Partner Exchange Research Articles

The Hog1 MAPK substrate governs Candida glabrata-epithelial cell adhesion via the histone H2A variant.

CgHog1, terminal kinase of the high-osmolarity glycerol signalling pathway, orchestrates cellular response to multiple external stimuli including surplus-environmental iron in the human fungal pathogen Candida glabrata (Cg). However, CgHog1 substrates remain unidentified. Here, we show that CgHog1 adversely affects Cg adherence to host stomach and kidney epithelial cells in vitro, but promotes Cg survival in the iron-rich gastrointestinal tract niche. Further, CgHog1 interactome and in vitro phosphorylation analysis revealed CgSub2 (putative RNA helicase) to be a CgHog1 substrate, with CgSub2 also governing iron homeostasis and host adhesion. CgSub2 positively regulated EPA1 (encodes a major adhesin) expression and host adherence via its interactor CgHtz1 (histone H2A variant). Notably, both CgHog1 and surplus environmental iron had a negative impact on CgSub2-CgHtz1 interaction, with CgHTZ1 or CgSUB2 deletion reversing the elevated adherence of Cghog1Δ to epithelial cells. Finally, the surplus-extracellular iron led to CgHog1 activation, increased CgSub2 phosphorylation, elevated CgSub2-CgHta (canonical histone H2A) interaction, and EPA1 transcriptional activation, thereby underscoring the iron-responsive, CgHog1-induced exchange of histone partners of CgSub2. Altogether, our work mechanistically defines how CgHog1 couples Epa1 adhesin expression with iron abundance, and point towards specific chromatin composition modification programs that probably aid fungal pathogens align their adherence to iron-rich (gut) and iron-poor (blood) host niches.

Vortex-dipole impingement with convex and concave boundaries

The vortex-dipole interactions with convex and concave boundaries in a two-dimensional domain are analyzed using the Bhatnagar–Gross–Krook (BGK) collision-based lattice Boltzmann method (LBM). The formation and detachment of the boundary layers at the flat wall creates new dipoles of non-identical vortices, following cyclic trajectories and exhibiting logarithmic variations in the production of maximum vorticity with Reynolds number (Re). The vortex-dipole interactions with concave boundaries produce a series of secondary dipoles, whose relative strength linearly decreases for the given Re in subsequent vortex-releasing events. Oblique interactions with cavity corners cause secondary dipoles to undergo head-on collisions at the domain center, influencing partner exchange and orthogonal propagation. Conversely, convex boundaries split the primary vortex-dipole upon impingement, intensifying the vorticity production and strain effects. Whereas, the larger ingestion of vorticity at the convex corners of the “T-shaped” cavity forms new dipoles, which deflect, rebound, and follow a parabolic trajectory for the vortex exchange. Normalized enstrophy, Ω(t), and palinstrophy, P(t), show distinct peaks during dipole interactions with boundary walls, influencing enhanced kinetic energy, E(t), decay. Moreover, the evolution of E(t) and Ω(t) satisfies the relation valid for no-slip boundaries. The collision behavior, vorticity production, and vortex rebound are functions of Re. The convex boundaries modify the scaling results of maximum Ω(t) and P(t) to higher exponent values than the dipole interactions with flat and concave boundaries.

Dynamics of activation in the voltage-sensing domain of Ciona intestinalis phosphatase Ci-VSP

The Ciona intestinalis voltage-sensing phosphatase (Ci-VSP) is a membrane protein containing a voltage-sensing domain (VSD) that is homologous to VSDs from voltage-gated ion channels responsible for cellular excitability. Previously published crystal structures of Ci-VSD in putative resting and active conformations suggested a helical-screw voltage sensing mechanism in which the S4 helix translocates and rotates to enable exchange of salt-bridge partners, but the microscopic details of the transition between the resting and active conformations remained unknown. Here, by combining extensive molecular dynamics simulations with a recently developed computational framework based on dynamical operators, we elucidate the microscopic mechanism of the resting-active transition at physiological membrane potential. Sparse regression reveals a small set of coordinates that distinguish intermediates that are hidden from electrophysiological measurements. The intermediates arise from a noncanonical helical-screw mechanism in which translocation, rotation, and side-chain movement of the S4 helix are only loosely coupled. These results provide insights into existing experimental and computational findings on voltage sensing and suggest ways of further probing its mechanism.

