Excess Organic Matter Research Articles

Organic matter ingestion and assimilation rates by the sea cucumber Holothuria (Holothuria) tubulosa (Gmelin, 1788) at different temperatures and potential effects on benthic trophic status

We investigated the effects of different temperature anomalies (up to +12 °C) on the biochemical features of sediments offered to specimens of the sea cucumber Holothuria (Holothuria) tubulosa (Gmelin, 1788) and of its feces. We also estimated ingestion and assimilation rates of different classes of organic compounds after the different treatments. The exposure to temperature anomalies influenced the metabolism and organic matter assimilation efficiency of H. tubulosa. Counterintuitively, our results would suggest that H. tubulosa, irrespectively of temperature, might not be the best option for removing excess organic matter from eutrophicated sediments, though having consequences on the organic matter nutritional quality. Since oceans’ temperature is projected to further increase by 2100 along with frequency, intensity and duration of marine heatwaves, H. tubulosa will most likely experience a potential contraction of its distribution areal, with cascading consequences on benthic trophic webs dynamics.

Analysis of Controlling Factors of Pore Structure in Different Lithofacies Types of Continental Shale—Taking the Daqingzi Area in the Southern Songliao Basin as an Example

Due to the influence of terrigenous debris, the internal pore structure of continental shale is highly heterogeneous, and the controlling factors are complex. This paper studies the structure and controlling factors of shale reservoirs in the first member of the Qingshankou Formation in the Southern Songliao Basin using core data and various analytical test data. The results show that the original deposition and subsequent diagenesis comprehensively determine the shale reservoirs’ pore structure characteristics and evolution law. According to the severity of terrigenous debris, the shale reservoirs in the study area are divided into four categories and six subcategories of lithofacies. By comparing the characteristics of different shale lithofacies reservoirs, the results show that the lithofacies with a high brittle mineral content have more substantial anti-compaction effects, more primary pores to promote retention and a relatively high proportion of mesopores/macropores. Controlling the organic matter content when forming high-quality reservoirs leads to two possibilities. An excessive organic matter content will fill pores and reduce the pore pressure resistance. A moderate organic matter content will make the inorganic diagenesis and organic hydrocarbon generation processes interact, and the development of organic matter mainly affects the development of dissolution pores. The comprehensive results show that A3 (silty laminated felsic shale) reservoirs underwent the pore evolution process of “two drops and two rises” of compaction, cementation and pore reduction, dissolution and pore increase, and organic matter cracking and pore increase, and they are the most favourable lithofacies of the shale reservoirs in the study area.

A Review Paper on Bioremediation, A Panacea to Aquaculture Productivity

Aquaculture production encompasses the cultivation of aquatic organisms, including fish, shellfish, and plants, within controlled environments, playing a pivotal role in meeting the escalating global demand for fish and seafood. Various techniques are employed, ranging from fish farming in ponds, cages, or tanks to the cultivation of seaweed and other aquatic flora. The overarching objective is to achieve sustainable production while minimizing adverse environmental impacts. The utilization of bioremediation techniques in aquaculture entails harnessing microorganisms to degrade pollutants and ameliorate water quality, thereby fostering a healthier environment conducive to the thriving of aquatic organisms. Strategic interventions, such as the introduction of specific bacteria or plants capable of absorbing excess nutrients, contribute to ecosystem equilibrium, thereby promoting sustainable aquaculture practices. Leveraging living organisms to detoxify or eliminate pollutants represents a promising strategy for addressing environmental challenges associated with intensive aquaculture. The review delves into elucidating the mechanisms underlying microbial communities and selected organisms' capacity to mitigate water quality issues, notably excess nutrients and organic matter accumulation. Common bioremediation approaches encompass the utilization of beneficial bacteria, denitrifying bacteria, Nano remediation, biological filters, bioaugmentation, and oxygenation. By integrating these bioremediation techniques, aquaculture enterprises can bolster water quality, mitigate disease risks, and foster a more sustainable and productive aquatic environment conducive to the flourishing of aquatic organisms.

