Expressways use effective roadway designs to cut down on travel time and provide a far more pleasant experience, providing access to locations that was previously impossible. The Agra-Lucknow Expressway is the ideal illustration of an expressway that significantly benefited the tourist sectors of the towns of Agra and Lucknow. Due to this, local companies in these cities as well as those in the tourism sector have been able to gain from an increase in tourists from adjacent cities. In addition, several tourism-related enterprises grew in both cities as a result of the Agra-Lucknow Expressway's improved accessibility. The Agra-Lucknow Expressway has been a boon to the tourist industries of both Agra and Lucknow. All these aspects make it imperative to assess the impact of Agra- Lucknow expressway on tourism industry in Agra and Lucknow city. The present research is quantitative research wherein data has been collected via survey strategy. For testing the hypothesis Multivariate analysis has been conducted. SPSS 23.0 has been used for conducting the statistical analysis. The present research also found that there is a significant impact of Agra Lucknow Expressway on tourism industry in Agra and Lucknow city.