Excitation Gradient Research Articles

Immature Status Epilepticus Alters the Temporal Relationship between Hippocampal Interictal Epileptiform Discharges and High-frequency Oscillations

The aim was to investigate the long-term effects of a single episode of immature Status Epilepticus (SE) on the excitability of the septal and temporal hippocampus in vitro, by studying the relationship between interictal-like epileptiform discharges (IEDs) and high-frequency oscillations (HFOs; Ripples, Rs and Fast Ripples, FRs). A pentylenetetrazol-induced Status Epilepticus-(SE)-like generalized seizure was induced at postnatal day 20 in 22 male and female juvenile rats, sacrificed >40 days later to prepare hippocampal slices. Spontaneous IEDs induced by Mg2+-free ACSF were recorded from the CA3 area of temporal (T) or septal (S) slices. Recordings were band-pass filtered off-line revealing Rs and FRs and a series of measurements were conducted, with mean values compared with those obtained from age-matched controls (CTRs). In CTR S (vs T) slices, we recorded longer R & FR durations, a longer HFO-IED temporal overlap, higher FR peak power and more frequent FR initiation preceding IEDs (% events). Post-SE, in T slices all types of events duration (IED, R, FR) and the time lag between their onsets (R-IED, FR-IED, R-FR) increased, while FR/R peak power decreased; in S slices, the IED 1st population spike and the FR amplitudes, the R and FR peak power and the (percent) events where Rs or FRs preceded IEDs all decreased. The CA3 IED-HFO relationship offers insights to the septal-to-temporal synchronization patterns; its post-juvenile-SE changes indicate permanent modifications in the septotemporal excitability gradient. Moreover, these findings are in line to region-specific regulation of various currents post-SE, as reported in literature.

Rescue of Normal Excitability in LGI1-Deficient Epileptic Neurons.

Leucine-rich glioma inactivated 1 (LGI1) is a glycoprotein secreted by neurons, the deletion of which leads to autosomal dominant lateral temporal lobe epilepsy. We previously showed that LGI1 deficiency in a mouse model (i.e., knock-out for LGI1 or KO-Lgi1) decreased Kv1.1 channel density at the axon initial segment (AIS) and at presynaptic terminals, thus enhancing both intrinsic excitability and glutamate release. However, it is not known whether normal excitability can be restored in epileptic neurons. Here, we show that the selective expression of LGI1 in KO-Lgi1 neurons from mice of both sexes, using single-cell electroporation, reduces intrinsic excitability and restores both the Kv1.1-mediated D-type current and Kv1.1 channels at the AIS. In addition, we show that the homeostatic-like shortening of the AIS length observed in KO-Lgi1 neurons is prevented in neurons electroporated with the Lgi1 gene. Furthermore, we reveal a spatial gradient of intrinsic excitability that is centered on the electroporated neuron. We conclude that expression of LGI1 restores normal excitability through functional Kv1 channels at the AIS.SIGNIFICANCE STATEMENT The lack of leucine-rich glioma inactivated 1 (LGI1) protein induces severe epileptic seizures that leads to death. Enhanced intrinsic and synaptic excitation in KO-Lgi1 mice is because of the decrease in Kv1.1 channels in CA3 neurons. However, the conditions to restore normal excitability profile in epileptic neurons remain to be defined. We show here that the expression of LGI1 in KO-Lgi1 neurons in single neurons reduces intrinsic excitability, and restores both the Kv1.1-mediated D-type current and Kv1.1 channels at the axon initial segment (AIS). Furthermore, the homeostatic shortening of the AIS length observed in KO-Lgi1 neurons is prevented in neurons in which the Lgi1 gene has been rescued. We conclude that LGI1 constitutes a critical factor to restore normal excitability in epileptic neurons.

