Direct Evidence Research Articles

Halobenzoquinone-induced potential carcinogenicity associated with p53-mediated cell cycle pathway

2,6-Dibromo-1,4-benzoquinone (2,6-DBBQ) and 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ), two emerging halobenzoquinones (HBQs), have the highest detection frequencies and levels in drinking water among all HBQs. They are more toxic than the regulated disinfection byproducts. Quantitative structure toxicity relationship analysis predicted that HBQs are a class of potential bladder carcinogens. However, direct experimental evidence for the carcinogenicity of 2,6-DBBQ and 2,6-DCBQ is lacking and the associated toxicity mechanisms remain unclear. In this study, we confirmed the potential carcinogenicity of 2,6-DBBQ and 2,6-DCBQ using an in vitro malignant transformation assay, evaluated their cytotoxicity and genotoxicity, and investigated their toxicity mechanisms. The results showed that 2,6-DBBQ and 2,6-DCBQ significantly decreased the viability of human uroepithelial SV-HUC-1 cells and induced DNA damage in SV-HUC-1 cells, and chromosomal damage in HepG2 cells, and malignant transformation of SV-HUC-1 cells. Moreover, transcriptome sequencing revealed that 2,6-DBBQ and 2,6-DCBQ activated the p53-mediated cell cycle pathway in bladder cancer. In the p53-mediated cell cycle pathway, 2,6-DBBQ and 2,6-DCBQ induced cell cycle arrest at the S phase by downregulating p53 and upregulating p21. Additionally, 2,6-DBBQ and 2,6-DCBQ may have produced excessive reactive oxygen species, damaging DNA and chromosomes. These results not only first confirm the potential carcinogenicity of 2,6-DBBQ and 2,6-DCBQ but also provide an important reference for exploring the cytotoxicity and genotoxicity mechanisms of these HBQs.

Fishing or farming? Isotopic evidence of human subsistence strategies at the Dashuitian site during the middle Neolithic in the Three Gorges of the Yangtze River, China

The Three Gorges of the Yangtze River, with its deep canyons, abundant freshwater resources, and unique geographic location at the crossroads in the spread of rice-millet agriculture in the Middle Neolithic, is of great significance for understanding the interaction between fishing-hunting-gathering and farming in inland freshwater environments. However, few direct evidence for human subsistence strategies had been published in this region. This study presents results of carbon and nitrogen stable isotope analyses on human bones, animal and millet remains from the Dashuitian site (c. 6000–5500 BP) in the Three Gorges of the Yangtze River, China, to investigate the diet and subsistence and intrapopulation dietary variations in this region. The results indicate that the humans at the site consumed mostly freshwater fish foods and supplemented by terrestrial animals, with no discernable input from millets. They had consistently relied on a fishing, hunting, and gathering economy during the occupation of the site in the Middle Neolithic (c. 6000–5500 BP), differing from humans in other areas especially along the rivers and coasts of the lower Yangtze River valley for the roughly same period, further showing the extensive utilization of and adaptation to the available environmental resources. In addition, intrapopulation dietary variations based on burial style and sex provide evidence that differentiation and inequality, at least regarding to food consumption, existed at the Three Gorges region during the Middle Neolithic. Here, our findings provide isotopic evidence about the Middle Neolithic human subsistence strategies in the Three Gorges of the Yangtze River, providing a glimpse into the complexity related to inequality in food access among fisher-hunter-gatherers in inland areas, and new insights into understanding past human-environment interactions.