What “True Listening” Means in Terms of Acting? How to Train Actors Listening Truly?

This study delves into the concept of "true listening" in acting and its challenges, originating from the author's experiences in acting education. Feedback consistently noted issues with genuine listening, despite the author's belief in their attentive listening, leading to a perceived lack of engagement. The study explores the essence of "true listening," emphasizing its significance in Chinese acting education alongside "true seeing" and "true touching." It identifies two key aspects: external attention and responsive engagement to stimuli. The study uncovers phenomena like "arrogant listening" where actors memorize lines and anticipate responses, sidelining actual listening, and "inferior listening" driven by line memorization anxiety. Additionally, actors' tendency to pre-plan improvisation, termed "pre-set listening," hinders responsiveness. The notion of "isolated listening" parallels modern detachment from information. To address these issues, the study recommends utilizing Stanislavsky's "system" and the Meisner technique. Stanislavsky's "creative state" concept fosters genuine listening, while exercises encourage authentic partner exchanges. The Meisner technique cultivates present-moment engagement through truth-sharing exercises. The study concludes with suggestions for Chinese acting instructors, aiming to enhance acting education by illuminating "true listening" and strategies to overcome challenges.

BAY58-2667 Activates Different Soluble Guanylyl Cyclase Species by Distinct Mechanisms that Indicate Its Principal Target in Cells is the Heme-Free Soluble Guanylyl Cyclase-Heat Shock Protein 90 Complex.

Nitric oxide (NO)-unresponsive forms of soluble guanylyl cyclase (sGC) exist naturally and in disease can disable NO-sGC-cGMP signaling. Agonists like BAY58-2667 (BAY58) target these sGC forms, but their mechanisms of action in living cells are unclear. We studied rat lung fibroblast-6 cells and human airway smooth muscle cells that naturally express sGC and HEK293 cells that we transfected to express sGC and variants. Cells were cultured to build up different forms of sGC, and we used fluorescence and FRET-based measures to monitor BAY58-driven cGMP production and any protein partner exchange or heme loss events that may occur for each sGC species. We found that: (i) BAY58 activated cGMP production by the apo-sGCβ-Hsp90 species after a 5-8 minute delay that was associated with apo-sGCβ exchanging its Hsp90 partner with an sGCα subunit. (ii) In cells containing an artificially constructed heme-free sGC heterodimer, BAY58 initiated an immediate and three times faster cGMP production. However, this behavior was not observed in cells expressing native sGC under any condition. (iii) BAY58 activated cGMP production by ferric heme sGC only after a 30-minute delay, coincident with it initiating a delayed, slow ferric heme loss from sGCβ We conclude that the kinetics favor BAY58 activation of the apo-sGCβ-Hsp90 species over the ferric heme sGC species in living cells. The protein partner exchange events driven by BAY58 account for the initial delay in cGMP production and also limit the speed of subsequent cGMP production in the cells. Our findings clarify how agonists like BAY58 may activate sGC in health and disease. SIGNIFICANCE STATEMENT: A class of agonists can activate cyclic guanosine monophosphate (cGMP) synthesis by forms of soluble guanylyl cyclase (sGC) that do not respond to NO and accumulate in disease, but the mechanisms of action are unclear. This study clarifies what forms of sGC exist in living cells, which of these can be activated by the agonists, and the mechanisms and kinetics by which each form is activated. This information may help to hasten deployment of these agonists for pharmaceutical intervention and clinical therapy.

Characteristics of Successful International Pharmacy Partnerships

Recommendations for global pharmacy collaborations are predominately derived from US institutions. This study utilized semi-structured interviews of global collaborators to assess important partnership components. Interviewees stated personal connections and understanding of each other's programs/systems were key components. Additionally, collaborators indicate that mutual benefits between partners can exist without the requirement for bidirectional exchange of learning experiences, and request and value partners and learners who are culturally aware, global citizens. This structured interview approach provided key insight into how to develop mutually beneficial, sustainable partnerships and provides additional confirmation that the five pillars of global engagement align with an international audience.