T-type Ca2+ and persistent Na+ currents synergistically elevate ventral, not dorsal, entorhinal cortical stellate cell excitability

Dorsal and ventral medial entorhinal cortex (mEC) regions have distinct neural network firing patterns to differentially support functions such as spatial memory. Accordingly, mEC layer II dorsal stellate neurons are less excitable than ventral neurons. This is partly because the densities of inhibitory conductances are higher in dorsal than ventral neurons. Here, we report that T-type Ca2+ currents increase 3-fold along the dorsal-ventral axis in mEC layer II stellate neurons, with twice as much CaV3.2 mRNA in ventral mEC compared with dorsal mEC. Long depolarizing stimuli trigger T-type Ca2+ currents, which interact with persistent Na+ currents to elevate the membrane voltage and spike firing in ventral, not dorsal, neurons. T-type Ca2+ currents themselves prolong excitatory postsynaptic potentials (EPSPs) to enhance their summation and spike coupling in ventral neurons only. These findings indicate that T-type Ca2+ currents critically influence the dorsal-ventral mEC stellate neuron excitability gradient and, thereby, mEC dorsal-ventral circuit activity.

Conduction of excitation waves and reentry drift on cardiac tissue with simulated photocontrol-varied excitability.

The development of new approaches to suppressing cardiac arrhythmias requires a deep understanding of spiral wave dynamics. The study of spiral waves is possible in model systems, for example, in a monolayer of cardiomyocytes. A promising way to control cardiac excitability in vitro is the noninvasive photocontrol of cell excitability mediated by light-sensitive azobenzene derivatives, such as azobenzene trimethylammonium bromide (AzoTAB). The trans-isomer of AzoTAB suppresses spontaneous activity and excitation propagation speed, whereas the cis isomer has no detectable effect on the electrical properties of cardiomyocyte monolayers; cis isomerization occurs under the action of near ultraviolet (UV) light, and reverse isomerization occurs when exposed to blue light. Thus, AzoTAB makes it possible to create patterns of excitability in conductive tissue. Here, we investigate the effect of a simulated excitability gradient in cardiac cell culture on the behavior and termination of reentry waves. Experimental data indicate a displacement of the reentry wave, predominantly in the direction of lower excitability. However, both shifts in the direction of higher excitability and shift absence were also observed. To explain this effect, we reproduced these experiments in a computer model. Computer simulations showed that the explanation of the mechanism of observed drift to a lower excitability area requires not only a change in excitability coefficients (ion currents) but also a change in the diffusion coefficient; this may be the effect of the substance on intercellular connections. In addition, it was found that the drift direction depended on the observation time due to the meandering of the spiral wave. Thus, we experimentally proved the possibility of noninvasive photocontrol and termination of spiral waves with a mechanistic explanation in computer models.

Prenatal alcohol exposure affects developmental differentiation of interictal discharges in septal and temporal hippocampus.

Prenatal alcohol exposure (PAE) provokes lifelong CNS dysfunction, including an increased susceptibility to seizure disorders. We investigated hippocampal excitability in vitro in the offspring of dams exposed to a mild ethanol concentration throughout pregnancy (ethanol 15%v/v in drinking water). Hippocampal slices were prepared from the offspring at a young (Y, 21-30 postnatal days, PND) or adult (A, 60 PND) age, with controls from same age normal rats (N). Synchronous spontaneous interictal-type epileptiform discharges (IEDs) were induced by bathing the slices in Mg2+-free ACSF or in 4-Aminopyridine (4-AP, 50µΜ) and were recorded from CA1 pyramidal layer of temporal (T) and septal slices (S). Hippocampal slices readily generated IEDs following NMDA receptor activation or K+ conductance block, with frequency and duration depending on location (septal or temporal), age, the activating mechanism, and prior conditioning (N or PAE). From the two media, 4-AP induced higher frequency (always), shorter duration (mostly) IEDs compared to Mg 2+-free ACSF. Temporal IED frequency increased with age, whereas septal was stable, indicating an earlier maturation of the latter part. The hippocampal "T to S" (high to low) excitability gradient appeared at/later than the end of the first postnatal month and mostly concerned discharge frequency. Discharge duration generally decreased with maturation but appeared to depend on many factors, including conditioning. Prenatal alcohol exposure differentiated the control of synchronous discharges by NMDA receptors and K+ conductances, and their developmental evolution, thus suggesting potential mechanisms for aberrant hippocampal neuronal network function.

Highly efficient implementation of the analytical gradients of pseudospectral time-dependent density functional theory.