Using the Ca ii Lines in T Tauri Stars to Infer the Abundance of Refractory Elements in the Innermost Disk Region

We present a study of the abundance of calcium in the innermost disk of 70 T Tauri stars in the star-forming regions of Chamaeleon I, Lupus, and Orion OB1b. We use calcium as a proxy for the refractory material that reaches the inner disk. We used magnetospheric accretion models to analyze the Ca ii emission lines and estimate abundances in the accretion flows of the stars, which feed from the inner disks. We find Ca depletion in disks of all three star-forming regions, with 57% of the sample having [Ca/H] < –0.30 relative to the solar abundance. All disks with cavities and/or substructures show depletion, consistent with trapping of refractories in pressure bumps. Significant Ca depletion ([Ca/H] < –0.30) is also measured in 60% of full disks, although some of those disks may have hidden substructures or cavities. We find no correlation between Ca abundance and stellar or disk parameters except for the mass accretion rate onto the star. This could suggest that the inner and outer disks are decoupled, and that the mass accretion rate is related to a mass reservoir in the inner disk, while refractory depletion reflects phenomena in the outer disk related to the presence of structure and forming planets. Our results of refractory depletion and timescales for depletion are qualitatively consistent with expectations of dust growth and radial drift, including partitioning of elements, and constitute direct evidence that radial drift of solids locked in pebbles takes place in disks.

Limited Evidence Base for Determining Impacts (Or Not) of Offshore Wind Energy Developments on Commercial Fisheries Species

ABSTRACTThe coexistence between offshore wind and fisheries has raised questions about potential impacts on species that are fished. We systematically evaluated the offshore wind farm (OWF) literature for evidence of effects leading to impacts on commercial fisheries species. First, we collated evidence of environmental effects of OWFs on fisheries species and then determined whether these could be interpreted as impacts using fishery‐scale and organism‐scale parameters for pelagic finfish, demersal and reef‐associated roundfish, demersal flatfish, elasmobranchs and shellfish. We appraised consistency and level of agreement of direct evidence and explored the body of indirect evidence. A total of 1268 documents featured evidence of OWF effects on fisheries species, with only 60 documents (274 species records) providing direct evidence. Evidence on finfish far outweighed that for shellfish. Demersal and reef‐associated roundfish were the best‐studied group, while elasmobranchs were poorly evidenced. Most studies considered population rather than stock parameters. There was limited evidence of impacts, owing to inconclusive results and inconsistent effects within the parameters assessed—illustrating the importance of looking across the evidence base rather than focussing on individual studies. Hence, there is currently insufficient direct evidence to confidently determine OWF impacts on fisheries species. Overwhelmingly, the evidence deals with indirect effects, although these should not be disregarded as they can highlight plausible impacts on fisheries species, which could guide research and monitoring targeted at understanding the impacts of OWF—a pressing concern given the increased policy commitment of many nations to these two marine sectors sharing marine space.

Increasing Contribution of Chlorine Chemistry to Wintertime Ozone Formation Promoted by Enhanced Nitrogen Chemistry.

Chlorine (Cl) radicals strongly affect atmospheric oxidation and the fate of pollutants. Despite several observations, the potential impacts of nitrogen chemistry associated with NO2 on Cl chemistry are poorly understood. Here, we provided direct field evidence that the nitrogen chemistry enhancements triggered by the increased NO2 drove daytime nitrate (NO3-) photolysis and nighttime NO3-N2O5 reactions, significantly promoting the increases in the concentrations of ClNO2 and Cl2 after the Chinese Spring Festival. The enhancement in the Cl chemistry facilitated the elevations of both O3 and atmospheric oxidation capacity during the winter daytime. Our findings highlighted the importance of nitrogen chemistry induced by the increased NO2 in enhanced Cl chemistry.

Effect of a SARS-CoV-2 Protein Fragment on the Amyloidogenic Propensity of Human Islet Amyloid Polypeptide.

Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the onset of COVID-19 have been linked to an increased risk of developing type 2 diabetes. While a variety of mechanisms may ultimately be responsible for the onset of type 2 diabetes under these circumstances, one mechanism that has been postulated involves the increased aggregation of human islet amyloid polypeptide (hIAPP) through direct interaction with SARS-CoV-2 viral proteins. Previous computational studies investigating this possibility revealed that a nine-residue peptide fragment known as SK9 (SFYVYSRVK) from the SARS-CoV-2 envelope protein can stabilize the native conformation of hIAPP1-37 by interacting with the N-terminal region of amylin. One of the areas particularly stabilized through this interaction encompasses residues 15-28 of amylin. Given these findings, we investigated whether SK9 could interact with short amyloidogenic sequences derived from this region of amylin. Here, we employ docking studies, molecular dynamics simulations, and biophysical techniques to provide theoretical as well as direct experimental evidence that SK9 can interact with hIAPP12-18 and hIAPP20-29 peptides. Furthermore, we demonstrate that SK9 not only can interact with these sequences but also serves to prevent the self-assembly of these amyloidogenic peptides. In striking contrast, we also show that SK9 has little effect on the amyloidogenic propensity of full-length amylin. These findings are contrary to previous published simulations involving SK9 and hIAPP1-37. Such observations may assist in clarifying potential mechanisms of the SARS-CoV-2 interaction with hIAPP and its relevance to the onset of type 2 diabetes in the setting of COVID-19.

Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells

Non-homologous end joining (NHEJ) is the predominant pathway that repairs DNA double-stranded breaks (DSBs) in vertebrates. However, due to challenges in detecting DSBs in living cells, the repair capacity of the NHEJ pathway is unknown. The DNA termini of many DSBs must be processed to allow ligation while minimizing genetic changes that result from break repair. Emerging models propose that DNA termini are first synapsed ~115 Å apart in one of several long-range synaptic complexes before transitioning into a short-range synaptic complex that juxtaposes DNA ends to facilitate ligation. The transition from long-range to short-range synaptic complexes involves both conformational and compositional changes of the NHEJ factors bound to the DNA break. Importantly, it is unclear how NHEJ proceeds in vivo because of the challenges involved in analyzing recruitment of NHEJ factors to DSBs over time in living cells. Here, we develop an approach to study the temporal and compositional dynamics of NHEJ complexes using live cell single-molecule imaging. Our results provide direct evidence for stepwise maturation of the NHEJ complex, pinpoint key regulatory steps in NHEJ progression, and allowed us to estimate the overall repair capacity of the NHEJ pathway in living cells.

Zircon trace element evidence for early hydrothermal activity on Mars.

Finding direct evidence for hydrous fluids on early Mars is of interest for understanding the origin of water on rocky planets, surface processes, and conditions essential for habitability, but it is challenging to obtain from martian meteorites. Micro- to nanoscale microscopy of a unique impact-shocked zircon from the regolith breccia meteorite NWA7034 reveals textural and chemical indicators of hydrothermal conditions on Mars during crystallization 4.45 billion years ago. Element distribution maps show sharp alternating zoning defined by marked enrichments of non-formula elements, such as Fe, Al, and Na, and ubiquitous nanoscale magnetite inclusions. The zoning and inclusions are similar to those reported in terrestrial zircon crystallizing in the presence of aqueous fluid and are here interpreted as primary features recording zircon growth from exsolved hydrous fluids at ~4.45 billion years. The unique record of crustal processes preserved in this grain survived early impact bombardment and provides previously unidentified petrological evidence for a wet pre-Noachian martian crust.

Prenatal air pollutant exposure and preterm birth risk in primiparas of advanced maternal age residing in rural Henan province

ABSTRACT Direct evidence on the impact of air pollution on preterm birth (PTB) among advanced maternal age (AMA) primiparas is limited. This study examined the association between air pollution and PTB among 6,295 AMA primiparas in rural Henan Province. Logistic regression and distributed lag nonlinear models showed that prenatal exposure to carbon monoxide (CO), sulfur dioxide (SO2), nitrogen dioxide (NO2), and fine particulate matter (PM2.5) was associated with an increased PTB risk among AMA primiparas, while ozone (O3) exposure was inversely related to PTB risk (all P < 0.05). Moreover, the vulnerable periods were identified as gestational weeks 1–6 and 22–28. The education level, smoking status, and season of conception significantly modified the association between PTB and air pollution (P interaction < 0.10) among AMA primiparas. These findings emphasize the impact of air pollution on PTB risk in AMA primiparas, highlighting the need for targeted interventions in vulnerable populations.

