The study offers an evaluation of seismic response and vulnerability for a five-story laminated bamboo frame structure, assessing the effectiveness of seismic intensity measures across various damage thresholds and the modeling uncertainties involved. A structural finite element model was developed in OpenSees software, utilizing the Pinching4 model to accurately represent the non-linear characteristics of T-shaped steel connections in beam-column joints. Two collections of earthquake records, one consisting of pulse-like near-field and the other of far-field events, were utilized to examine the structure's nonlinear behavior. Additionally, vulnerability curves for various damage states were generated using the multiple stripe analysis technique, applied to both ground motion datasets. The applicability of 24 seismic intensity measures was also analyzed across various damage limit states. The modeling uncertainty under different seismic actions was further estimated using a first-order second-moment method. Ultimately, the annual collapse probability of the structure are elucidated. Research indicates that due to the influence of joint stiffness, the overall structure demonstrates dynamic characteristics that are predominantly of the first mode shape. The spectral acceleration associated with the fundamental period achieves a reduced logarithmic standard deviation in the vulnerability analysis across various limit states. Other IMs considering higher mode effects or softening period actions no longer predominate. In most cases, the modeling uncertainty under far-field seismic action is higher than that under near-field, especially at the severe damage limit states by using an efficient intensity measure. The laminated bamboo frame structure faces a much higher collapse risk in near-field than in far-field conditions.