Utilizing recycled pumice and oyster shell waste for cost-effective treatment to mitigate pollutants and toxicity in swine wastewater

Despite the abundant nutrients that could be reutilized in swine wastewater, inadequate wastewater management leads to excessive metals and organic matter, causing environmental impacts on aquatic and terrestrial ecosystems. In this study, we recycled waste pumice and oyster shells for the cost-effective treatment and reclamation of swine wastewater. The toxicity of the treated wastewater was assessed using the soil nematode Caenorhabditis elegans and Chinese cabbage Brassica rapa chinensis. Our findings showed significant removal of suspended solids, biochemical oxygen demand, chemical oxygen demand, total phosphorus, and heavy metals (As, Cu, Ni, and Zn) from the swine wastewater after treatment with pumice and oyster shells. Moreover, untreated wastewater significantly inhibited the germination of Chinese cabbage, a trend that was reversed in treated wastewater. Both treated and untreated swine wastewater stimulated the growth of Chinese cabbage. Additionally, untreated swine wastewater exhibited high toxicity to the growth and reproduction of C. elegans after 72 hours of exposure, whereas treated wastewater showed notably reduced toxicity. The recycled pumice and oyster shells significantly induced growth and showed no toxicity in Chinese cabbage. These results suggest that pumice and oyster shell waste can effectively reduce environmental toxicity in raw swine wastewater, offering a cost-effective wastewater treatment solution for small-scale pig farms.

Toward a Brighter Future: Enhanced Sustainable Methods for Preventing Algal Blooms and Improving Water Quality

This comprehensive review explores the escalating challenge of nutrient enrichment in aquatic ecosystems, spotlighting the dire ecological threats posed by harmful algal blooms (HABs) and excessive particulate organic matter (POM). Investigating recent advancements in water treatment technologies and management strategies, the study emphasizes the critical need for a multifaceted approach that incorporates physical, chemical, and biological methods to effectively address these issues. By conducting detailed comparative analyses across diverse aquatic environments, it highlights the complexities of mitigating HABs and underscores the importance of environment-specific strategies. The paper advocates for sustainable, innovative solutions and international cooperation to enhance global water quality and ecosystem health. It calls for ongoing advancement, regular monitoring, and comprehensive research to adapt to emerging challenges, thus ensuring the preservation of aquatic biodiversity and the protection of communities reliant on these vital resources. The necessity of integrating technological innovation, ecological understanding, and global cooperation to safeguard aquatic ecosystems for future generations is paramount.

Combined Earth observations reveal the sequence of conditions leading to a large algal bloom in Lake Geneva

Freshwater algae exhibit complex dynamics, particularly in meso-oligotrophic lakes with sudden and dramatic increases in algal biomass following long periods of low background concentration. While the fundamental prerequisites for algal blooms, namely light and nutrient availability, are well-known, their specific causation involves an intricate chain of conditions. Here we examine a recent massive Uroglena bloom in Lake Geneva (Switzerland/France). We show that a certain sequence of meteorological conditions triggered this specific algal bloom event: heavy rainfall promoting excessive organic matter and nutrients loading, followed by wind-induced coastal upwelling, and a prolonged period of warm, calm weather. The combination of satellite remote sensing, in-situ measurements, ad-hoc biogeochemical analyses, and three-dimensional modeling proved invaluable in unraveling the complex dynamics of algal blooms highlighting the substantial role of littoral-pelagic connectivities in large low-nutrient lakes. These findings underscore the advantages of state-of-the-art multidisciplinary approaches for an improved understanding of dynamic systems as a whole.

Influences of Cluster Thinning on Fatty Acids and Green Leaf Volatiles in the Production of Cabernet Sauvignon Grapes and Wines in the Northwest of China.

