 2 % in control boxes. This method can be useful to increase bird breeding success, improving both the effectiveness of resources to obtain scientific data and the breeding success of endangered species.

A plastic device fixed around trees can deter snakes from predating bird nest boxes

Several devices have been designed to prevent predation in nest boxes by mammals and birds. Although snakes are one of the most common predators in cavity–nesters, they have always been difficult to deter. Here we tested a method originally designed to avoid predation by tree–climbing mammals. To prevent snakes from climbing trees and predating on nest boxes, we wrapped a transparent acetate sheet of 80 cm high around tree trunks below a sample of 40 nest boxes used by tits. The acetate sheets were secured with duct tape. The remaining nest boxes (N = 74) in the study area were left as controls. The predation rate in the experimental nest boxes was 20 % and 2 % in control boxes. This method can be useful to increase bird breeding success, improving both the effectiveness of resources to obtain scientific data and the breeding success of endangered species.

Description of the life cycle of Dolops discoidalis (Bouvier, 1899) (Branchiura: Argulidae), a parasite of the fish species Rhytiodus argenteofuscus (Kner, 1858) from the Brazilian Amazon.

The genus Dolops (Audouin, 1837) is endemic among ectoparasites present in fish, with nine species described in Brazil, five of which are from the Amazon region. Among the genus, the species Dolops discoidalis (Bouvier, 1899) stands out due to its high prevalence of parasitism in Amazonian fish. The present study aims to characterize the development phases of D. discoidalis. The parasites were obtained from infected fish species Rhytiodus argenteofuscus from a natural environment, kept in polyethylene experimental boxes (500 liters). The physical-chemical parameters of the water (pH, electrical conductivity, dissolved oxygen and temperature) were monitored daily. A total of 30 egg layings were analyzed. After identification of the oviposition, the eggs were transferred to another container containing water (0.5 liters). The number of eggs was recorded, with observations made in stereomicroscopic and photographic records until hatching occurred. The egg layings exhibited differences in colour throughout their development. On the seventh day, the embryos exhibited visible ocelli and on the 17th day the hatching period began. Hatching occurred in batches, with around 5–10 hatches/day/laying. However, peak hatching was recorded on the 19th day of the cycle. Therefore, the cycle of D. discoidalis comprised a total period of 17–22 days at 28.C, with hatching in batches and pigmentation of the eggs observed close to hatching. Newly hatched parasites did not survive for more than 24 hours in the absence of the host. This study contributes to knowledge of the biology of D. discoidalis, and adds to information regarding possible studies into its control.

Effect of dietary orange and grapefruit peel on growth performance, health status, meat quality and intestinal microflora of broiler chickens

In our experiment we studied the effect of dietary orange peel (OP) and grapefruit peel (GP) on broilers health and thigh meat quality. The experiment was conducted on 126 Cobb 500 broiler chicks, assigned to three dietary treatments with 42 broilers per group. Each treatment was conducted in 3 experimental growth boxes, corresponding to 6 replicate pens per treatment and 7 broilers per pen. The growth boxes had identical size with a capacity of 3.5 m2 per box. Experimental dietary treatments differed from control diet (C) by addition of 2% OP or 2% GP supplement. A significant increase in body weight was exhibited in OP group compared to GP group (p < .05). Serum energetic profile (glucose, cholesterol and triglyceride) were lowered in both OP and GP supplemented groups compared to C birds (p < .05). Among fatty acids in thigh meat, OP group has increased the sum of PUFA compared to GP group. Dietary OP and GP have effectively reduced the oxidation process occurring during storage measured by the thiobarbituric acid–reactive substances (TBARS) values in thigh meat. Therefore, lipid oxidation was delayed by incorporating the two citrus peels in broiler diet. Monitoring the antimicrobial effect of OP and GP on intestinal and caecal microbiota population, the supplements reduced the growth of pathogenic Escherichia coli and Staphylococcus spp., proving their antimicrobial effect, while the beneficial bacteria, Lactobacillus spp. was significantly improved. Highlights The use of vegetable residues in the animal diets is an important economic and environmental factor. The positive effects were observed on thigh meat lipid oxidation, serum energetic profile and intestinal microflora of broiler chickens. Citrus peel in broilers feeding can act as natural antioxidant and feed additive.