The accuracy and efficiency of time-dependent density functional theory (TDDFT) excited state gradient calculations using the pseudospectral method are presented. TDDFT excited state geometry optimizations of the G2 test set molecules, the organic fluorophores with large Stokes shifts, and the Pt-complexes show that the pseudospectral method gives average errors of 0.01-0.1 kcal/mol for the TDDFT excited state energy, 0.02-0.06pm for the bond length, and 0.02-0.12° for the bond angle when compared to the results from conventional TDDFT. TDDFT gradient calculations of fullerenes (Cn, n up to 540) with the B3LYP functional and 6-31G** basis set show that the pseudospectral method provides 8- to 14-fold speedups in the total wall clock time over the conventional methods. The pseudospectral TDDFT gradient calculations with a diffuse basis set give higher speedups than the calculations for the same basis set without diffuse functions included.

The peculiar nebula Simeis 57

Simeis 57 (HS 191) is an optically bright nebula in the Cygnus X region with a peculiar appearance that suggests an outflow from a rotating source. Newly obtained observations and archival data reveal Simeis 57 as a low-density (ne ~ 100 cm−3) nebula with an east-to-west excitation gradient. The extinction of the nebula is AV ≤ 2 mag. The nebula is recognizable but not prominent in mid- and far-infrared images. In its direction, half a dozen small CO clouds have been identified at VLSR = +5 km s−1. One of these coincides with both the optical nebula and a second CO cloud at the nebular velocity VLSR ≈−10 km−1. No luminous stars are embedded in these molecular clouds, nor are any obscured by them and no sufficiently luminous stars are found in the immediate vicinity of the nebula. Instead, all available data points to the evolved star HD 193793 = WR 140 (an O4-5 supergiant and WC7 Wolf–Rayet binary) as the source of excitation, notwithstanding its large separation of 50′, about 25 pc at the stellar distance of 1.7 kpc. Simeis 57 appears to be a part of a larger structure surrounding the HI void centered on HD 193793.

Monte Carlo Modeling of Shortwave-Infrared Fluorescence Photon Migration in Voxelized Media for the Detection of Breast Cancer.

Recent progress regarding shortwave-infrared (SWIR) molecular imaging technology has inspired another modality of noninvasive diagnosis for early breast cancer detection in which previous mammography or sonography would be compensated. Although a SWIR fluorescence image of a small breast cancer of several millimeters was obtained from experiments with small animals, detailed numerical analyses before clinical application were required, since various parameters such as size as well as body hair differed between humans and small experimental animals. In this study, the feasibility of SWIR was compared against visible (VIS) and near-infrared (NIR) region, using the Monte Carlo simulation in voxelized media. In this model, due to the implementation of the excitation gradient, fluorescence is based on rational mechanisms, whereas fluorescence within breast cancer is spatially proportional to excitation intensity. The fluence map of SWIR simulation with excitation gradient indicated signals near the upper surface of the cancer, and stronger than those of the NIR. Furthermore, there was a dependency on the fluence signal distribution on the contour of the breast tissue, as well as the internal structure, due to the implementation of digital anatomical data for the Visible Human Project. The fluorescence signal was observed to become weaker in all regions including the VIS, the NIR, and the SWIR region, when fluorescence-labeled cancer either became smaller or was embedded in a deeper area. However, fluorescence in SWIR alone from a cancer of 4 mm diameter was judged to be detectable at a depth of 1.4 cm.

ALMA resolves the remarkable molecular jet and rotating wind in the extremely radio-quiet galaxy NGC 1377