Cluster thinning has been widely applied in yield management and its effect on green leaf volatiles (GLVs) in wines has seldom been studied. GLVs are important flavor compositions for grapes and wines. This work aimed to investigate the impact of cluster thinning on these volatiles and their precursors in grapes and wines. Severe cluster thinning (CT1) and medium cluster thinning (CT2) were performed on Cabernet Sauvignon (Vitis vinifera L.) vines in two sites (G-farm and Y-farm) from Xinjiang province in the Northwest of China. The impact of cluster thinning treatments on the accumulation of GLVs and their precursors, long chain fatty acids (LCFAs) of grape berries and C6 volatiles, in resulting wines was investigated. Multivariate analysis showed that cluster thinning treatments induced significant changes in fruit and wine composition in both farms. In Y-farm, medium cluster thinning (CT2) significantly increased the average cluster weight of harvested berries. Additionally, both cluster thinning treatments (CT1 and CT2) increased fatty acids in harvested berries and CT2 led to an increase in C6 esters and a decrease in C6 alcohols in the wines of Y-farm under the warmer and drier 2012 vintage. However, the effect of cluster thinning was likely negative in G-farm due to its wetter soil and excessive organic matter. The treatments may be applicable for local grape growers to improve viticultural practices for the more balanced vegetative and reproductive growth of Cabernet Sauvignon grapevines. This work also provided further knowledge on the regulation of fatty acids and the derived C6 volatiles through the lipoxygenase (LOX) pathway.

Analysis of the properties of chemical content and the use of local material Kinang Jingkion as coarse aggregate against the characteristics of WC last (AC) mixture in Yalimo Regency

This study aims to analyze the nature of chemical content, analyze abrasion value and optimum asphalt content using local Kinang Jingkion as road pavement material. In this study, XRF and XRD testing methods were used to determine the chemical content of Kinang Jingkion local material, then testing the characteristics of rough aggregate to determine the abrasion value, testing fine aggregate characteristics, and testing asphalt pen 60/70 and marshall test to determine the optimum asphalt content (KAO). The tests obtained the results of comparative data analysis between laboratory tests and literature standards that meet the eligibility requirements. The results showed that: First, the results of XRF and XRD analysis on Wilihingke samples, 0.15 mm granules, 2.36 mm granules, and Kinang Holuwon found that all chemical elements contained in the four samples were solid metal inorganic chemical elements. The results of this analysis prove that the four samples, which are fine aggregates and coarse aggregates, do not contain organic substances. Where the results of this analysis are by the requirements of aggregates which are one of the components in the concrete mixture, where the aggregates used must meet certain conditions, one of which must not contain excess organic matter because it can undergo decay by bacteria using dissolved oxygen; Second, from the test results obtained, the aggregate wear value (abrasion) was 32.69%; Third, based on the test results, the value of the Marshall parameter with Optimum Asphalt Content (KAO) obtained a value of 6.125%.

Preliminary spectroscopic investigation of a potential Mars analog site: Lake Bagno dell’Acqua, Pantelleria, Italy

In this study, we characterize the mineralogy and microbiology of a suite of samples from the Bagno dell’Acqua alkaline (pH ≥ 9) lake in Pantelleria, Italy. The aim of the present characterization is the assessment of the lake as a Martian analog site. Nineteen samples were characterized by a combination of VNIR reflectance spectroscopy, micro-Raman (μ-Raman) spectroscopy, and fluorescence microscopy. In addition, DNA extraction was performed on three selected samples to identify the bacteria phyla and to quantify their abundance. The samples were collected as push cores inside the lake and as loose sediments taken along the eastern and southeastern lake's shore. The samples underwent three thermal cycles (between 343 K and 473 K) to remove excess water and organic matter. The VNIR spectra were measured on each sample after each thermal cycle to check the variation of the spectral features as a function of thermal treatment. The mineralogical content of our push core samples is dominated by the presence of Mg-smectite clays always associated with the remanence of K-feldspars and Ca-carbonates while in the sediments we observe also additional minor phases like pyroxene (aegirine NaFe3+Si2O6), hematite (Fe2O3), anatase (TiO2), and volcanic glass. Two organic phases were detected by μ-Raman spectroscopy. One phase was attributable to carotenoids, in line with the high abundance of Bacterial Phyla that can synthesize these molecules. The second phase appeared to be visually indistinguishable from the inorganic matrix and is therefore attributed to EPS (Extracellular Polymeric Substances) forming a biofilm and extensively observed by fluorescent microscopy. The peculiar chemistry of the waters, the presence of Mg-smectite in the sediments of Lake Bagno dell’Acqua associated with Ca-carbonates and sediments derived by biological activity makes Lake Bagno dell’Acqua a very interesting and promising Martian analog site.