Macrofaunal colonization of mine tailings impacted sediments

An experiment was conducted to study and compare macrofaunal colonization of thin layers of mine tailings. Experimental boxes filled with marine sediments capped with mine tailings were placed on the seabed and subject to colonization for six and twelve months. Three Norwegian mine tailings, representative of major production processes, were used. In addition, one set of boxes served as control and was not treated with tailings. The layer thickness of the tailings was supposed to represent the thickness in the transition zone between the sea deposit itself and unaffected sediments. The most fine-grained tailings, which also contained flotation chemicals, showed a significantly lower colonization than the control and the other treatments. At the same time, all sediments were successfully colonized and rich in species. In general, the abundance of annelids was lower, while the abundance of mollusks was higher in the tailings-treatments than the controls. There were larger differences in faunal densities between the controls and tailings-treatments after six than twelve months, probably due to coverage by natural sedimentation and mixing of the thin tailings layer with the sediment underneath throughout the experiment. As the tailings initiated varying degree of effects on the benthos, there is expected to be a difference in how far the effects will extend outside the sea deposit. This is the first study where the colonization potential is systematically compared between various tailings, and as colonization is assumed a frequent and important mechanism for faunal restitution after disturbance events, the results are important for the management of tailings placements as well as with regard to other forms of disturbances associated with defaunated areas, like dredging and disposal of contaminated sediments.

PRODUCTION PERFORMANCE OF TAMBAQUI JUVENILES SUBJECTED TO SHORT FEED-DEPRIVATION AND REFEEDING CYCLES

This study proposes to investigate whether short restricted-feeding cycles during the pre-fattening phase of tambaqui (Colossoma macropomum) juveniles affect their production performance and yield in a semi intensive system and if this practice can be introduced as an alternative to reduce production costs in the pre-fattening phase of this species. The fish (8.07 ± 0.07 g) were subjected to short restricted-feeding periods under controlled laboratory conditions (experimental boxes) for 60 days (Phase I). Three feeding strategies were tested, namely: dairy feeding (Control); five days of feeding followed by two days of deprivation (5F/2D); and two days of feeding followed by four days of deprivation (2F/4D). Subsequently, the fish from each treatment (feeding regimes) evaluated in Phase 1 were transferred to an excavated pond with low water exchange and no supplemental aeration (semi-intensive system) where they were fed continuously for 65 days (Phase II). At the end of both phases, performance, metabolic and hematological parameters and the centesimal composition of the filets were analyzed and an economic assessment was undertaken based on the total operating cost (TOC) of production in the different feeding regimes. The restricted-feeding cycles 2F/4D and 5F/2D (Phase I) affected the performance of the fish, which showed lower daily weight gains and specific growth rates (SGR), resulting in lower viscerosomatic index (VSI) and morphometric measurements. However, apparent feed conversion did not differ across the treatments. The feed-deprived fish used triglycerides as an energy source, maintaining their blood glucose levels close to those of the continuously fed group (control). When they started to be feed daily for 65 days (Phase II), the metabolic (triglycerides) and hematological parameters (hematocrit and hemoglobin) equaled those of control group. Nevertheless, their final weight and morphometric measurements were lower than those of non-restricted feed, indicating a partial compensatory growth in tambaqui juveniles. Short restricted-feeding cycles applied in the pre fattening phase for 60 days negatively affected performance and led to metabolic and hematological alterations in tambaqui (Colossoma macropomum) juveniles. Although these short restricted-feeding cycles resulted in less expenditure on labor and feeding, the fish under those conditions did not develop equally to those fed daily, culminating in less biomass produced during the fattening period in a semi-intensive system for 65 days. Ultimately, this led to a higher total operating cost per gram of produced fish, demonstrating the economic infeasibility of this practice for tambaqui juvenile production.

The activity of the prelimbic cortex in rats is enhanced during the cooperative acquisition of an instrumental learning task

The objective of this study was to identify the functional properties of the prefrontal cortex that allow animals to work together to obtain a mutual reward. We induced pairs of male rats to develop a cooperative behavior in two adjacent Skinner boxes divided by a metallic grille. The experimental boxes allowed the two rats to see and to smell each other and to have limited physical contact through the grille. Rats were progressively trained to climb onto two separate platforms (and stay there simultaneously for >0.5 s) to get food pellets for both. This set-up was compatible with the in vivo recording of local field potentials (LFPs) at the prelimbic (PrL) cortex throughout the task. A dominant delta/theta activity appeared mostly during the period in which rats were located on the platforms. Spectral powers were larger when rats had to stay together on the platforms than when they jumped individually onto them. When paired together, rats presented significant differences in the power of delta and low theta bands depending if they were leading or following the joint activity. PrL cortex encodes neural commands related to the individual and joint acquisition of an operant conditioning task by behaving rats.