Late Mesolithic Individuals of the Danube Iron Gates Origin on the Dnipro River Rapids (Ukraine)? Archaeological and Bioarchaeological Records

Abstract In contrast to large-scale prehistoric migrations, associated with massive population shifts and changes in material culture, movements of small human groups or single individuals are barely visible but no less important. In publications of the 1960s–2000s, specificity of craniological, odontological, and metrical characteristics as well as stable isotope values of some individuals distinguishing the Late Mesolithic cemetery of Vasylivka II among other Mesolithic and Neolithic burial sites in the Dnipro River basin was explained by some gene flows. However, archaeologists could not develop these views since the original excavation report of 1953 and all grave goods from Vasylivka II were considered lost. Another old field document, where pendants of the pharyngeal teeth of fish, and the shells of spiral, probably Mediterranean, molluscs found there were mentioned, allowed the recent suggestion of the author of the current article that several individuals from the Danube Iron Gates region were interred in the cemetery. Previous arguments along with new evidence are presented here to develop this hypothesis. Re-found personal ornaments from one burial, the only available grave goods from Vasylivka II, are published here for the first time. The established regularity that most relatively young men and women from the graveyard have conditional “Danubian” δ13C values in the range from −20 to −21‰ assumes the mutual exchange of marriage partners born in the Iron Gates and the Dnipro Rapids. A waterborne route is discussed as a more probable mode of communication between these regions.

Meiosis in allopolyploid Arabidopsis suecica

SUMMARYPolyploidy is a major force shaping eukaryote evolution but poses challenges for meiotic chromosome segregation. As a result, first‐generation polyploids often suffer from more meiotic errors and lower fertility than established wild polyploid populations. How established polyploids adapt their meiotic behaviour to ensure genome stability and accurate chromosome segregation remains an active research question. We present here a cytological description of meiosis in the model allopolyploid species Arabidopsis suecica (2n = 4x = 26). In large part meiosis in A. suecica is diploid‐like, with normal synaptic progression and no evidence of synaptic partner exchanges. Some abnormalities were seen at low frequency, including univalents at metaphase I, anaphase bridges and aneuploidy at metaphase II; however, we saw no evidence of crossover formation occurring between non‐homologous chromosomes. The crossover number in A. suecica is similar to the combined number reported from its diploid parents Arabidopsis thaliana (2n = 2x = 10) and Arabidopsis arenosa (2n = 2x = 16), with an average of approximately 1.75 crossovers per chromosome pair. This contrasts with naturally evolved autotetraploid A. arenosa, where accurate chromosome segregation is achieved by restricting crossovers to approximately 1 per chromosome pair. Although an autotetraploid donor is hypothesized to have contributed the A. arenosa subgenome to A. suecica, A. suecica harbours diploid A. arenosa variants of key meiotic genes. These multiple lines of evidence suggest that meiosis in the recently evolved allopolyploid A. suecica is essentially diploid like, with meiotic adaptation following a very different trajectory to that described for autotetraploid A. arenosa.

Princezny z Hájku. K problematice elity ve střední době bronzové

The topic of this study is the possibilities of archaeological identification of the local elite in the Bronze Age. We‘re targeting the female elite, who are characterized by a particular form of costume. In a case study of the barrow cemetery of Šťáhlavy – Hájek, we are trying to show that the elite status of women in the Bronze Age was not based on their personal qualities, but rather on their affinity with an elite family. Their social status may therefore have been hereditary. On the other hand, it turns out that certain particularities of the costume (different types of necklaces, etc.) may point to different origins of women and thus the exchange of female partners within the wider elite community.