Submillimetre and millimetre line and continuum observations are important in probing the morphology, column density, and dynamics of the molecular gas and dust around obscured active galactic nuclei (AGNs) and their mechanical feedback. With very high-resolution (0.​​″02 × 0.​​″03 (2 × 3 pc)) ALMA 345 GHz observations of CO 3–2, HCO+ 4–3, vibrationally excited HCN 4–3 ν2 = 1f, and continuum we have studied the remarkable, extremely radio-quiet, molecular jet and wind of the lenticular galaxy NGC 1377. The outflow structure is resolved, revealing a 150 pc long, clumpy, high-velocity (∼600 km s−1), collimated molecular jet where the molecular emission is emerging from the spine of the jet with an average diameter of 3–7 pc. The jet widens to 10–15 pc about 25 pc from the centre, which is possibly due to jet-wind interactions. A narrow-angle (50°–70°), misaligned and rotating molecular wind surrounds the jet, and both are enveloped by a larger-scale CO-emitting structure at near-systemic velocity. The jet and narrow wind have steep radial gas excitation gradients and appear turbulent with high gas dispersion (σ > 40 km s−1). The jet shows velocity reversals that we propose are caused by precession, or more episodic directional changes. We discuss the mechanisms powering the outflow, and we find that an important process for the molecular jet and narrow wind is likely magneto-centrifugal driving. In contrast, the large-scale CO-envelope may be a slow wind, or cocoon that stems from jet-wind interactions. An asymmetric, nuclear r ∼ 2 pc dust structure with a high inferred molecular column density N(H2) ≃1.8 × 1024 cm−2 is detected in continuum and also shows compact emission from vibrationally excited HCN. The nuclear dust emission is hot (Td > 180 K) and its luminosity is likely powered by a buried AGN. The lopsided structure appears to be a warped disk, which is responsible for a significant part of the nuclear obscuration and possibly formed as a result of uneven gas inflows. The dynamical mass inside r = 1.4 pc is estimated to 9−3+2 × 106 M⊙, implying that the supermassive black hole (SMBH) has a high mass with respect to the stellar velocity dispersion of NGC 1377. We suggest that the SMBH of NGC 1377 is currently in a state of moderate growth, at the end of a more intense phase of accretion and also evolving from a state of more extreme nuclear obscuration. The nuclear growth may be fuelled by low-angular momentum gas inflowing from the gas ejected in the molecular jet and wind. Such a feedback-loop of cyclic outflows and central accretion could explain why there is still a significant reservoir of molecular gas in this ageing, lenticular galaxy. A feedback-loop would be an effective process in growing the nuclear SMBH and thus would constitute an important phase in the evolution of NGC 1377. This also invites new questions as to SMBH growth processes in obscured, dusty galaxies.

The 300-pc scale ALMA view of [C i] 3P1–3P0, CO J = 1–0, and 609-μm dust continuum in a luminous infrared galaxy

ABSTRACT We present high-quality Atacama Large Millimeter/submillimeter Array (ALMA) Band 8 observations of the [C i] 3P1–3P0 line and 609-μm dust continuum emission towards the nearby luminous infrared galaxy (LIRG) IRAS F18293-3413, as well as matched resolution (300-pc scale) Band 3 CO J = 1–0 data, which allow us to assess the use of the [C i] 3P1–3P0 line as a total gas mass estimator. We find that the [C i] line basically traces structures detected in CO (and dust) and a mean (median) [C i]/CO luminosity ($L^{\prime }_{\rm [C\, {\small I}]}$/$L^{\prime }_{\rm CO}$) ratio of 0.17 (0.16) with a scatter of 0.04. However, a pixel-by-pixel comparison revealed that there is a radial $L^{\prime }_{\rm [C\, {\small I}]}$/$L^{\prime }_{\rm CO}$ gradient and a superlinear $L^{\prime }_{\rm CO}$ versus $L^{\prime }_{\rm [C\, {\small I}]}$ relation (slope = 1.54 ± 0.02) at this spatial scale, which can be explained by radial excitation and/or line opacity gradients. Based on the molecular gas masses converted from the dust continuum emission, we found that the CO-to-H2 and [C i]-to-H2 conversion factors are relatively flat across the molecular gas disc with a median value of 3.5$^{+1.9}_{-1.3}$ and 20.7$^{+9.2}_{-4.9}$ M⊙ (K km s−1 pc2)−1, respectively. A non-LTE calculation yields that typical molecular gas properties seen in nearby (U)LIRGs ($n_{\rm H_2}$ = 103−4 cm−3, Tkin ∼ 50 K, and $X_{\rm C\, {\small I}}$ = (0.8–2.3) × 10−5) can naturally reproduce the derived [C i]-to-H2 conversion factor. However, we caution that a careful treatment of the physical gas properties is required in order to measure H2 gas mass distributions in galaxies using a single [C i] line. Otherwise, a single [C i] line is not a good molecular gas estimator in a spatially resolved manner.