In Vitro Cell Model Investigation of Alpha-Synuclein Aggregate Morphology Using Spectroscopic Imaging.

Recently, it has been hypothesized that alpha-synuclein protein strain morphology may be associated with clinical subtypes of alpha-synucleinopathies, like Parkinson's disease and multiple system atrophy. However, direct evidence is lacking due to the caveat of conformation-specific characterization of protein strain morphology. Here we present a new cell model based in vitro method to explore various alpha-synuclein (αsyn) aggregate morphotypes. We performed a spectroscopic investigation of the HEK293 cell model, transfected with human wildtype-αsyn and A53T-αsyn variants, using the amyloid fibril-specific heptameric luminescent oligomeric thiophene h-FTAA. The spectral profile of h-FTAA binding to aggregates displayed a blue-shifted spectrum with a fluorescence decay time longer than in PBS, suggesting a hydrophobic binding site. In vitro spectroscopic binding characterization of h-FTAA with αsyn pre-formed fibrils suggested a binding dissociation constant Kd < 100 nM. The cells expressing the A53T-αsyn and human wildtype-αsyn were exposed to recombinant pre-formed fibrils of human αsyn. The ensuing intracellular aggregates were stained with h-FTAA followed by an evaluation of the spectral features and fluorescence lifetime of intracellular αsyn/h-FTAA, in order to characterize aggregate morphotypes. This study exemplifies the use of cell culture together with conformation-specific ligands to characterize strain morphology by investigating the spectral profiles and fluorescence lifetime of h-FTAA, based upon its binding to a certain αsyn aggregate. This study paves the way for toxicity studies of different αsyn strains in vitro and in vivo. Accurate differentiation of specific alpha-synucleinopathies is still limited to advanced disease stages. However, early subtype-specific diagnosis is of the utmost importance for prognosis and treatment response. The potential association of αsyn aggregates morphotypes detected in biopsies or fluids to disease phenotypes would allow for subtype-specific diagnosis in subclinical disease stage and potentially reveal new subtype-specific treatment targets. Notably, the method may be applied to the entire spectrum of neurodegenerative diseases by using a combination of conformation-specific ligands in a physicochemical environment together with other types of polymorphic amyloid variants and assess the conformation-specific features of various protein pathologies.

Ancient genomes from the Tang Dynasty capital reveal the genetic legacy of trans-Eurasian communication at the eastern end of Silk Road

BackgroundAncient Chang’an in the Tang Dynasty (618–907 AD) was one of the world’s largest and most populated cities and acted as the eastern end of the world-famous Silk Road. However, little is known about the genetics of Chang’an people and whether the Western Regions-related gene flows have been prevalent in this cosmopolitan city.ResultsHere, we present seven genomes from Xingfulindai (XFLD) sites dating to the Tang Dynasty in Chang’an. We observed that four of seven XFLD individuals (XFLD_1) were genetically homogenous with the Late Neolithic Wadian, Pingliangtai, and Haojiatai populations from the middle reaches of the Yellow River Basin (YR_LN), with no genetic influence from the Western Eurasian or other non-Yellow River-related lineages. The remaining three XFLD individuals were a mixture of YR_LN-related ancestry and ~ 3–15% Western Eurasian-related ancestry. Mixtures of XFLD_1 and Western Eurasian-related ancestry drove the main gradient of genetic variation in northern and central Shaanxi Province today.ConclusionsOur study underlined the widespread distribution of the YR_LN-related ancestry alongside the Silk Road within the territory of China during the historical era and provided direct evidence of trans-Eurasian communication in Chang’an from a genetic perspective.