Post‐spillover effects of China's integration on Pakistan Stock Exchange

AbstractThe Pakistan Stock Exchange (PSX) strategic partnership with the Chinese consortium shifted trends and transition patterns from global to Chinese exchanges. Any substantial changes in these markets created a significant impact on the PSX. After the partnership with the Chinese consortium, Pakistan's mean returns and price volatility dynamics have shifted toward the regional exchanges, connectivity, and alliances, which has increased regional integration. Any systematic change in trading policies and framework of partner exchanges may substantially impact the trading dynamics and volume of the Pakistan stock market and local investors. This changing behavior will enhance financial synergies in the Pakistan stock market's trading capacity by bringing Chinese cross‐listed IPOs and portfolio investment and financial vulnerabilities in exchange rate risk and volatility spillover effects from partner exchanges.

US Surveillance of Foreign Currency Exchange and Macroeconomic Practices

AbstractThis article focuses on the manner in which the US identifies the exchange rate and macroeconomic policies of its key trading partners and the policy advice it gives to them, when according to the US set criteria the trading partner's exchange rate and macroeconomic policies contribute to the US trade deficit. The article focuses on the US apparatus that is set in two different pieces of US legislation concerned essentially with currency manipulation mainly from the perspective of the WTO and IMF legal regimes. The paper concludes that although the US regime concerns currency manipulation, and is implemented to address a compliance void under the IMF and WTO procedures, it is flawed and that the Reports prepared under the US legislation are not qualitatively enlightening.

HnRNP A1/A2 Proteins Assemble onto 7SK snRNA via Context Dependent Interactions

7SK small nuclear RNA (snRNA) is an abundant and ubiquitously expressed noncoding RNA that functions to modulate the activity of RNA Polymerase II (RNAPII) in part by stabilizing distinct pools of 7SK-protein complexes. Prevailing models suggest that the secondary structure of 7SK is dynamically remodeled within its alternative RNA-protein pools such that its architecture differentially regulates the exchange of cognate binding partners. The nuclear hnRNP A1/A2 proteins influence the biology of 7SK snRNA via processes that require an intact stem loop (SL) 3 domain; however, the molecular details by which hnRNPs assemble onto 7SK snRNA are yet to be described. Here, we have taken an integrated approach to present a detailed description of the 7SK-hnRNP A1 complex. We show that unbound 7SK snRNA adopts at least two major conformations in solution, with significant structural differences localizing to the SL2-3 linker and the base of SL3. Phylogenetic analysis indicates that this same region is the least genetically conserved feature of 7SK snRNA. By performing DMS modifications with the presence of excess protein, we reveal that hnRNP A1 binds with selectivity to SL3 through mechanisms that increase the flexibility of the RNA adjacent to putative binding sites. Calorimetric titrations further validate that hnRNP A1-SL3 assembly is complex with the affinity of discrete binding events modulated by the surrounding RNA structure. To interpret this context-dependent binding phenomenon, we determined a 3D model of SL3 to show that it folds to position minimal hnRNP A1/A2 binding sites (5′-Y/RAG-3′) within different local environments. SL3-protein complexes resolved by SEC-MALS-SAXS confirm that up to four hnRNP A1 proteins bind along the entire surface of SL3 via interactions that preserve the overall structural integrity of this domain. In sum, the collective results presented here reveal a specific role for a folded SL3 domain to scaffold hnRNP A1/A2-7SK assembly via mechanisms modulated by the surrounding RNA structure.

On the role of hydrogen-bond exchanges in the spectral diffusion of water

The dynamics of a vibrational frequency in a condensed phase environment, i.e., the spectral diffusion, has attracted considerable interest over the last two decades. A significant impetus has been the development of two-dimensional infrared (2D-IR) photon-echo spectroscopy that represents a direct experimental probe of spectral diffusion, as measured by the frequency-frequency time correlation function (FFCF). In isotopically dilute water, which is perhaps the most thoroughly studied system, the standard interpretation of the longest timescale observed in the FFCF is that it is associated with hydrogen-bond exchange dynamics. Here, we investigate this connection by detailed analysis of both the spectral diffusion timescales and their associated activation energies. The latter are obtained from the recently developed fluctuation theory for the dynamics approach. The results show that the longest timescale of spectral diffusion obtained by the typical analysis used cannot be directly associated with hydrogen-bond exchanges. The hydrogen-bond exchange time does appear in the decay of the water FFCF, but only as an additional, small-amplitude (<3%) timescale. The dominant contribution to the long-time spectral diffusion dynamics is considerably shorter than the hydrogen-bond exchange time and exhibits a significantly smaller activation energy. It thus arises from hydrogen-bond rearrangements, which occur in between successful hydrogen-bond partner exchanges, and particularly from hydrogen bonds that transiently break before returning to the same acceptor.

