This paper focuses on the current issues of the new banking regulation and its expected impact on the banking sector in the Czech Republic. Its aim is to provide current information on the basic regulatory proposals issued under Basel III and their potential impact on the functioning of the banking sector in the CR and then on real economic development in the Czech Republic. The paper will analyze the basic characteristics of the banking sector in the CR and the current situation with regard to the stability of the European banking sector, which are relevant for setting regulatory measures. Finally, the paper will examine the potential effects of the essential elements of the new banking regulation on the functioning of the banking sector in the CR. More detailed research shows that the CR´s banking sector is ready for adopting the new banking regulation and a fundamental change in its operation with signifi cant impacts on the economy is not expected.