Description method of Illumination invariant image features

Image feature extraction technology is one of main topics in the field of computer vision, which has been widely applied in biological recognition, image retrieval, target detection and other fields. To overcome the drawbacks of WLD under complex illumination condition, we propose a novel illumination–insensitive feature descriptor named as anisotropic Weber synergy gradient descriptor (AWSGD). The proposed algorithm contains two parts:differential excitation component and gradient direction component. Firstly, by introducing the differential synergy excitation pattern (DSEP) and anisotropic LOG operator with variable scales and angles, we propose the anisotropic differential synergy excitation pattern (ADSEP) as the differential excitation component. Next, focused on the shortage that local gradient pattern (LGP) lacks detailed description of local features with single-layer model, we propose weighted local synergy gradient pattern (WLSGP) as the gradient direction component based on two-layer structure model and weight coefficient distribution model. Finally, ADSEP and WLSGP are fused to form AWSGD histogram. Meanwhile, we adopt XGBoost classifier to conduct related experiments on face databases CMUPIE, Yale B and texture databases PhoTex, RawFooT. The experimental results indicate that the proposed algorithm has stronger robustness to illumination variation and achieves the best performance compared with state-of-the-art methods, which has a certain theoretical significance and practical value in image recognition field under complex illumination condition.

Macroscopic gradients of synaptic excitation and inhibition in the neocortex.

With advances in connectomics, transcriptome and neurophysiological technologies, the neuroscience of brain-wide neural circuits is poised to take off. Amajor challenge is to understand how a vast diversity of functions is subserved by parcellated areas of mammalian neocortex composed of repetitions of a canonical local circuit. Areas of the cerebral cortex differ from each other not only in their input-output patterns but also in their biological properties. Recent experimental and theoretical work has revealed that such variations are not random heterogeneities; rather, synaptic excitation and inhibition display systematic macroscopic gradients across the entire cortex, and they are abnormal in mental illness. Quantitative differences along these gradients can lead to qualitatively novel behaviours in non-linear neural dynamical systems, by virtue of a phenomenon mathematically described as bifurcation. The combination of macroscopic gradients and bifurcations, in tandem with biological evolution, development and plasticity, provides a generative mechanism for functional diversity among cortical areas, as a general principle of large-scale cortical organization.

Stopping waves: geometric analysis of coupled bursters in an asymmetric excitation field

Bursting is a type of electrical activity seen in many neurons and endocrine cells where episodes of action potential firing are interspersed by silent phases. Here, we investigate partial synchrony and wave propagation in a population of square-wave bursters. In particular, by using a prototypical polynomial bursting model and slow/fast bifurcation analysis, we study why electrically coupled model bursters typically synchronize very easily, as reflected in the tendency for simulated excitation waves to propagate far into the region of silent cells when an excitation gradient is imposed. Such simulation is inspired by, but does not reproduce, experimentally observed Ca2+ waves in pancreatic islets exposed to a glucose gradient. Our analyses indicate a possible modification of the model so that the excitation waves stop at the border between “active” and “silent” cells. We verify this property by simulations using such a modified model for a chain, and for a cubic cluster, of coupled cells. Furthermore, we show how our one- and two-parameter bifurcation analyses allow us to predict where the simulated waves stop, for both the original model and the modified version.

Epilepsy-Induced Reduction in HCN Channel Expression Contributes to an Increased Excitability in Dorsal, But Not Ventral, Hippocampal CA1 Neurons.

CA1 neurons in epileptic animals are vulnerable to selective changes in ion channel expression, called acquired channelopathies, which can increase the excitability of a neuron. Under normal conditions there is a gradient of ion channel expression and intrinsic excitability along the longitudinal, dorsoventral axis of hippocampal area CA1 of the rodent. Many of these channels, including M-channels, GIRK channels and HCN channels, all have dorsoventral expression gradients that might be altered in rodent models of epilepsy. Here, we show that the excitability of dorsal, but not ventral CA1 neurons, had an increased firing rate, reduced interspike interval (ISI) and increased input resistance in a status epilepticus (SE) model of temporal lobe epilepsy (TLE). As a result, the excitability of CA1 neurons became uniform across the dorsoventral axis of the rat hippocampus post-SE. Using current clamp recordings with pharmacology and immunohistochemistry, we demonstrate that the expression of HCN channels was downregulated in the dorsal CA1 region post-SE, while the expression of M and GIRK channels were unchanged. We did not find this acquired channelopathy in ventral CA1 neurons post-SE. Our results suggest that the excitability of dorsal CA1 neurons post-SE increase to resemble the intrinsic properties of ventral CA1 neurons, which likely makes the hippocampal circuit more permissible to seizures, and contributes to the cognitive impairments associated with chronic epilepsy.

