ABSTRACT The current study examined associations between stressors in the work-family system and negative affect among three ethnic communities in Israeli society. The total sample included 817 participants (65% women) consisting of 269 modern Jews, 147 ultra-Orthodox Jews and 269 Israeli Arabs. Participants completed measures of objective load in the family and work, role conflict, and negative affect. Work-family role conflict emerged as a significant mediator between objective load (experienced at work) and negative affect. Similarities and differences were found across ethnic communities and genders. The association between experiencing role conflict and negative affect is stronger among members of the modern Jewish community than among traditional ones (ultra-Orthodox Jews and Arabs). The research findings present a multifaceted picture regarding the relationship between stressors and their adverse impact on mental well-being across various ethnic communities. While some consistent patterns emerged, indicating a uniform impact of certain stressors, other findings suggest that the association between stress experienced within the family-work system and mental distress is heavily dependent on culture. Professionals are advised to develop culturally sensitive intervention programs that consider specific ethnic community cultural values at workplaces to alleviate tension in the work-family system.