Impact of Conditioning Agent Addition Sequence on Dewatering Performance of Advanced Anaerobic Digested Sludge

The advanced anaerobic digestion process enhances sludge resource utilization. However, thermal hydrolysis pretreatment of anaerobically digested sludge reduces dewatering efficiency due to excessive organic matter decomposition. This necessitates significant time and effort for sludge conditioning in wastewater treatment plants. Using conditioning agents can achieve high dewatering efficiency. This study investigates how the order of adding coagulants and flocculants impacts the dewatering performance of digested sludge. The results indicate that, compared to the flocculation–coagulation process with the same dosage, the coagulation–flocculation process leads to a 15–20% increase in the average particle size of digested sludge. The content of polysaccharides and proteins in S-EPS decreases by 28.8–30.8% and 10.1–11.3%, respectively. The filter cake solids content increases by 8.5%, and there is an increase in surface water channels within the flocs. This is because initially adding coagulants efficiently adsorbs small particles, forming larger aggregates that settle effectively. This promotes the breakdown of extracellular polymeric substances, releasing more bound water. Adding flocculants later bridges the aggregates, further enhancing settling and filtration performance, thereby improving sludge dewatering efficiency. These research findings contribute to a better understanding of the mechanisms of coagulant and flocculant co-conditioning for digested sludge and provide recommendations for optimizing sludge conditioning steps.

Anaerobic digestion of vinasse improves its use as a liquid fertilizer

The global demand for exploiting the potential of renewable biofuel production is increasing. When countries with large agricultural areas produce ethanol, they also generate a harmful byproduct called vinasse. Vinasse is a dark brown effluent that contains a high amount of organic matter, has an acidic nature, and is rich in mineral salts. Managing this effluent poses a challenge due to its significant volume, which ranges from 8 to 20 liters per liter of ethanol produced. Traditionally, raw vinasse has been applied directly to the soil as a liquid fertilizer. However, this practice, without proper guidance, can lead to soil degradation, micronutrient imbalance, pH change and excessive accumulation of organic matter. To resolve these issues and still use vinasse as a fertilizer, the process of anaerobic degradation of vinasse in biodigesters was analyzed. The resulting treated effluent, known as digested vinasse, was then compared to raw vinasse in terms of pollution parameters and the balance of minerals crucial for sugarcane cultivation. The results demonstrated a significant reduction in the organic load of vinasse, with a removal of chemical oxygen demand (COD) ranging from 66% to 85%. After applying the digested vinasse to sugarcane like cultivation soil, a noticeable decrease in the environmental impact caused by excessive organic matter was observed. Additionally, the study found that important nutritional elements such as sodium (Na) did not have any adverse effects or accumulate excessively when compared to calcium (Ca) and magnesium (Mg). In conclusion, the research demonstrates that the anaerobic degradation of vinasse in biodigesters can effectively reduce its organic load and mitigate the environmental impact associated with the direct application of raw vinasse to agricultural soils

Threats to the Lower Section of the River after Fish Mortality in the Ecological Environment of the Oder River

This work presents a case study accompanied by an analysis of potential reasons for fish mortality in the lower Oder River during an ecological disaster in August 2022. Changes in physicochemical water quality indicators indicate that fish and benthic organism death was likely due to the creation of hypoxia. The research also highlighted the trophic threats to the studied Oder River section linked to the disaster. The disaster occurred in the upper and middle Oder River, extending to the lower stretch. A physicochemical analysis of water samples revealed that the fish and organism death in the estuarine section was mainly due to reduced dissolved oxygen, caused by the decomposition of organic matter from the upper river sections. This was reinforced by high temperatures and low water levels. The negative trophic consequences of the described ecological disaster for the lower section of the Oder River may be long-term. Over thirty years of monitoring showed threats of excess biogenic compounds, organic matter, and bacterial contamination. Despite decreasing nitrogen and phosphorus, significant phytoplankton blooms implied internal nutrient sources. Considering climate change and the poor trophic status, recurrent disasters and deteriorating conditions are anticipated. Mitigation requires continuous monitoring, limiting the nutrient input (especially phosphorus), and preparing for extreme events. Geotechnical methods in the lower river can improve oxygen levels and fish survival.

