Background: Ultrasound (US) is now used as an imaging modality for the evaluation of lower uterine segment (LUS). A number of reports of sonographic evaluation of LUS have appeared in literature. It has been speculated that thickness of the LUS is related to the quality of wound healing. There is strong correlation between the anatomic status of scarred LUS as assessed by USG and its functional status which is tested during labour. Objective: The aim of this work was to investigate the accuracy of two-dimensional and three-dimensional ultrasound in measurement of Caesarean section scar thickness. Patients and methods: This study was cross sectional study, conducted in Aswan University Hospital. The ultrasound examinations were conducted in the Obstetrics and Gynecology department between June 2017 and September 2021. The study included 150 pregnant females with previous one or two cesarean sections (CS) (aged 20-41y; mean 32.5y), underwent TA 2D and 3D US for assessment of the LUS thickness at 37-39 gestational weeks, before planning.