Effects of aeration on the suspended matter from a tropical and eutrophic estuary

A comprehensive understanding of the complex biogeochemical interactions between organic matter and persistent contaminants in the suspended matter is vital for eco-efficient estuary recovery. However, little is known regarding aeration effects in suspended particulate aggregates. Therefore, this study aimed to investigate the effects of aeration on the suspended matter from a Tropical and Eutrophic estuarine environment. Anoxic water with 60 g/L of suspended particulate matter (SPM) was collected from Guanabara Bay, Rio de Janeiro, Brazil, transferred to experimental boxes and aerated for 61 days. SPM aggregates monitoring included abiotic variables measurements and, determination of total organic matter (TOM), biopolymers composition, bacterial activity, trace metals, and polycyclic aromatic hydrocarbons (PAHs) concentrations. The aeration enhanced dissolved oxygen (DO) concentration and the redox potential (Eh). However, from days 0 to 61 the predominant bacterial activities were denitrification and fermentation. Electron transport system activity increased after day 10, and aerobic activity was detected after day 19. In summary, aeration increased aerobic bacterial activity, lipids (LIP) and trace metal concentrations, although diminished protein/carbohydrate ratio and PAH concentration. Trace metals concentration (Ni, Pb, Cu, Cr, Mn, and Fe) were the highest on day 19 when the pH was 5.9. Copper presented toxic values (Cu > 20.0 μg/g). The pH showed a strong negative correlation with Eh (r = −0.94; p < 0.001). Acidic environment (pH ≤ 5.9) in marine ecosystems with high loads of toxic trace metals is unsafe for biota. Therefore, managers must be aware of the environmental and biological risks of introducing the aeration technique into a eutrophic marine environment.

Investigating the potential of an autodissemination system for managing populations of vine weevil, Otiorhynchus sulcatus (Coleoptera: Curculionidae) with entomopathogenic fungi.

Vine weevil, also known as black vine weevil, (Otiorhynchus sulcatus) is an economically important pest affecting soft fruit and nursery stock in temperate regions. We used laboratory and polytunnel experiments to investigate a novel control system based on autodissemination of spores of an entomopathogenic fungus to populations of adult vine weevils. The fungus was applied as a conidial powder, used on its own or formulated with talc, to a simple plastic refuge for vine weevils. The potential for adult weevils to disseminate the fungus was investigated first in polytunnel experiments using fluorescent powders applied to the refuge in lieu of fungal conidia. In this system, 88% of adult weevils came in contact with the powder within 48 h. When the powder was applied to five adult weevils that were then placed within a population of 35 potential recipients, it was transmitted on average to 75% of the recipient population within 7 days. Three isolates of entomopathogenic fungi (Beauveria bassiana isolate codes 433.99 and 1749.11 and Metarhizium brunneum isolate code 275.86), selected from a laboratory virulence screen. These three isolates were then investigated for efficacy when applied as conidial powders in artificial refuges placed among populations of adult weevils held in experimental boxes in the laboratory at 20 °C. Under this regime, the fungal isolates caused 70-90% mortality of adult weevils over 28 days. A final polytunnel experiment tested the efficacy of conidial powders of M. brunneum 275.86 placed in artificial refuges to increase vine weevil mortality. Overall weevil mortality was relatively low (26-41%) but was significantly higher in cages in which the conidial powders were placed in refuge traps than in cages with control traps. The lower weevil mortality recorded in the polytunnel experiment compared to the laboratory test was most likely a consequence of the greater amounts of inoculum required to kill adult weevils when conditions fluctuate between favourable and unfavourable temperatures e.g. below 15 °C. The potential of an autodissemination system for entomopathogenic fungi as a means of controlling vine weevil as part of an integrated pest management programme is discussed.

Ahorro energético residencial en ciudades de zonas áridas

La adopción de estructuras vegetadas no tradicionales en ciudades de zonas áridas, -nuevas tecnologías como cubiertas y muros verdes- constituye estrategias que permiten el control de temperaturas interiores extremas durante el verano, que a escala urbana favorecen la mitigación del fenómeno Isla de Calor Urbana. Este trabajo analiza la incidencia de dos especies incorporadas a cubiertas vegetadas y adaptadas a condiciones de déficit hídrico -Aptenia cordifolia y Sedum spectabile-, sobre la eficiencia térmica de espacios interiores. El desempeño térmico ha sido monitoreado durante verano e invierno en box experimentales. Los resultados muestran que las cubiertas vegetadas reducen la temperatura interior 1,6°C en verano con un consecuente ahorro energético para refrigeración del 30-35%. En invierno dicho ahorro es del 2-4% en horario diurno. Estos resultados indican la importancia de evaluar nuevas tecnologías verdes en términos de diseños adaptados localmente, de modo de maximizar su eficiencia energética y garantizar la sustentabilidad urbano-edilicio.