Anatomo-functional characterisation of the human “hand-knob”: A direct electrophysiological study

The cortical area within the human primary motor cortex (M1) that hosts the representation of the hand and fingers is known as the ‘hand-knob’ and is essential for voluntary hand movement. The anatomo-functional heterogeneity described within the monkey primary motor cortex (M1) in a rostro-caudal direction suggests an internal subdivision in two sectors originating different systems of connections to the spinal cord. Direct investigation of the human hand-knob has been prevented, so far, by methodological constraints. The unique setting of brain tumour resection with the brain mapping technique in awake patients enables direct electrophysiological investigation of the functional properties of the human hand-knob. Motor-evoked potentials (MEPs) elicited by Direct Electrical Stimulation (DES) at high frequency (HF-DES) delivered along the hand-knob in rostro-caudal direction, i.e., from the central to the precentral sulcus, were recorded from the hand/arm muscles in patients at rest. The sites located near the precentral sulcus identified with HF-DES were then stimulated with low-frequency DES (LF-DES) during a hand manipulation task (HMt) to assess whether DES affected task execution. From the stimulated sites, corticofugal projections and U-shaped tracts connecting with adjacent gyri were traced using diffusion tensor and spherical deconvolution tractography. Analysis of MEPs showed a rostro-caudal gradient of cortical excitability along the hand-knob (the rostral sector being less excitable). Stimulation of rostral sites during the HMt impaired the task by inducing dysfunctional recruitment or, alternatively, suppression of distal muscles. Diffusion tractography showed different patterns of rostro-caudal connectivity for the U-shaped tracts. Overall data suggests, in humans, the anatomo-functional subdivision of the human hand-knob in two sectors, possibly subserving different roles in motor control.

Efficient calculation of (resonance) Raman spectra and excitation profiles with real-time propagation.

We investigate approaches for the calculation of (resonance) Raman spectra in a real-time time-dependent density functional theory (RT-TDDFT) framework. Several short time approximations to the Kramers, Heisenberg, and Dirac polarizability tensor are examined with regard to the calculation of resonance Raman spectra: One relies on a Placzek type expansion of the electronic polarizability and the other one relies on the excited state gradient method. The first one is shown to be in agreement with an approach based on perturbation theory in the case of a weak δ-pulse perturbation. The latter is newly applied in a real time propagation framework, enabled by the use of Padé approximants to the Fourier transform which allow for a sufficient resolution in the frequency domain. An analysis of the performance of Padé approximants is given. All approaches were found to be in good agreement for uracil and R-methyloxirane. Moreover it is shown how RT-TDDFT can be used to calculate Raman excitation profiles efficiently.

Zero-gradient-excitation ramped hybrid encoding (zGRF -RHE) sodium MRI.

Fast bi-exponential transverse signal decay compounds sodium image quality. This work aims at enhancing image characteristics using a special case of ramped hybrid encoding (RHE). Zero-gradient-excitation (zGRF )-RHE provides (1) gradient-free excitation for high flip angle, artifact-free excitation profiles and (2) gradient ramping during dead-time for the optimization of encoding time (tenc ) to reduce T2* signal decay influence during acquisition. Radial zGRF -RHE and standard radial UTE were investigated over a range of receiver bandwidths in simulations, phantom and in vivo brain experiments. Central k-space in zGRF -RHE was acquired through single point measurements at the minimum achievable TE. T2* blurring artifacts and image SNR and CNR were assessed. zGRF -RHE enabled 90° flip angle artifact-free excitation, whereas gradient pre-ramping provided greater tenc efficiency for any readout bandwidths. Experiments confirmed simulation results, revealing sharper edge characteristics particularly at short readout durations (TRO ). Significant SNR improvements of up to 4.8% were observed for longer TRO . zGRF -RHE allows for artifact-free high flip angle excitation with time-efficient encoding improving on image characteristics. This hybrid encoding concept with gradient pre-ramping is trajectory independent and can be introduced in any center-out UTE trajectory design.