Determining the optimal application rate of chicken manure for agricultural land through Phosphorus sorption-desorption analyses in Andisols of Wonokitri, East Java, Indonesia

Phosphorus (P) is a macro-essential element extensively used in agricultural production. Andisols, commonly found in Indonesia, serve as agricultural areas with high, excessive, and continuous organic matter input. However, the propensity of the soil for P leaching poses a potential risk of water pollution and eutrophication in the watershed area. This study aimed to evaluate the impact of the application rate of organic matter, specifically chicken manure, often employed in agricultural activities related to Andisols. The experiment involved observing P sorption, P desorption, P sorption kinetics, and P uptake by wheat (<em>Triticum aestivum</em>) in soil incubated with chicken manure added at various rates of 0, 10, 20, 30, and 40 t ha<sup>-1</sup>. The incubation stages were conducted for 14 days at room temperature (27°C) and soil moisture was maintained within field capacity. The data collected were analyzed using Langmuir isotherm for P sorption and desorption, and first-order kinetics for P sorption kinetics. The results showed that the chicken manure addition at 10-40 t ha<sup>-1</sup> significantly reduced P bonding energy in Andisols, but failed to decrease the sorption maxima value due to accumulated P from previous applications performed. The application of 20 t ha<sup>-1</sup> (CM20) of chicken manure was found to be the optimal rate, displaying high P uptake and reduced bonding energy, while rates above 20 t ha<sup>-1</sup> showed no significant difference in P uptake and bonding energy levels. Therefore, CM20 was recommended to increase P availability and prevent P movement into water bodies, promoting sustainable agricultural practices.

Elevated temperatures increase abnormalities in embryos and reduce larval survival in serpulid polychaetes.

Environmental temperature is one of the most significant factors influencing the developmental rate and survival of embryos and larvae of many marine animals, including polychaetes. The aim of this study was to experimentally evaluate the effect of temperature increase on the embryonic development and larval survival of Spirobranchus incrassatus and S. cf. corniculatus. Adult worms of both species were collected from the western margin of the Gulf of Tehuantepec, Mexico. Embryos and larvae were obtained from these worms and exposed to four temperature treatments (28, 30, 32 and 34°C). The optimal temperatures for embryonic development of S. incrassatus and S. cf. corniculatus were 30 and 28°C, respectively. For both species, the maximum critical temperature was 32°C and the lethal temperature was 34°C. The embryonic stages of S. cf. corniculatus were most negatively affected by elevated temperatures. Larval survivorship of S. incrassatus and S. cf. corniculatus was higher at 30°C and 28°C (76.2±2.8%; 72.6±4.2%) and lower at 34°C (28.9±2.6%; 26.3±2.9%), respectively. These results suggest that S. cf. corniculatus has the lowest thermal tolerance. Both Spirobranchus species already live near their upper limit of thermal tolerance in the study region (30°C). In the near future, under a global warming scenario, the distribution of both species could be modified, causing a loss of biodiversity, changes in the trophic chain, and alterations in the water column, such as excess organic matter.

Effect of Tubifex tubifex on the purification function of saturated vertical flow constructed wetlands for effluents with varying C/N ratios

The improvement effect of Tubifex tubifex on the pollutant removal efficiencies (REs) of vertical flow constructed wetlands (VF-CWs) treating wastewater with various C/N ratios was explored. The experiment was conducted in pilot-scale saturated VF-CWs, being added different densities of T. tubifex and fed synthetic wastewater with successive C/N ratios of 0.5, 1.5, 3.0 and 6.0. The results suggest that T. tubifex addition and the influent C/N ratio had an interactive effect, i.e., T. tubifex addition improved NOx−-N, NH4+-N, TN and COD REs by 36.7%, 56.5%, 22.6%, and 10.0%, respectively, under low C/N ratios, while high C/N ratios inhibited this improvement. Low-density T. tubifex addition significantly increased substrate dissolved oxygen (DO) by retarding excessive soil organic matter (OM) accumulation. With T. tubifex addition, an improvement in bacterial diversity, the relative abundance of N-cycle and fermentative bacteria, and N-cycle functional genes was only observed in substrates under low C/N ratios. T. tubifex can improve the purification function of saturated VF-CWs, but this strategy strongly depends on both the influent C/N ratio and density of T. tubifex addition.