HIV Testing and Receiving Test Results by the Female Sex Workers; Retrospective Study using Data from Sri Lanka National HIV Prevention Programme

<strong>Introduction: </strong>Improving HIV testing among Key Populations (populations at high risk) is the first step to achieve 90:90:90 treatment targets. Studying the time taken to HIV testing and subsequent clinic visits to uptake the test results provide important inputs for development of HIV prevention programmes. <strong> </strong> <strong>Objectives: </strong>This study aims to understand the pattern of HIV testing behavior of Female Sex Workers (FSWs) over the time and provide recommendations for development of HIV prevention programmes for FSWs <strong> </strong> <strong>Methods: </strong>HIV testing data of 5,667 FSWs registered with national HIV prevention programme in 10 Districts of Sri Lanka during 2016 and 2017 were analyzed using, survival data analysis techniques and extended Prentice, Williams &amp; Peterson (PWP) gap time model. <strong> </strong> <strong>Results: </strong>Time taken to acceptance of HIV test is more dependent on individual factors of FSWs whereas uptake of test result is less dependent on individual factors. Youth FSWs take more time to accept an HIV test. FSWs who are operating in Districts out of Colombo, use condoms, reported undergone HIV testing before join the programme, take more time for HIV testing and uptake test results. FSWs with high partner exchange rate (therefore at high risk) are less likely to complete both events and take more time for testing. Interaction between FSW typology and condom use was found with the use of PWP model. <strong> </strong> <strong>Conclusion: </strong>High risk FSWs are more reluctant for HIV testing and uptake of test results. Factors identified in the study as associated with time taken for both events can be taken into account in planning future HIV preventions programs among FSWs.

A Firm’s Concentration of Vertical Value Chain Partner Exchanges and Effects on Innovation Returns

A firm’s investment in internal and extramural R&D activities is critical for its ability to innovate. However, ability to generate returns from its innovation investments might not be similar across firms. In this regard, a firm’s upstream and downstream value chain partners can influence its innovation preparedness by enriching it with knowledge about new components, technologies and market changes. But, these interactions may cause vulnerabilities to a firm such as knowledge spillovers and opportunistic behaviours, thereby constraining its ability to innovate and gain returns from innovation. We empirically address this issue in our study, and show how that there are detrimental effects when a firm has concentration of value chain partners and that they are contingent upon certain firm strategies and technological environment of the firm. We highlight that a firm’s cooperation with its value chain partners may weaken the unfavourable effects their high concentration. We postulate a similar effect when the speed of technological change is faster in a firm’s industry. We use secondary survey data for 770 French firms, and our findings provide broad support for our predicted relationships.

Glimpsing an exchange of partners

Chemical Physics When two diatomic molecules collide, they can sometimes swap partners. For instance, two potassium-rubidium (KRb) molecules can produce K2 and Rb2. The four-atom intermediate formed upon collision is typically too scarce and short-lived to spot, even using ultrafast techniques. Hu et al. circumvented this problem by studying the reaction at temperatures approaching 0 kelvin. Using a combination of mass spectrometry and velocity-map imaging, the authors directly characterized the ionized K2Rb2 complex as well as the reactant and product populations. Science , this issue p. [1111][1] [1]: /lookup/doi/10.1126/science.aay9531