Interlocal learning mechanisms and policy diffusion: The case of new energy vehicles finance in Chinese cities

AbstractPolicy diffusion based on learning mechanisms has fascinated political science and public administration scholars for a long time. A robust and growing body of studies have identified the existence and importance of learning mechanisms in policy diffusion. However, there are still some gaps that need to be further improved. First, scholars identify the learning mechanism mainly based on indirect evidence, such as geographical proximity and successful innovation policies adopted by other jurisdictions, which lacking direct and systematic evidence. Second, little is known about how the hierarchical power structure affects the leap from learning behavior to policy adoption. This study provides direct evidence for the promoting effect of intergovernmental learning on policy diffusion by analyzing case of Chinese local financial subsidy policies for new energy vehicles. The empirical results reveal that policy learning in the form of site visits among local governments significantly promotes the policy diffusion, but superior government policy strategy attenuates the influence of interlocal learning on policy diffusion. Also, the initiators and themes of policy learning affect the learning–diffusion linkage, portraying the conditional effects and nuanced dynamics of interlocal policy learning in eliciting policy diffusion.

Evidence for dual roles of histone H3 lysine 4 in antagonizing Polycomb group function and promoting target gene expression.

Tight control over cell identity gene expression is necessary for proper adult form and function. The opposing activities of Polycomb and trithorax complexes determine the on/off state of cell identity genes such as the Hox factors. Polycomb group complexes repress target genes, whereas trithorax group complexes are required for their expression. Although trithorax and its orthologs function as methyltransferases specific to histone H3 lysine 4 (H3K4), there is no direct evidence that H3K4 regulates Polycomb group target genes in vivo. Using histone gene replacement in Drosophila, we provide evidence of two key roles for replication-dependent histone H3.2K4 in Polycomb target gene control. First, we found that H3.2K4 mutants mimic H3.2K4me3 in antagonizing methyltransferase activity of the PRC2 Polycomb group complex. Second, we found that H3.2K4 is also required for proper activation of Polycomb targets. We conclude that H3.2K4 directly regulates Polycomb target gene expression.

Overexpression of MYB transcription factor LrAN2 from Lycium ruthenicum activated the anthocyanin biosynthesis in the leaf and fruit of Lycium barbarum

AN2 was thought to be the key gene inducing the fruit color differentiation of Lycium barbarum and Lycium ruthenicum, but there was no direct evidence. In this study, AN2 from L. ruhtenicum was overexpressed in L. barbarum to conform its function in regulating anthocyanin biosynthesis. The results showed that the stems, leaves, and fruits of transgenic plants were purple and contained significantly higher anthocyanin levels compared to the wild-type plants. Transcriptome analysis of leaf and fruit identified 5,832 differentially expressed genes (DEGs) in leaves and 2,029 DEGs in fruits, with 910 genes common to both tissues. GO and KEGG pathway enrichment analyses indicated a significant impact of LrAN2 on flavonoid biosynthesis and anthocyanin biosynthesis. The structural genes involved in the anthocyanin pathway, such as 4CL, CHS, F3H, F3’5’H, DFR, ANS, and UFGT, were up-regulated in transgenic lines, while the expression of transcription factors bHLH and WD40 were remained unchanged. These findings confirm the role of LrAN2 in the color differentiation of L. barbarum and L. ruthenicum and highlight its potential for the genetic enhancement of anthocyanin content in L. barbarum and other Solanaceae species.

Direct Covalent Functionalization of H-Terminated 2D Germanane with Thiolated Molecules: Passivation and Tuning of Optoelectronic Properties.