MR-compatibility assessment of MADPET4: a study of interferences between an SiPM-based PET insert and a 7 T MRI system

Compromises in the design of a positron emission tomography (PET) insert for a magnetic resonance imaging (MRI) system should minimize the deterioration of image quality in both modalities, particularly when simultaneous demanding acquisitions are performed. In this work, the advantages of using individually read-out crystals with high-gain silicon photomultipliers (SiPMs) were studied with a small animal PET insert for a 7 T MRI system, in which the SiPM charge was transferred to outside the MRI scanner using coaxial cables. The interferences between the two systems were studied with three radio-frequency (RF) coil configurations. The effects of PET on the static magnetic field, flip angle distribution, RF noise, and image quality of various MRI sequences (gradient echo, spin echo, and echo planar imaging (EPI) at 1H frequency, and chemical shift imaging at 13C frequency) were investigated. The effects of fast-switching gradient fields and RF pulses on PET count rate were studied, while the PET insert and the readout electronics were not shielded. Operating the insert inside a 1H volume coil, used for RF transmission and reception, limited the MRI to T1-weighted imaging, due to coil detuning and RF attenuation, and resulted in significant PET count loss. Using a surface receive coil allowed all tested MR sequences to be used with the insert, with 45–59% signal-to-noise ratio (SNR) degradation, compared to without PET. With a 1H/13C volume coil inside the insert and shielded by a copper tube, the SNR degradation was limited to 23–30% with all tested sequences. The insert did not introduce any discernible distortions into images of two tested EPI sequences. Use of truncated sinc shaped RF excitation pulses and gradient field switching had negligible effects on PET count rate. However, PET count rate was substantially affected by high-power RF block pulses and temperature variations due to high gradient duty cycles.

Outflows in the narrow-line region of bright Seyfert galaxies – I. GMOS-IFU data

We present two-dimensional maps of emission-line fluxes and kinematics, as well as of the stellar kinematics of the central few kpc of five bright nearby Seyfert galaxies -- Mrk\,6, Mrk\,79, Mrk\,348, Mrk\,607 and Mrk\,1058 -- obtained from observations with the Gemini Multi-Object Spectrograph (GMOS) Integral Field Unit (IFU) on the Gemini North Telescope. The data cover the inner 3\farcs5$\times$5\farcs0 -- corresponding to physical scales in the range 0.6$\times$0.9 to 1.5$\times$2.2\,kpc$^2$ -- at a spatial resolution ranging from 110 to 280 pc with a spectral coverage of 4300 -- 7100\,\AA\ and velocity resolution of $\approx$ 90\,km\,s$^{-1}$. The gas excitation is Seyfert like everywhere but show excitation, but show excitation gradients that are correlated with the gas kinematics, reddening and/or the gas density. The gas kinematics show in all cases two components: a rotation one similar to that observed in the stellar velocity field, and an outflow component. In the case of Mrk607, the gas is counter-rotating relative to the stars. Enhanced gas velocity dispersion is observed in association to the outflows according to two patterns: at the locations of the highest outflow velocities along the ionization axis or perpendicularly to it in a strip centered at the nucleus that we attribute to an equatorial outflow. Bipolar outflows are observed in Mrk\,348 and Mrk\,79, while in Mrk\,1058 only the blueshifted part is clearly observed, while in the cases of Mrk\,6 and Mrk\,607 the geometry of the outflow needs further constraints from modeling to be presented in a forthcoming study, where the mass flow rate and powers will also be obtained.

Theoretical investigation of the weak interactions of rare gas atoms with silver clusters by resonance Raman spectroscopy modeling

AbstractThe interactions of rare gas atoms (Rg = Ar, Kr, and Xe) with small neutral and cationic silver clusters have been investigated by density functional methods and the effect of these weak interactions on the resonance Raman spectra of the complexes has been evaluated. The resonance Raman technique that depends on the properties of ground and excited state, seems deeply sensitive to the weak rare gas–metal cluster interactions, and the use of inert gases has been proven to be an excellent approach to recognize the ability of this technique to detect extremely weak interactions. In this work, for , and complexes the IR, normal and resonance Raman spectra have been calculated and the effect of rare gas–cluster stretching vibration ( ) on the pattern and the relative intensities of different spectra have been investigated. The resonance Raman spectra for the weakly interacted complexes (with the interaction energies less than −2.0 kcal/mol) exhibit the vibration with the detectable intensity that its intensity increases by going from Ag6–Ar to Ag6–Xe complex. Moreover, the resonance Raman spectra (based on the excited state gradient approximation) for high intensity nearly degenerate excited states, proved the effect of accumulation of the excited state charge density on the relative intensity of vibration.