Acidogenesis of Pentose Liquor to Produce Biohydrogen and Organic Acids Integrated with 1G–2G Ethanol Production in Sugarcane Biorefineries

Second-generation (2G) ethanol production has been increasingly evaluated, and the use of sugarcane bagasse as feedstock has enabled the integration of this process with first-generation (1G) ethanol production from sugarcane. The pretreatment of bagasse generates pentose liquor as a by-product, which can be anaerobically processed to recover energy and value-added chemicals. The potential to produce biohydrogen and organic acids from pentose liquor was assessed using a mesophilic (25 °C) upflow anaerobic packed-bed bioreactor in this study. An average organic loading rate of 11.1 g COD·L−1·d−1 was applied in the reactor, resulting in a low biohydrogen production rate of 120 mL·L−1 d−1. Meanwhile, high lactate (38.6 g·d−1), acetate (31.4 g·d−1), propionate (50.1 g·d−1), and butyrate (50.3 g·d−1) production rates were concomitantly obtained. Preliminary analyses indicated that the full-scale application of this anaerobic acidogenic technology for hydrogen production in a medium-sized 2G ethanol distillery would have the potential to completely fuel 56 hydrogen-powered vehicles per day. An increase of 24.3% was estimated over the economic potential by means of chemical production, whereas an 8.1% increase was calculated if organic acids were converted into methane for cogeneration (806.73 MWh). In addition, 62.7 and 74.7% of excess organic matter from the 2G ethanol waste stream could be removed with the extraction of organic acid as chemical commodities or their utilization as a substrate for biomethane generation, respectively.

Modern hydrological characteristics of the Kuibyshev reservoir as a basis for the development of water bioresources

THE PURPOSE. Consider the indicators of the operation mode of the Kuibyshev reservoir over the past 15 years, affecting the state of the fish stock; identify the main trends in changes in water volumes in the reservoir due to climate change, the features of the level regime for the ecological solution of the problem of preserving aquatic biological resources.METHODS. A comparative analysis of our own research and literature data on the hydrological characteristics of the Kuibyshev reservoir, the temperature regime of the region and the state of the ichthyofauna was carried out. In this work, to analyze the current state of the hydrological regime, data provided by the Verkhniy Byef gauging station of the branch of JSC RusHydro - Zhigulevskaya HPP and the State Committee of the Republic of Tatarstan for Biological Resources were used.RESULTS. It is shown that the average water level in the Kuibyshev reservoir for the last period ranged from 50.34 to 52.31 m at NHL - 53.00 m BS, DVL - 45.5 m. Based on the totality of characteristics in terms of average water levels and inflows, 2010, 2011, 2021 can be attributed to low-water years, 2007-2009, 2012-2020 to years with average water content, and there were no high-water years during this period.The analysis of the dynamics of changes in the maximum and minimum water levels was carried out and changes were identified: the minimum recorded water level (48.16 m) was noted in January (2011), at the end of the 20th century, the minimum levels were recorded in March - April and they were lower by 1-1, 5 m in summer drawdown and 2.5 - 3.5 m in winter, the level rise occurs in April, while earlier it was in May. The duration of the high-water period (about 53 m) increased to 4 months. The minimum volume in the reservoir was recorded in the winter period of 2010-2011 - 36.14 km3, the maximum volume is 57.66 km3 in the winter period of 2019-2020. Maximum water discharges are carried out mainly in the second half of April and early May. Minimum discharges are carried out in different periods.It is shown that the number of days with an air temperature above 15°C, which characterizes the fishery zone, varies in different years of the last period from 71 days (2017) to 133 days (2020).CONCLUSION. A relative increase in temperature was noted as the most important environmental factor for the development of aquatic bioresources; currently, the process of accelerating eutrophication of shallow areas of the Kuibyshev reservoir is observed. The regular death of fish in different parts of the reservoir, combined with factors such as low water levels and high temperatures, creates the preconditions for “local catastrophes” at the level of ichthyocenoses, the area of “local catastrophes” is expanding due to climate change. The mortality of fish is a mechanism for the release of the ecosystem from excess organic matter, and the mortality of a small particle indirectly indicates a high abundance of low-value and weedy fish, the number of which must be reduced.