The Export Determinants of Indonesian Cut Flower in The International Market

Trade statistics of Indonesian cut flower indicates the potential of Indonesia as an exporting country. The international market becomes more attractive as the increasing trend of demand and the rising cost for producing cut flowers shows the various challenges for emerging countries. This study investigates whether the analytical gravity model fixed effect, common effect or random effect model is proper and what determinants have significant effects to the exports of Indonesian cut flower to partner countries. The data encompasses the period of 2008 to 2017 as the series data and the seven destination export countries from Indonesia as the cross-sectional data, using the panel regression with the basic gravity model. The estimation results show the fixed effects model is the proper model to explain the determinants of bilateral export on cut flower. The estimates imply the corroborate signs with GDP per capita of Indonesia, GDP per capita of partner countries and exchange rate while those variables with the opposite sign are distance and trade openness. Thus, the export promotion, quality improvement and technology development are required in the development of export of cut flower industries.

Parental incubation exchange in a territorial bird species involves sex-specific signalling

BackgroundEffective communication between sexual partners is essential for successful reproduction. Avian parents with biparental incubation need to know how to negotiate, when and who will incubate, and how to harmonize partner exchange at the nest. Although considerable effort has been dedicated to studies of incubation rhythms, few studies have investigated how behavioural signals serve to tighten cooperation between parents. Moreover, existing studies are almost exclusively restricted to species in which long distances between incubating and non-incubating parents prevent continuous communication during incubation. Thus, the most frequently described parental exchange system is a simple model characterized by the return of the non-incubating parent to the nest itself. Here, we propose more complex parental exchange behaviour in the Northern Lapwing (Vanellus vanellus), a territorial species capable of continuous partner communication during incubation and with a highly variable male contribution to incubation.ResultsNorthern Lapwing females regularly vocalized shortly before departing from the nest, while males mostly left the nest quietly. Responsiveness of the male to female vocalization, perhaps in combination with her flying away from the nest, helped to synchronize incubation care by increasing the probability of exchange, and also by shortening the exchange gaps. In contrast, a male-to-female exchange gap most often occurred after the male quietly flew away from the nest. The frequency of female vocal signalling was not correlated with the male incubation effort on a between-nest scale, but the highest probability of a female-to-male exchange occurred after vocal signalling by females with the most nest-attentive males. Conversely, lowered effort by females to vocalize in the night was accompanied by lower willingness of males to incubate.ConclusionsOur results suggest that (1) that the incubating parent can communicate with the non-incubating partner using sex-specific behavioural signals, and this helps to synchronize parental exchange on the nest, (2) this signalling may combine acoustic and visual cues, and (3) the efficiency of this signalling might influence the overall nest attendance. The presumption that the repertoire of behavioural signals during reproduction will be much more complex in territorial species that are capable of continuous communication between the partners during the incubation period should be further tested.

Global Value Chain Participation and Exchange Rate Pass-Through

This paper draws a causal link between the rise of global value chain participation and the decline of exchange rate pass-through to import prices over the last decades. We first present a structural two-country model in order to illustrate how participation in global value chains can impact exchange rate pass-through to import prices. In the model, the sensitivity of an economy's domestic-currency production costs to exchange rate changes rises as it participates more in global value chains by importing a larger share of its intermediate inputs. The increased sensitivity of the economy's domestic-currency production costs to exchange rate changes translates into a higher sensitivity of its domestic-currency export prices. The latter implies a reduction of the sensitivity of the economy's foreign-currency export prices – i.e. its trading partner's local-currency import prices – to exchange rate changes. Hence, an increase in the economy's global value chain participation implies a fall in its trading partner's exchange rate pass-through to import prices. We then provide empirical evidence in a cross-country panel dataset for the time period from 1995 to 2014 that is consistent with the mechanisms spelled out in the structural model. In particular, the data suggest that exchange rate pass-though to export prices is higher in economies which participate more in global value chains, and that exchange rate pass-though to import prices is lower in economies whose trading partners participate more in global value chains. Quantitatively, our estimates imply that the rise in global value chain participation can account for about 50% of the decline in exchange rate pass-through to import prices since the mid-1990s.

Horizontal partner exchange does not preclude stable mutualism in fungus-growing ants

Horizontal partner exchange does not preclude stable mutualism in fungus-growing ants