Covalent molecular functionalization allows the physicochemical properties of 2D materials to be precisely tuned and modulated on-demand. Nonetheless, research on the molecular functionalization of 2D monoelemental graphene-like materials─known as Xenes─remains scarce, being mainly restricted to a specific type of solid-state chemical reaction based on the topotactic transformation of bulkier Zintl phases. Herein, a robust and general chemical approach is reported for the direct functionalization of commercially available H-terminated 2D germanene (2D-GeH) with thiolated molecules (R-SH) via Ge-S bond formation. While the material characterization data provide direct experimental evidence of the Ge-S chemical bonding, density functional theory (DFT) calculations also predict its existence. Remarkably, the anchored thiolated molecules also favor the passivation of the 2D Xene against air oxidation, enlarging its benefits for real implementation. As a proof-of-principle, a redox-responsive molecular moiety such as 6-(ferrocenyl)hexanethiol (Fc6-SH) has been exploited to induce changes in the optoelectronic properties of the resulting 2D-GeFc6 heterostructure by simply modulating the external bias potential, making it possible to optically and electrically read out a molecular switch on 2D Xene via implanting molecular responsiveness. Remarkably, the ON/OFF ratio has been shown to be dependent on the distance between the redox-responsive Fc moiety and the 2D Xene surface through the alkyl chain length. Overall, the reported a-la-carte molecular engineering approach provides the basis toward the rapid development of stable 2D-GeR derivatives exhibiting molecule-programmable properties.

Microfluidic visualization of in‐situ emulsification during surfactant flooding

AbstractSurfactant plays a crucial role in the chemical enhanced oil recovery (cEOR) process, and in‐situ emulsification is regarded as one of the mechanisms for surfactant flooding. However, no direct evidences are available so far to show how emulsions are generated in porous media, and how emulsification is essential for cEOR. To address these issues, binary mixtures of several currently‐used sulfonate surfactants, SH5, SH6, and SHZ, were formulated with connate brine to displace crude oil in both 2D microfluidic chips and 3D glass beads porous media, as well as artificial cores. It was found that both “oil‐in‐water” (O/W) and “water‐in‐oil” (W/O) macroemulsions can be formed in‐situ inside the porous media, and they improve oil recovery mainly through breaking residual oil into small drops and improving mobility ratio, which can significantly reduce the residual oil saturation by up to 12.8% in 2D microchips. The in‐situ emulsification in 3D glass beads medium can get oil recovery factor up to 15% over water flooding. In‐situ formulation of microemulsion was also observed in microfluidic flow tests. Part of the oil phase is emulsified into microemulsions that are present in the middle phase, further mobilizing the oil trapped downstream, lowering residual oil saturation from 54.8% to 11.3% in 2D microchips, and enhancing oil recovery factor up to 25% in core flooding test. These findings advance insightful understanding of in‐situ emulsification during surfactant flooding process under simulated oil reservoir condition, offering real‐time evidence of how both macro‐ and micro‐emulsions formation help for cEOR.

Коротка вічність або Довгий кінець радянського академізму

This article is the Opus postum of the author, who passed away in September 2023. Volodymyr Verloka is the author of numerous translations of philosophical classics from English, worked in various academic institutions, including the Institute of Philosophy of the National Academy of Sciences of Ukraine and the V. I. Vernadskyi National Library of Ukraine. He received his philosophical education at the beginning of the 1990s and personally witnessed the Soviet period of Ukrainian philosophy. The author’s critical reflections offered here are largely the result of his own experience of participating in the work of various academic institutions of independent Ukraine, and are also an honest expression of his sincere desire to change the situation with Ukrainian science and higher education for the better. In the article, the author addresses the reconstruction of the specific understanding of science in the USSR, revealing such features as ideological, partisan, and bureaucratic. The author analyzes the documents of the era – the publication of texts by Friedrich Engels, Vladimir Lenin, Volodymyr Vernadskyi, Nikolay Bukharin, Petr Kapitsa regarding the role of science in society. The author also turns to the analysis of the key philosophical texts of the era, which are direct evidence of the open position of Soviet philosophers on various issues, such as the definition of science, truth, and practice, and also finds out the specific influence of the doctrine of communism on the idea of Soviet academicism as the only politically permissible and at the same time as supposedly the only scientifically possible academic position. For this purpose, the author primarily analyzes the texts of the Soviet “Philosophical Encyclopedia”. The author also refers to critical sources that analyze the Soviet era – the works of Alain Besançon, Nelli Motroshilova, Anatolii Yermolenko and Serhii Yosypenko, Evert van der Zweerde and others. The author also gives his own critical assessment of the presence of vestiges of Soviet academicism in the modern Ukrainian academic sphere.

Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons.

It is widely believed that information storage in neuronal circuits involves nanoscopic structural changes at synapses, resulting in the formation of synaptic engrams. However, direct evidence for this hypothesis is lacking. To test this conjecture, we combined chemical potentiation, functional analysis by paired pre-postsynaptic recordings, and structural analysis by electron microscopy (EM) and freeze-fracture replica labeling (FRL) at the rodent hippocampal mossy fiber synapse, a key synapse in the trisynaptic circuit of the hippocampus. Biophysical analysis of synaptic transmission revealed that forskolin-induced chemical potentiation increased the readily releasable vesicle pool size and vesicular release probability by 146% and 49%, respectively. Structural analysis of mossy fiber synapses by EM and FRL demonstrated an increase in the number of vesicles close to the plasma membrane and the number of clusters of the priming protein Munc13-1, indicating an increase in the number of both docked and primed vesicles. Furthermore, FRL analysis revealed a significant reduction of the distance between Munc13-1 and CaV2.1 Ca2+ channels, suggesting reconfiguration of the channel-vesicle coupling nanotopography. Our results indicate that presynaptic plasticity is associated with structural reorganization of active zones. We propose that changes in potential nanoscopic organization at synaptic vesicle release sites may be correlates of learning and memory at a plastic central synapse.

Faunal Exploitation Strategies, Dietary Habits, and Social Status in Tang Dynasty Chang'an: Zooarchaeological Insights From Xiajiazhuang

ABSTRACTZooarchaeology in China has consistently enhanced our understanding of past environments and human–animal interactions. However, this research has predominantly focused on prehistoric contexts. Our knowledge of animal exploitation strategies in historical periods remains largely reliant on written and iconographic sources, lacking direct and quantifiable faunal evidence. This paper examines the animal remains found in the residential district of Xiajiazhuang to better understand faunal exploitation strategies, dietary habits, and meat procurement patterns in Chang'an (Xi'an, China) during the Tang dynasty period (618–907 ce). Zooarchaeological evidence identifies caprines as the primary meat source, supplemented by cattle, dogs, pigs, and poultry. Our findings indicate a targeted meat procurement strategy, with animals selected based on their age to secure high‐quality meat, thereby hinting at the potential high status of Xiajiazhuang's residents. Along with other lines of evidence, our results suggest that interactions within the city and between the capital and its surrounding areas could have played a significant role in shaping faunal resource management strategies in Xiajiazhuang during the Tang dynasty.

Anterior piriform cortex dysfunction underlies autism spectrum disorders-related olfactory deficits in Fmr1 conditional deletion mice.

Previous studies indicated that ASD-related olfactory dysfunctions are rooted in the piriform cortex. However, the direct evidence supporting a causal link between the dysfunction of the piriform cortex and olfactory disorders in ASD is limited. In the present study, we explored the role of anterior piriform cortex (aPC) in ASD-related olfactory disorders by specifically ablating Fmr1, a leading known monogenic cause for ASD, in the pyramidal neurons. Our data demonstrated that the targeted deletion of Fmr1 in aPC pyramidal neurons was sufficient to induce deficits in olfactory detection. In vivo and in vitro electrophysiological recordings showed that the deletion of Fmr1 increased the activity of pyramidal neurons, exhibiting an enhanced excitatory response and a reduced inhibitory response upon odor stimulation. Furthermore, specific deletion of Fmr1 enhanced the power of beta oscillations during odor stimuli, meanwhile, disturbed excitatory and inhibitory synaptic transmission. The abnormal morphology of pyramidal neurons induced by the deletion of Fmr1 may be responsible for the impaired aPC neuronal function. These findings suggest that dysfunction of the aPC may play a role in olfactory impairments observed in ASD models related to Fmr1 deficiency.