Biodeposit dispersion around a deep cage finfish farm in the Northern Persian Gulf

Due to increasing demand for food, intensive mariculture of finfish is a fast developing industry in the Iranian part of the Persian Gulf (PG). The environmental impacts of cage fish farming largely involve release of excessive organic matter (biodeposits). Careful monitoring of fish farms to avoid anoxia and bottom “dead zones” is an essential part of a sustainable mariculture industry.Monitoring data were collected for a fish farm north of Kish Island that had been in full operation for a period of one year. The data were then incorporated into a hydrodynamic model in order to estimate the quantity and dispersion pattern of biodeposits around the farm.Our results show that the farm had significant impacts on local environmental conditions, including water transparency, sulfide levels, and Eh values. Bottom water clarity near the cages was >15 NTU. Underneath the cages, sediment sulfide values are >800 μmole, Eh is usually -100 or less, and the sedimentation rate is 6.8 g/m2/day. The number of live benthic foraminifera near the cages was 4.5/10 g aliquot, as opposed to 20 at a control site.Numerical modeling, combined with data from sediment traps, shows that the farm “footprint” is much larger than the actual area of the farm, and is elongated by tidal currents. Considering the relatively short operation of the farm, it is already close to generating anoxic bottom conditions over a 2 km wide area. This can potentially impact the fragile habitats of the Persian Gulf. Continued expansion of net pen fish farms in sensitive areas such as the PG should be carefully monitored.

Exploiting Polyhydroxyalkanoates for Biomedical Applications.

Polyhydroxyalkanoates (PHA) are biodegradable plastic. Numerous bacteria produce PHAs under environmental stress conditions, such as excess carbon-rich organic matter and limitations of other nutritional elements such as potassium, magnesium, oxygen, phosphorus, and nitrogen. In addition to having physicochemical properties similar to fossil-fuel-based plastics, PHAs have unique features that make them ideal for medical devices, such as easy sterilization without damaging the material itself and easy dissolution following use. PHAs can replace traditional plastic materials used in the biomedical sector. PHAs can be used in a variety of biomedical applications, including medical devices, implants, drug delivery devices, wound dressings, artificial ligaments and tendons, and bone grafts. Unlike plastics, PHAs are not manufactured from petroleum products or fossil fuels and are, therefore, environment-friendly. In this review, a recent overview of applications of PHAs with special emphasis on biomedical sectors, including drug delivery, wound healing, tissue engineering, and biocontrols, are discussed.

Integrated multitrophic culture of shrimp Litopenaeus vannamei and tilapia Oreochromis niloticus in biofloc system: A pilot scale study

Shrimp production in biofloc systems generates excess organic matter that must be removed from the system. Due to its ability to consume natural productivity, the integration of tilapia in shrimp culture could help to reduce the levels of total suspended solids in the biofloc system. The present study aimed to evaluate two stocking densities of tilapia in an integrated culture with shrimp Litopenaeus vannamei reared in a pilot-scale biofloc system. Two stocking densities of tilapia were tested, 35 and 65 fish m-3 in a recirculating system with 10 m3 tanks for shrimp culture and 4 m3 for tilapia culture with water recirculation of 965.66 ± 92.83 L h-1 during 78 days. The initial weight of shrimp was 0.9±0.1 g and of tilapia was 7.1±3.2 g. Shrimps were fed according to the feeding table and fish were underfed to stimulate bioflocs consumption. Selected water quality parameters were monitored during the trial. Tilapia densities did not affect shrimp growth (11.5±1.9 g and 10.1±0.7 g for 35 and 65 fish m-3 treatments, respectively). The tilapia presented a FCR lower than 1, proving that bioflocs were consumed by fish. The clarification time was shorter when compared to other studies with shrimp monoculture. Between the treatments, a reduction of 10 hours in the system clarification occurred when lower fish stocking density was used. The results demonstrate the feasibility of integrated shrimp and tilapia culture on a pilot scale, without compromising shrimp productivity.