Eucerin Research Articles

The Topical Effect of Cappariss spinosa Extract on Burn Wound Healing

Background: Skin burn is one of the most common complications and it still is considered as a major public health issue throughout the world. Objectives: This experiment was conducted in order to study the effects of Capparis spinosa leaves extract (CSLE) cream compared with silver sulfadiazine (SSD) cream to heal burn wounds in rats. Methods: Different parts of the plant (fruit, leaves and petals) were examined for antioxidant activity by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay and leave extract was chosen for preparation CSLE cream. Ninety adult male Wistar rats were randomly divided into six groups; each group consists of 15 animals. After sedation, a metal heated in boiling water was used for type II skin burning in 1.5 cm diameter circle on the back of the rats. Group one was not treated and considered as control. The burned areas in 2 - 6 groups were covered twice a day with eucerin, SSD cream and CSLE cream (2.5, 5 and 10 %), respectively. The percentage of the burn wound contraction and histopathological examinations on days 4, 9, and 14 were regarded as the parameters of our study. The obtained data were compared between the groups and days, statistically. Results: Our finding showed that 5 and 10% CSLE cream are more effective to decrease burn wound area compared with other groups. Additionally, there was no significant difference between group 5 and 10% regarding wound-size reduction (P < 0.05). Therefore, 5% CSLE cream is the most effective for wound healing. Conclusions: This study indicated that Capparis spinosa leaves extract has potential effect to accelerate wound healing and decrease inflammation in the treated rats and it can be applied in the future, clinically.

The effect of Lactobacillus brevis isolated from Iranian traditional cheese on cutaneous wound healing in rats

Today, there is a great interest in healing cutaneous wounds. Probiotics are defined as different microorganisms that may have positive effects on preventing or treating special pathologic conditions. It is also possible to use probiotics in wound healing processes. Since much research has not been done on probiotics, this study is designed to investigate the effects of Lactobacillus brevis on cutaneous wound healing. Twenty two strains of lactobacillus isolated from traditional dairy products of Iran are investigated for exopolysaccharide (EPS) production applying the phenol-sulfuric acid method.L. brevis selected for its high exopolysaccharide (EPS) and mucoid coloniese production. A full-thickness wound (1.5 × 1.5 cm) was made on the back of each rat (45 rats in 3 groups). Two groups of holding control and experimental ones treated by eucerin and eucerin containing L. brevis. Additionally, negative control groups did not receive anything. On days 1, 3, 7, 14 and 21 rats were killed and wound samples were collected for histological and statistical studies. On day 21, The percentage of wound healing (99.53%) and inflammation in the experimental groups compared with the control (90.55%) and negative control groups (91.14%) were significant (p<0.001). In contrast to the control and negative control groups, the number of neutrophils and macrophages in the experimental groups showed a faster reduction (p<0.01). The current study presented a significant decrease in inflammation and an acceleration of wound healing in L. brevis treated rats as compared to the control and negative control groups. Further studies are required to develop a detailed mechanism of L. brevis during cutaneous wound healing. Key words: Cutaneous wound, exopolysaccharide, healing, Lactobacillus brevis, probiotic.

The Study of Hypericum perforatum extract on wound healing in rabbit

Wound healing is a natural reaction to injury witch results in restoration of tissue integrity. Repair consists of four phases: inflammation, re-epithelialization, angiogenesis, fibroblast recruitment and scarring. In this study the healig effect of Hypericum perforatum extract was investigated on open wound. Hydroalcoholic extract of Hypericum P. was prepared by maceration method. Newzeland rabbits of either sexes weighting 1.6-2.5 kg were used. Method of Cross et al (1995) was used to perform excisional wound. Hairs of lower back and left flank of animal was carefully shaved and cleaned. Animal was held in standard crouching position. A template measuring 20×20 mm was placed on the skin and the outline traced using a fixed-tipped pen. Desired area was locally anasthetised with lidocaine 2%. Full thickness wound was made by excising the skin using a scalped blade and forceps. Every day the outline of each wound was traced by using a transparent plastic sheet. Treatment of wounds started from the first moments, twice daily. In other to minimize the errors, measurment was repeated three times. Then area of wounds was calculated by mm chart paper. Wound of bland group were left untreated. Second group was treated with eucerin as placebo control, in third group phenytion cream 1% was used as standard control and in other groups different concentration of hypericum extract 2%, 5%, and 10% w/w in eucerin base were adminstrated twice daily. Area of wound in the first day was considerd as 100% and area of subequent days were compared with initial day. Percentage of healing was calculared by substracting the percentage of area from 100. Healing of eucerin and phenytoin groups lasted 21 and 158 day respectively. Healing in Hypericum cream of 2%, 5% and 10% groups lasted 14, 15 and 16 days respectively. Best results obtained with 2% extract. The result of this study confirms that Hypericum cream 2% had the best wound healing effect even better than phenytion cream, however the Hypericum Cream 5% & 10% had good and noticeable effect too.