Usage of probiotic in country-type broiler's chicken diet and its effects over the carcass yield, morphology and the gastrointestinal pH

SUMMARY This study had as an objective to evaluate the carcass yield, morphology and intestinal pH of male country hick broilers raised on Tifton-85 pickets, fed with commercial probiotic supplemented ration. A total of 210 one-day-old males broiler's chicken from the Peeled Neck lineage were raised in experimental boxes on a conventional shed until 30 days of age, and from 30 to 90 days of age they had access to Tifton-85 pickets (Cynodonspp.). The carcass yield variables evaluated were live weight at 90 days, carcass weight (with foot, neck and head), carcass weight (without foot, neck and head), thigh, chest, wings, edible viscera, inedible viscera, abdominal fat, pH, length and weight of the gastrointestinal tract compartments (gut, duodenum, jejunum, ileus, proventriculus, gizzard, and cecum). All data went through analysis of variance, and the means of the treatments were compared by the F test with 5% of probability. Variables of carcass yield, primary and secondary cuts, length, weight and pH of gastrointestinal tract compartments were not influenced (P&gt; 0.05) by treatments with and without probiotic addition. Inclusion of 300 g / ton of the probiotic Bacillus subtilis DSM 15,544 in broiler's chicken from the Peeled Neck lineage had no influence over the carcass yield, pH and morphology of the gastrointestinal compartments of the birds in the period and levels in the present experiment. The sanitary challenge was not enough to demonstrate the effectiveness of probiotic action.

Design of Innovative Digital Electronic Technology Experimental Case Teaching

Due to the fact that the traditional Digital Electronic Technology (hereinafter referred to as DET) experiments bear sufficient reference material, elaborate design plan and complete design circuits (which makes it difficult for the students to design some experiments by themselves innovatively), but fail to bear enough experimenting methods, combined with the requirements raised by the competitions for experiment case teaching of elementary curriculums on electronic engineering and technique, in order to make some changes to the traditional experiment teaching, the paper innovated a new experiment case for the design of Rock-paper-scissors Game. In the design, two methods, Digital-integrated-circuit method and programmable-techniques method, are adopted to make it come true. When adopting digital-integrated-circuit method, right integrated circuit components will be employed according to the game-designing plan, using the software of Proteus to simulate it first, followed by debugging and testing of the hardware circuits on the bread board. The programmable-techniques method can describe the functions of the game on the development platform of the software of Quartus II by programming, adopting HDL (hardware description language). After right simulation, the programs will be downloaded onto FPGA experimental boxes or development board to realize the design of the game. The experimental case of Rock-paper-scissors Simulation Game is interesting and novel, which tends to stimulate the students to explore new world, study and innovate new things on their own. The adoption of the two methods will not only further enhance students’ knowledge application ability and engineer practice ability, but also help them better understand the difference of an experiment between the traditional and modern method, which will, in turn, understand the significance and value of programmable techniques in digital system designing.

Design of Innovative Digital Electronic Technology Experimental Case Teaching

Due to the fact that the traditional Digital Electronic Technology (hereinafter referred to as DET) experiments bear sufficient reference material, elaborate design plan and complete design circuits (which makes it difficult for the students to design some experiments by themselves innovatively), but fail to bear enough experimenting methods, combined with the requirements raised by the competitions for experiment case teaching of elementary curriculums on electronic engineering and technique, in order to make some changes to the traditional experiment teaching, the paper innovated a new experiment case for the design of Rock-paper-scissors Game. In the design, two methods, Digital-integrated-circuit method and programmable-techniques method, are adopted to make it come true. When adopting digital-integrated-circuit method, right integrated circuit components will be employed according to the game-designing plan, using the software of Proteus to simulate it first, followed by debugging and testing of the hardware circuits on the bread board. The programmable-techniques method can describe the functions of the game on the development platform of the software of Quartus II by programming, adopting HDL (hardware description language). After right simulation, the programs will be downloaded onto FPGA experimental boxes or development board to realize the design of the game. The experimental case of Rock-paper-scissors Simulation Game is interesting and novel, which tends to stimulate the students to explore new world, study and innovate new things on their own. The adoption of the two methods will not only further enhance students’ knowledge application ability and engineer practice ability, but also help them better understand the difference of an experiment between the traditional and modern method, which will, in turn, understand the significance and value of programmable techniques in digital system designing.